Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0000121

Physical Location & Seq

Genomic Coordinates Forward (+)

76174792 .. 76177452

Gene Structure 2661 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000021523

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2661 bp

ATGAAACCCATTCTTGCTCCTCTTTACCCTTCCAGCTTGTTTCTACTCGTCGTCGCCCTACTGGTGGCCGGAGAGTCCACAACCATCTACACTCCGACCGATGATATCAACCTGAATTGTGGCTCATCTGGCGACCTGGTCTATGAATCTCAAACTTGGAGTGGAGATATCAACTCAAAGCTTTCCCCTATAGAAGTTGGTAGCACATCCCAAGTTTTGGAAGCACCACAGTCGTCCTCCACGGGCAGCCAAGTACCGTACACCACAGCACGGCTTTCTCGCTCGGAATTCACTTACCGATTTCCCGTCAGTCCGGGCCAGAAGTTCATCCGCTTATACTTCTACCCAGGCTCATACAACGCTGACTTCGACCGCTCCAAGTCGCTCTTCTCAGTCAAAGCCGGTGGCTATAACTACAGCCTTCTCAAAGACTTCAACGCATCTGTCAGTGCAGATGCCGATTCTCTTGAGACGATGCTTAAAGAATTTTGTATTAACATTGAGCCAGAACAACAGATTCTGAGCATCACATTCACTCCCAGCAAAGATGGATACGCGTTTATCAACGGGATTGAAATTATGTCCATGCCTGCCTATCTTTACTACACTGCCGCCCAAGACGATGGGCCTAGTATTATAGGCACCGAAAACCGAAACCCAGTGAAAAACAACATCGCTATGGAGATGATCTATCGAATCAACGTCGGCGGAAGTCCAGTCTCACCGAATGAAGACACGGGCATGTACCGCACCTGGGATTCAACGGACGAGATCTACTTAAATATTCAAAGTAGAACTTTGAGCTTGTTATCACAAAGCAATAATACTCATCTCAAATTTACAAAAATCCCGAACTACACTGCGCCGGTAGACGTGTACCGAACCGGCCGGTCAATGGAGGTGAACAGAACCATCAGCAAGAGCTACAATCTCACCTGGGAGTTTCCTGTAGATCCACAATTTTCTTACCTGGTGAGGCTCCATTTTTGTGAGATTATATTTAGATTGCAGAGAGATCGTGTGTTTCAGATTTTTATAGCCGACCAACCAGCTGAACAGAGAGCAGACATAATCCGATGGAGTGGAGGAAATGGGATTCCCGTATATAGAGACTACCTTGTGTTGGTCCCCAGTCTTCCAAACAAGAAGAAAGTTAATATGTCGGTTGTTCTGCAAGCAAATCAAGACGATTCGGAAACTCGATACAACGATGCACTCTTGAATGGGCTCGAAATCTTCAAACTGAATGACACAAACGGCAATCTCGCCGGACCCAATCCCGACCCACCACCGCCGAAGGAGAGACAAAATGTGAACCAGGACTTCCCTAGAAAATCGAAAAAGAAGTCGATTCCTATGCTTGCTATCCTCGCCGGTGTAGTTTCGGTCTTTCTTGTTCTTTCCGTCGCCGGTTTTTTACTTTTCAGGCGTGGAAGAAAAGTCAAGGACTCCTACTCCAGCAAAGCAACAAAGTGGGGGCCATTTTCATTTTCGACGACCAAGTCAACCAAGTCTCGCGGCTCATCTCTACCGTCGGACCTGTGTCGTCACTTTTCACTGGCTGAGATCAAGGCCGCCACTCAAAACTTCAACGATGTTTTCATCATCGGGGCTGGTGGGTTTGGTAACGTGTACAAAGGGTATATGGATGACGGGTCCACAGCCGTGGCGATCAAACGGCTGAAATCCGAGTCATCACAGGGAGCCCACGAGTTCAAGACCGAGATAGAGATGCTCTCCCAACTCCGCCACCTCCATTTGGTGTCTCTCGTTGGATATTGCACTGAGCAAGGTGAGATGATCTTGGTGTATGACTACATGGCACGTGGGACTCTCCGAGACCATTTGTACAAAACCGAAAATCCACCACTTCCGTGGGAACAACGGCTCCAAATTTGTATTGGTGCGGCGCGTGGGTTGCACTACCTTCACACGGGGGCCATGCGCATGATTATCCACCGCGACGTGAAGAGCACAAATATCTTATTGGATGAGAAATGGGTGGCCAAGGTTTCGGACTTTGGGTTGTCCAAAATGGGCACCACCACTTTGTCCAAGCCTCACATCAGTACAGTGGTAAAGGGTAGTTTTGGGTATCTCGACCCGGAGTACTATAGACGTCAACAGTTGACTGAGAAATCGGATGTCTACTCTTTTGGAGTAGTCTTGTGTGAGGTTTTGTGTGCGAGGCCAGCTCTGATACGTACGGCGGAGAAGAAGCAAATGAGCTTAGCTGAGTGGACCAAGACTAGTTATCGAAATGGCGCGATTGATCAAATCATTGATCCCTATCTGAGGGGAAAGATTGCAGCCACATGTCTGAATAAGTTCGTTGAGCTTGCGTTGAGTTGTATGAATGATAATGGAAGTGAGCGGCCATCGATGAATGACGTAGTATGGGGGCTTGAGTTTGCACTGCAGCTTCAGCAGAGTGCGGAAGCAATGTTATACTCCAACGTCGAAAAGGAAGGTGAAGATGAGGTTGCATTCATGAAAGGTAATGATGCTGGGATGAGTTGTAGCTGGGAATACTCGTCGGATTCGAAGAACAGCAGAACGACCAAAACTAGTAGCGATGAGCAAAGTTCTACCACCAATGAATCCATGAAAGGCATGTCTGGAACAATCTTCTCTGAGATCAAGGATCCCACTGTGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009723 GO:0009725 GO:0009741 GO:0009787 GO:0009788 GO:0009791 GO:0009856 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010118 GO:0010483 GO:0010646 GO:0010648 GO:0014070 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022622 GO:0023051 GO:0023057 GO:0030054 GO:0030308 GO:0030312 GO:0032501 GO:0032502 GO:0033993 GO:0036211 GO:0040008 GO:0042221 GO:0043170 GO:0043207 GO:0043412 GO:0043680 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044426 GO:0044462 GO:0044464 GO:0044703 GO:0044706 GO:0045926 GO:0046777 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051128 GO:0051703 GO:0051704 GO:0051707 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:0098542 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1905957 GO:1905958

