receptor-like protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 2065 |
| AatII | GACGTC | 1 cut(s) | 2050 |
| AccB1I | GGYRCC | 1 cut(s) | 1880 |
| AccBSI | CCGCTC | 1 cut(s) | 826 |
| AccI | GTMKAC | 2 cut(s) | 153, 657 |
| AccII | CGCG | 2 cut(s) | 498, 1644 |
| AccIII | TCCGGA | 1 cut(s) | 1370 |
| AclWI | GGATC | 3 cut(s) | 965, 2300, 2504 |
| AcoI | YGGCCR | 6 cut(s) | 184, 364, 827, 1062, 1558, 2300 |
| AcsI | RAATTY | 9 cut(s) | 178, 236, 518, 649, 788, 977, 1398, 2215, 2252 |
| AcvI | CACGTG | 1 cut(s) | 1760 |
| AcyI | GRCGYC | 1 cut(s) | 2047 |
| AfaI | GTAC | 7 cut(s) | 584, 713, 767, 1365, 1568, 1873, 2078 |
| AfeI | AGCGCT | 1 cut(s) | 1209 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 318, 669, 955, 2475 |
| AjnI | CCWGG | 3 cut(s) | 165, 987, 2154 |
| AleI | CACNNNNGTG | 1 cut(s) | 2003 |
| AluBI | AGCT | 9 cut(s) | 195, 317, 429, 698, 850, 942, 1112, 1273, 2329 |
| AluI | AGCT | 9 cut(s) | 195, 317, 429, 698, 850, 942, 1112, 1273, 2329 |
| Alw21I | GWGCWC | 3 cut(s) | 197, 852, 1910 |
| Alw26I | GTCTC | 7 cut(s) | 530, 541, 632, 988, 1128, 1164, 1704 |
| AlwI | GGATC | 3 cut(s) | 965, 2300, 2504 |
| Ama87I | CYCGRG | 2 cut(s) | 551, 1447 |
| Aor13HI | TCCGGA | 1 cut(s) | 1370 |
| Aor51HI | AGCGCT | 1 cut(s) | 1209 |
| ApaI | GGGCCC | 1 cut(s) | 1642 |
| ApeKI | GCWGC | 1 cut(s) | 1205 |
| ApoI | RAATTY | 9 cut(s) | 178, 236, 518, 649, 788, 977, 1398, 2215, 2252 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1575 |
| AspLEI | GCGC | 3 cut(s) | 668, 1210, 1846 |
| AspS9I | GGNCC | 6 cut(s) | 734, 777, 1301, 1638, 1639, 2166 |
| AsuC2I | CCSGG | 4 cut(s) | 363, 410, 1448, 1449 |
| AvaI | CYCGRG | 2 cut(s) | 551, 1447 |
| AvaII | GGWCC | 2 cut(s) | 777, 1301 |
| BaeGI | GKGCMC | 2 cut(s) | 1642, 1883 |
| BalI | TGGCCA | 2 cut(s) | 1064, 1560 |
| BanI | GGYRCC | 1 cut(s) | 1880 |
| BanII | GRGCYC | 4 cut(s) | 35, 197, 852, 1642 |
| BbrPI | CACGTG | 1 cut(s) | 1760 |
| BbsI | GAAGAC | 1 cut(s) | 2351 |
| Bbv12I | GWGCWC | 3 cut(s) | 197, 852, 1910 |
| BbvI | GCAGC | 1 cut(s) | 1217 |
| BceAI | ACGGC | 4 cut(s) | 1628, 1835, 2108, 2287 |
| BciT130I | CCWGG | 3 cut(s) | 167, 989, 2156 |
| BciVI | GTATCC | 1 cut(s) | 1703 |
| BclI | TGATCA | 1 cut(s) | 2197 |
| BcnI | CCSGG | 4 cut(s) | 363, 410, 1448, 1449 |
| BcoDI | GTCTC | 7 cut(s) | 530, 541, 632, 988, 1128, 1164, 1704 |
| BfaI | CTAG | 4 cut(s) | 1196, 1437, 2112, 2439 |
| BfmI | CTRYAG | 2 cut(s) | 1074, 1211 |
| BfoI | RGCGCY | 1 cut(s) | 1211 |
| BfuI | GTATCC | 1 cut(s) | 1703 |
