Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0000121

Physical Location & Seq

Genomic Coordinates Forward (+)

4904100 .. 4906628

Gene Structure 2529 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1060300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2529 bp

ATGAACAACCCTATCAGCAAACACCCTAATAAAACTCTCACTCCTCTTCTCCTTCCCCTTTTTTTCCACATGATAACCATGTGCATGGCCGTGGACTCGCCGCCAATCTACCGTCCGTCTGAACAAATCACCCTTCAATGTGGCTCTTCCGGTACCAAATTCGGCCAAGACTCCCGATATTGGGATGGAGATATTGACACATCTTTTTTTCCATTCGGAGCAGCTGGTAACAAATCCATATTCAAAGAAGCACCTTCTCCCTCTTCTTCCGGCCAAGTGCCTTACAGCACAGCAAGGCTTTCCCGCTTTGAATTTACCTACACATTTCCACTCTCTTCCGGCCCAAAGTTCATCCGCTTGTATTTCTACCCAGCATCATACGCCCCCAACTTCAACCGATCCCAAGCCCTCTTCTCAGTCAAAGCCGGTGGCTTTACCCTTCTCCACGACTTCAACGCTTCAGCCACGGCTGATGCTTCTGCCTCGGATACTATATACAGAGAGTTCTGCCTCCACACCGACTCAGGACAGAATTTGAGTATCACGTTCACTCCAAGCAAAGCAAGCCCAGATGCCTACGCGTTTATCAATGGGATTGAAATTATCTCCATGCCCCCATATCTTTACTACACTTCACCCCAGAGCCCAGTTGGGGTTGCTTATGTAGGCAGCAAAAACACGATTCGCATCGAGAACAACACTGCTCTGGAGATGGTGTACCGAATCAATATCGGTGACAAAAGGCGGATCTATTCCGATCAGGACACTGGTATGTACCGCAATTGGGATAGTTTGGGAGACGAGTACAACTACTTAGACGATTTAAGTAGGAAGTTTAGTGTTTTACCACAGAACAGTAGCGTTCAGCTTGACTTTTCTGCAATACCAGAGTACTCTGCTCCGAAAGAAGTTTACCAAACCGGCCGCTCAATGGGCATGAACAAGACCATAAACAAGAGCTACAATCTCACCTGGGAATTCCCTGTGGATTCAATGTTTGCTTACTTGGTTAGGCTTCATTTTTGTGAGTTTGAAGGTGATGTTACGATGCCCCAAGACAGGCTATTTCATATCTACATCTCCAATCAATCCGTTGAGCAAATGGCAGATATAATCATGTGGAGCGGTGGAAATGGAAGACCAGTGTACAGAGACTACGTTGTGTTCATGTCGGGGAACCCTGCAATCCCAAAGAAAGTCAATCTCTTTCTTGCGCTGGAAGCAAGCAAGCAGGATTGGTTGACTAATTACAACGACGCAATCTTGAACGGACTCGAAATGTTCAAACTCAGTACAAATCAGAATCTCGCCAGACAAAGCCCCGACCCACCTCCTATAGCCCCAACAAGGGGGACATCAAACCCAAGCACCAGGTCAAGAACTCCTTTGCTTGCCACGGTCATTGGTGTAGCTTCCGGCATACTTGGACTCTCTGTTCTTGGGTTCTTGGTTTTTAGGCGGTGGCAGAAAGTCAAGGACATTGGCTCAGATGGGTCCTCATCTTTACCACCACATTTGTGTCGTTGCTTTTCACTGTCGGAGATCAAAGCCGCCACCCAAAACTTCAGCCAGGGTTTCATTGTTGGTGTTGGCGGCTTTGGTCACGTGTACAAAGGGCATATCGACGGCGGGGCCACTCCCGTTGCGATCAAACGGCTGAAACCCGAGTCAAAGCAGGGAGCCCGTGAGTTCAAGACGGAAATCGAACTGCTCTCACAACTCAGACACCGCCATTTGGTATCTCTCATTGGGTATTGTACTGATAAAAATGAGATGATTTTGGTGTACGATTACATGTCACATGGGACACTCGCTGACCATCTCTACCACAACGACAACCCACCTCTCTCCTGGGGACAACGGCTCCAAATTTGCATTGGGGCCGCGCGAGGGTTGCGCTACCTTCACAGCAATGCGCAGGGCACTATCATCCACCGTGACGTGAAGAGCACAAACATTTTATTGGATGAGAAATGGGTGGCCAAGGTTTCGGATTTTGGATTGTCGAAAATGGGCACGACCACCATGTCCAAGACCCACATTAGCACGGCCGTGAAAGGCAGTTTCGGTTATCTAGACCCGGAATACTACCGACGTCAAAAACTAACGGTGAAGTCCGATGTGTACTCATTCGGTGTAGTGTTGTGTGAAGTACTGTGCTCAAGACCAGCTGTGGTGCATGCAGTGGAGACAAGGCAAATGAACTTGGCTGAATGGGCCAAGAGCTGCCATCGCGACAGGGAACTTGATCAAATTATTGATCCGAACATGAAGGGTAAGATTGAAACCGAGTGTTTGAACAAGTTTGTGGAAATTGCTATGAGTTGCATCAATGATAATGGGATAGAGCGGCCGACAATGAACGATGTTGTGAGGGGGCTTGAGTTTGCATTGCAACTACATCAGAAGTGCGGTGAAAGGAACGACGAGGATGACTTGGCTAGTGTGCCAGGTTGCGCTACGAATGAATCCGTTCAGTGCATATCCGGGACGATCTTCTCAGAAATCAACAATTCGAATGGGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009723 GO:0009725 GO:0009741 GO:0009787 GO:0009788 GO:0009791 GO:0009856 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010118 GO:0010483 GO:0010646 GO:0010648 GO:0014070 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022622 GO:0023051 GO:0023057 GO:0030054 GO:0030308 GO:0030312 GO:0032501 GO:0032502 GO:0033993 GO:0036211 GO:0040008 GO:0042221 GO:0043170 GO:0043207 GO:0043412 GO:0043680 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044426 GO:0044462 GO:0044464 GO:0044703 GO:0044706 GO:0045926 GO:0046777 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051128 GO:0051703 GO:0051704 GO:0051707 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:0098542 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1905957 GO:1905958