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Malectin_like Pkinase_Tyr

Protein Analysis

887

Amino Acids

99.07

Weight (kDa)

6.85

Isoelectric Point (pI)

44.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Malectin_like	PF12819	38 - 414	3.7e-31	Malectin-like domain
Malectin	PF11721	230 - 372	4.1e-10	Malectin domain
Pkinase	PF00069	534 - 798	1.6e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	536 - 799	5.2e-49	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000121)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g46570 FvH4_6g46580 FvH4_6g46610 FvH4_6g46620 FvH4_6g46640 FvH4_6g46680
malus_domestica	MD03G1063600.v1.1 MD03G1063700.v1.1 MD09G1069300.v1.1 MD09G1069400.v1.1 MD11G1067200.v1.1 MD11G1070300.v1.1 MD11G1153300.v1.1 MD17G1060300.v1.1 MD17G1061900.v1.1 MD17G1062100.v1.1 MD17G1062300.v1.1 MD17G1062400.v1.1 MD17G1063000.v1.1 MD17G1063200.v1.1 MD17G1063600.v1.1
prunus_persica	Prupe.3G246200_v2.0.a1 Prupe.3G251000_v2.0.a1 Prupe.3G251100_v2.0.a1 Prupe.3G251200_v2.0.a1 Prupe.3G251600_v2.0.a1 Prupe.3G251700_v2.0.a1 Prupe.3G251800_v2.0.a1 Prupe.3G251900_v2.0.a1
pyrus_communis	pycom03g05070 pycom03g05100 pycom12433g00070 pycom17g06000 pycom17g06170 pycom17g06180 pycom17g06200 pycom17g06210 pycom17g06280 pycom17g06300 pycom17g06310 pycom17g06320 pycom17g06330 pycom17g06350 pycom17g06370 pycom17g06380 pycom17g06400
rosa_chinensis	RchiOBHm_Chr1g0321851 RchiOBHm_Chr1g0321911 RchiOBHm_Chr1g0323951 RchiOBHm_Chr1g0361941 RchiOBHm_Chr2g0164711 RchiOBHm_Chr2g0165301 RchiOBHm_Chr2g0165311 RchiOBHm_Chr2g0165321 RchiOBHm_Chr2g0165351 RchiOBHm_Chr2g0165431 RchiOBHm_Chr2g0165461 RchiOBHm_Chr2g0165491 RchiOBHm_Chr2g0165511 RchiOBHm_Chr2g0165521 RchiOBHm_Chr2g0165531 RchiOBHm_Chr3g0481631 RchiOBHm_Chr3g0481801 RchiOBHm_Chr3g0481811 RchiOBHm_Chr3g0481821 RchiOBHm_Chr3g0481831 RchiOBHm_Chr4g0400291 RchiOBHm_Chr4g0400371 RchiOBHm_Chr4g0400391 RchiOBHm_Chr6g0274711 RchiOBHm_Chr6g0274721 RchiOBHm_Chr7g0211011
rosa_laevigata	RLG00000002260 RLG00000002262 RLG00000002263 RLG00000003027 RLG00000003028 RLG00000009196 RLG00000009197 RLG00000012573 RLG00000020400 RLG00000021523 RLG00000021524 RLG00000021525 RLG00000021527 RLG00000021528 RLG00000021529 RLG00000021530 RLG00000021532 RLG00000021533 RLG00000021534 RLG00000021535 RLG00000021537 RLG00000023384 RLG00000027681 RLG00000027682 RLG00000027685 RLG00000030240 RLG00000030401
rosa_multiflora	Rmu_co7998078.1_g000001 Rmu_co8009032.1_g000001 Rmu_co8326071.1_g000001 Rmu_co8333709.1_g000001 Rmu_co8362007.1_g000001 Rmu_co8369261.1_g000001 Rmu_co8391219.1_g000001 Rmu_co8456951.1_g000001 Rmu_co8461523.1_g000001 Rmu_sc0000427.1_g000009 Rmu_sc0000427.1_g000032 Rmu_sc0000427.1_g000033 Rmu_sc0000521.1_g000005 Rmu_sc0000521.1_g000008 Rmu_sc0000521.1_g000009 Rmu_sc0000655.1_g000016 Rmu_sc0000655.1_g000017 Rmu_sc0000655.1_g000018 Rmu_sc0000675.1_g000003 Rmu_sc0000795.1_g000018 Rmu_sc0000795.1_g000083 Rmu_sc0001306.1_g000042 Rmu_sc0001306.1_g000043 