| BisI | GCNGC | 5 cut(s) | 450, 827, 1206, 1509, 1842 |
| BlsI | GCNGC | 5 cut(s) | 451, 828, 1207, 1510, 1843 |
| Bme1390I | CCNGG | 7 cut(s) | 167, 363, 410, 989, 1448, 1449, 2156 |
| Bme18I | GGWCC | 2 cut(s) | 777, 1301 |
| BmeT110I | CYCGRG | 2 cut(s) | 551, 1447 |
| BmgT120I | GGNCC | 6 cut(s) | 734, 777, 1301, 1638, 1639, 2166 |
| BmiI | GGNNCC | 6 cut(s) | 34, 736, 1303, 1639, 1640, 1882 |
| BmrFI | CCNGG | 7 cut(s) | 167, 363, 410, 989, 1448, 1449, 2156 |
| BmsI | GCATC | 6 cut(s) | 262, 266, 568, 1545, 1656, 2231 |
| BpiI | GAAGAC | 1 cut(s) | 2351 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1655, 1687 |
| BpmI | CTGGAG | 2 cut(s) | 409, 722 |
| BpuEI | CTTGAG | 1 cut(s) | 1601 |
| BpuMI | CCSGG | 4 cut(s) | 363, 410, 1448, 1449 |
| Bsa29I | ATCGAT | 1 cut(s) | 2508 |
| BsaAI | YACGTR | 1 cut(s) | 1760 |
| BsaHI | GRCGYC | 1 cut(s) | 2047 |
| BsaI | GGTCTC | 1 cut(s) | 632 |
| BsaJI | CCNNGG | 5 cut(s) | 278, 408, 1447, 2133, 2473 |
| BsaWI | WCCGGW | 6 cut(s) | 148, 758, 768, 1162, 1370, 1495 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 695, 725 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 318, 669, 955, 2475 |
| Bse118I | RCCGGY | 3 cut(s) | 125, 452, 662 |
| Bse1I | ACTGG | 4 cut(s) | 513, 628, 890, 1124 |
| Bse3DI | GCAATG | 1 cut(s) | 2274 |
| BseAI | TCCGGA | 1 cut(s) | 1370 |
| BseBI | CCWGG | 3 cut(s) | 167, 989, 2156 |
| BseCI | ATCGAT | 1 cut(s) | 2508 |
| BseDI | CCNNGG | 5 cut(s) | 278, 408, 1447, 2133, 2473 |
| BseGI | GGATG | 9 cut(s) | 105, 162, 257, 375, 805, 1106, 1591, 2168, 2287 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 318, 669, 955, 2475 |
| BseMI | GCAATG | 1 cut(s) | 2274 |
| BseMII | CTCAG | 2 cut(s) | 1288, 2052 |
| BseNI | ACTGG | 4 cut(s) | 513, 628, 890, 1124 |
| BseRI | GAGGAG | 2 cut(s) | 455, 1445 |
| BseSI | GKGCMC | 2 cut(s) | 1642, 1883 |
| BseX3I | CGGCCG | 1 cut(s) | 2300 |
| BseXI | GCAGC | 1 cut(s) | 1217 |
| Bsh1236I | CGCG | 2 cut(s) | 498, 1644 |
| Bsh1285I | CGRYCG | 4 cut(s) | 153, 684, 1046, 2303 |
| BshNI | GGYRCC | 1 cut(s) | 1880 |
| BshVI | ATCGAT | 1 cut(s) | 2508 |
| BsiEI | CGRYCG | 4 cut(s) | 153, 684, 1046, 2303 |
| BsiHKAI | GWGCWC | 3 cut(s) | 197, 852, 1910 |
| BsiHKCI | CYCGRG | 2 cut(s) | 551, 1447 |
| BsiWI | CGTACG | 1 cut(s) | 582 |
| BslFI | GGGAC | 4 cut(s) | 487, 1453, 1777, 2470 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 318, 669, 955, 2475 |
| BsmAI | GTCTC | 7 cut(s) | 530, 541, 632, 988, 1128, 1164, 1704 |