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Malectin_like Pkinase_Tyr

Protein Analysis

843

Amino Acids

94.02

Weight (kDa)

7.26

Isoelectric Point (pI)

46.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Malectin_like	PF12819	66 - 429	5.1e-28	Malectin-like domain
Malectin	PF11721	239 - 389	1.1e-08	Malectin domain
Pkinase	PF00069	527 - 791	3.6e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	528 - 793	1.3e-49	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000121)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g46570 FvH4_6g46580 FvH4_6g46610 FvH4_6g46620 FvH4_6g46640 FvH4_6g46680
malus_domestica	MD03G1063600.v1.1 MD03G1063700.v1.1 MD09G1069300.v1.1 MD09G1069400.v1.1 MD11G1067200.v1.1 MD11G1070300.v1.1 MD11G1153300.v1.1 MD17G1060300.v1.1 MD17G1061900.v1.1 MD17G1062100.v1.1 MD17G1062300.v1.1 MD17G1062400.v1.1 MD17G1063000.v1.1 MD17G1063200.v1.1 MD17G1063600.v1.1
prunus_persica	Prupe.3G246200_v2.0.a1 Prupe.3G251000_v2.0.a1 Prupe.3G251100_v2.0.a1 Prupe.3G251200_v2.0.a1 Prupe.3G251600_v2.0.a1 Prupe.3G251700_v2.0.a1 Prupe.3G251800_v2.0.a1 Prupe.3G251900_v2.0.a1
pyrus_communis	pycom03g05070 pycom03g05100 pycom12433g00070 pycom17g06000 pycom17g06170 pycom17g06180 pycom17g06200 pycom17g06210 pycom17g06280 pycom17g06300 pycom17g06310 pycom17g06320 pycom17g06330 pycom17g06350 pycom17g06370 pycom17g06380 pycom17g06400
rosa_chinensis	RchiOBHm_Chr1g0321851 RchiOBHm_Chr1g0321911 RchiOBHm_Chr1g0323951 RchiOBHm_Chr1g0361941 RchiOBHm_Chr2g0164711 RchiOBHm_Chr2g0165301 RchiOBHm_Chr2g0165311 RchiOBHm_Chr2g0165321 RchiOBHm_Chr2g0165351 RchiOBHm_Chr2g0165431 RchiOBHm_Chr2g0165461 RchiOBHm_Chr2g0165491 RchiOBHm_Chr2g0165511 RchiOBHm_Chr2g0165521 RchiOBHm_Chr2g0165531 RchiOBHm_Chr3g0481631 RchiOBHm_Chr3g0481801 RchiOBHm_Chr3g0481811 RchiOBHm_Chr3g0481821 RchiOBHm_Chr3g0481831 RchiOBHm_Chr4g0400291 RchiOBHm_Chr4g0400371 RchiOBHm_Chr4g0400391 RchiOBHm_Chr6g0274711 RchiOBHm_Chr6g0274721 RchiOBHm_Chr7g0211011
rosa_laevigata	RLG00000002260 RLG00000002262 RLG00000002263 RLG00000003027 RLG00000003028 RLG00000009196 RLG00000009197 RLG00000012573 RLG00000020400 RLG00000021523 RLG00000021524 RLG00000021525 RLG00000021527 RLG00000021528 RLG00000021529 RLG00000021530 RLG00000021532 RLG00000021533 RLG00000021534 RLG00000021535 RLG00000021537 RLG00000023384 RLG00000027681 RLG00000027682 RLG00000027685 RLG00000030240 RLG00000030401