Rmu_sc0001873.1_g000001 Rmu_sc0001873.1_g000010 Rmu_sc0002529.1_g000002 Rmu_sc0002529.1_g000004 Rmu_sc0003410.1_g000004 Rmu_sc0003909.1_g000010 Rmu_sc0004097.1_g000001 Rmu_sc0005425.1_g000003 Rmu_sc0005425.1_g000004 Rmu_sc0005425.1_g000010 Rmu_sc0005425.1_g000012 Rmu_sc0005425.1_g000017 Rmu_sc0006223.1_g000014 Rmu_sc0006399.1_g000007 Rmu_sc0010324.1_g000001 Rmu_sc0010409.1_g000005 Rmu_sc0013532.1_g000005 Rmu_sc0016601.1_g000002 Rmu_sc0016601.1_g000003 Rmu_sc0016601.1_g000006 Rmu_sc0017773.1_g000001 Rmu_sc0017773.1_g000002 Rmu_sc0019514.1_g000002 Rmu_sc0021575.1_g000001 Rmu_sc0023720.1_g000002 Rmu_sc0030898.1_g000001 Rmu_sc0033982.1_g000001 Rmu_sc0034651.1_g000001 Rmu_sc0038482.1_g000001 Rmu_sc0038644.1_g000001 Rmu_sc0038644.1_g000003 Rmu_sc0039243.1_g000001 Rmu_sc0042715.1_g000001 Rmu_ssc0000124.1_g000003
rosa_roxburghii	Rroxscaffold_2G00085740 Rroxscaffold_2G00085760 Rroxscaffold_2G00085780 Rroxscaffold_2G00085800 Rroxscaffold_2G00085830 Rroxscaffold_2G00085850 Rroxscaffold_2G00085860 Rroxscaffold_4G00294610 Rroxscaffold_4G00326070 Rroxscaffold_4G00326080 Rroxscaffold_4G00327420 Rroxscaffold_4G00327430 Rroxscaffold_4G00327460 Rroxscaffold_4G00327470 Rroxscaffold_5G00345350 Rroxscaffold_6G00399890 Rroxscaffold_6G00400250 Rroxscaffold_7G00194000 Rroxscaffold_7G00194020 Rroxscaffold_7G00194050
rosa_rugosa	Rorug01G0034300 Rorug01G0034500 Rorug01G0040700 Rorug01G0064300 Rorug02G0513700 Rorug02G0513800 Rorug02G0513900 Rorug02G0514000 Rorug02G0514000 Rorug02G0514100 Rorug02G0514300 Rorug02G0514400 Rorug02G0514700 Rorug02G0515000 Rorug02G0515100 Rorug02G0515300 Rorug02G0515500 Rorug02G0515600 Rorug02G0515800 Rorug02G0515900 Rorug02G0516000 Rorug03G0193200 Rorug04G0024600 Rorug06G0083100 Rorug06G0085200 Rorug07G0188600
rosa_samantha	Rh1CG234000 Rh2DG603700 Rh2DG603800 Rh2DG603900 Rh6BG203000
rosa_wichuraiana	Rw0G010290 Rw1G004240 Rw1G005000 Rw1G026960 Rw2G048310 Rw2G048320 Rw2G048330 Rw2G048350 Rw2G048360 Rw2G048380 Rw2G048430 Rw2G048460 Rw2G048500 Rw2G048510 Rw2G048520 Rw2G048530 Rw3G021810 Rw4G008270 Rw6G017320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1501
AatII	GACGTC	1 cut(s)	2122
Acc16I	TGCGCA	1 cut(s)	1946
AccB1I	GGYRCC	2 cut(s)	641, 2039
AccB7I	CCANNNNNTGG	1 cut(s)	217
AccBSI	CCGCTC	2 cut(s)	375, 2374
AccI	GTMKAC	2 cut(s)	870, 2148
AccII	CGCG	5 cut(s)	557, 1518, 1912, 1962, 2267
AclWI	GGATC	4 cut(s)	947, 2279, 2639, 2652
AcoI	YGGCCR	4 cut(s)	66, 886, 2004, 2375
AcsI	RAATTY	4 cut(s)	287, 485, 836, 1893
AcuI	CTGAAG	1 cut(s)	2408
AcvI	CACGTG	1 cut(s)	1826
AcyI	GRCGYC	1 cut(s)	2119
AfaI	GTAC	9 cut(s)	255, 260, 746, 878, 1634, 1850, 2071, 2111, 2206
AfiI	CCNNNNNNNGG	9 cut(s)	64, 217, 270, 754, 895, 1123, 1759, 1933, 2295
AflIII	ACRYGT	4 cut(s)	555, 873, 1629, 2315
AgsI	TTSAA	8 cut(s)	436, 575, 762, 788, 1222, 1240, 1591, 1717
AhlI	ACTAGT	2 cut(s)	2249, 2567
AjiI	CACGTC	2 cut(s)	874, 1966
AjnI	CCWGG	6 cut(s)	135, 346, 752, 935, 969, 1317
AjuI	GAANNNNNNNTTGG	2 cut(s)	371, 403