| BsmFI | GGGAC | 4 cut(s) | 487, 1453, 1777, 2470 |
| Bso31I | GGTCTC | 1 cut(s) | 632 |
| BsoBI | CYCGRG | 2 cut(s) | 551, 1447 |
| Bsp120I | GGGCCC | 1 cut(s) | 1638 |
| Bsp1286I | GDGCHC | 6 cut(s) | 35, 197, 852, 1642, 1883, 1910 |
| Bsp13I | TCCGGA | 1 cut(s) | 1370 |
| BspCNI | CTCAG | 2 cut(s) | 1287, 2053 |
| BspDI | ATCGAT | 1 cut(s) | 2508 |
| BspEI | TCCGGA | 1 cut(s) | 1370 |
| BspFNI | CGCG | 2 cut(s) | 498, 1644 |
| BspLI | GGNNCC | 6 cut(s) | 34, 736, 1303, 1639, 1640, 1882 |
| BspMAI | CTGCAG | 1 cut(s) | 1078 |
| BspPI | GGATC | 3 cut(s) | 965, 2300, 2504 |
| BspQI | GCTCTTC | 1 cut(s) | 1898 |
| BspT107I | GGYRCC | 1 cut(s) | 1880 |
| BspTNI | GGTCTC | 1 cut(s) | 632 |
| BsrBI | CCGCTC | 1 cut(s) | 826 |
| BsrDI | GCAATG | 1 cut(s) | 2274 |
| BsrFI | RCCGGY | 3 cut(s) | 125, 452, 662 |
| BsrI | ACTGG | 4 cut(s) | 513, 628, 890, 1124 |
| BssAI | RCCGGY | 3 cut(s) | 125, 452, 662 |
| BssECI | CCNNGG | 5 cut(s) | 278, 408, 1447, 2133, 2473 |
| BssNI | GRCGYC | 1 cut(s) | 2047 |
| BssT1I | CCWWGG | 1 cut(s) | 2473 |
| Bst2UI | CCWGG | 3 cut(s) | 167, 989, 2156 |
| Bst4CI | ACNGT | 7 cut(s) | 1231, 1241, 1467, 1715, 1895, 2134, 2392 |
| Bst6I | CTCTTC | 4 cut(s) | 84, 122, 440, 1898 |
| BstACI | GRCGYC | 1 cut(s) | 2047 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1852 |
| BstBAI | YACGTR | 1 cut(s) | 1760 |
| BstC8I | GCNNGC | 6 cut(s) | 172, 383, 454, 824, 1642, 2121 |
| BstDEI | CTNAG | 4 cut(s) | 967, 1274, 1875, 2061 |
| BstDSI | CCRYGG | 2 cut(s) | 278, 2133 |
| BstEII | GGTNACC | 1 cut(s) | 1772 |
| BstF5I | GGATG | 9 cut(s) | 105, 162, 257, 375, 805, 1106, 1591, 2168, 2287 |
| BstFNI | CGCG | 2 cut(s) | 498, 1644 |
| BstH2I | RGCGCY | 1 cut(s) | 1211 |
| BstHHI | GCGC | 3 cut(s) | 668, 1210, 1846 |
| BstMAI | GTCTC | 7 cut(s) | 530, 541, 632, 988, 1128, 1164, 1704 |
| BstMCI | CGRYCG | 4 cut(s) | 153, 684, 1046, 2303 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 192, 488, 1852, 2165 |
| BstNI | CCWGG | 3 cut(s) | 167, 989, 2156 |
| BstNSI | RCATGY | 1 cut(s) | 1153 |
| BstPI | GGTNACC | 1 cut(s) | 1772 |
| BstSCI | CCNGG | 7 cut(s) | 165, 361, 408, 987, 1446, 1447, 2154 |
| BstSFI | CTRYAG | 2 cut(s) | 1074, 1211 |
| BstSLI | GKGCMC | 2 cut(s) | 1642, 1883 |
| BstUI | CGCG | 2 cut(s) | 498, 1644 |
| BstV1I | GCAGC | 1 cut(s) | 1217 |
| BstV2I | GAAGAC | 1 cut(s) | 2351 |
| BstX2I | RGATCY | 1 cut(s) | 970 |
| BstYI | RGATCY | 1 cut(s) | 970 |
| BstZI | CGGCCG | 1 cut(s) | 2300 |
| Bsu15I | ATCGAT | 1 cut(s) | 2508 |