rosa_multiflora	Rmu_co7998078.1_g000001 Rmu_co8009032.1_g000001 Rmu_co8326071.1_g000001 Rmu_co8333709.1_g000001 Rmu_co8362007.1_g000001 Rmu_co8369261.1_g000001 Rmu_co8391219.1_g000001 Rmu_co8456951.1_g000001 Rmu_co8461523.1_g000001 Rmu_sc0000427.1_g000009 Rmu_sc0000427.1_g000032 Rmu_sc0000427.1_g000033 Rmu_sc0000521.1_g000005 Rmu_sc0000521.1_g000008 Rmu_sc0000521.1_g000009 Rmu_sc0000655.1_g000016 Rmu_sc0000655.1_g000017 Rmu_sc0000655.1_g000018 Rmu_sc0000675.1_g000003 Rmu_sc0000795.1_g000018 Rmu_sc0000795.1_g000083 Rmu_sc0001306.1_g000042 Rmu_sc0001306.1_g000043 Rmu_sc0001873.1_g000001 Rmu_sc0001873.1_g000010 Rmu_sc0002529.1_g000002 Rmu_sc0002529.1_g000004 Rmu_sc0003410.1_g000004 Rmu_sc0003909.1_g000010 Rmu_sc0004097.1_g000001 Rmu_sc0005425.1_g000003 Rmu_sc0005425.1_g000004 Rmu_sc0005425.1_g000010 Rmu_sc0005425.1_g000012 Rmu_sc0005425.1_g000017 Rmu_sc0006223.1_g000014 Rmu_sc0006399.1_g000007 Rmu_sc0010324.1_g000001 Rmu_sc0010409.1_g000005 Rmu_sc0013532.1_g000005 Rmu_sc0016601.1_g000002 Rmu_sc0016601.1_g000003 Rmu_sc0016601.1_g000006 Rmu_sc0017773.1_g000001 Rmu_sc0017773.1_g000002 Rmu_sc0019514.1_g000002 Rmu_sc0021575.1_g000001 Rmu_sc0023720.1_g000002 Rmu_sc0030898.1_g000001 Rmu_sc0033982.1_g000001 Rmu_sc0034651.1_g000001 Rmu_sc0038482.1_g000001 Rmu_sc0038644.1_g000001 Rmu_sc0038644.1_g000003 Rmu_sc0039243.1_g000001 Rmu_sc0042715.1_g000001 Rmu_ssc0000124.1_g000003
rosa_roxburghii	Rroxscaffold_2G00085740 Rroxscaffold_2G00085760 Rroxscaffold_2G00085780 Rroxscaffold_2G00085800 Rroxscaffold_2G00085830 Rroxscaffold_2G00085850 Rroxscaffold_2G00085860 Rroxscaffold_4G00294610 Rroxscaffold_4G00326070 Rroxscaffold_4G00326080 Rroxscaffold_4G00327420 Rroxscaffold_4G00327430 Rroxscaffold_4G00327460 Rroxscaffold_4G00327470 Rroxscaffold_5G00345350 Rroxscaffold_6G00399890 Rroxscaffold_6G00400250 Rroxscaffold_7G00194000 Rroxscaffold_7G00194020 Rroxscaffold_7G00194050
rosa_rugosa	Rorug01G0034300 Rorug01G0034500 Rorug01G0040700 Rorug01G0064300 Rorug02G0513700 Rorug02G0513800 Rorug02G0513900 Rorug02G0514000 Rorug02G0514000 Rorug02G0514100 Rorug02G0514300 Rorug02G0514400 Rorug02G0514700 Rorug02G0515000 Rorug02G0515100 Rorug02G0515300 Rorug02G0515500 Rorug02G0515600 Rorug02G0515800 Rorug02G0515900 Rorug02G0516000 Rorug03G0193200 Rorug04G0024600 Rorug06G0083100 Rorug06G0085200 Rorug07G0188600
rosa_samantha	Rh1CG234000 Rh2DG603700 Rh2DG603800 Rh2DG603900 Rh6BG203000
rosa_wichuraiana	Rw0G010290 Rw1G004240 Rw1G005000 Rw1G026960 Rw2G048310 Rw2G048320 Rw2G048330 Rw2G048350 Rw2G048360 Rw2G048380 Rw2G048430 Rw2G048460 Rw2G048500 Rw2G048510 Rw2G048520 Rw2G048530 Rw3G021810 Rw4G008270 Rw6G017320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2026
AatII	GACGTC	1 cut(s)	2098
Acc16I	TGCGCA	1 cut(s)	1917
Acc65I	GGTACC	1 cut(s)	152
AccB1I	GGYRCC	1 cut(s)	152
AccBSI	CCGCTC	3 cut(s)	927, 1125, 2350
AccII	CGCG	4 cut(s)	581, 1886, 1888, 2235
AclWI	GGATC	3 cut(s)	393, 755, 2255
AcoI	YGGCCR	7 cut(s)	87, 163, 271, 922, 1980, 2049, 2351
AcsI	RAATTY	5 cut(s)	158, 311, 532, 977, 1869
AcuI	CTGAAG	2 cut(s)	444, 1549
