Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0000121

Physical Location & Seq

Genomic Coordinates Forward (+)

76094058 .. 76096764

Gene Structure 2707 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G048520.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2598 bp

ATGCCAAACATATCCAGAGTACCTTTCCTCCGAAATCCTAACCCTCTTTTTTTCTTGCTATTTACGATGAAACCAATTCTCACTCTGCTTTACCTTTTCCTGTTTGTGTATGTTGTCACCCTGTTGGTGGCCGGAGATTTGGCATCTACGTACACTCCGGTGGAAAATATCACCGTTGCTTGTGGCGCTTATGGGACTGTCACTCAGCATTATACAAGTTATGAGAATCTCACTGGCGCTTATGGCGGCACTTATAATTTAAGACATTGGAGTGGAGATATCAATTCGAAATTCTCGCCCATTGGCCACAGCACCACCACCCAAGTCAACACTTCGATTGAAGTACCATACGACACAGCGCGGCTTTCTCACTCGGAATTTACTTACAGATTCAATCTCACCGCCGGCCAAAAGTTCATACGGTTGTACTTCTACCCAGATTCCTACACCAACTTCAACCCCTCCAAAGCTCGCTTCTCCATCAAAGCCGGTGACTTTACCCTTCTTGACACTGCGGATGCTTCTGGGGAGGAGAAGTTTTACAGAGAATTCTGTTTGAACGTTGACGAAGAACAGAGTCTAAAGACTTTCAACATCACATTCAGTCCAAGCAAAGATGCCTACGCATTTATCAACGGTATTGAAATCGTGTCGATGCCAACCAGTCTTTACTACTCTGCAGCCAAAAGCGAGGGGTTTCGGTACGTCGGAGACAGCCAACTTCATCGCATCGAAAACAGCACTGCTCTGCAGACGCTCTACCGACTAGACATCGCTGGTGGAGGCCCAACCTCATCCTATGAATACATGTACGGTTCCGATGACGTGTACCGCCGGTGGAATATTGCCGATGACGTGTACTTGGATAGTTTAAGCAAAACAATCAGCGAGCTAACATACAACGATACAATCCAGCTCAAATTTGTTAAAATTCCTGAGTACTCAGCACCAGAATATGTTTACCTTACAGGCCGGTCCCTGGGGATGGAGAAATACATCAACAAGAGCTACAATCTCACCTGGAGATTTCCCGTAGATTCTTCATTCCATTACCTTGTGAGGCTGCATTTCTGTGAGCTTGAACCAAGTTTTACCCAGTCCGGGGACAGAAAGTTTCAAATCAATATAAACAGCCAGACAGTTGAAGAAGGAGCAGACATAATCCAATGGAGTGGTGGAAATGGGATTCCAGTTTACAGAGACTACGTAGTGTACATGTCTGGCCCTGAAAATGAAAAGAACGTAGATCTCTCTCTCACTCTTGCAGCAGATGAGAAGAGCTCTTATAATGATGCAATCCTGAATGGGCTTGAAATCTTTAAACTACTAGACTCAAATGTCTCATATGACGCCCGACGCAATCCTGATTCACTGCAACTGAAAAGACCACAAAAGTCGAGTGAGTCAACTCCTACGGTTGCCATTGTCGCTGGTGTCATTTCCAGCATAATTGTGTTCTCGGTGATCGGATTCTTGGTCTTCAGGCGAGGATGGAAAGCTAAGGACTCAAGATCCAGCCACAGGACGAAGAAGTCAATGAAGGTCTATAAGTCATCTTTACCGACTTACTGTTGTCATTTTTCACTGCCAGAGATCAAAGTCGCCACTGAAAACTTCAATGCTACTTCTATTATTGGAGTTGGAGGGTTTGGCAATGTCTACAAAGGACGGATTGATAGCGAGGCAACCCTTGTTGCAATCAAGAGGCTAAAACCGGATTCATCACAGGGTGCTCATGAGTTTAAAACAGAAATTGAATTGCTCTCCCAAATCTGTCATCGCCATTTGGTGTCTTTGATTGGATATTGTAATGATAAAGGTGAGATGATCTTAGTGTATGATTACATGGCACGTGGGACACTCAGGGACCATCTCTACAACACTGAGAACCCACCTCTTCCTTGGAAAGAACGGCTACAGATTTGTATTGGCGCAGCACGAGGGTTGCAGTACCTTCATAGTGGTGCCATGGGCACCATCATTCACCGTGACGTGAAGAGCACTAACATCTTATTGGATGAAAACTGGGTGGCCAAGGTTTCAGATTTTGGGCTGTCGAAAGGCACGAATCACATGTCCAAAACCCACATCAGCACTCTAGTGAAAGGCAGTTTCGGGTATCTGGACCCAGAATATTTCCGAACACAACATCTGACTCCTCAATCAGACGTCTACTCATTCGGTGTAGTGTTGTGTGAAGTTTTGTGTGCGAGGCCTGCTGTGATACATACAGAGGAAATTAGGCAAGCAAACCTGGCTGAGTGGGCCAAGAGCTGTCATCAGAACGGGGAACTTGATCAAATCATTGATCCAATTTTGAGAGGTAACATCACAGCCGAGTGCCTGAATAAGTTTACTGAGGTAGCTATCAGTTGTATGCATGATAATGGAACAGAACGGCCGTCCATGACTGATGTTGTTAGGGGGCTTGAGCTTGCCTTGCAGCTTCAGCAGAGTGCAGAAGGGAATATCAACTGCATTGAAAGAAATGTCGAAACTGAAACAAGCAGTAGTGAGAAAAGTTGTGCCACCAACGATTCCATTAAATGCATATCTGCGACAATTTTCTCTGAGATTAACAACCCAATTGGGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009723 GO:0009725 GO:0009741 GO:0009787 GO:0009788 GO:0009791 GO:0009856 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010118 GO:0010483 GO:0010646 GO:0010648 GO:0014070 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022622 GO:0023051 GO:0023057 GO:0030054 GO:0030308 GO:0030312 GO:0032501 GO:0032502 GO:0033993 GO:0036211 GO:0040008 GO:0042221 GO:0043170 GO:0043207 GO:0043412 GO:0043680 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044426 GO:0044462 GO:0044464 GO:0044703 GO:0044706 GO:0045926 GO:0046777 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051128 GO:0051703 GO:0051704 GO:0051707 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:0098542 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1905957 GO:1905958

