Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000121

Physical Location & Seq

Genomic Coordinates Reverse (-)

27891289 .. 27894000

Gene Structure 2712 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ44672

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2484 bp

ATGATAGTACTAGTCAAAGTCTATATCTTGACTGCTGTTGATCATTCACCACCTATCTACACTCCTGTGGAAGACATCACTGTCACATGTGGCTTTTCCGGCAACCAATTCAACAGGTATGATAACCAAACTTGGAGTGGAGATGTCAATTCCATATTCTCCCCCATAGTTGGTAACACGTCCCAAGTCAGAAAAGCACCACATTCATCCTCCTCCACCGCCAGCCATGTGCCTTACACTACAGCGCGGCTTTCTAAATCCAGTTTTACAAACCGATTTAATCTCACTACCGGCCAAAAGTTCATTCCCTTGTATTTCTATCCTGTTTCCTATGCCAACTTCTACCGCTCCAAATCTCTCTTCTCCGTCAAAGCCGGTATCTTTGCCCTTCTTGACGATTTCAATCCTTTTGTCACTGCGGATGATAATGGGGTGGAGACGCTATATAGAGAATTTTGTTTAAACATTGGCGACGAACAGAGTCTAAACATCACCTTCAGTCCAAGCACAGATGCATATGCATTTATCAACGGAATAGAAATTGTCTCGATGCCAACCAATCTTTACTACTCTCCATACAAAAACCACCGAATTGGAATTACCTCGGCCATAGAAAATAACACTGCTCTGGAGATGGTCTACCGAATCAACATTGGTGGAAGCTCACTCTCATTCAATGAAGACACTGGCATGTACCGCAATTGGGAAGGTGAGGACGAAACCTACTTGGATGATTTCAGCTTACATTTCAGCATTCTACAGCAAAATAGTAGCTCTGAACTCCATTTTGCCACAATACCCGAGTACACAGCACCAGCTGAAGTGTACCAAACGCGCCGATCGATGGGGATGAACAAAACCATCAACAAGAGCTACAATCTCACCTGGATATTTCCTGTTGATTCTAATTTTAATTACCTCATTAGGCTGCATTTTTGTGAGTTTCAACCAGATATTACTCGGTATGGAGACCGAAGATTTCAAATCTACATAGATAGTGAGAACGTTGAAGAAGGAGCAGATGTAATCCAATGGAGTGGTGGAAATGGGATTCCAGTGTACAGAGACTACACAGTCTTCATGTCGAGCGTTAAAGATGAAAGGAAAGTAAATCTTTTTCTCACGCTTGCAGCATTCCAAAAAGATTGGCTGACTTCATACAACGATGGAATCCTGAATGGGATAGAAATCTTCAAATTAAATGACTCAGATGGCTCATATAAGGCCCGAACGTTTTTCTATATCGATTCACGTCCTATACCACCGCAAGTGAAAAGACAGAAAAGCCAAAATAACTCTACTATGCTTACCATTGTTACGAGTGTAGTTTCAAGCATACTTGCGCTCTCTGTCATCGGATTCTTGGCCTTCAGGCGAGGATGGAAAGTTAAGTACTCTGGATCCAGCCACGAGGCAAAGAAGTCAATGCAGACCCATAGGTCATCTTTACCGACTTATATGTGTCGTTATTTTTCACTGGCTGAGATCAAAGCTGCCACTAAAAACTTTAATTTTAGAATTGGAGTTGGAGGGTTTGGCAATGTCTACAAAGGATGGATTGATAGTGGGGCAACCCTTGTTGTAATCAAACGGCTGAAACCAGATTCATCACAGGGTGCTCACGAGTTTAAGACCGAAATTGAGATGCTCTCTCAACTCCGTCATCGCCATTTGGTGTCTCTGATTGGATATTGTGATGATGAAGGTGAGATGATTTTGGTGTATGATTACATGGCATGTGGGACCCTCAGCGATCATCTAGACCAGACCAATAATCCACCTCTTCCATGGGAACATCGACTTCAGATTTGTATTGGCGCAGCACGAGGGTTGCAGTACCTCCACAGTGGTACAAGGGGCACCATCATACACCGTGATGTGAAGAGCACAAACATCTTATTGGATGACAAATGGGTGGCCAAAGTTTCGGATTTTGGCTTGTCCAAGGGCACCACTAACATGTCCAAAACACACATCAGCACTCTGGTGAAAGGCAGTTTCGGGTATCTGGACCCAGAATATTACCGACGACGACGTCTGACCGAGAAGTCAGACGTTTACTCCTTTGGTGTAGTGTTGTGTGAAGTGTTGTGTGCGAGGCCAGCTGTGATAAATGCGGAGGAAATTAGGCAAGCTAGCCTTGCTGAGTGGGCCAAGAGCTGTTACCGGAATGGGGAACTTGATCAAATCATTGATCCAAGTCTGAGAGGTAAGATTGCAACTGAGTGCCTGAATAAGTTCACTGAGGTAGCTATCAGTTGCATGCATGACAATGGAATAGAACGACCATCCATGACTGATGTTGTTAGGGACCTAGAACTTGCATTGCAGCTTCAGCAGAGAGCAGAAGGGAATATCAACTGCACTGAAAGGAATGTTGAAACTGAAACAAGCAGTAGTGAGCAAAGTTGTGCCACCAACGATTCCATTAAATGTATATCAGCAACTATCTTCTCTGAGATTAACAACCCAAGTGGAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009723 GO:0009725 GO:0009741 GO:0009787 GO:0009788 GO:0009791 GO:0009856 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010118 GO:0010483 GO:0010646 GO:0010648 GO:0014070 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022622 GO:0023051 GO:0023057 GO:0030054 GO:0030308 GO:0030312 GO:0032501 GO:0032502 GO:0033993 GO:0036211 GO:0040008 GO:0042221 GO:0043170 GO:0043207 GO:0043412 GO:0043680 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044426 GO:0044462 GO:0044464 GO:0044703 GO:0044706 GO:0045926 GO:0046777 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051128 GO:0051703 GO:0051704 GO:0051707 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:0098542 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1905957 GO:1905958