AcvI	CACGTG	1 cut(s)	1606
AcyI	GRCGYC	1 cut(s)	2095
AflIII	ACRYGT	3 cut(s)	579, 1605, 1794
AjiI	CACGTC	1 cut(s)	1942
AjnI	CCWGG	5 cut(s)	971, 1370, 1569, 1850, 2449
AleI	CACNNNNGTG	2 cut(s)	1516, 2051
AloI	GAACNNNNNNTCC	2 cut(s)	1419, 1451
AluBI	AGCT	6 cut(s)	224, 868, 960, 1412, 2171, 2226
AluI	AGCT	6 cut(s)	224, 868, 960, 1412, 2171, 2226
Alw21I	GWGCWC	2 cut(s)	1952, 2162
Alw26I	GTCTC	3 cut(s)	792, 1146, 2183
AlwI	GGATC	3 cut(s)	393, 755, 2255
Ama87I	CYCGRG	1 cut(s)	1664
ApeKI	GCWGC	3 cut(s)	221, 669, 2226
ApoI	RAATTY	5 cut(s)	158, 311, 532, 977, 1869
Asp700I	GAANNNNTTC	1 cut(s)	2472
Asp718I	GGTACC	1 cut(s)	152
AspLEI	GCGC	5 cut(s)	1216, 1888, 1899, 1918, 2459
AspS9I	GGNCC	5 cut(s)	341, 1494, 1632, 1881, 2217
AsuC2I	CCSGG	2 cut(s)	2081, 2488
AsuHPI	GGTGA	7 cut(s)	121, 627, 746, 961, 1049, 2122, 2426
AsuII	TTCGAA	1 cut(s)	2516
AvaI	CYCGRG	1 cut(s)	1664
AvaII	GGWCC	1 cut(s)	1494
BaeGI	GKGCMC	2 cut(s)	1925, 2018
BalI	TGGCCA	1 cut(s)	1982
BanI	GGYRCC	1 cut(s)	152
BanII	GRGCYC	2 cut(s)	647, 1684
BbrPI	CACGTG	1 cut(s)	1606
BbsI	GAAGAC	1 cut(s)	1144
Bbv12I	GWGCWC	2 cut(s)	1952, 2162
BbvI	GCAGC	3 cut(s)	233, 681, 2213
BccI	CCATC	5 cut(s)	179, 706, 1484, 1827, 2238
BceAI	ACGGC	7 cut(s)	74, 483, 1642, 1670, 1877, 2036, 2064
BciT130I	CCWGG	5 cut(s)	973, 1372, 1571, 1852, 2451
BciVI	GTATCC	1 cut(s)	481
BclI	TGATCA	1 cut(s)	2248
BcnI	CCSGG	2 cut(s)	2081, 2488
BcoDI	GTCTC	3 cut(s)	792, 1146, 2183
BfaI	CTAG	2 cut(s)	2075, 2442
BfmI	CTRYAG	1 cut(s)	1335
BfuI	GTATCC	1 cut(s)	481
BisI	GCNGC	9 cut(s)	101, 222, 670, 925, 1551, 1594, 1884, 2227, 2351
BlsI	GCNGC	9 cut(s)	102, 223, 671, 926, 1552, 1595, 1885, 2228, 2352
BmcAI	AGTACT	2 cut(s)	893, 2154
Bme1390I	CCNGG	7 cut(s)	973, 1372, 1571, 1852, 2081, 2451, 2488
Bme18I	GGWCC	1 cut(s)	1494
BmeT110I	CYCGRG	1 cut(s)	1664
BmgBI	CACGTC	1 cut(s)	1942
BmgT120I	GGNCC	5 cut(s)	341, 1494, 1632, 1881, 2217
BmiI	GGNNCC	7 cut(s)	154, 1178, 1495, 1633, 1681, 1865, 1882
BmrFI	CCNGG	7 cut(s)	973, 1372, 1571, 1852, 2081, 2451, 2488
BmrI	ACTGGG	1 cut(s)	641
BmsI	GCATC	6 cut(s)	383, 463, 562, 696, 1038, 2337
BmuI	ACTGGG	1 cut(s)	641
BpiI	GAAGAC	1 cut(s)	1144
BpmI	CTGGAG	1 cut(s)	728
Bpu14I	TTCGAA	1 cut(s)	2516
BpuEI	CTTGAG	2 cut(s)	2146, 2402
BpuMI	CCSGG	2 cut(s)	2081, 2488
BsaAI	YACGTR	1 cut(s)	1606
BsaBI	GATNNNNATC	1 cut(s)	686
BsaHI	GRCGYC	1 cut(s)	2095
BsaJI	CCNNGG	8 cut(s)	90, 465, 483, 972, 1395, 1570, 1851, 1983
BsaWI	WCCGGW	1 cut(s)	149
BsaXI	ACNNNNNCTCC	8 cut(s)	25, 55, 535, 565, 701, 731, 1848, 1878
Bse118I	RCCGGY	2 cut(s)	425, 920
Bse1I	ACTGG	3 cut(s)	647, 772, 1142
Bse3DI	GCAATG	2 cut(s)	1918, 2390
Bse8I	GATNNNNATC	1 cut(s)	686
BseBI	CCWGG	5 cut(s)	973, 1372, 1571, 1852, 2451
BseDI	CCNNGG	8 cut(s)	90, 465, 483, 972, 1395, 1570, 1851, 1983