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Malectin_like Pkinase_Tyr

Protein Analysis

865

Amino Acids

97.26

Weight (kDa)

6.0

Isoelectric Point (pI)

47.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Malectin_like	PF12819	88 - 441	3.8e-30	Malectin-like domain
Malectin	PF11721	265 - 402	6.5e-09	Malectin domain
Pkinase	PF00069	540 - 807	9.7e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	542 - 809	5.1e-49	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000121)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g46570 FvH4_6g46580 FvH4_6g46610 FvH4_6g46620 FvH4_6g46640 FvH4_6g46680
malus_domestica	MD03G1063600.v1.1 MD03G1063700.v1.1 MD09G1069300.v1.1 MD09G1069400.v1.1 MD11G1067200.v1.1 MD11G1070300.v1.1 MD11G1153300.v1.1 MD17G1060300.v1.1 MD17G1061900.v1.1 MD17G1062100.v1.1 MD17G1062300.v1.1 MD17G1062400.v1.1 MD17G1063000.v1.1 MD17G1063200.v1.1 MD17G1063600.v1.1
prunus_persica	Prupe.3G246200_v2.0.a1 Prupe.3G251000_v2.0.a1 Prupe.3G251100_v2.0.a1 Prupe.3G251200_v2.0.a1 Prupe.3G251600_v2.0.a1 Prupe.3G251700_v2.0.a1 Prupe.3G251800_v2.0.a1 Prupe.3G251900_v2.0.a1
pyrus_communis	pycom03g05070 pycom03g05100 pycom12433g00070 pycom17g06000 pycom17g06170 pycom17g06180 pycom17g06200 pycom17g06210 pycom17g06280 pycom17g06300 pycom17g06310 pycom17g06320 pycom17g06330 pycom17g06350 pycom17g06370 pycom17g06380 pycom17g06400
rosa_chinensis	RchiOBHm_Chr1g0321851 RchiOBHm_Chr1g0321911 RchiOBHm_Chr1g0323951 RchiOBHm_Chr1g0361941 RchiOBHm_Chr2g0164711 RchiOBHm_Chr2g0165301 RchiOBHm_Chr2g0165311 RchiOBHm_Chr2g0165321 RchiOBHm_Chr2g0165351 RchiOBHm_Chr2g0165431 RchiOBHm_Chr2g0165461 RchiOBHm_Chr2g0165491 RchiOBHm_Chr2g0165511 RchiOBHm_Chr2g0165521 RchiOBHm_Chr2g0165531 RchiOBHm_Chr3g0481631 RchiOBHm_Chr3g0481801 RchiOBHm_Chr3g0481811 RchiOBHm_Chr3g0481821 RchiOBHm_Chr3g0481831 RchiOBHm_Chr4g0400291 RchiOBHm_Chr4g0400371 RchiOBHm_Chr4g0400391 RchiOBHm_Chr6g0274711 RchiOBHm_Chr6g0274721 RchiOBHm_Chr7g0211011
rosa_laevigata	RLG00000002260 RLG00000002262 RLG00000002263 RLG00000003027 RLG00000003028 RLG00000009196 RLG00000009197 RLG00000012573 RLG00000020400 RLG00000021523 RLG00000021524 RLG00000021525 RLG00000021527 RLG00000021528 RLG00000021529 RLG00000021530 RLG00000021532 RLG00000021533 RLG00000021534 RLG00000021535 RLG00000021537 RLG00000023384 RLG00000027681 RLG00000027682 RLG00000027685 RLG00000030240 RLG00000030401