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Malectin_like Pkinase_Tyr

Protein Analysis

827

Amino Acids

93.42

Weight (kDa)

6.18

Isoelectric Point (pI)

45.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Malectin_like	PF12819	29 - 399	1.6e-29	Malectin-like domain
Malectin	PF11721	213 - 359	5.3e-11	Malectin domain
PK_Tyr_Ser-Thr	PF07714	506 - 772	3e-49	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	506 - 770	7.8e-42	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000121)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g46570 FvH4_6g46580 FvH4_6g46610 FvH4_6g46620 FvH4_6g46640 FvH4_6g46680
malus_domestica	MD03G1063600.v1.1 MD03G1063700.v1.1 MD09G1069300.v1.1 MD09G1069400.v1.1 MD11G1067200.v1.1 MD11G1070300.v1.1 MD11G1153300.v1.1 MD17G1060300.v1.1 MD17G1061900.v1.1 MD17G1062100.v1.1 MD17G1062300.v1.1 MD17G1062400.v1.1 MD17G1063000.v1.1 MD17G1063200.v1.1 MD17G1063600.v1.1
prunus_persica	Prupe.3G246200_v2.0.a1 Prupe.3G251000_v2.0.a1 Prupe.3G251100_v2.0.a1 Prupe.3G251200_v2.0.a1 Prupe.3G251600_v2.0.a1 Prupe.3G251700_v2.0.a1 Prupe.3G251800_v2.0.a1 Prupe.3G251900_v2.0.a1
pyrus_communis	pycom03g05070 pycom03g05100 pycom12433g00070 pycom17g06000 pycom17g06170 pycom17g06180 pycom17g06200 pycom17g06210 pycom17g06280 pycom17g06300 pycom17g06310 pycom17g06320 pycom17g06330 pycom17g06350 pycom17g06370 pycom17g06380 pycom17g06400
rosa_chinensis	RchiOBHm_Chr1g0321851 RchiOBHm_Chr1g0321911 RchiOBHm_Chr1g0323951 RchiOBHm_Chr1g0361941 RchiOBHm_Chr2g0164711 RchiOBHm_Chr2g0165301 RchiOBHm_Chr2g0165311 RchiOBHm_Chr2g0165321 RchiOBHm_Chr2g0165351 RchiOBHm_Chr2g0165431 RchiOBHm_Chr2g0165461 RchiOBHm_Chr2g0165491 RchiOBHm_Chr2g0165511 RchiOBHm_Chr2g0165521 RchiOBHm_Chr2g0165531 RchiOBHm_Chr3g0481631 RchiOBHm_Chr3g0481801 RchiOBHm_Chr3g0481811 RchiOBHm_Chr3g0481821 RchiOBHm_Chr3g0481831 RchiOBHm_Chr4g0400291 RchiOBHm_Chr4g0400371 RchiOBHm_Chr4g0400391 RchiOBHm_Chr6g0274711 RchiOBHm_Chr6g0274721 RchiOBHm_Chr7g0211011
rosa_laevigata	RLG00000002260 RLG00000002262 RLG00000002263 RLG00000003027 RLG00000003028 RLG00000009196 RLG00000009197 RLG00000012573 RLG00000020400 RLG00000021523 RLG00000021524 RLG00000021525 RLG00000021527 RLG00000021528 RLG00000021529 RLG00000021530 RLG00000021532 RLG00000021533 RLG00000021534 RLG00000021535 RLG00000021537 RLG00000023384 RLG00000027681 RLG00000027682 RLG00000027685 RLG00000030240 RLG00000030401