| BsuI | GTATCC | 1 cut(s) | 1703 |
| BsuTUI | ATCGAT | 1 cut(s) | 2508 |
| BtgI | CCRYGG | 2 cut(s) | 278, 2133 |
| BtgZI | GCGATG | 1 cut(s) | 2248 |
| BtsCI | GGATG | 9 cut(s) | 105, 162, 257, 375, 805, 1106, 1591, 2168, 2287 |
| BtsI | GCAGTG | 2 cut(s) | 32, 2241 |
| BtsIMutI | CAGTG | 7 cut(s) | 32, 506, 621, 1246, 1282, 1788, 2241 |
| Cac8I | GCNNGC | 6 cut(s) | 172, 383, 454, 824, 1642, 2121 |
| CfoI | GCGC | 3 cut(s) | 668, 1210, 1846 |
| Cfr10I | RCCGGY | 3 cut(s) | 125, 452, 662 |
| Cfr13I | GGNCC | 6 cut(s) | 734, 777, 1301, 1638, 1639, 2166 |
| Cfr9I | CCCGGG | 1 cut(s) | 1447 |
| ClaI | ATCGAT | 1 cut(s) | 2508 |
| CseI | GACGC | 1 cut(s) | 2356 |
| CsiI | ACCWGGT | 1 cut(s) | 987 |
| Csp6I | GTAC | 7 cut(s) | 583, 712, 766, 1364, 1567, 1872, 2077 |
| CviQI | GTAC | 7 cut(s) | 583, 712, 766, 1364, 1567, 1872, 2077 |
| DdeI | CTNAG | 4 cut(s) | 967, 1274, 1875, 2061 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2065 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2065 |
| EaeI | YGGCCR | 6 cut(s) | 184, 364, 827, 1062, 1558, 2300 |
| EagI | CGGCCG | 1 cut(s) | 2300 |
| Eam1104I | CTCTTC | 4 cut(s) | 84, 122, 440, 1898 |
| EarI | CTCTTC | 4 cut(s) | 84, 122, 440, 1898 |
| EciI | GGCGGA | 2 cut(s) | 368, 744 |
| Ecl136II | GAGCTC | 2 cut(s) | 195, 850 |
| EclXI | CGGCCG | 1 cut(s) | 2300 |
| Eco130I | CCWWGG | 1 cut(s) | 2473 |
| Eco24I | GRGCYC | 4 cut(s) | 35, 197, 852, 1642 |
| Eco31I | GGTCTC | 1 cut(s) | 632 |
| Eco32I | GATATC | 2 cut(s) | 226, 2024 |
| Eco47I | GGWCC | 2 cut(s) | 777, 1301 |
| Eco47III | AGCGCT | 1 cut(s) | 1209 |
| Eco52I | CGGCCG | 1 cut(s) | 2300 |
| Eco53kI | GAGCTC | 2 cut(s) | 195, 850 |
| Eco72I | CACGTG | 1 cut(s) | 1760 |
| Eco88I | CYCGRG | 2 cut(s) | 551, 1447 |
| Eco91I | GGTNACC | 1 cut(s) | 1772 |
| EcoICRI | GAGCTC | 2 cut(s) | 195, 850 |
| EcoO109I | RGGNCCY | 2 cut(s) | 734, 1638 |
| EcoO65I | GGTNACC | 1 cut(s) | 1772 |
| EcoRI | GAATTC | 1 cut(s) | 518 |
| EcoRII | CCWGG | 3 cut(s) | 165, 987, 2154 |
| EcoRV | GATATC | 2 cut(s) | 226, 2024 |
| EcoT14I | CCWWGG | 1 cut(s) | 2473 |
| EcoT38I | GRGCYC | 4 cut(s) | 35, 197, 852, 1642 |
| ErhI | CCWWGG | 1 cut(s) | 2473 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1563, 1595 |
| FaqI | GGGAC | 4 cut(s) | 487, 1453, 1777, 2470 |
| FauI | CCCGC | 2 cut(s) | 503, 1649 |
| FbaI | TGATCA | 1 cut(s) | 2197 |
| FblI | GTMKAC | 2 cut(s) | 153, 657 |
| Fnu4HI | GCNGC | 5 cut(s) | 450, 827, 1206, 1509, 1842 |