rosa_multiflora	Rmu_co7998078.1_g000001 Rmu_co8009032.1_g000001 Rmu_co8326071.1_g000001 Rmu_co8333709.1_g000001 Rmu_co8362007.1_g000001 Rmu_co8369261.1_g000001 Rmu_co8391219.1_g000001 Rmu_co8456951.1_g000001 Rmu_co8461523.1_g000001 Rmu_sc0000427.1_g000009 Rmu_sc0000427.1_g000032 Rmu_sc0000427.1_g000033 Rmu_sc0000521.1_g000005 Rmu_sc0000521.1_g000008 Rmu_sc0000521.1_g000009 Rmu_sc0000655.1_g000016 Rmu_sc0000655.1_g000017 Rmu_sc0000655.1_g000018 Rmu_sc0000675.1_g000003 Rmu_sc0000795.1_g000018 Rmu_sc0000795.1_g000083 Rmu_sc0001306.1_g000042 Rmu_sc0001306.1_g000043 Rmu_sc0001873.1_g000001 Rmu_sc0001873.1_g000010 Rmu_sc0002529.1_g000002 Rmu_sc0002529.1_g000004 Rmu_sc0003410.1_g000004 Rmu_sc0003909.1_g000010 Rmu_sc0004097.1_g000001 Rmu_sc0005425.1_g000003 Rmu_sc0005425.1_g000004 Rmu_sc0005425.1_g000010 Rmu_sc0005425.1_g000012 Rmu_sc0005425.1_g000017 Rmu_sc0006223.1_g000014 Rmu_sc0006399.1_g000007 Rmu_sc0010324.1_g000001 Rmu_sc0010409.1_g000005 Rmu_sc0013532.1_g000005 Rmu_sc0016601.1_g000002 Rmu_sc0016601.1_g000003 Rmu_sc0016601.1_g000006 Rmu_sc0017773.1_g000001 Rmu_sc0017773.1_g000002 Rmu_sc0019514.1_g000002 Rmu_sc0021575.1_g000001 Rmu_sc0023720.1_g000002 Rmu_sc0030898.1_g000001 Rmu_sc0033982.1_g000001 Rmu_sc0034651.1_g000001 Rmu_sc0038482.1_g000001 Rmu_sc0038644.1_g000001 Rmu_sc0038644.1_g000003 Rmu_sc0039243.1_g000001 Rmu_sc0042715.1_g000001 Rmu_ssc0000124.1_g000003
rosa_roxburghii	Rroxscaffold_2G00085740 Rroxscaffold_2G00085760 Rroxscaffold_2G00085780 Rroxscaffold_2G00085800 Rroxscaffold_2G00085830 Rroxscaffold_2G00085850 Rroxscaffold_2G00085860 Rroxscaffold_4G00294610 Rroxscaffold_4G00326070 Rroxscaffold_4G00326080 Rroxscaffold_4G00327420 Rroxscaffold_4G00327430 Rroxscaffold_4G00327460 Rroxscaffold_4G00327470 Rroxscaffold_5G00345350 Rroxscaffold_6G00399890 Rroxscaffold_6G00400250 Rroxscaffold_7G00194000 Rroxscaffold_7G00194020 Rroxscaffold_7G00194050
rosa_rugosa	Rorug01G0034300 Rorug01G0034500 Rorug01G0040700 Rorug01G0064300 Rorug02G0513700 Rorug02G0513800 Rorug02G0513900 Rorug02G0514000 Rorug02G0514000 Rorug02G0514100 Rorug02G0514300 Rorug02G0514400 Rorug02G0514700 Rorug02G0515000 Rorug02G0515100 Rorug02G0515300 Rorug02G0515500 Rorug02G0515600 Rorug02G0515800 Rorug02G0515900 Rorug02G0516000 Rorug03G0193200 Rorug04G0024600 Rorug06G0083100 Rorug06G0085200 Rorug07G0188600
rosa_samantha	Rh1CG234000 Rh2DG603700 Rh2DG603800 Rh2DG603900 Rh6BG203000
rosa_wichuraiana	Rw0G010290 Rw1G004240 Rw1G005000 Rw1G026960 Rw2G048310 Rw2G048320 Rw2G048330 Rw2G048350 Rw2G048360 Rw2G048380 Rw2G048430 Rw2G048460 Rw2G048500 Rw2G048510 Rw2G048520 Rw2G048530 Rw3G021810 Rw4G008270 Rw6G017320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	255, 1287
AasI	GACNNNNNNGTC	2 cut(s)	1337, 1531
AatII	GACGTC	1 cut(s)	2172
AccB1I	GGYRCC	2 cut(s)	1964, 1973
AccI	GTMKAC	2 cut(s)	1659, 2172
AccII	CGCG	1 cut(s)	361
AciI	CCGC	5 cut(s)	246, 361, 402, 515, 832
AclI	AACGTT	1 cut(s)	561
AclWI	GGATC	2 cut(s)	1506, 2303
AcoI	YGGCCR	5 cut(s)	129, 304, 406, 2031, 2399
AcsI	RAATTY	5 cut(s)	290, 377, 548, 920, 930
AcuI	CTGAAG	2 cut(s)	1465, 2432
AcvI	CACGTG	1 cut(s)	1853
AcyI	GRCGYC	2 cut(s)	1350, 2169