rosa_multiflora	Rmu_co7998078.1_g000001 Rmu_co8009032.1_g000001 Rmu_co8326071.1_g000001 Rmu_co8333709.1_g000001 Rmu_co8362007.1_g000001 Rmu_co8369261.1_g000001 Rmu_co8391219.1_g000001 Rmu_co8456951.1_g000001 Rmu_co8461523.1_g000001 Rmu_sc0000427.1_g000009 Rmu_sc0000427.1_g000032 Rmu_sc0000427.1_g000033 Rmu_sc0000521.1_g000005 Rmu_sc0000521.1_g000008 Rmu_sc0000521.1_g000009 Rmu_sc0000655.1_g000016 Rmu_sc0000655.1_g000017 Rmu_sc0000655.1_g000018 Rmu_sc0000675.1_g000003 Rmu_sc0000795.1_g000018 Rmu_sc0000795.1_g000083 Rmu_sc0001306.1_g000042 Rmu_sc0001306.1_g000043 Rmu_sc0001873.1_g000001 Rmu_sc0001873.1_g000010 Rmu_sc0002529.1_g000002 Rmu_sc0002529.1_g000004 Rmu_sc0003410.1_g000004 Rmu_sc0003909.1_g000010 Rmu_sc0004097.1_g000001 Rmu_sc0005425.1_g000003 Rmu_sc0005425.1_g000004 Rmu_sc0005425.1_g000010 Rmu_sc0005425.1_g000012 Rmu_sc0005425.1_g000017 Rmu_sc0006223.1_g000014 Rmu_sc0006399.1_g000007 Rmu_sc0010324.1_g000001 Rmu_sc0010409.1_g000005 Rmu_sc0013532.1_g000005 Rmu_sc0016601.1_g000002 Rmu_sc0016601.1_g000003 Rmu_sc0016601.1_g000006 Rmu_sc0017773.1_g000001 Rmu_sc0017773.1_g000002 Rmu_sc0019514.1_g000002 Rmu_sc0021575.1_g000001 Rmu_sc0023720.1_g000002 Rmu_sc0030898.1_g000001 Rmu_sc0033982.1_g000001 Rmu_sc0034651.1_g000001 Rmu_sc0038482.1_g000001 Rmu_sc0038644.1_g000001 Rmu_sc0038644.1_g000003 Rmu_sc0039243.1_g000001 Rmu_sc0042715.1_g000001 Rmu_ssc0000124.1_g000003
rosa_roxburghii	Rroxscaffold_2G00085740 Rroxscaffold_2G00085760 Rroxscaffold_2G00085780 Rroxscaffold_2G00085800 Rroxscaffold_2G00085830 Rroxscaffold_2G00085850 Rroxscaffold_2G00085860 Rroxscaffold_4G00294610 Rroxscaffold_4G00326070 Rroxscaffold_4G00326080 Rroxscaffold_4G00327420 Rroxscaffold_4G00327430 Rroxscaffold_4G00327460 Rroxscaffold_4G00327470 Rroxscaffold_5G00345350 Rroxscaffold_6G00399890 Rroxscaffold_6G00400250 Rroxscaffold_7G00194000 Rroxscaffold_7G00194020 Rroxscaffold_7G00194050
rosa_rugosa	Rorug01G0034300 Rorug01G0034500 Rorug01G0040700 Rorug01G0064300 Rorug02G0513700 Rorug02G0513800 Rorug02G0513900 Rorug02G0514000 Rorug02G0514000 Rorug02G0514100 Rorug02G0514300 Rorug02G0514400 Rorug02G0514700 Rorug02G0515000 Rorug02G0515100 Rorug02G0515300 Rorug02G0515500 Rorug02G0515600 Rorug02G0515800 Rorug02G0515900 Rorug02G0516000 Rorug03G0193200 Rorug04G0024600 Rorug06G0083100 Rorug06G0085200 Rorug07G0188600
rosa_samantha	Rh1CG234000 Rh2DG603700 Rh2DG603800 Rh2DG603900 Rh6BG203000
rosa_wichuraiana	Rw0G010290 Rw1G004240 Rw1G005000 Rw1G026960 Rw2G048310 Rw2G048320 Rw2G048330 Rw2G048350 Rw2G048360 Rw2G048380 Rw2G048430 Rw2G048460 Rw2G048500 Rw2G048510 Rw2G048520 Rw2G048530 Rw3G021810 Rw4G008270 Rw6G017320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	5 cut(s)	80, 1073, 1438, 2034, 2047
AatII	GACGTC	1 cut(s)	2038
AccB1I	GGYRCC	2 cut(s)	1859, 1949
AccBSI	CCGCTC	1 cut(s)	348
AccI	GTMKAC	2 cut(s)	639, 1545
AccII	CGCG	2 cut(s)	247, 835
AciI	CCGC	7 cut(s)	219, 247, 346, 419, 697, 1265, 2117
AclI	AACGTT	2 cut(s)	1005, 1232
AclWI	GGATC	3 cut(s)	1395, 1408, 2189
AcoI	YGGCCR	3 cut(s)	292, 606, 1917
AcsI	RAATTY	1 cut(s)	452
AcuI	CTGAAG	5 cut(s)	481, 840, 1354, 1787, 2318