| FokI | GGATG | 9 cut(s) | 92, 149, 244, 362, 812, 1113, 1598, 2175, 2294 |
| FriOI | GRGCYC | 4 cut(s) | 35, 197, 852, 1642 |
| Fsp4HI | GCNGC | 5 cut(s) | 450, 827, 1206, 1509, 1842 |
| FspBI | CTAG | 4 cut(s) | 1196, 1437, 2112, 2439 |
| GlaI | GCGC | 3 cut(s) | 667, 1209, 1845 |
| GluI | GCNGC | 5 cut(s) | 450, 827, 1206, 1509, 1842 |
| GsuI | CTGGAG | 2 cut(s) | 409, 722 |
| HaeII | RGCGCY | 1 cut(s) | 1211 |
| HgaI | GACGC | 1 cut(s) | 2356 |
| HhaI | GCGC | 3 cut(s) | 668, 1210, 1846 |
| Hin1I | GRCGYC | 1 cut(s) | 2047 |
| Hin6I | GCGC | 3 cut(s) | 666, 1208, 1844 |
| HinP1I | GCGC | 3 cut(s) | 666, 1208, 1844 |
| HincII | GTYRAC | 5 cut(s) | 154, 658, 1186, 2051, 2481 |
| HindII | GTYRAC | 5 cut(s) | 154, 658, 1186, 2051, 2481 |
| HpaI | GTTAAC | 1 cut(s) | 1186 |
| Hpy188I | TCNGA | 8 cut(s) | 21, 515, 681, 1100, 1951, 2071, 2500, 2514 |
| Hpy99I | CGWCG | 5 cut(s) | 125, 491, 728, 788, 2049 |
| HpyCH4III | ACNGT | 7 cut(s) | 1231, 1241, 1467, 1715, 1895, 2134, 2392 |
| HpyCH4IV | ACGT | 4 cut(s) | 723, 1140, 1759, 2047 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 192, 488, 1852, 2165 |
| HpyF3I | CTNAG | 4 cut(s) | 967, 1274, 1875, 2061 |
| HpySE526I | ACGT | 4 cut(s) | 723, 1140, 1759, 2047 |
| Hsp92I | GRCGYC | 1 cut(s) | 2047 |
| HspAI | GCGC | 3 cut(s) | 666, 1208, 1844 |
| Kpn2I | TCCGGA | 1 cut(s) | 1370 |
| KroI | GCCGGC | 1 cut(s) | 452 |
| KroNI | GCCGGC | 1 cut(s) | 454 |
| Ksp22I | TGATCA | 1 cut(s) | 2197 |
| KspAI | GTTAAC | 1 cut(s) | 1186 |
| LguI | GCTCTTC | 1 cut(s) | 1898 |
| LmnI | GCTCC | 5 cut(s) | 38, 200, 428, 434, 1109 |
| Lsp1109I | GCAGC | 1 cut(s) | 1217 |
| LweI | GCATC | 6 cut(s) | 262, 266, 568, 1545, 1656, 2231 |
| MabI | ACCWGGT | 1 cut(s) | 987 |
| MaeI | CTAG | 4 cut(s) | 1196, 1437, 2112, 2439 |
| MaeII | ACGT | 4 cut(s) | 723, 1140, 1759, 2047 |
| MaeIII | GTNAC | 7 cut(s) | 251, 442, 1277, 1629, 1682, 1772, 2125 |
| MbiI | CCGCTC | 1 cut(s) | 826 |
| MboII | GAAGA | 9 cut(s) | 71, 109, 427, 1189, 1373, 1915, 1978, 2356, 2485 |
| MfeI | CAATTG | 2 cut(s) | 246, 2054 |
| MflI | RGATCY | 1 cut(s) | 970 |
| MhlI | GDGCHC | 6 cut(s) | 35, 197, 852, 1642, 1883, 1910 |
| MlsI | TGGCCA | 2 cut(s) | 1064, 1560 |
| MluNI | TGGCCA | 2 cut(s) | 1064, 1560 |
| MlyI | GAGTC | 8 cut(s) | 224, 509, 543, 990, 1274, 1383, 1442, 1759 |
| MmeI | TCCRAC | 3 cut(s) | 77, 442, 1528 |
| Mox20I | TGGCCA | 2 cut(s) | 1064, 1560 |
| MroI | TCCGGA | 1 cut(s) | 1370 |
| MroNI | GCCGGC | 1 cut(s) | 452 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1575 |