AdeI	CACNNNGTG	1 cut(s)	1730
AfiI	CCNNNNNNNGG	6 cut(s)	127, 979, 985, 1101, 1102, 1521
AflIII	ACRYGT	5 cut(s)	807, 825, 855, 1215, 2073
AjiI	CACGTC	3 cut(s)	826, 856, 1993
AjnI	CCWGG	3 cut(s)	978, 1019, 2253
AjuI	GAANNNNNNNTTGG	2 cut(s)	458, 490
AleI	CACNNNNGTG	2 cut(s)	158, 2099
Alw21I	GWGCWC	3 cut(s)	1283, 1735, 2003
Alw26I	GTCTC	3 cut(s)	705, 1194, 1345
AlwI	GGATC	2 cut(s)	1506, 2303
ApeKI	GCWGC	5 cut(s)	680, 1063, 1265, 1934, 2443
ApoI	RAATTY	5 cut(s)	290, 377, 548, 920, 930
ArsI	GACNNNNNNTTYG	2 cut(s)	2060, 2092
AspLEI	GCGC	4 cut(s)	188, 239, 361, 1934
AspS9I	GGNCC	6 cut(s)	785, 975, 1223, 1867, 2125, 2265
AsuC2I	CCSGG	1 cut(s)	1102
AsuHPI	GGTGA	8 cut(s)	109, 163, 391, 503, 1009, 1474, 1832, 1976
AsuII	TTCGAA	1 cut(s)	287
AvaII	GGWCC	3 cut(s)	975, 1867, 2125
BaeGI	GKGCMC	1 cut(s)	1976
BalI	TGGCCA	2 cut(s)	306, 2033
BanI	GGYRCC	2 cut(s)	1964, 1973
BanII	GRGCYC	1 cut(s)	1283
BauI	CACGAG	1 cut(s)	1938
BbrPI	CACGTG	1 cut(s)	1853
BbsI	GAAGAC	1 cut(s)	1471
Bbv12I	GWGCWC	3 cut(s)	1283, 1735, 2003
BbvI	GCAGC	5 cut(s)	692, 1050, 1277, 1946, 2455
BccI	CCATC	5 cut(s)	488, 979, 1485, 1878, 1985
BceAI	ACGGC	3 cut(s)	1928, 2386, 2414
BciT130I	CCWGG	3 cut(s)	980, 1021, 2255
BclI	TGATCA	1 cut(s)	2296
BcnI	CCSGG	1 cut(s)	1102
BcoDI	GTCTC	3 cut(s)	705, 1194, 1345
BfaI	CTAG	3 cut(s)	767, 1328, 2099
BfmI	CTRYAG	3 cut(s)	678, 749, 1916
BfoI	RGCGCY	2 cut(s)	189, 240
BglII	AGATCT	1 cut(s)	1246
BisI	GCNGC	7 cut(s)	247, 362, 681, 1064, 1266, 1935, 2444
BlsI	GCNGC	7 cut(s)	248, 363, 682, 1065, 1267, 1936, 2445
BmcAI	AGTACT	1 cut(s)	941
Bme1390I	CCNGG	4 cut(s)	980, 1021, 1102, 2255
Bme18I	GGWCC	3 cut(s)	975, 1867, 2125
BmgBI	CACGTC	3 cut(s)	826, 856, 1993
BmgT120I	GGNCC	6 cut(s)	785, 975, 1223, 1867, 2125, 2265
BmiI	GGNNCC	6 cut(s)	817, 977, 1868, 1966, 1975, 2127
BmrFI	CCNGG	4 cut(s)	980, 1021, 1102, 2255
BmrI	ACTGGG	2 cut(s)	1090, 2035
BmsI	GCATC	6 cut(s)	152, 508, 607, 645, 738, 1282
BmuI	ACTGGG	2 cut(s)	1090, 2035
BpiI	GAAGAC	1 cut(s)	1471
BplI	GAGNNNNNCTC	2 cut(s)	1265, 1297
BpmI	CTGGAG	1 cut(s)	1042
Bpu10I	CCTNAGC	1 cut(s)	1500
Bpu14I	TTCGAA	1 cut(s)	287
BpuEI	CTTGAG	2 cut(s)	1492, 2450
BpuMI	CCSGG	1 cut(s)	1102
BsaAI	YACGTR	3 cut(s)	150, 1207, 1853
BsaHI	GRCGYC	2 cut(s)	1350, 2169
BsaJI	CCNNGG	6 cut(s)	978, 979, 1101, 1901, 1968, 2034
BsaWI	WCCGGW	2 cut(s)	157, 1714
BsaXI	ACNNNNNCTCC	6 cut(s)	12, 42, 139, 169, 521, 551
Bsc4I	CCNNNNNNNGG	6 cut(s)	127, 979, 985, 1101, 1102, 1521
Bse118I	RCCGGY	4 cut(s)	404, 488, 834, 972
Bse1I	ACTGG	5 cut(s)	238, 663, 1096, 1190, 2030
Bse3DI	GCAATG	1 cut(s)	1660
BseBI	CCWGG	3 cut(s)	980, 1021, 2255
BseDI	CCNNGG	6 cut(s)	978, 979, 1101, 1901, 1968, 2034
BseGI	GGATG	5 cut(s)	523, 794, 990, 1496, 2023
BseLI	CCNNNNNNNGG	6 cut(s)	127, 979, 985, 1101, 1102, 1521
BseMI	GCAATG	1 cut(s)	1660