AcyI	GRCGYC	1 cut(s)	2035
AdeI	CACNNNGTG	1 cut(s)	1616
AfaI	GTAC	8 cut(s)	9, 695, 806, 827, 1061, 1394, 1838, 1852
AfiI	CCNNNNNNNGG	4 cut(s)	170, 703, 844, 2173
AflIII	ACRYGT	3 cut(s)	86, 177, 1959
AgsI	TTSAA	9 cut(s)	112, 403, 676, 947, 983, 1010, 1195, 1332, 2381
AhlI	ACTAGT	1 cut(s)	10
AjiI	CACGTC	2 cut(s)	180, 1253
AjnI	CCWGG	1 cut(s)	884
AjuI	GAANNNNNNNTTGG	4 cut(s)	288, 320, 344, 376
AleI	CACNNNNGTG	2 cut(s)	65, 1985
Alw21I	GWGCWC	2 cut(s)	1621, 1889
Alw26I	GTCTC	5 cut(s)	431, 550, 963, 1059, 1683
AlwI	GGATC	3 cut(s)	1395, 1408, 2189
Ama87I	CYCGRG	1 cut(s)	800
AoxI	GGCC	7 cut(s)	292, 606, 1224, 1365, 1917, 2099, 2151
ApeKI	GCWGC	5 cut(s)	928, 1130, 1493, 1820, 2329
ApoI	RAATTY	1 cut(s)	452
ArsI	GACNNNNNNTTYG	2 cut(s)	1791, 1823
Asp700I	GAANNNNTTC	1 cut(s)	2237
AspLEI	GCGC	4 cut(s)	247, 837, 1345, 1820
AspS9I	GGNCC	5 cut(s)	1225, 1743, 2011, 2151, 2311
AsuHPI	GGTGA	6 cut(s)	39, 484, 722, 874, 1718, 1999
AsuNHI	GCTAGC	1 cut(s)	2135
AvaI	CYCGRG	1 cut(s)	800
AvaII	GGWCC	3 cut(s)	1743, 2011, 2311
BaeGI	GKGCMC	2 cut(s)	1862, 1952
BalI	TGGCCA	1 cut(s)	1919
BamHI	GGATCC	1 cut(s)	1400
BanI	GGYRCC	2 cut(s)	1859, 1949
BauI	CACGAG	3 cut(s)	1409, 1622, 1824
BbsI	GAAGAC	3 cut(s)	78, 687, 1069
Bbv12I	GWGCWC	2 cut(s)	1621, 1889
BbvCI	CCTCAGC	1 cut(s)	1748
BbvI	GCAGC	5 cut(s)	915, 1142, 1480, 1832, 2341
BccI	CCATC	9 cut(s)	628, 838, 869, 1160, 1205, 1374, 1548, 1871, 2296
BceAI	ACGGC	1 cut(s)	1607
BciT130I	CCWGG	1 cut(s)	886
BclI	TGATCA	2 cut(s)	40, 2182
BcoDI	GTCTC	5 cut(s)	431, 550, 963, 1059, 1683
BcuI	ACTAGT	1 cut(s)	10
BfaI	CTAG	4 cut(s)	11, 1760, 2136, 2315
BfmI	CTRYAG	2 cut(s)	240, 758
BisI	GCNGC	6 cut(s)	248, 929, 1131, 1494, 1821, 2330
BlsI	GCNGC	6 cut(s)	249, 930, 1132, 1495, 1822, 2331
BmcAI	AGTACT	2 cut(s)	9, 1394
Bme1390I	CCNGG	1 cut(s)	886
Bme18I	GGWCC	3 cut(s)	1743, 2011, 2311
BmeT110I	CYCGRG	1 cut(s)	800
BmgBI	CACGTC	2 cut(s)	180, 1253
BmgT120I	GGNCC	5 cut(s)	1225, 1743, 2011, 2151, 2311
BmiI	GGNNCC	7 cut(s)	1402, 1744, 1745, 1861, 1951, 2013, 2312
BmrFI	CCNGG	1 cut(s)	886
BmsI	GCATC	3 cut(s)	502, 540, 1635
BmtI	GCTAGC	1 cut(s)	2139
BpiI	GAAGAC	3 cut(s)	78, 687, 1069
BplI	GAGNNNNNCTC	2 cut(s)	1634, 1666
BpmI	CTGGAG	1 cut(s)	650
Bpu10I	CCTNAGC	1 cut(s)	1748
Bsa29I	ATCGAT	2 cut(s)	842, 1245
BsaBI	GATNNNNATC	2 cut(s)	402, 1187
BsaHI	GRCGYC	1 cut(s)	2035
BsaI	GGTCTC	1 cut(s)	963
BsaJI	CCNNGG	3 cut(s)	603, 1787, 1944
BsaWI	WCCGGW	1 cut(s)	2166
BsaXI	ACNNNNNCTCC	4 cut(s)	1008, 1038, 2045, 2075
Bsc4I	CCNNNNNNNGG	4 cut(s)	170, 703, 844, 2173
Bse118I	RCCGGY	2 cut(s)	290, 374
Bse1I	ACTGG	4 cut(s)	261, 691, 1055, 1482
Bse3DI	GCAATG	2 cut(s)	1546, 2324
Bse8I	GATNNNNATC	2 cut(s)	402, 1187
BseBI	CCWGG	1 cut(s)	886
BseCI	ATCGAT	2 cut(s)	842, 1245
BseDI	CCNNGG	3 cut(s)	603, 1787, 1944