AleI	CACNNNNGTG	2 cut(s)	1664, 1873
AloI	GAACNNNNNNTCC	2 cut(s)	1872, 1904
Alw21I	GWGCWC	1 cut(s)	1976
Alw26I	GTCTC	7 cut(s)	464, 724, 1104, 1297, 1518, 1770, 1833
AlwI	GGATC	4 cut(s)	947, 2279, 2639, 2652
AlwNI	CAGNNNCTG	1 cut(s)	520
ApeKI	GCWGC	3 cut(s)	246, 2309, 2419
ApoI	RAATTY	4 cut(s)	287, 485, 836, 1893
ArsI	GACNNNNNNTTYG	2 cut(s)	1331, 1363
Asp700I	GAANNNNTTC	1 cut(s)	2327
AspLEI	GCGC	4 cut(s)	865, 1912, 1947, 2267
AspS9I	GGNCC	9 cut(s)	316, 626, 1126, 1271, 1478, 1537, 1656, 1938, 2241
AsuC2I	CCSGG	2 cut(s)	315, 2105
AsuHPI	GGTGA	6 cut(s)	714, 913, 925, 985, 1805, 2483
AsuII	TTCGAA	1 cut(s)	2543
AvaII	GGWCC	5 cut(s)	1126, 1271, 1537, 1656, 2241
BaeGI	GKGCMC	1 cut(s)	2042
BalI	TGGCCA	1 cut(s)	2006
BamHI	GGATCC	1 cut(s)	2644
BanI	GGYRCC	2 cut(s)	641, 2039
BanII	GRGCYC	2 cut(s)	1230, 1708
BauI	CACGAG	1 cut(s)	1709
BbrPI	CACGTG	1 cut(s)	1826
BbsI	GAAGAC	2 cut(s)	738, 1127
Bbv12I	GWGCWC	1 cut(s)	1976
BbvI	GCAGC	3 cut(s)	258, 2321, 2431
BccI	CCATC	6 cut(s)	92, 542, 617, 920, 1071, 2386
BceAI	ACGGC	6 cut(s)	287, 1273, 1649, 1694, 1901, 2223
BcgI	CGANNNNNNTGC	6 cut(s)	213, 247, 998, 1032, 1339, 1373
BciT130I	CCWGG	6 cut(s)	137, 348, 754, 937, 971, 1319
BciVI	GTATCC	1 cut(s)	545
BclI	TGATCA	1 cut(s)	2272
BcnI	CCSGG	2 cut(s)	315, 2105
BcoDI	GTCTC	7 cut(s)	464, 724, 1104, 1297, 1518, 1770, 1833
BcuI	ACTAGT	2 cut(s)	2249, 2567
BfaI	CTAG	4 cut(s)	630, 1329, 2250, 2568
BfmI	CTRYAG	5 cut(s)	189, 415, 948, 2113, 2417
BfuI	GTATCC	1 cut(s)	545
BglII	AGATCT	1 cut(s)	771
BisI	GCNGC	8 cut(s)	247, 612, 1519, 1575, 1908, 2310, 2375, 2420
BlpI	GCTNAGC	1 cut(s)	2230
BlsI	GCNGC	8 cut(s)	248, 613, 1520, 1576, 1909, 2311, 2376, 2421
BmcAI	AGTACT	1 cut(s)	2111
Bme1390I	CCNGG	8 cut(s)	137, 315, 348, 754, 937, 971, 1319, 2105
Bme18I	GGWCC	5 cut(s)	1126, 1271, 1537, 1656, 2241
BmgBI	CACGTC	2 cut(s)	874, 1966
BmgT120I	GGNCC	9 cut(s)	316, 626, 1126, 1271, 1478, 1537, 1656, 1938, 2241
BmrFI	CCNGG	8 cut(s)	137, 315, 348, 754, 937, 971, 1319, 2105
BmrI	ACTGGG	2 cut(s)	653, 1125
BmsI	GCATC	7 cut(s)	445, 449, 465, 534, 1201, 1722, 2494
BmuI	ACTGGG	2 cut(s)	653, 1125
BoxI	GACNNNNGTC	1 cut(s)	1542
BpiI	GAAGAC	2 cut(s)	738, 1127
BplI	GAGNNNNNCTC	4 cut(s)	1721, 1753, 2179, 2211
BpmI	CTGGAG	1 cut(s)	1441
Bpu1102I	GCTNAGC	1 cut(s)	2230
Bpu14I	TTCGAA	1 cut(s)	2543
BpuEI	CTTGAG	2 cut(s)	488, 2426
BpuMI	CCSGG	2 cut(s)	315, 2105
Bsa29I	ATCGAT	1 cut(s)	2381
BsaAI	YACGTR	2 cut(s)	1826, 2204
BsaBI	GATNNNNATC	1 cut(s)	2289
BsaHI	GRCGYC	1 cut(s)	2119
BsaI	GGTCTC	1 cut(s)	1833
BsaJI	CCNNGG	7 cut(s)	240, 346, 753, 936, 1665, 1874, 2007
BsaXI	ACNNNNNCTCC	6 cut(s)	520, 550, 1439, 1469, 1872, 1902
Bsc4I	CCNNNNNNNGG	9 cut(s)	64, 217, 270, 754, 895, 1123, 1759, 1933, 2295
Bse118I	RCCGGY	6 cut(s)	401, 865, 884, 888, 1373, 1409
Bse1I	ACTGG	5 cut(s)	66, 659, 716, 1131, 1563
Bse3DI	GCAATG	1 cut(s)	2448
Bse8I	GATNNNNATC	1 cut(s)	2289
BseBI	CCWGG	6 cut(s)	137, 348, 754, 937, 971, 1319
BseCI	ATCGAT	1 cut(s)	2381