BseMII	CTCAG	8 cut(s)	218, 927, 957, 1875, 1876, 2250, 2349, 2562
BseNI	ACTGG	5 cut(s)	238, 663, 1096, 1190, 2030
BseRI	GAGGAG	2 cut(s)	545, 2148
BseSI	GKGCMC	1 cut(s)	1976
BseX3I	CGGCCG	1 cut(s)	2399
BseXI	GCAGC	5 cut(s)	692, 1050, 1277, 1946, 2455
BsgI	GTGCAG	1 cut(s)	2478
Bsh1236I	CGCG	1 cut(s)	361
Bsh1285I	CGRYCG	1 cut(s)	2402
BshNI	GGYRCC	2 cut(s)	1964, 1973
BsiEI	CGRYCG	1 cut(s)	2402
BsiHKAI	GWGCWC	3 cut(s)	1283, 1735, 2003
BsiSI	CCGG	8 cut(s)	132, 158, 405, 489, 835, 973, 1101, 1715
BslFI	GGGAC	5 cut(s)	208, 961, 1118, 1870, 1880
BslI	CCNNNNNNNGG	6 cut(s)	127, 979, 985, 1101, 1102, 1521
BsmAI	GTCTC	3 cut(s)	705, 1194, 1345
BsmFI	GGGAC	5 cut(s)	208, 961, 1118, 1870, 1880
Bsp119I	TTCGAA	1 cut(s)	287
Bsp1286I	GDGCHC	4 cut(s)	1283, 1735, 1976, 2003
Bsp1407I	TGTACA	1 cut(s)	1212
Bsp143I	GATC	7 cut(s)	1246, 1464, 1511, 1593, 1827, 2296, 2308
Bsp19I	CCATGG	1 cut(s)	1968
BspACI	CCGC	5 cut(s)	246, 361, 402, 515, 832
BspCNI	CTCAG	8 cut(s)	217, 928, 956, 1875, 1876, 2251, 2350, 2563
BspFNI	CGCG	1 cut(s)	361
BspHI	TCATGA	1 cut(s)	1735
BspLI	GGNNCC	6 cut(s)	817, 977, 1868, 1966, 1975, 2127
BspMAI	CTGCAG	2 cut(s)	682, 753
BspPI	GGATC	2 cut(s)	1506, 2303
BspQI	GCTCTTC	2 cut(s)	1271, 1991
BspT104I	TTCGAA	1 cut(s)	287
BspT107I	GGYRCC	2 cut(s)	1964, 1973
BsrDI	GCAATG	1 cut(s)	1660
BsrFI	RCCGGY	4 cut(s)	404, 488, 834, 972
BsrGI	TGTACA	1 cut(s)	1212
BsrI	ACTGG	5 cut(s)	238, 663, 1096, 1190, 2030
BssAI	RCCGGY	4 cut(s)	404, 488, 834, 972
BssECI	CCNNGG	6 cut(s)	978, 979, 1101, 1901, 1968, 2034
BssMI	GATC	7 cut(s)	1246, 1464, 1511, 1593, 1827, 2296, 2308
BssNI	GRCGYC	2 cut(s)	1350, 2169
BssSI	CACGAG	1 cut(s)	1938
BssT1I	CCWWGG	3 cut(s)	1901, 1968, 2034
Bst2BI	CACGAG	1 cut(s)	1938
Bst2UI	CCWGG	3 cut(s)	980, 1021, 2255
Bst4CI	ACNGT	9 cut(s)	175, 199, 423, 638, 815, 1141, 1417, 1571, 1988
Bst6I	CTCTTC	3 cut(s)	1271, 1902, 1991
BstACI	GRCGYC	2 cut(s)	1350, 2169
BstAUI	TGTACA	1 cut(s)	1212
BstBAI	YACGTR	3 cut(s)	150, 1207, 1853
BstBI	TTCGAA	1 cut(s)	287
BstC8I	GCNNGC	6 cut(s)	406, 472, 890, 2217, 2247, 2436
BstDSI	CCRYGG	1 cut(s)	1968
BstF5I	GGATG	5 cut(s)	523, 794, 990, 1496, 2023
BstFNI	CGCG	1 cut(s)	361
BstH2I	RGCGCY	2 cut(s)	189, 240
BstHHI	GCGC	4 cut(s)	188, 239, 361, 1934
BstKTI	GATC	7 cut(s)	1249, 1467, 1514, 1596, 1830, 2299, 2311
BstMAI	GTCTC	3 cut(s)	705, 1194, 1345
BstMBI	GATC	7 cut(s)	1246, 1464, 1511, 1593, 1827, 2296, 2308
BstMCI	CGRYCG	1 cut(s)	2402
BstMWI	GCNNNNNNNGC	8 cut(s)	185, 243, 1427, 2216, 2255, 2264, 2440, 2449
BstNI	CCWGG	3 cut(s)	980, 1021, 2255
BstNSI	RCATGY	3 cut(s)	811, 1219, 2077
BstSCI	CCNGG	4 cut(s)	978, 1019, 1100, 2253
BstSFI	CTRYAG	3 cut(s)	678, 749, 1916
BstSLI	GKGCMC	1 cut(s)	1976
BstSNI	TACGTA	2 cut(s)	150, 1207
BstUI	CGCG	1 cut(s)	361
BstV1I	GCAGC	5 cut(s)	692, 1050, 1277, 1946, 2455