BseGI	GGATG	8 cut(s)	206, 427, 736, 855, 1385, 1559, 1909, 2288
BseJI	GATNNNNATC	2 cut(s)	402, 1187
BseLI	CCNNNNNNNGG	4 cut(s)	170, 703, 844, 2173
BseMI	GCAATG	2 cut(s)	1546, 2324
BseMII	CTCAG	8 cut(s)	1221, 1473, 1762, 2136, 2195, 2214, 2235, 2448
BseNI	ACTGG	4 cut(s)	261, 691, 1055, 1482
BseRI	GAGGAG	1 cut(s)	202
BseSI	GKGCMC	2 cut(s)	1862, 1952
BseXI	GCAGC	5 cut(s)	915, 1142, 1480, 1832, 2341
BsgI	GTGCAG	1 cut(s)	2347
Bsh1236I	CGCG	2 cut(s)	247, 835
Bsh1285I	CGRYCG	1 cut(s)	842
BshFI	GGCC	7 cut(s)	294, 608, 1226, 1367, 1919, 2101, 2153
BshNI	GGYRCC	2 cut(s)	1859, 1949
BshVI	ATCGAT	2 cut(s)	842, 1245
BsiEI	CGRYCG	1 cut(s)	842
BsiHKAI	GWGCWC	2 cut(s)	1621, 1889
BsiHKCI	CYCGRG	1 cut(s)	800
BsiSI	CCGG	4 cut(s)	99, 291, 375, 2167
BslFI	GGGAC	3 cut(s)	166, 1756, 2324
BslI	CCNNNNNNNGG	4 cut(s)	170, 703, 844, 2173
BsmAI	GTCTC	5 cut(s)	431, 550, 963, 1059, 1683
BsmBI	CGTCTC	1 cut(s)	431
BsmFI	GGGAC	3 cut(s)	166, 1756, 2324
BsmI	GAATGC	2 cut(s)	753, 1133
BsnI	GGCC	7 cut(s)	294, 608, 1226, 1367, 1919, 2101, 2153
Bso31I	GGTCTC	1 cut(s)	963
BsoBI	CYCGRG	1 cut(s)	800
Bsp1286I	GDGCHC	4 cut(s)	1621, 1862, 1889, 1952
Bsp1407I	TGTACA	1 cut(s)	1059
Bsp143I	GATC	7 cut(s)	40, 839, 1400, 1485, 1753, 2182, 2194
Bsp19I	CCATGG	1 cut(s)	1787
BspACI	CCGC	7 cut(s)	219, 247, 346, 419, 697, 1265, 2117
BspANI	GGCC	7 cut(s)	294, 608, 1226, 1367, 1919, 2101, 2153
BspCNI	CTCAG	8 cut(s)	1220, 1474, 1761, 2137, 2196, 2215, 2236, 2449
BspDI	ATCGAT	2 cut(s)	842, 1245
BspFNI	CGCG	2 cut(s)	247, 835
BspLI	GGNNCC	7 cut(s)	1402, 1744, 1745, 1861, 1951, 2013, 2312
BspOI	GCTAGC	1 cut(s)	2139
BspPI	GGATC	3 cut(s)	1395, 1408, 2189
BspQI	GCTCTTC	1 cut(s)	1877
BspT107I	GGYRCC	2 cut(s)	1859, 1949
BspTNI	GGTCTC	1 cut(s)	963
BsrBI	CCGCTC	1 cut(s)	348
BsrDI	GCAATG	2 cut(s)	1546, 2324
BsrFI	RCCGGY	2 cut(s)	290, 374
BsrGI	TGTACA	1 cut(s)	1059
BsrI	ACTGG	4 cut(s)	261, 691, 1055, 1482
BssAI	RCCGGY	2 cut(s)	290, 374
BssECI	CCNNGG	3 cut(s)	603, 1787, 1944
BssMI	GATC	7 cut(s)	40, 839, 1400, 1485, 1753, 2182, 2194
BssNI	GRCGYC	1 cut(s)	2035
BssSI	CACGAG	3 cut(s)	1409, 1622, 1824
BssT1I	CCWWGG	2 cut(s)	1787, 1944
Bst2BI	CACGAG	3 cut(s)	1409, 1622, 1824
Bst2UI	CCWGG	1 cut(s)	886
Bst4CI	ACNGT	4 cut(s)	82, 1075, 1847, 1874
Bst6I	CTCTTC	3 cut(s)	365, 1788, 1877
BstACI	GRCGYC	1 cut(s)	2035
BstAUI	TGTACA	1 cut(s)	1059
BstC8I	GCNNGC	6 cut(s)	223, 1128, 2103, 2133, 2137, 2264
BstDEI	CTNAG	8 cut(s)	1207, 1482, 1748, 2145, 2204, 2223, 2244, 2457
BstDSI	CCRYGG	1 cut(s)	1787
BstF5I	GGATG	8 cut(s)	206, 427, 736, 855, 1385, 1559, 1909, 2288
BstFNI	CGCG	2 cut(s)	247, 835
BstHHI	GCGC	4 cut(s)	247, 837, 1345, 1820
BstKTI	GATC	7 cut(s)	43, 842, 1403, 1488, 1756, 2185, 2197
BstMAI	GTCTC	5 cut(s)	431, 550, 963, 1059, 1683