BseGI	GGATG	5 cut(s)	190, 351, 1929, 1972, 2437
BseJI	GATNNNNATC	1 cut(s)	686
BseMI	GCAATG	2 cut(s)	1918, 2390
BseMII	CTCAG	6 cut(s)	429, 537, 1303, 1500, 1735, 2514
BseNI	ACTGG	3 cut(s)	647, 772, 1142
BseRI	GAGGAG	1 cut(s)	33
BseSI	GKGCMC	2 cut(s)	1925, 2018
BseX3I	CGGCCG	3 cut(s)	922, 2049, 2351
BseXI	GCAGC	3 cut(s)	233, 681, 2213
BseYI	CCCAGC	1 cut(s)	370
Bsh1236I	CGCG	4 cut(s)	581, 1886, 1888, 2235
Bsh1285I	CGRYCG	3 cut(s)	925, 2052, 2354
BshNI	GGYRCC	1 cut(s)	152
BsiEI	CGRYCG	3 cut(s)	925, 2052, 2354
BsiHKAI	GWGCWC	2 cut(s)	1952, 2162
BsiHKCI	CYCGRG	1 cut(s)	1664
BsiSI	CCGG	8 cut(s)	150, 270, 339, 426, 921, 1416, 2081, 2487
BslFI	GGGAC	4 cut(s)	1366, 1819, 1869, 2503
BsmAI	GTCTC	3 cut(s)	792, 1146, 2183
BsmBI	CGTCTC	1 cut(s)	792
BsmFI	GGGAC	4 cut(s)	1366, 1819, 1869, 2503
BsoBI	CYCGRG	1 cut(s)	1664
Bsp119I	TTCGAA	1 cut(s)	2516
Bsp1286I	GDGCHC	6 cut(s)	647, 1684, 1925, 1952, 2018, 2162
Bsp1407I	TGTACA	2 cut(s)	1146, 1608
Bsp143I	GATC	8 cut(s)	398, 747, 757, 1542, 1647, 2248, 2260, 2493
Bsp68I	TCGCGA	1 cut(s)	2235
BspCNI	CTCAG	6 cut(s)	428, 536, 1302, 1499, 1734, 2513
BspFNI	CGCG	4 cut(s)	581, 1886, 1888, 2235
BspLI	GGNNCC	7 cut(s)	154, 1178, 1495, 1633, 1681, 1865, 1882
BspPI	GGATC	3 cut(s)	393, 755, 2255
BspQI	GCTCTTC	2 cut(s)	151, 1940
BspT104I	TTCGAA	1 cut(s)	2516
BspT107I	GGYRCC	1 cut(s)	152
BsrBI	CCGCTC	3 cut(s)	927, 1125, 2350
BsrDI	GCAATG	2 cut(s)	1918, 2390
BsrFI	RCCGGY	2 cut(s)	425, 920
BsrGI	TGTACA	2 cut(s)	1146, 1608
BsrI	ACTGG	3 cut(s)	647, 772, 1142
BssAI	RCCGGY	2 cut(s)	425, 920
BssECI	CCNNGG	8 cut(s)	90, 465, 483, 972, 1395, 1570, 1851, 1983
BssMI	GATC	8 cut(s)	398, 747, 757, 1542, 1647, 2248, 2260, 2493
BssNI	GRCGYC	1 cut(s)	2095
BssT1I	CCWWGG	1 cut(s)	1983
Bst2UI	CCWGG	5 cut(s)	973, 1372, 1571, 1852, 2451
Bst4CI	ACNGT	7 cut(s)	113, 857, 1399, 1536, 1937, 2110, 2157
Bst6I	CTCTTC	6 cut(s)	51, 151, 268, 340, 416, 1940
BstACI	GRCGYC	1 cut(s)	2095
BstAUI	TGTACA	2 cut(s)	1146, 1608
BstBAI	YACGTR	1 cut(s)	1606
BstBI	TTCGAA	1 cut(s)	2516
BstC8I	GCNNGC	5 cut(s)	565, 1225, 1229, 1392, 2181
BstDEI	CTNAG	7 cut(s)	415, 523, 814, 1289, 1486, 1721, 2500
BstDSI	CCRYGG	3 cut(s)	90, 465, 1395
BstF5I	GGATG	5 cut(s)	190, 351, 1929, 1972, 2437
BstFNI	CGCG	4 cut(s)	581, 1886, 1888, 2235
BstHHI	GCGC	5 cut(s)	1216, 1888, 1899, 1918, 2459
BstKTI	GATC	8 cut(s)	401, 750, 760, 1545, 1650, 2251, 2263, 2496
BstMAI	GTCTC	3 cut(s)	792, 1146, 2183
BstMBI	GATC	8 cut(s)	398, 747, 757, 1542, 1647, 2248, 2260, 2493
BstMCI	CGRYCG	3 cut(s)	925, 2052, 2354
BstMWI	GCNNNNNNNGC	7 cut(s)	380, 564, 933, 1220, 1894, 2216, 2232
BstNI	CCWGG	5 cut(s)	973, 1372, 1571, 1852, 2451
BstNSI	RCATGY	2 cut(s)	1798, 2183
BstSCI	CCNGG	7 cut(s)	971, 1370, 1569, 1850, 2079, 2449, 2486