BstV2I	GAAGAC	1 cut(s)	1471
BstX2I	RGATCY	2 cut(s)	1246, 1511
BstXI	CCANNNNNNTGG	1 cut(s)	1172
BstYI	RGATCY	2 cut(s)	1246, 1511
BstZI	CGGCCG	1 cut(s)	2399
BtgI	CCRYGG	1 cut(s)	1968
BtgZI	GCGATG	3 cut(s)	710, 757, 1763
BtrI	CACGTC	3 cut(s)	826, 856, 1993
BtsCI	GGATG	5 cut(s)	523, 794, 990, 1496, 2023
BtsI	GCAGTG	4 cut(s)	510, 741, 1370, 1583
BtsIMutI	CAGTG	7 cut(s)	231, 510, 741, 1370, 1583, 1605, 1881
Cac8I	GCNNGC	6 cut(s)	406, 472, 890, 2217, 2247, 2436
CciI	TCATGA	1 cut(s)	1735
CfoI	GCGC	4 cut(s)	188, 239, 361, 1934
Cfr10I	RCCGGY	4 cut(s)	404, 488, 834, 972
Cfr13I	GGNCC	6 cut(s)	785, 975, 1223, 1867, 2125, 2265
CseI	GACGC	3 cut(s)	763, 1358, 1365
CviAII	CATG	8 cut(s)	808, 1216, 1736, 1846, 1969, 2074, 2381, 2407
DpnI	GATC	7 cut(s)	1248, 1466, 1513, 1595, 1829, 2298, 2310
DpnII	GATC	7 cut(s)	1246, 1464, 1511, 1593, 1827, 2296, 2308
DraI	TTTAAA	2 cut(s)	1321, 1744
DraIII	CACNNNGTG	1 cut(s)	1730
DrdI	GACNNNNNNGTC	2 cut(s)	1337, 1531
DseDI	GACNNNNNNGTC	2 cut(s)	1337, 1531
EaeI	YGGCCR	5 cut(s)	129, 304, 406, 2031, 2399
EagI	CGGCCG	1 cut(s)	2399
Eam1104I	CTCTTC	3 cut(s)	1271, 1902, 1991
EarI	CTCTTC	3 cut(s)	1271, 1902, 1991
Ecl136II	GAGCTC	1 cut(s)	1281
EclXI	CGGCCG	1 cut(s)	2399
Eco105I	TACGTA	2 cut(s)	150, 1207
Eco130I	CCWWGG	3 cut(s)	1901, 1968, 2034
Eco147I	AGGCCT	1 cut(s)	2215
Eco24I	GRGCYC	1 cut(s)	1283
Eco32I	GATATC	1 cut(s)	280
Eco47I	GGWCC	3 cut(s)	975, 1867, 2125
Eco52I	CGGCCG	1 cut(s)	2399
Eco53kI	GAGCTC	1 cut(s)	1281
Eco57I	CTGAAG	2 cut(s)	1465, 2432
Eco72I	CACGTG	1 cut(s)	1853
EcoICRI	GAGCTC	1 cut(s)	1281
EcoRI	GAATTC	1 cut(s)	548
EcoRII	CCWGG	3 cut(s)	978, 1019, 2253
EcoRV	GATATC	1 cut(s)	280
EcoT14I	CCWWGG	3 cut(s)	1901, 1968, 2034
EcoT22I	ATGCAT	2 cut(s)	2382, 2552
EcoT38I	GRGCYC	1 cut(s)	1283
ErhI	CCWWGG	3 cut(s)	1901, 1968, 2034
FaeI	CATG	8 cut(s)	811, 1219, 1739, 1849, 1972, 2077, 2384, 2410
FaqI	GGGAC	5 cut(s)	208, 961, 1118, 1870, 1880
FatI	CATG	8 cut(s)	807, 1215, 1735, 1845, 1968, 2073, 2380, 2406
FauNDI	CATATG	1 cut(s)	1345
FbaI	TGATCA	1 cut(s)	2296
FblI	GTMKAC	2 cut(s)	1659, 2172
Fnu4HI	GCNGC	7 cut(s)	247, 362, 681, 1064, 1266, 1935, 2444
FokI	GGATG	5 cut(s)	530, 781, 997, 1503, 2030
FriOI	GRGCYC	1 cut(s)	1283
Fsp4HI	GCNGC	7 cut(s)	247, 362, 681, 1064, 1266, 1935, 2444
FspBI	CTAG	3 cut(s)	767, 1328, 2099
GlaI	GCGC	4 cut(s)	187, 238, 360, 1933
GluI	GCNGC	7 cut(s)	247, 362, 681, 1064, 1266, 1935, 2444
GsuI	CTGGAG	1 cut(s)	1042
HaeII	RGCGCY	2 cut(s)	189, 240
HapII	CCGG	8 cut(s)	132, 158, 405, 489, 835, 973, 1101, 1715
HgaI	GACGC	3 cut(s)	763, 1358, 1365
HhaI	GCGC	4 cut(s)	188, 239, 361, 1934
Hin1I	GRCGYC	2 cut(s)	1350, 2169
Hin1II	CATG	8 cut(s)	811, 1219, 1739, 1849, 1972, 2077, 2384, 2410
Hin6I	GCGC	4 cut(s)	186, 237, 359, 1932
HinP1I	GCGC	4 cut(s)	186, 237, 359, 1932