BstMBI	GATC	7 cut(s)	40, 839, 1400, 1485, 1753, 2182, 2194
BstMCI	CGRYCG	1 cut(s)	842
BstMWI	GCNNNNNNNGC	6 cut(s)	99, 696, 2102, 2141, 2150, 2335
BstNI	CCWGG	1 cut(s)	886
BstNSI	RCATGY	5 cut(s)	90, 694, 1740, 1963, 2266
BstSCI	CCNGG	1 cut(s)	884
BstSFI	CTRYAG	2 cut(s)	240, 758
BstSLI	GKGCMC	2 cut(s)	1862, 1952
BstUI	CGCG	2 cut(s)	247, 835
BstV1I	GCAGC	5 cut(s)	915, 1142, 1480, 1832, 2341
BstV2I	GAAGAC	3 cut(s)	78, 687, 1069
BstX2I	RGATCY	1 cut(s)	1400
BstXI	CCANNNNNNTGG	2 cut(s)	593, 1037
BstYI	RGATCY	1 cut(s)	1400
Bsu15I	ATCGAT	2 cut(s)	842, 1245
BsuRI	GGCC	7 cut(s)	294, 608, 1226, 1367, 1919, 2101, 2153
BsuTUI	ATCGAT	2 cut(s)	842, 1245
BtgI	CCRYGG	1 cut(s)	1787
BtgZI	GCGATG	1 cut(s)	1649
BtrI	CACGTC	2 cut(s)	180, 1253
BtsCI	GGATG	8 cut(s)	206, 427, 736, 855, 1385, 1559, 1909, 2288
BtsI	GCAGTG	2 cut(s)	414, 621
BtsIMutI	CAGTG	9 cut(s)	78, 414, 621, 684, 1062, 1475, 1852, 2241, 2364
Cac8I	GCNNGC	6 cut(s)	223, 1128, 2103, 2133, 2137, 2264
CfoI	GCGC	4 cut(s)	247, 837, 1345, 1820
Cfr10I	RCCGGY	2 cut(s)	290, 374
Cfr13I	GGNCC	5 cut(s)	1225, 1743, 2011, 2151, 2311
ClaI	ATCGAT	2 cut(s)	842, 1245
CseI	GACGC	1 cut(s)	448
Csp6I	GTAC	8 cut(s)	8, 694, 805, 826, 1060, 1393, 1837, 1851
CspCI	CAANNNNNGTGG	3 cut(s)	637, 672, 2455
CviQI	GTAC	8 cut(s)	8, 694, 805, 826, 1060, 1393, 1837, 1851
DdeI	CTNAG	8 cut(s)	1207, 1482, 1748, 2145, 2204, 2223, 2244, 2457
DpnI	GATC	7 cut(s)	42, 841, 1402, 1487, 1755, 2184, 2196
DpnII	GATC	7 cut(s)	40, 839, 1400, 1485, 1753, 2182, 2194
DraI	TTTAAA	1 cut(s)	462
DraIII	CACNNNGTG	1 cut(s)	1616
DrdI	GACNNNNNNGTC	5 cut(s)	80, 1073, 1438, 2034, 2047
DseDI	GACNNNNNNGTC	5 cut(s)	80, 1073, 1438, 2034, 2047
EaeI	YGGCCR	3 cut(s)	292, 606, 1917
Eam1104I	CTCTTC	3 cut(s)	365, 1788, 1877
EarI	CTCTTC	3 cut(s)	365, 1788, 1877
Eco130I	CCWWGG	2 cut(s)	1787, 1944
Eco31I	GGTCTC	1 cut(s)	963
Eco47I	GGWCC	3 cut(s)	1743, 2011, 2311
Eco57I	CTGAAG	5 cut(s)	481, 840, 1354, 1787, 2318
Eco88I	CYCGRG	1 cut(s)	800
EcoO109I	RGGNCCY	2 cut(s)	1743, 2311
EcoRII	CCWGG	1 cut(s)	884
EcoT14I	CCWWGG	2 cut(s)	1787, 1944
EcoT22I	ATGCAT	3 cut(s)	517, 523, 2268
ErhI	CCWWGG	2 cut(s)	1787, 1944
Esp3I	CGTCTC	1 cut(s)	431
FalI	AAGNNNNNCTT	4 cut(s)	1098, 1130, 2124, 2156
FaqI	GGGAC	3 cut(s)	166, 1756, 2324
FauNDI	CATATG	1 cut(s)	517
FbaI	TGATCA	2 cut(s)	40, 2182
FblI	GTMKAC	2 cut(s)	639, 1545
Fnu4HI	GCNGC	6 cut(s)	248, 929, 1131, 1494, 1821, 2330
FokI	GGATG	8 cut(s)	193, 434, 743, 862, 1392, 1566, 1916, 2275
Fsp4HI	GCNGC	6 cut(s)	248, 929, 1131, 1494, 1821, 2330
FspBI	CTAG	4 cut(s)	11, 1760, 2136, 2315
GlaI	GCGC	4 cut(s)	246, 836, 1344, 1819
GluI	GCNGC	6 cut(s)	248, 929, 1131, 1494, 1821, 2330
GsuI	CTGGAG	1 cut(s)	650
HaeIII	GGCC	7 cut(s)	294, 608, 1226, 1367, 1919, 2101, 2153
HapII	CCGG	4 cut(s)	99, 291, 375, 2167