BseDI	CCNNGG	7 cut(s)	240, 346, 753, 936, 1665, 1874, 2007
BseGI	GGATG	6 cut(s)	206, 327, 1654, 1996, 2149, 2517
BseJI	GATNNNNATC	1 cut(s)	2289
BseLI	CCNNNNNNNGG	9 cut(s)	64, 217, 270, 754, 895, 1123, 1759, 1933, 2295
BseMI	GCAATG	1 cut(s)	2448
BseMII	CTCAG	8 cut(s)	405, 512, 1554, 1776, 2124, 2226, 2285, 2625
BseNI	ACTGG	5 cut(s)	66, 659, 716, 1131, 1563
BseRI	GAGGAG	1 cut(s)	9
BseSI	GKGCMC	1 cut(s)	2042
BseX3I	CGGCCG	1 cut(s)	886
BseXI	GCAGC	3 cut(s)	258, 2321, 2431
BseYI	CCCAGC	3 cut(s)	539, 2507, 2523
BsgI	GTGCAG	1 cut(s)	471
Bsh1236I	CGCG	5 cut(s)	557, 1518, 1912, 1962, 2267
Bsh1285I	CGRYCG	3 cut(s)	99, 373, 889
BshNI	GGYRCC	2 cut(s)	641, 2039
BshVI	ATCGAT	1 cut(s)	2381
BsiEI	CGRYCG	3 cut(s)	99, 373, 889
BsiHKAI	GWGCWC	1 cut(s)	1976
BsiWI	CGTACG	1 cut(s)	2204
BslFI	GGGAC	2 cut(s)	1112, 1843
BslI	CCNNNNNNNGG	9 cut(s)	64, 217, 270, 754, 895, 1123, 1759, 1933, 2295
BsmAI	GTCTC	7 cut(s)	464, 724, 1104, 1297, 1518, 1770, 1833
BsmBI	CGTCTC	1 cut(s)	464
BsmFI	GGGAC	2 cut(s)	1112, 1843
BsmI	GAATGC	1 cut(s)	2486
Bso31I	GGTCTC	1 cut(s)	1833
Bsp119I	TTCGAA	1 cut(s)	2543
Bsp1286I	GDGCHC	4 cut(s)	1230, 1708, 1976, 2042
Bsp1407I	TGTACA	2 cut(s)	1632, 1848
Bsp1720I	GCTNAGC	1 cut(s)	2230
BspCNI	CTCAG	8 cut(s)	404, 513, 1555, 1777, 2125, 2227, 2286, 2626
BspDI	ATCGAT	1 cut(s)	2381
BspFNI	CGCG	5 cut(s)	557, 1518, 1912, 1962, 2267
BspHI	TCATGA	1 cut(s)	2490
BspMAI	CTGCAG	1 cut(s)	2421
BspPI	GGATC	4 cut(s)	947, 2279, 2639, 2652
BspQI	GCTCTTC	2 cut(s)	392, 1964
BspT104I	TTCGAA	1 cut(s)	2543
BspT107I	GGYRCC	2 cut(s)	641, 2039
BspTNI	GGTCTC	1 cut(s)	1833
BsrBI	CCGCTC	2 cut(s)	375, 2374
BsrDI	GCAATG	1 cut(s)	2448
BsrFI	RCCGGY	6 cut(s)	401, 865, 884, 888, 1373, 1409
BsrGI	TGTACA	2 cut(s)	1632, 1848
BsrI	ACTGG	5 cut(s)	66, 659, 716, 1131, 1563
BssAI	RCCGGY	6 cut(s)	401, 865, 884, 888, 1373, 1409
BssECI	CCNNGG	7 cut(s)	240, 346, 753, 936, 1665, 1874, 2007
BssNI	GRCGYC	1 cut(s)	2119
BssSI	CACGAG	1 cut(s)	1709
BssT1I	CCWWGG	1 cut(s)	2007
Bst2BI	CACGAG	1 cut(s)	1709
Bst2UI	CCWGG	6 cut(s)	137, 348, 754, 937, 971, 1319
Bst4CI	ACNGT	6 cut(s)	231, 258, 1533, 2074, 2127, 2653
Bst6I	CTCTTC	2 cut(s)	392, 1964
BstACI	GRCGYC	1 cut(s)	2119
BstAUI	TGTACA	2 cut(s)	1632, 1848
BstBAI	YACGTR	2 cut(s)	1826, 2204
BstBI	TTCGAA	1 cut(s)	2543
BstC8I	GCNNGC	5 cut(s)	591, 1176, 1362, 2193, 2340
BstDEI	CTNAG	9 cut(s)	391, 521, 1563, 1785, 2133, 2230, 2235, 2294, 2634
BstDSI	CCRYGG	3 cut(s)	240, 1665, 1874
BstENI	CCTNNNNNAGG	1 cut(s)	2293
BstF5I	GGATG	6 cut(s)	206, 327, 1654, 1996, 2149, 2517
BstFNI	CGCG	5 cut(s)	557, 1518, 1912, 1962, 2267
BstHHI	GCGC	4 cut(s)	865, 1912, 1947, 2267
BstMAI	GTCTC	7 cut(s)	464, 724, 1104, 1297, 1518, 1770, 1833
BstMCI	CGRYCG	3 cut(s)	99, 373, 889
BstMWI	GCNNNNNNNGC	6 cut(s)	129, 747, 1370, 1918, 2192, 2425
BstNI	CCWGG	6 cut(s)	137, 348, 754, 937, 971, 1319
BstNSI	RCATGY	3 cut(s)	745, 2319, 2617
BstPAI	GACNNNNGTC	1 cut(s)	1542
BstSCI	CCNGG	8 cut(s)	135, 313, 346, 752, 935, 969, 1317, 2103