HincII	GTYRAC	3 cut(s)	328, 565, 1407
HindII	GTYRAC	3 cut(s)	328, 565, 1407
HpaII	CCGG	8 cut(s)	132, 158, 405, 489, 835, 973, 1101, 1715
HphI	GGTGA	8 cut(s)	109, 163, 391, 503, 1009, 1474, 1832, 1976
Hpy188III	TCNNGA	9 cut(s)	15, 506, 935, 1300, 1364, 1509, 1702, 1736, 2123
Hpy99I	CGWCG	2 cut(s)	710, 1359
HpyAV	CCTTC	5 cut(s)	512, 1142, 1534, 1964, 2456
HpyCH4III	ACNGT	9 cut(s)	175, 199, 423, 638, 815, 1141, 1417, 1571, 1988
HpyF10VI	GCNNNNNNNGC	8 cut(s)	185, 243, 1427, 2216, 2255, 2264, 2440, 2449
Hsp92I	GRCGYC	2 cut(s)	1350, 2169
Hsp92II	CATG	8 cut(s)	811, 1219, 1739, 1849, 1972, 2077, 2384, 2410
HspAI	GCGC	4 cut(s)	186, 237, 359, 1932
KroI	GCCGGC	1 cut(s)	404
KroNI	GCCGGC	1 cut(s)	406
Ksp22I	TGATCA	1 cut(s)	2296
Kzo9I	GATC	7 cut(s)	1246, 1464, 1511, 1593, 1827, 2296, 2308
LguI	GCTCTTC	2 cut(s)	1271, 1991
LmnI	GCTCC	1 cut(s)	1151
Lsp1109I	GCAGC	5 cut(s)	692, 1050, 1277, 1946, 2455
LweI	GCATC	6 cut(s)	152, 508, 607, 645, 738, 1282
MaeI	CTAG	3 cut(s)	767, 1328, 2099
MaeIII	GTNAC	5 cut(s)	115, 199, 491, 1988, 2324
MalI	GATC	7 cut(s)	1248, 1466, 1513, 1595, 1829, 2298, 2310
MboI	GATC	7 cut(s)	1246, 1464, 1511, 1593, 1827, 2296, 2308
MboII	GAAGA	8 cut(s)	581, 1032, 1157, 1288, 1471, 1540, 1889, 2008
MfeI	CAATTG	1 cut(s)	2586
MflI	RGATCY	2 cut(s)	1246, 1511
MhlI	GDGCHC	4 cut(s)	1283, 1735, 1976, 2003
MlsI	TGGCCA	2 cut(s)	306, 2033
MluNI	TGGCCA	2 cut(s)	306, 2033
MlyI	GAGTC	5 cut(s)	586, 1325, 1412, 1499, 2149
MmeI	TCCRAC	2 cut(s)	688, 1621
Mox20I	TGGCCA	2 cut(s)	306, 2033
Mph1103I	ATGCAT	2 cut(s)	2382, 2552
MroNI	GCCGGC	1 cut(s)	404
MscI	TGGCCA	2 cut(s)	306, 2033
MseI	TTAA	7 cut(s)	260, 872, 927, 1320, 1743, 2544, 2577
MslI	CAYNNNNRTG	7 cut(s)	158, 270, 1071, 1451, 1962, 2099, 2385
Msp20I	TGGCCA	2 cut(s)	306, 2033
MspI	CCGG	8 cut(s)	132, 158, 405, 489, 835, 973, 1101, 1715
MspR9I	CCNGG	4 cut(s)	980, 1021, 1102, 2255
MunI	CAATTG	1 cut(s)	2586
MvaI	CCWGG	3 cut(s)	980, 1021, 2255
MvnI	CGCG	1 cut(s)	361
MwoI	GCNNNNNNNGC	8 cut(s)	185, 243, 1427, 2216, 2255, 2264, 2440, 2449
NaeI	GCCGGC	1 cut(s)	406
NciI	CCSGG	1 cut(s)	1102
NcoI	CCATGG	1 cut(s)	1968
NdeI	CATATG	1 cut(s)	1345
NdeII	GATC	7 cut(s)	1246, 1464, 1511, 1593, 1827, 2296, 2308
NgoMIV	GCCGGC	1 cut(s)	404
NlaIII	CATG	8 cut(s)	811, 1219, 1739, 1849, 1972, 2077, 2384, 2410
NlaIV	GGNNCC	6 cut(s)	817, 977, 1868, 1966, 1975, 2127
NmeAIII	GCCGAG	1 cut(s)	2362
NmuCI	GTSAC	4 cut(s)	115, 199, 491, 1988
NsiI	ATGCAT	2 cut(s)	2382, 2552
NspI	RCATGY	3 cut(s)	811, 1219, 2077
NspV	TTCGAA	1 cut(s)	287
OliI	CACNNNNGTG	2 cut(s)	158, 2099
PagI	TCATGA	1 cut(s)	1735
PasI	CCCWGGG	1 cut(s)	979
PceI	AGGCCT	1 cut(s)	2215
PciI	ACATGT	3 cut(s)	807, 1215, 2073
PciSI	GCTCTTC	2 cut(s)	1271, 1991
PcsI	WCGNNNNNNNCGW	1 cut(s)	2063
PdiI	GCCGGC	1 cut(s)	406