HgaI	GACGC	1 cut(s)	448
HhaI	GCGC	4 cut(s)	247, 837, 1345, 1820
Hin1I	GRCGYC	1 cut(s)	2035
Hin6I	GCGC	4 cut(s)	245, 835, 1343, 1818
HinP1I	GCGC	4 cut(s)	245, 835, 1343, 1818
HpaII	CCGG	4 cut(s)	99, 291, 375, 2167
HphI	GGTGA	6 cut(s)	39, 484, 722, 874, 1718, 1999
Hpy166II	GTNNAC	7 cut(s)	640, 807, 826, 1060, 1546, 2059, 2241
Hpy188III	TCNNGA	9 cut(s)	28, 392, 547, 629, 1174, 1398, 1622, 1760, 2009
Hpy8I	GTNNAC	7 cut(s)	640, 807, 826, 1060, 1546, 2059, 2241
Hpy99I	CGWCG	4 cut(s)	476, 2031, 2034, 2037
HpyAV	CCTTC	7 cut(s)	398, 505, 701, 1007, 1378, 1697, 2342
HpyCH4III	ACNGT	4 cut(s)	82, 1075, 1847, 1874
HpyCH4IV	ACGT	6 cut(s)	179, 1005, 1232, 1252, 2035, 2055
HpyF10VI	GCNNNNNNNGC	6 cut(s)	99, 696, 2102, 2141, 2150, 2335
HpyF3I	CTNAG	8 cut(s)	1207, 1482, 1748, 2145, 2204, 2223, 2244, 2457
HpySE526I	ACGT	6 cut(s)	179, 1005, 1232, 1252, 2035, 2055
Hsp92I	GRCGYC	1 cut(s)	2035
HspAI	GCGC	4 cut(s)	245, 835, 1343, 1818
KflI	GGGWCCC	1 cut(s)	1743
Ksp22I	TGATCA	2 cut(s)	40, 2182
Kzo9I	GATC	7 cut(s)	40, 839, 1400, 1485, 1753, 2182, 2194
LguI	GCTCTTC	1 cut(s)	1877
LmnI	GCTCC	2 cut(s)	353, 1016
Lsp1109I	GCAGC	5 cut(s)	915, 1142, 1480, 1832, 2341
LweI	GCATC	3 cut(s)	502, 540, 1635
MaeI	CTAG	4 cut(s)	11, 1760, 2136, 2315
MaeII	ACGT	6 cut(s)	179, 1005, 1232, 1252, 2035, 2055
MaeIII	GTNAC	5 cut(s)	82, 173, 412, 1315, 2162
MalI	GATC	7 cut(s)	42, 841, 1402, 1487, 1755, 2184, 2196
MbiI	CCGCTC	1 cut(s)	348
MboI	GATC	7 cut(s)	40, 839, 1400, 1485, 1753, 2182, 2194
MfeI	CAATTG	1 cut(s)	700
MflI	RGATCY	1 cut(s)	1400
MhlI	GDGCHC	4 cut(s)	1621, 1862, 1889, 1952
MlsI	TGGCCA	1 cut(s)	1919
MluNI	TGGCCA	1 cut(s)	1919
MlyI	GAGTC	2 cut(s)	490, 1199
MmeI	TCCRAC	1 cut(s)	1507
Mox20I	TGGCCA	1 cut(s)	1919
Mph1103I	ATGCAT	3 cut(s)	517, 523, 2268
MroXI	GAANNNNTTC	1 cut(s)	2237
MscI	TGGCCA	1 cut(s)	1919
MslI	CAYNNNNRTG	7 cut(s)	65, 689, 936, 1875, 1958, 1985, 2271
Msp20I	TGGCCA	1 cut(s)	1919
MspA1I	CMGCKG	2 cut(s)	818, 2105
MspI	CCGG	4 cut(s)	99, 291, 375, 2167
MspR9I	CCNGG	1 cut(s)	886
MssI	GTTTAAAC	1 cut(s)	462
MunI	CAATTG	1 cut(s)	700
Mva1269I	GAATGC	2 cut(s)	753, 1133
MvaI	CCWGG	1 cut(s)	886
MvnI	CGCG	2 cut(s)	247, 835
MwoI	GCNNNNNNNGC	6 cut(s)	99, 696, 2102, 2141, 2150, 2335
NcoI	CCATGG	1 cut(s)	1787
NdeI	CATATG	1 cut(s)	517
NdeII	GATC	7 cut(s)	40, 839, 1400, 1485, 1753, 2182, 2194
NheI	GCTAGC	1 cut(s)	2135
NlaIV	GGNNCC	7 cut(s)	1402, 1744, 1745, 1861, 1951, 2013, 2312
NmeAIII	GCCGAG	1 cut(s)	584
NmuCI	GTSAC	2 cut(s)	82, 412
NsiI	ATGCAT	3 cut(s)	517, 523, 2268
NspI	RCATGY	5 cut(s)	90, 694, 1740, 1963, 2266
OliI	CACNNNNGTG	2 cut(s)	65, 1985
PaeI	GCATGC	1 cut(s)	2266
PciI	ACATGT	2 cut(s)	86, 1959
PciSI	GCTCTTC	1 cut(s)	1877
PcsI	WCGNNNNNNNCGW	1 cut(s)	839
PctI	GAATGC	2 cut(s)	753, 1133
PdmI	GAANNNNTTC	1 cut(s)	2237