BstSFI	CTRYAG	1 cut(s)	1335
BstSLI	GKGCMC	2 cut(s)	1925, 2018
BstUI	CGCG	4 cut(s)	581, 1886, 1888, 2235
BstV1I	GCAGC	3 cut(s)	233, 681, 2213
BstV2I	GAAGAC	1 cut(s)	1144
BstX2I	RGATCY	1 cut(s)	747
BstXI	CCANNNNNNTGG	1 cut(s)	85
BstYI	RGATCY	1 cut(s)	747
BstZI	CGGCCG	3 cut(s)	922, 2049, 2351
BsuI	GTATCC	1 cut(s)	481
BtgI	CCRYGG	3 cut(s)	90, 465, 1395
BtgZI	GCGATG	1 cut(s)	2216
BtrI	CACGTC	1 cut(s)	1942
BtsCI	GGATG	5 cut(s)	190, 351, 1929, 1972, 2437
BtsI	GCAGTG	2 cut(s)	699, 2190
BtsIMutI	CAGTG	6 cut(s)	699, 765, 1149, 1532, 2190, 2483
BtuMI	TCGCGA	1 cut(s)	2235
Cac8I	GCNNGC	5 cut(s)	565, 1225, 1229, 1392, 2181
CfoI	GCGC	5 cut(s)	1216, 1888, 1899, 1918, 2459
Cfr10I	RCCGGY	2 cut(s)	425, 920
Cfr13I	GGNCC	5 cut(s)	341, 1494, 1632, 1881, 2217
CseI	GACGC	1 cut(s)	1265
CsiI	ACCWGGT	1 cut(s)	1370
CspCI	CAANNNNNGTGG	8 cut(s)	409, 444, 1384, 1419, 1497, 1532, 1624, 1659
DdeI	CTNAG	7 cut(s)	415, 523, 814, 1289, 1486, 1721, 2500
DpnI	GATC	8 cut(s)	400, 749, 759, 1544, 1649, 2250, 2262, 2495
DpnII	GATC	8 cut(s)	398, 747, 757, 1542, 1647, 2248, 2260, 2493
DrdI	GACNNNNNNGTC	1 cut(s)	2026
DseDI	GACNNNNNNGTC	1 cut(s)	2026
EaeI	YGGCCR	7 cut(s)	87, 163, 271, 922, 1980, 2049, 2351
EagI	CGGCCG	3 cut(s)	922, 2049, 2351
Eam1104I	CTCTTC	6 cut(s)	51, 151, 268, 340, 416, 1940
EarI	CTCTTC	6 cut(s)	51, 151, 268, 340, 416, 1940
EciI	GGCGGA	1 cut(s)	760
EclXI	CGGCCG	3 cut(s)	922, 2049, 2351
Eco130I	CCWWGG	1 cut(s)	1983
Eco24I	GRGCYC	2 cut(s)	647, 1684
Eco47I	GGWCC	1 cut(s)	1494
Eco52I	CGGCCG	3 cut(s)	922, 2049, 2351
Eco57I	CTGAAG	2 cut(s)	444, 1549
Eco72I	CACGTG	1 cut(s)	1606
Eco88I	CYCGRG	1 cut(s)	1664
EcoO109I	RGGNCCY	1 cut(s)	1494
EcoRI	GAATTC	1 cut(s)	977
EcoRII	CCWGG	5 cut(s)	971, 1370, 1569, 1850, 2449
EcoT14I	CCWWGG	1 cut(s)	1983
EcoT38I	GRGCYC	2 cut(s)	647, 1684
ErhI	CCWWGG	1 cut(s)	1983
Esp3I	CGTCTC	1 cut(s)	792
FalI	AAGNNNNNCTT	2 cut(s)	1369, 1401
FaqI	GGGAC	4 cut(s)	1366, 1819, 1869, 2503
FauI	CCCGC	2 cut(s)	311, 1622
FbaI	TGATCA	1 cut(s)	2248
Fnu4HI	GCNGC	9 cut(s)	101, 222, 670, 925, 1551, 1594, 1884, 2227, 2351
FokI	GGATG	5 cut(s)	197, 338, 1916, 1979, 2444
FriOI	GRGCYC	2 cut(s)	647, 1684
Fsp4HI	GCNGC	9 cut(s)	101, 222, 670, 925, 1551, 1594, 1884, 2227, 2351
FspBI	CTAG	2 cut(s)	2075, 2442
FspI	TGCGCA	1 cut(s)	1917
GlaI	GCGC	5 cut(s)	1215, 1887, 1898, 1917, 2458
GluI	GCNGC	9 cut(s)	101, 222, 670, 925, 1551, 1594, 1884, 2227, 2351
GsaI	CCCAGC	1 cut(s)	374
GsuI	CTGGAG	1 cut(s)	728
HapII	CCGG	8 cut(s)	150, 270, 339, 426, 921, 1416, 2081, 2487
HgaI	GACGC	1 cut(s)	1265
HhaI	GCGC	5 cut(s)	1216, 1888, 1899, 1918, 2459
Hin1I	GRCGYC	1 cut(s)	2095
Hin6I	GCGC	5 cut(s)	1214, 1886, 1897, 1916, 2457
HinP1I	GCGC	5 cut(s)	1214, 1886, 1897, 1916, 2457
HincII	GTYRAC	1 cut(s)	1242