| MscI | TGGCCA | 2 cut(s) | 1064, 1560 |
| MseI | TTAA | 1 cut(s) | 1185 |
| MslI | CAYNNNNRTG | 8 cut(s) | 761, 1005, 1154, 1896, 2003, 2184, 2267, 2384 |
| Msp20I | TGGCCA | 2 cut(s) | 1064, 1560 |
| MspR9I | CCNGG | 7 cut(s) | 167, 363, 410, 989, 1448, 1449, 2156 |
| MunI | CAATTG | 2 cut(s) | 246, 2054 |
| MvaI | CCWGG | 3 cut(s) | 167, 989, 2156 |
| MvnI | CGCG | 2 cut(s) | 498, 1644 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 192, 488, 1852, 2165 |
| NaeI | GCCGGC | 1 cut(s) | 454 |
| NciI | CCSGG | 4 cut(s) | 363, 410, 1448, 1449 |
| NgoMIV | GCCGGC | 1 cut(s) | 452 |
| NlaIV | GGNNCC | 6 cut(s) | 34, 736, 1303, 1639, 1640, 1882 |
| NmeAIII | GCCGAG | 1 cut(s) | 298 |
| NmuCI | GTSAC | 6 cut(s) | 442, 1277, 1629, 1682, 1772, 2125 |
| NspI | RCATGY | 1 cut(s) | 1153 |
| OliI | CACNNNNGTG | 1 cut(s) | 2003 |
| PaeR7I | CTCGAG | 1 cut(s) | 551 |
| PciSI | GCTCTTC | 1 cut(s) | 1898 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 495 |
| PdiI | GCCGGC | 1 cut(s) | 454 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1575 |
| PfeI | GAWTC | 7 cut(s) | 600, 717, 1016, 1291, 1313, 1459, 2465 |
| Pfl23II | CGTACG | 1 cut(s) | 582 |
| PflFI | GACNNNGTC | 1 cut(s) | 1680 |
| PkrI | GCNGC | 5 cut(s) | 451, 828, 1207, 1510, 1843 |
| Ple19I | CGATCG | 1 cut(s) | 684 |
| PleI | GAGTC | 8 cut(s) | 224, 508, 543, 990, 1274, 1382, 1441, 1759 |
| PmaCI | CACGTG | 1 cut(s) | 1760 |
| PmlI | CACGTG | 1 cut(s) | 1760 |
| PpsI | GAGTC | 8 cut(s) | 224, 508, 543, 990, 1274, 1382, 1441, 1759 |
| Ppu21I | YACGTR | 1 cut(s) | 1760 |
| Psp124BI | GAGCTC | 2 cut(s) | 197, 852 |
| Psp6I | CCWGG | 3 cut(s) | 165, 987, 2154 |
| PspCI | CACGTG | 1 cut(s) | 1760 |
| PspEI | GGTNACC | 1 cut(s) | 1772 |
| PspGI | CCWGG | 3 cut(s) | 165, 987, 2154 |
| PspLI | CGTACG | 1 cut(s) | 582 |
| PspN4I | GGNNCC | 6 cut(s) | 34, 736, 1303, 1639, 1640, 1882 |
| PspOMI | GGGCCC | 1 cut(s) | 1638 |
| PspPI | GGNCC | 6 cut(s) | 734, 777, 1301, 1638, 1639, 2166 |
| PstI | CTGCAG | 1 cut(s) | 1078 |
| PsuI | RGATCY | 1 cut(s) | 970 |
| PsyI | GACNNNGTC | 1 cut(s) | 1680 |
| PvuI | CGATCG | 1 cut(s) | 684 |
| RsaI | GTAC | 7 cut(s) | 584, 713, 767, 1365, 1568, 1873, 2078 |
| RsaNI | GTAC | 7 cut(s) | 583, 712, 766, 1364, 1567, 1872, 2077 |
| RseI | CAYNNNNRTG | 8 cut(s) | 761, 1005, 1154, 1896, 2003, 2184, 2267, 2384 |
| SacI | GAGCTC | 2 cut(s) | 197, 852 |
| SalI | GTCGAC | 2 cut(s) | 152, 656 |
| SapI | GCTCTTC | 1 cut(s) | 1898 |
| SaqAI | TTAA | 1 cut(s) | 1185 |