BstSFI	CTRYAG	5 cut(s)	189, 415, 948, 2113, 2417
BstSLI	GKGCMC	1 cut(s)	2042
BstSNI	TACGTA	1 cut(s)	2204
BstUI	CGCG	5 cut(s)	557, 1518, 1912, 1962, 2267
BstV1I	GCAGC	3 cut(s)	258, 2321, 2431
BstV2I	GAAGAC	2 cut(s)	738, 1127
BstX2I	RGATCY	3 cut(s)	771, 952, 2644
BstXI	CCANNNNNNTGG	3 cut(s)	623, 1666, 1875
BstYI	RGATCY	3 cut(s)	771, 952, 2644
BstZI	CGGCCG	1 cut(s)	886
Bsu15I	ATCGAT	1 cut(s)	2381
BsuI	GTATCC	1 cut(s)	545
BsuTUI	ATCGAT	1 cut(s)	2381
BtgI	CCRYGG	3 cut(s)	240, 1665, 1874
BtgZI	GCGATG	2 cut(s)	658, 2589
BtrI	CACGTC	2 cut(s)	874, 1966
BtsCI	GGATG	6 cut(s)	206, 327, 1654, 1996, 2149, 2517
BtsI	GCAGTG	3 cut(s)	606, 858, 2414
BtsIMutI	CAGTG	9 cut(s)	454, 606, 666, 858, 1556, 1782, 2079, 2414, 2649
Cac8I	GCNNGC	5 cut(s)	591, 1176, 1362, 2193, 2340
CaiI	CAGNNNCTG	1 cut(s)	520
CciI	TCATGA	1 cut(s)	2490
CfoI	GCGC	4 cut(s)	865, 1912, 1947, 2267
Cfr10I	RCCGGY	6 cut(s)	401, 865, 884, 888, 1373, 1409
Cfr13I	GGNCC	9 cut(s)	316, 626, 1126, 1271, 1478, 1537, 1656, 1938, 2241
ClaI	ATCGAT	1 cut(s)	2381
CsiI	ACCWGGT	2 cut(s)	135, 969
Csp6I	GTAC	9 cut(s)	254, 259, 745, 877, 1633, 1849, 2070, 2110, 2205
CspCI	CAANNNNNGTGG	2 cut(s)	385, 420
CviAII	CATG	9 cut(s)	586, 742, 1819, 1942, 1948, 2316, 2491, 2605, 2614
CviQI	GTAC	9 cut(s)	254, 259, 745, 877, 1633, 1849, 2070, 2110, 2205
DdeI	CTNAG	9 cut(s)	391, 521, 1563, 1785, 2133, 2230, 2235, 2294, 2634
DrdI	GACNNNNNNGTC	1 cut(s)	1501
DseDI	GACNNNNNNGTC	1 cut(s)	1501
EaeI	YGGCCR	4 cut(s)	66, 886, 2004, 2375
EagI	CGGCCG	1 cut(s)	886
Eam1104I	CTCTTC	2 cut(s)	392, 1964
EarI	CTCTTC	2 cut(s)	392, 1964
EciI	GGCGGA	3 cut(s)	723, 1736, 2225
EclXI	CGGCCG	1 cut(s)	886
Eco105I	TACGTA	1 cut(s)	2204
Eco130I	CCWWGG	1 cut(s)	2007
Eco24I	GRGCYC	2 cut(s)	1230, 1708
Eco31I	GGTCTC	1 cut(s)	1833
Eco32I	GATATC	2 cut(s)	106, 169
Eco47I	GGWCC	5 cut(s)	1126, 1271, 1537, 1656, 2241
Eco52I	CGGCCG	1 cut(s)	886
Eco57I	CTGAAG	1 cut(s)	2408
Eco72I	CACGTG	1 cut(s)	1826
EcoNI	CCTNNNNNAGG	1 cut(s)	2293
EcoRI	GAATTC	1 cut(s)	287
EcoRII	CCWGG	6 cut(s)	135, 346, 752, 935, 969, 1317
EcoRV	GATATC	2 cut(s)	106, 169
EcoT14I	CCWWGG	1 cut(s)	2007
EcoT38I	GRGCYC	2 cut(s)	1230, 1708
ErhI	CCWWGG	1 cut(s)	2007
Esp3I	CGTCTC	1 cut(s)	464
FaeI	CATG	9 cut(s)	589, 745, 1822, 1945, 1951, 2319, 2494, 2608, 2617
FalI	AAGNNNNNCTT	4 cut(s)	371, 403, 781, 813
FaqI	GGGAC	2 cut(s)	1112, 1843
FatI	CATG	9 cut(s)	585, 741, 1818, 1941, 1947, 2315, 2490, 2604, 2613
FbaI	TGATCA	1 cut(s)	2272
FblI	GTMKAC	2 cut(s)	870, 2148
Fnu4HI	GCNGC	8 cut(s)	247, 612, 1519, 1575, 1908, 2310, 2375, 2420
FokI	GGATG	6 cut(s)	193, 314, 1661, 2003, 2156, 2524
FriOI	GRGCYC	2 cut(s)	1230, 1708
Fsp4HI	GCNGC	8 cut(s)	247, 612, 1519, 1575, 1908, 2310, 2375, 2420
FspAI	RTGCGCAY	1 cut(s)	1946
FspBI	CTAG	4 cut(s)	630, 1329, 2250, 2568
FspI	TGCGCA	1 cut(s)	1946
GlaI	GCGC	4 cut(s)	864, 1911, 1946, 2266
GluI	GCNGC	8 cut(s)	247, 612, 1519, 1575, 1908, 2310, 2375, 2420
GsaI	CCCAGC	3 cut(s)	543, 2511, 2527
GsuI	CTGGAG	1 cut(s)	1441