PfeI	GAWTC	8 cut(s)	645, 902, 1051, 1170, 1247, 1359, 1604, 2423
PflFI	GACNNNGTC	1 cut(s)	2034
PkrI	GCNGC	6 cut(s)	249, 930, 1132, 1495, 1822, 2331
Ple19I	CGATCG	1 cut(s)	842
PleI	GAGTC	2 cut(s)	489, 1199
PmeI	GTTTAAAC	1 cut(s)	462
PpsI	GAGTC	2 cut(s)	489, 1199
PpuMI	RGGWCCY	2 cut(s)	1743, 2311
PscI	ACATGT	2 cut(s)	86, 1959
Psp1406I	AACGTT	2 cut(s)	1005, 1232
Psp5II	RGGWCCY	2 cut(s)	1743, 2311
Psp6I	CCWGG	1 cut(s)	884
PspGI	CCWGG	1 cut(s)	884
PspN4I	GGNNCC	7 cut(s)	1402, 1744, 1745, 1861, 1951, 2013, 2312
PspPI	GGNCC	5 cut(s)	1225, 1743, 2011, 2151, 2311
PspPPI	RGGWCCY	2 cut(s)	1743, 2311
PsuI	RGATCY	1 cut(s)	1400
PsyI	GACNNNGTC	1 cut(s)	2034
PvuI	CGATCG	1 cut(s)	842
PvuII	CAGCTG	2 cut(s)	818, 2105
RsaI	GTAC	8 cut(s)	9, 695, 806, 827, 1061, 1394, 1838, 1852
RsaNI	GTAC	8 cut(s)	8, 694, 805, 826, 1060, 1393, 1837, 1851
RseI	CAYNNNNRTG	7 cut(s)	65, 689, 936, 1875, 1958, 1985, 2271
SapI	GCTCTTC	1 cut(s)	1877
SatI	GCNGC	6 cut(s)	248, 929, 1131, 1494, 1821, 2330
Sau3AI	GATC	7 cut(s)	40, 839, 1400, 1485, 1753, 2182, 2194
Sau96I	GGNCC	5 cut(s)	1225, 1743, 2011, 2151, 2311
ScaI	AGTACT	2 cut(s)	9, 1394
SchI	GAGTC	2 cut(s)	490, 1199
ScrFI	CCNGG	1 cut(s)	886
SduI	GDGCHC	4 cut(s)	1621, 1862, 1889, 1952
SfaNI	GCATC	3 cut(s)	502, 540, 1635
SfcI	CTRYAG	2 cut(s)	240, 758
SinI	GGWCC	3 cut(s)	1743, 2011, 2311
SmiMI	CAYNNNNRTG	7 cut(s)	65, 689, 936, 1875, 1958, 1985, 2271
SpeI	ACTAGT	1 cut(s)	10
SphI	GCATGC	1 cut(s)	2266
SsiI	CCGC	7 cut(s)	219, 247, 346, 419, 697, 1265, 2117
SspI	AATATT	1 cut(s)	2021
SspMI	CTAG	4 cut(s)	11, 1760, 2136, 2315
StyD4I	CCNGG	1 cut(s)	884
StyI	CCWWGG	2 cut(s)	1787, 1944
TaaI	ACNGT	4 cut(s)	82, 1075, 1847, 1874
TaiI	ACGT	6 cut(s)	182, 1008, 1235, 1255, 2038, 2058
TaqI	TCGA	5 cut(s)	548, 842, 1085, 1245, 1798
TaqII	GACCGA	3 cut(s)	987, 1649, 2057
TatI	WGTACW	4 cut(s)	7, 804, 1059, 1392
TauI	GCSGC	1 cut(s)	250
TfiI	GAWTC	8 cut(s)	645, 902, 1051, 1170, 1247, 1359, 1604, 2423
TscAI	CASTG	9 cut(s)	85, 421, 628, 691, 1062, 1482, 1852, 2248, 2371
TseFI	GTSAC	2 cut(s)	82, 412
TseI	GCWGC	5 cut(s)	928, 1130, 1493, 1820, 2329
Tsp45I	GTSAC	2 cut(s)	82, 412
TspDTI	ATGAA	9 cut(s)	195, 292, 693, 866, 1069, 1113, 1146, 1596, 1716
TspGWI	ACGGA	3 cut(s)	355, 546, 1649
TspRI	CASTG	9 cut(s)	85, 421, 628, 691, 1062, 1482, 1852, 2248, 2371
Tth111I	GACNNNGTC	1 cut(s)	2034
VpaK11BI	GGWCC	3 cut(s)	1743, 2011, 2311
XapI	RAATTY	1 cut(s)	452
XbaI	TCTAGA	1 cut(s)	1759
XceI	RCATGY	5 cut(s)	90, 694, 1740, 1963, 2266
XcmI	CCANNNNNNNNNTGG	3 cut(s)	134, 1037, 1785
XmiI	GTMKAC	2 cut(s)	639, 1545
XmnI	GAANNNNTTC	1 cut(s)	2237
XspI	CTAG	4 cut(s)	11, 1760, 2136, 2315
ZraI	GACGTC	1 cut(s)	2036
ZrmI	AGTACT	2 cut(s)	9, 1394
Zsp2I	ATGCAT	3 cut(s)	517, 523, 2268

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.