PkrI	GCNGC	7 cut(s)	248, 363, 682, 1065, 1267, 1936, 2445
PleI	GAGTC	5 cut(s)	585, 1325, 1411, 1499, 2149
PmaCI	CACGTG	1 cut(s)	1853
PmlI	CACGTG	1 cut(s)	1853
PpsI	GAGTC	5 cut(s)	585, 1325, 1411, 1499, 2149
Ppu21I	YACGTR	3 cut(s)	150, 1207, 1853
PscI	ACATGT	3 cut(s)	807, 1215, 2073
PsiI	TTATAA	2 cut(s)	255, 1287
Psp124BI	GAGCTC	1 cut(s)	1283
Psp1406I	AACGTT	1 cut(s)	561
Psp6I	CCWGG	3 cut(s)	978, 1019, 2253
PspCI	CACGTG	1 cut(s)	1853
PspGI	CCWGG	3 cut(s)	978, 1019, 2253
PspN4I	GGNNCC	6 cut(s)	817, 977, 1868, 1966, 1975, 2127
PspPI	GGNCC	6 cut(s)	785, 975, 1223, 1867, 2125, 2265
PstI	CTGCAG	2 cut(s)	682, 753
PsuI	RGATCY	2 cut(s)	1246, 1511
RseI	CAYNNNNRTG	7 cut(s)	158, 270, 1071, 1451, 1962, 2099, 2385
SacI	GAGCTC	1 cut(s)	1283
SapI	GCTCTTC	2 cut(s)	1271, 1991
SaqAI	TTAA	7 cut(s)	260, 872, 927, 1320, 1743, 2544, 2577
SatI	GCNGC	7 cut(s)	247, 362, 681, 1064, 1266, 1935, 2444
Sau3AI	GATC	7 cut(s)	1246, 1464, 1511, 1593, 1827, 2296, 2308
Sau96I	GGNCC	6 cut(s)	785, 975, 1223, 1867, 2125, 2265
ScaI	AGTACT	1 cut(s)	941
SchI	GAGTC	5 cut(s)	586, 1325, 1412, 1499, 2149
ScrFI	CCNGG	4 cut(s)	980, 1021, 1102, 2255
SduI	GDGCHC	4 cut(s)	1283, 1735, 1976, 2003
SfaNI	GCATC	6 cut(s)	152, 508, 607, 645, 738, 1282
SfcI	CTRYAG	3 cut(s)	678, 749, 1916
SfuI	TTCGAA	1 cut(s)	287
SgrAI	CRCCGGYG	1 cut(s)	834
SinI	GGWCC	3 cut(s)	975, 1867, 2125
SmiMI	CAYNNNNRTG	7 cut(s)	158, 270, 1071, 1451, 1962, 2099, 2385
SmlI	CTYRAG	2 cut(s)	1507, 2429
SmoI	CTYRAG	2 cut(s)	1507, 2429
SnaBI	TACGTA	2 cut(s)	150, 1207
SseBI	AGGCCT	1 cut(s)	2215
SsiI	CCGC	5 cut(s)	246, 361, 402, 515, 832
SspI	AATATT	2 cut(s)	844, 2135
SspMI	CTAG	3 cut(s)	767, 1328, 2099
SstI	GAGCTC	1 cut(s)	1283
StuI	AGGCCT	1 cut(s)	2215
StyD4I	CCNGG	4 cut(s)	978, 1019, 1100, 2253
StyI	CCWWGG	3 cut(s)	1901, 1968, 2034
TaaI	ACNGT	9 cut(s)	175, 199, 423, 638, 815, 1141, 1417, 1571, 1988
TaqI	TCGA	7 cut(s)	287, 335, 653, 732, 1397, 2057, 2493
TatI	WGTACW	4 cut(s)	426, 858, 939, 1212
TauI	GCSGC	2 cut(s)	249, 364
Tru1I	TTAA	7 cut(s)	260, 872, 927, 1320, 1743, 2544, 2577
Tru9I	TTAA	7 cut(s)	260, 872, 927, 1320, 1743, 2544, 2577
TscAI	CASTG	7 cut(s)	238, 517, 748, 1377, 1590, 1612, 1888
TseFI	GTSAC	4 cut(s)	115, 199, 491, 1988
TseI	GCWGC	5 cut(s)	680, 1063, 1265, 1934, 2443
Tsp45I	GTSAC	4 cut(s)	115, 199, 491, 1988
TspGWI	ACGGA	1 cut(s)	1684
TspRI	CASTG	7 cut(s)	238, 517, 748, 1377, 1590, 1612, 1888
VpaK11BI	GGWCC	3 cut(s)	975, 1867, 2125
XapI	RAATTY	5 cut(s)	290, 377, 548, 920, 930
XceI	RCATGY	3 cut(s)	811, 1219, 2077
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1172, 1899
XmiI	GTMKAC	2 cut(s)	1659, 2172
XspI	CTAG	3 cut(s)	767, 1328, 2099
ZraI	GACGTC	1 cut(s)	2170
ZrmI	AGTACT	1 cut(s)	941
Zsp2I	ATGCAT	2 cut(s)	2382, 2552

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.