HindII	GTYRAC	1 cut(s)	1242
HpaII	CCGG	8 cut(s)	150, 270, 339, 426, 921, 1416, 2081, 2487
HphI	GGTGA	7 cut(s)	121, 627, 746, 961, 1049, 2122, 2426
Hpy166II	GTNNAC	9 cut(s)	94, 549, 718, 913, 1147, 1242, 1609, 1786, 2125
Hpy8I	GTNNAC	9 cut(s)	94, 549, 718, 913, 1147, 1242, 1609, 1786, 2125
Hpy99I	CGWCG	4 cut(s)	1259, 1628, 2097, 2429
HpyAV	CCTTC	7 cut(s)	62, 143, 264, 449, 1028, 1913, 2266
HpyCH4III	ACNGT	7 cut(s)	113, 857, 1399, 1536, 1937, 2110, 2157
HpyCH4IV	ACGT	5 cut(s)	545, 1158, 1605, 1941, 2095
HpyF10VI	GCNNNNNNNGC	7 cut(s)	380, 564, 933, 1220, 1894, 2216, 2232
HpyF3I	CTNAG	7 cut(s)	415, 523, 814, 1289, 1486, 1721, 2500
HpySE526I	ACGT	5 cut(s)	545, 1158, 1605, 1941, 2095
Hsp92I	GRCGYC	1 cut(s)	2095
HspAI	GCGC	5 cut(s)	1214, 1886, 1897, 1916, 2457
KpnI	GGTACC	1 cut(s)	156
Ksp22I	TGATCA	1 cut(s)	2248
Kzo9I	GATC	8 cut(s)	398, 747, 757, 1542, 1647, 2248, 2260, 2493
LguI	GCTCTTC	2 cut(s)	151, 1940
LmnI	GCTCC	5 cut(s)	218, 904, 1122, 1679, 1869
Lsp1109I	GCAGC	3 cut(s)	233, 681, 2213
LweI	GCATC	6 cut(s)	383, 463, 562, 696, 1038, 2337
MabI	ACCWGGT	1 cut(s)	1370
MaeI	CTAG	2 cut(s)	2075, 2442
MaeII	ACGT	5 cut(s)	545, 1158, 1605, 1941, 2095
MaeIII	GTNAC	6 cut(s)	227, 734, 1042, 1601, 1797, 1937
MalI	GATC	8 cut(s)	400, 749, 759, 1544, 1649, 2250, 2262, 2495
MbiI	CCGCTC	3 cut(s)	927, 1125, 2350
MboI	GATC	8 cut(s)	398, 747, 757, 1542, 1647, 2248, 2260, 2493
MboII	GAAGA	9 cut(s)	38, 138, 255, 258, 327, 403, 1149, 1957, 2488
MfeI	CAATTG	1 cut(s)	781
MflI	RGATCY	1 cut(s)	747
MhlI	GDGCHC	6 cut(s)	647, 1684, 1925, 1952, 2018, 2162
MlsI	TGGCCA	1 cut(s)	1982
MluI	ACGCGT	1 cut(s)	579
MluNI	TGGCCA	1 cut(s)	1982
MlyI	GAGTC	6 cut(s)	89, 164, 515, 1266, 1422, 1676
MmeI	TCCRAC	1 cut(s)	1518
Mox20I	TGGCCA	1 cut(s)	1982
MroXI	GAANNNNTTC	1 cut(s)	2472
MscI	TGGCCA	1 cut(s)	1982
MseI	TTAA	1 cut(s)	824
MslI	CAYNNNNRTG	7 cut(s)	83, 89, 770, 1023, 1516, 1911, 2051
Msp20I	TGGCCA	1 cut(s)	1982
MspA1I	CMGCKG	2 cut(s)	224, 2171
MspI	CCGG	8 cut(s)	150, 270, 339, 426, 921, 1416, 2081, 2487
MspR9I	CCNGG	7 cut(s)	973, 1372, 1571, 1852, 2081, 2451, 2488
MunI	CAATTG	1 cut(s)	781
MvaI	CCWGG	5 cut(s)	973, 1372, 1571, 1852, 2451
MvnI	CGCG	4 cut(s)	581, 1886, 1888, 2235
MwoI	GCNNNNNNNGC	7 cut(s)	380, 564, 933, 1220, 1894, 2216, 2232
NciI	CCSGG	2 cut(s)	2081, 2488
NdeII	GATC	8 cut(s)	398, 747, 757, 1542, 1647, 2248, 2260, 2493
NlaIV	GGNNCC	7 cut(s)	154, 1178, 1495, 1633, 1681, 1865, 1882
NmuCI	GTSAC	4 cut(s)	734, 1601, 1797, 1937
NruI	TCGCGA	1 cut(s)	2235
NsbI	TGCGCA	1 cut(s)	1917
NspI	RCATGY	2 cut(s)	1798, 2183
NspV	TTCGAA	1 cut(s)	2516
OliI	CACNNNNGTG	2 cut(s)	1516, 2051
PaeI	GCATGC	1 cut(s)	2183
PciI	ACATGT	1 cut(s)	1794
PciSI	GCTCTTC	2 cut(s)	151, 1940
PdmI	GAANNNNTTC	1 cut(s)	2472