HhaI	GCGC	4 cut(s)	865, 1912, 1947, 2267
Hin1I	GRCGYC	1 cut(s)	2119
Hin1II	CATG	9 cut(s)	589, 745, 1822, 1945, 1951, 2319, 2494, 2608, 2617
Hin6I	GCGC	4 cut(s)	863, 1910, 1945, 2265
HinP1I	GCGC	4 cut(s)	863, 1910, 1945, 2265
HincII	GTYRAC	3 cut(s)	1506, 2123, 2130
HindII	GTYRAC	3 cut(s)	1506, 2123, 2130
HindIII	AAGCTT	1 cut(s)	179
HphI	GGTGA	6 cut(s)	714, 913, 925, 985, 1805, 2483
Hpy188III	TCNNGA	9 cut(s)	467, 850, 1184, 1219, 1280, 1717, 2099, 2491, 2619
Hpy99I	CGWCG	9 cut(s)	53, 56, 707, 1409, 1498, 1537, 1967, 2462, 2539
HpyAV	CCTTC	5 cut(s)	39, 431, 1291, 1937, 2462
HpyCH4III	ACNGT	6 cut(s)	231, 258, 1533, 2074, 2127, 2653
HpyCH4IV	ACGT	9 cut(s)	702, 873, 1629, 1825, 1965, 2119, 2203, 2391, 2457
HpyF10VI	GCNNNNNNNGC	6 cut(s)	129, 747, 1370, 1918, 2192, 2425
HpyF3I	CTNAG	9 cut(s)	391, 521, 1563, 1785, 2133, 2230, 2235, 2294, 2634
HpySE526I	ACGT	9 cut(s)	702, 873, 1629, 1825, 1965, 2119, 2203, 2391, 2457
Hsp92I	GRCGYC	1 cut(s)	2119
Hsp92II	CATG	9 cut(s)	589, 745, 1822, 1945, 1951, 2319, 2494, 2608, 2617
HspAI	GCGC	4 cut(s)	863, 1910, 1945, 2265
Ksp22I	TGATCA	1 cut(s)	2272
LguI	GCTCTTC	2 cut(s)	392, 1964
LmnI	GCTCC	5 cut(s)	22, 380, 984, 1703, 1893
Lsp1109I	GCAGC	3 cut(s)	258, 2321, 2431
LweI	GCATC	7 cut(s)	445, 449, 465, 534, 1201, 1722, 2494
MabI	ACCWGGT	2 cut(s)	135, 969
MaeI	CTAG	4 cut(s)	630, 1329, 2250, 2568
MaeII	ACGT	9 cut(s)	702, 873, 1629, 1825, 1965, 2119, 2203, 2391, 2457
MaeIII	GTNAC	2 cut(s)	1547, 1625
MbiI	CCGCTC	2 cut(s)	375, 2374
MflI	RGATCY	3 cut(s)	771, 952, 2644
MhlI	GDGCHC	4 cut(s)	1230, 1708, 1976, 2042
MlsI	TGGCCA	1 cut(s)	2006
MluCI	AATT	7 cut(s)	115, 287, 485, 576, 836, 959, 1893
MluI	ACGCGT	1 cut(s)	555
MluNI	TGGCCA	1 cut(s)	2006
MlyI	GAGTC	4 cut(s)	83, 1442, 1700, 1825
MmeI	TCCRAC	5 cut(s)	119, 1515, 1753, 2478, 2517
Mox20I	TGGCCA	1 cut(s)	2006
MroXI	GAANNNNTTC	1 cut(s)	2327
MscI	TGGCCA	1 cut(s)	2006
MseI	TTAA	4 cut(s)	480, 495, 779, 1155
MslI	CAYNNNNRTG	6 cut(s)	677, 740, 987, 1664, 1873, 1946
Msp20I	TGGCCA	1 cut(s)	2006
MspA1I	CMGCKG	1 cut(s)	1052
MspR9I	CCNGG	8 cut(s)	137, 315, 348, 754, 937, 971, 1319, 2105
Mva1269I	GAATGC	1 cut(s)	2486
MvaI	CCWGG	6 cut(s)	137, 348, 754, 937, 971, 1319
MvnI	CGCG	5 cut(s)	557, 1518, 1912, 1962, 2267
MwoI	GCNNNNNNNGC	6 cut(s)	129, 747, 1370, 1918, 2192, 2425
NciI	CCSGG	2 cut(s)	315, 2105
NlaIII	CATG	9 cut(s)	589, 745, 1822, 1945, 1951, 2319, 2494, 2608, 2617
NmuCI	GTSAC	1 cut(s)	1547
NsbI	TGCGCA	1 cut(s)	1946
NspI	RCATGY	3 cut(s)	745, 2319, 2617
NspV	TTCGAA	1 cut(s)	2543
OliI	CACNNNNGTG	2 cut(s)	1664, 1873
PagI	TCATGA	1 cut(s)	2490
PciI	ACATGT	1 cut(s)	2315
PciSI	GCTCTTC	2 cut(s)	392, 1964
PcsI	WCGNNNNNNNCGW	2 cut(s)	366, 2540
PctI	GAATGC	1 cut(s)	2486
PdmI	GAANNNNTTC	1 cut(s)	2327
Pfl23II	CGTACG	1 cut(s)	2204
PflFI	GACNNNGTC	2 cut(s)	137, 1501
PflMI	CCANNNNNTGG	1 cut(s)	217
PkrI	GCNGC	8 cut(s)	248, 613, 1520, 1576, 1909, 2311, 2376, 2421
PleI	GAGTC	4 cut(s)	82, 1442, 1699, 1825
PmaCI	CACGTG	1 cut(s)	1826
PmlI	CACGTG	1 cut(s)	1826