| SatI | GCNGC | 5 cut(s) | 450, 827, 1206, 1509, 1842 |
| Sau96I | GGNCC | 6 cut(s) | 734, 777, 1301, 1638, 1639, 2166 |
| SchI | GAGTC | 8 cut(s) | 224, 509, 543, 990, 1274, 1383, 1442, 1759 |
| ScrFI | CCNGG | 7 cut(s) | 167, 363, 410, 989, 1448, 1449, 2156 |
| SduI | GDGCHC | 6 cut(s) | 35, 197, 852, 1642, 1883, 1910 |
| SexAI | ACCWGGT | 1 cut(s) | 987 |
| SfaNI | GCATC | 6 cut(s) | 262, 266, 568, 1545, 1656, 2231 |
| SfcI | CTRYAG | 2 cut(s) | 1074, 1211 |
| Sfr274I | CTCGAG | 1 cut(s) | 551 |
| SgrAI | CRCCGGYG | 1 cut(s) | 662 |
| SinI | GGWCC | 2 cut(s) | 777, 1301 |
| SlaI | CTCGAG | 1 cut(s) | 551 |
| SmaI | CCCGGG | 1 cut(s) | 1449 |
| SmiMI | CAYNNNNRTG | 8 cut(s) | 761, 1005, 1154, 1896, 2003, 2184, 2267, 2384 |
| SmlI | CTYRAG | 2 cut(s) | 551, 1580 |
| SmoI | CTYRAG | 2 cut(s) | 551, 1580 |
| SspI | AATATT | 1 cut(s) | 1266 |
| SspMI | CTAG | 4 cut(s) | 1196, 1437, 2112, 2439 |
| SstI | GAGCTC | 2 cut(s) | 197, 852 |
| StyD4I | CCNGG | 7 cut(s) | 165, 361, 408, 987, 1446, 1447, 2154 |
| StyI | CCWWGG | 1 cut(s) | 2473 |
| TaaI | ACNGT | 7 cut(s) | 1231, 1241, 1467, 1715, 1895, 2134, 2392 |
| TaiI | ACGT | 4 cut(s) | 726, 1143, 1762, 2050 |
| TaqII | GACCGA | 2 cut(s) | 897, 1032 |
| TatI | WGTACW | 2 cut(s) | 1363, 2076 |
| TauI | GCSGC | 4 cut(s) | 452, 829, 1511, 1844 |
| TfiI | GAWTC | 7 cut(s) | 600, 717, 1016, 1291, 1313, 1459, 2465 |
| Tru1I | TTAA | 1 cut(s) | 1185 |
| Tru9I | TTAA | 1 cut(s) | 1185 |
| TscAI | CASTG | 7 cut(s) | 32, 513, 628, 1246, 1282, 1795, 2241 |
| TseFI | GTSAC | 6 cut(s) | 442, 1277, 1629, 1682, 1772, 2125 |
| TseI | GCWGC | 1 cut(s) | 1205 |
| Tsp45I | GTSAC | 6 cut(s) | 442, 1277, 1629, 1682, 1772, 2125 |
| TspDTI | ATGAA | 8 cut(s) | 77, 364, 580, 816, 1737, 1814, 2439, 2478 |
| TspGWI | ACGGA | 5 cut(s) | 430, 475, 612, 1670, 2458 |
| TspMI | CCCGGG | 1 cut(s) | 1447 |
| TspRI | CASTG | 7 cut(s) | 32, 513, 628, 1246, 1282, 1795, 2241 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1680 |
| VpaK11BI | GGWCC | 2 cut(s) | 777, 1301 |
| XapI | RAATTY | 9 cut(s) | 178, 236, 518, 649, 788, 977, 1398, 2215, 2252 |
| XbaI | TCTAGA | 2 cut(s) | 1195, 2111 |
| XceI | RCATGY | 1 cut(s) | 1153 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1806 |
| XhoI | CTCGAG | 1 cut(s) | 551 |
| XmaI | CCCGGG | 1 cut(s) | 1447 |
| XmiI | GTMKAC | 2 cut(s) | 153, 657 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1575 |
| XspI | CTAG | 4 cut(s) | 1196, 1437, 2112, 2439 |
| ZraI | GACGTC | 1 cut(s) | 2048 |