PfeI	GAWTC	5 cut(s)	682, 723, 989, 1303, 2468
PfoI	TCCNGGA	1 cut(s)	2486
PkrI	GCNGC	9 cut(s)	102, 223, 671, 926, 1552, 1595, 1885, 2228, 2352
PleI	GAGTC	6 cut(s)	89, 164, 515, 1266, 1422, 1675
PmaCI	CACGTG	1 cut(s)	1606
PmlI	CACGTG	1 cut(s)	1606
PpsI	GAGTC	6 cut(s)	89, 164, 515, 1266, 1422, 1675
Ppu21I	YACGTR	1 cut(s)	1606
PpuMI	RGGWCCY	1 cut(s)	1494
PscI	ACATGT	1 cut(s)	1794
Psp5II	RGGWCCY	1 cut(s)	1494
Psp6I	CCWGG	5 cut(s)	971, 1370, 1569, 1850, 2449
PspCI	CACGTG	1 cut(s)	1606
PspFI	CCCAGC	1 cut(s)	370
PspGI	CCWGG	5 cut(s)	971, 1370, 1569, 1850, 2449
PspN4I	GGNNCC	7 cut(s)	154, 1178, 1495, 1633, 1681, 1865, 1882
PspPI	GGNCC	5 cut(s)	341, 1494, 1632, 1881, 2217
PspPPI	RGGWCCY	1 cut(s)	1494
PsuI	RGATCY	1 cut(s)	747
PvuII	CAGCTG	2 cut(s)	224, 2171
RruI	TCGCGA	1 cut(s)	2235
RseI	CAYNNNNRTG	7 cut(s)	83, 89, 770, 1023, 1516, 1911, 2051
SapI	GCTCTTC	2 cut(s)	151, 1940
SaqAI	TTAA	1 cut(s)	824
SatI	GCNGC	9 cut(s)	101, 222, 670, 925, 1551, 1594, 1884, 2227, 2351
Sau3AI	GATC	8 cut(s)	398, 747, 757, 1542, 1647, 2248, 2260, 2493
Sau96I	GGNCC	5 cut(s)	341, 1494, 1632, 1881, 2217
ScaI	AGTACT	2 cut(s)	893, 2154
SchI	GAGTC	6 cut(s)	89, 164, 515, 1266, 1422, 1676
ScrFI	CCNGG	7 cut(s)	973, 1372, 1571, 1852, 2081, 2451, 2488
SduI	GDGCHC	6 cut(s)	647, 1684, 1925, 1952, 2018, 2162
SexAI	ACCWGGT	1 cut(s)	1370
SfaNI	GCATC	6 cut(s)	383, 463, 562, 696, 1038, 2337
SfcI	CTRYAG	1 cut(s)	1335
SfuI	TTCGAA	1 cut(s)	2516
SinI	GGWCC	1 cut(s)	1494
SmiMI	CAYNNNNRTG	7 cut(s)	83, 89, 770, 1023, 1516, 1911, 2051
SmlI	CTYRAG	2 cut(s)	2161, 2381
SmoI	CTYRAG	2 cut(s)	2161, 2381
SphI	GCATGC	1 cut(s)	2183
SspMI	CTAG	2 cut(s)	2075, 2442
StyD4I	CCNGG	7 cut(s)	971, 1370, 1569, 1850, 2079, 2449, 2486
StyI	CCWWGG	1 cut(s)	1983
TaaI	ACNGT	7 cut(s)	113, 857, 1399, 1536, 1937, 2110, 2157
TaiI	ACGT	5 cut(s)	548, 1161, 1608, 1944, 2098
TaqI	TCGA	6 cut(s)	690, 1275, 1623, 1704, 2006, 2516
TatI	WGTACW	8 cut(s)	804, 891, 1146, 1292, 1608, 1757, 2124, 2152
TauI	GCSGC	6 cut(s)	103, 927, 1553, 1596, 1886, 2353
TfiI	GAWTC	5 cut(s)	682, 723, 989, 1303, 2468
Tru1I	TTAA	1 cut(s)	824
Tru9I	TTAA	1 cut(s)	824
TscAI	CASTG	6 cut(s)	706, 772, 1149, 1539, 2190, 2483
TseFI	GTSAC	4 cut(s)	734, 1601, 1797, 1937
TseI	GCWGC	3 cut(s)	221, 669, 2226
Tsp45I	GTSAC	4 cut(s)	734, 1601, 1797, 1937
TspGWI	ACGGA	5 cut(s)	105, 1081, 1284, 1712, 2461
TspRI	CASTG	6 cut(s)	706, 772, 1149, 1539, 2190, 2483
VpaK11BI	GGWCC	1 cut(s)	1494
XapI	RAATTY	5 cut(s)	158, 311, 532, 977, 1869
XbaI	TCTAGA	1 cut(s)	2074
XceI	RCATGY	2 cut(s)	1798, 2183
XcmI	CCANNNNNNNNNTGG	3 cut(s)	647, 1848, 1874
XmnI	GAANNNNTTC	1 cut(s)	2472
XspI	CTAG	2 cut(s)	2075, 2442
ZraI	GACGTC	1 cut(s)	2096
ZrmI	AGTACT	2 cut(s)	893, 2154

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.