PpsI	GAGTC	4 cut(s)	82, 1442, 1699, 1825
Ppu21I	YACGTR	2 cut(s)	1826, 2204
PscI	ACATGT	1 cut(s)	2315
PshAI	GACNNNNGTC	1 cut(s)	1542
Psp6I	CCWGG	6 cut(s)	135, 346, 752, 935, 969, 1317
PspCI	CACGTG	1 cut(s)	1826
PspFI	CCCAGC	3 cut(s)	539, 2507, 2523
PspGI	CCWGG	6 cut(s)	135, 346, 752, 935, 969, 1317
PspLI	CGTACG	1 cut(s)	2204
PspPI	GGNCC	9 cut(s)	316, 626, 1126, 1271, 1478, 1537, 1656, 1938, 2241
PstI	CTGCAG	1 cut(s)	2421
PstNI	CAGNNNCTG	1 cut(s)	520
PsuI	RGATCY	3 cut(s)	771, 952, 2644
PsyI	GACNNNGTC	2 cut(s)	137, 1501
PvuII	CAGCTG	1 cut(s)	1052
RsaI	GTAC	9 cut(s)	255, 260, 746, 878, 1634, 1850, 2071, 2111, 2206
RsaNI	GTAC	9 cut(s)	254, 259, 745, 877, 1633, 1849, 2070, 2110, 2205
RseI	CAYNNNNRTG	6 cut(s)	677, 740, 987, 1664, 1873, 1946
SapI	GCTCTTC	2 cut(s)	392, 1964
SaqAI	TTAA	4 cut(s)	480, 495, 779, 1155
SatI	GCNGC	8 cut(s)	247, 612, 1519, 1575, 1908, 2310, 2375, 2420
Sau96I	GGNCC	9 cut(s)	316, 626, 1126, 1271, 1478, 1537, 1656, 1938, 2241
ScaI	AGTACT	1 cut(s)	2111
SchI	GAGTC	4 cut(s)	83, 1442, 1700, 1825
ScrFI	CCNGG	8 cut(s)	137, 315, 348, 754, 937, 971, 1319, 2105
SduI	GDGCHC	4 cut(s)	1230, 1708, 1976, 2042
SexAI	ACCWGGT	2 cut(s)	135, 969
SfaNI	GCATC	7 cut(s)	445, 449, 465, 534, 1201, 1722, 2494
SfcI	CTRYAG	5 cut(s)	189, 415, 948, 2113, 2417
SfuI	TTCGAA	1 cut(s)	2543
SgrAI	CRCCGGYG	1 cut(s)	1373
SinI	GGWCC	5 cut(s)	1126, 1271, 1537, 1656, 2241
SmiMI	CAYNNNNRTG	6 cut(s)	677, 740, 987, 1664, 1873, 1946
SmlI	CTYRAG	2 cut(s)	467, 2405
SmoI	CTYRAG	2 cut(s)	467, 2405
SnaBI	TACGTA	1 cut(s)	2204
SpeI	ACTAGT	2 cut(s)	2249, 2567
Sse9I	AATT	7 cut(s)	115, 287, 485, 576, 836, 959, 1893
SspI	AATATT	1 cut(s)	784
SspMI	CTAG	4 cut(s)	630, 1329, 2250, 2568
StyD4I	CCNGG	8 cut(s)	135, 313, 346, 752, 935, 969, 1317, 2103
StyI	CCWWGG	1 cut(s)	2007
TaaI	ACNGT	6 cut(s)	231, 258, 1533, 2074, 2127, 2653
TaiI	ACGT	9 cut(s)	705, 876, 1632, 1828, 1968, 2122, 2206, 2394, 2460
TaqII	GACCGA	3 cut(s)	113, 1375, 1736
TasI	AATT	7 cut(s)	115, 287, 485, 576, 836, 959, 1893
TatI	WGTACW	4 cut(s)	1632, 1848, 2069, 2109
TauI	GCSGC	5 cut(s)	614, 1521, 1577, 1910, 2377
Tru1I	TTAA	4 cut(s)	480, 495, 779, 1155
Tru9I	TTAA	4 cut(s)	480, 495, 779, 1155
TscAI	CASTG	9 cut(s)	454, 613, 666, 865, 1563, 1789, 2079, 2421, 2656
TseFI	GTSAC	1 cut(s)	1547
TseI	GCWGC	3 cut(s)	246, 2309, 2419
Tsp45I	GTSAC	1 cut(s)	1547
TspGWI	ACGGA	3 cut(s)	779, 1393, 1863
TspRI	CASTG	9 cut(s)	454, 613, 666, 865, 1563, 1789, 2079, 2421, 2656
Tth111I	GACNNNGTC	2 cut(s)	137, 1501
Van91I	CCANNNNNTGG	1 cut(s)	217
VpaK11BI	GGWCC	5 cut(s)	1126, 1271, 1537, 1656, 2241
XagI	CCTNNNNNAGG	1 cut(s)	2293
XapI	RAATTY	4 cut(s)	287, 485, 836, 1893
XceI	RCATGY	3 cut(s)	745, 2319, 2617
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1872, 1898
XmiI	GTMKAC	2 cut(s)	870, 2148
XmnI	GAANNNNTTC	1 cut(s)	2327
XspI	CTAG	4 cut(s)	630, 1329, 2250, 2568
ZraI	GACGTC	1 cut(s)	2120
ZrmI	AGTACT	1 cut(s)	2111

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.