Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0000121

Physical Location & Seq

Genomic Coordinates Reverse (-)

5110624 .. 5113128

Gene Structure 2505 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1063000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2505 bp

ATGAAACCCAATCTTGCTCATCTGTTCCTTACCCTGTTTCTGCAAATCATAACCCTACACGTGGCCGGAGACTCACCTTCTGTCTACAAACCGGTCGATGACATCACCCTACAGTGCGGCCTTTCCGGCGACCAAGTCAACCAACAGGACGGACGAACTTGGAGTGGCGATATCAACTCAAAATTCTCCCCCTCCCAAGCAGCTGGCAGCTCATCCATATTCAAAGCAGCAACCCCGATCTCCTCTTCCAGCCAAGTGCCTTACACCGCAGCTCGGCTCTCTCGCTCCGAATTTACGTACACATTTCCACTCACCAGCGGCCAAAAGTTCATCCGCTTGTACTTCTACCCCACCTCCTACGCCGCCGAGTTCGACCGATCTAAATCCCTCTTCTCAGTCGAAGCCGGCGGCTTTACCCTTCTCCGCGACTTCAACGCCTCCGTCACCGCCGACGCTTCCTGGTCGGAGACAGTATCCAGGGAGTTCTGTCTCAACATAGCATCCGAACAGAGCTTGAACATCACGTTCACTCCGAGCAGAGCAAGTCCAGATGCCTATGCCTTCATCAATGGAATCGAAATCGTGTCCATGCCCGACAATCTTTACTACACTCCACCACAAAGCGCAGATGGGCCTGTTTCCGTAGGCAGCATAAACCCGTATCCGATCGAAAATAACACCGCCATGGAGATGGTCTACCGGATCAACGTCGGCGGATCTCAGCTCTCGTTCAATCGGGACACCGGAATATACAGAAACTGGGATGGTATTCAAGTGGACTACAACTATTTGGACGATTTGAGTAAAAACTTTAGTGTTCTGCCGCAAAACAGTAGTATTCAGCCCGACTTTTCGAAAATTCCGGAGTACTCTGCTCCGGAAGAAGTTTACCGAACCGCTCGGTCGATGGGGAGGAACAAAACCATAAACAAGAGCTATAATCTCACCTGGGGGTTTCCGGTAGATTCTACGTTTTATTACTTTATTCGGCTTCATTTTTGTGAGTTCGAAGGTGCTGTTACATTGCCCAGGGACCGGCTGTTTCAAATCTTCATCGCCAATCAGATGGCGGATGTAATCACGTGGAGCCAAGGTTATGGGAAGCCAGTGTACAAAGACTACGTCGTTTTCGTGCCTGCCGGCGCCGGAAGTAAAAAGAAAGTTCATCTCTTTCTTGCACTGAAAGCCACCCCAAAAGATTGGTTGACGAATTACAACGATGCAATCTTGAACGGACTCGAGATCTTCAAGCTCAGTGATTCAAATAGGAATCTCGCCGGACCAAACCCCGTCCCGCCTCCCCTGCCAAATATAGCTCAGCCGGAGAGCCCCAAAGTTTCAGACAAGAAATCTATTCCTCTGCATGCGATCATCGCCGGTGTAGTTTCAGGTGTGTTTGTTTTGGCCTCTGTGTTTGGATTTTTGGTTTTCGGGAGAGGAAGAAAAGTCAAGGACACTGCCTCAGATGGGTCGTCATCTTTACCCCCACATTTGTGTCGTTGCTTTTCACTGTCGGAGATCAAAGCCGCCACCCAAAACTTCAGCCAGACTTTCATTGTTGGTGTTGGTGGCTTCGGTCACGTGTACAAAGGGCATGTCGACGGCGGGGCCACTCCCGTTGCTATCAAACGGCTGAAACCCGAGTCATCACAGGGAGCCCGTGAGTTCAAGACGGAAATCGAGCTGCTCTCACAACTCAGACACCGCCATTTGGTGTCTCTTATTGGGTATTGTACTGATAAAAATGAGATGATTTTGGTATACGATTACATGGCACGCGGGACCCTCGCCGACCATCTCTACCACACCGACAACCCACCTCTCTCCTGGGGACAACGGCTCCAAATTTGCATTGGAGCTGCACGAGGGTTGCGCTACCTTCACAGCGATGCCCAGGGCACTGTCATCCATCGTGACGTGAAGAGCACAAACATTTTATTGGATGAGAAATGGGCGGCCAAGGTTTCGGATTTCGGATTGTCGAAAGTGTGCACGACCACCATGTCCAAGATCCACATCAGCACGACCGTGAAAGGCAGTTTCGGGTATCTAGACCCGGAATACTACCGACGTCAACAACTAACGGTGAAGTCTGATGTGTACTCATTCGGTGTAGTGTTGTGTGAAGTATTGTGCGCAAGACCAGCTGTGGTGCATGCAGCGGAGACGAGGCAAATGAACTTGGCTGAGTGGACCAAGAGCTGCCATCGTGATGGGGAACTTGATCAAATCATTGATCCGAACATGAAGGGTAAGATTGAAACTGATTGTTTGAACAAGTTTGTGGAAATTGCTATGAGTTGCATCAATGATAGTGGGATTGAACGGCCGTCAATGGACGATGTTGTGAGGGGGCTTGAGTTTGCATTGCAACTACATCAGAATTGCGGTGACAGGAACGACTATGAAGCCTTCACTAGTGTGCCAGGTTGCGCTACGAATGAATCCGTTTTGTGTATATCTGAGACGATCTTCTCAGAAATCAACGATCTGAATGGGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009723 GO:0009725 GO:0009741 GO:0009787 GO:0009788 GO:0009791 GO:0009856 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010118 GO:0010483 GO:0010646 GO:0010648 GO:0014070 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022622 GO:0023051 GO:0023057 GO:0030054 GO:0030308 GO:0030312 GO:0032501 GO:0032502 GO:0033993 GO:0036211 GO:0040008 GO:0042221 GO:0043170 GO:0043207 GO:0043412 GO:0043680 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044426 GO:0044462 GO:0044464 GO:0044703 GO:0044706 GO:0045926 GO:0046777 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051128 GO:0051703 GO:0051704 GO:0051707 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:0098542 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1905957 GO:1905958

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Malectin_like Pkinase_Tyr

Protein Analysis

835

Amino Acids

92.42

Weight (kDa)

6.34

Isoelectric Point (pI)

40.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Malectin_like	PF12819	37 - 417	1.8e-31	Malectin-like domain
Pkinase	PF00069	519 - 783	1.6e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	520 - 785	1.3e-47	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000121)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g46570 FvH4_6g46580 FvH4_6g46610 FvH4_6g46620 FvH4_6g46640 FvH4_6g46680
malus_domestica	MD03G1063600.v1.1 MD03G1063700.v1.1 MD09G1069300.v1.1 MD09G1069400.v1.1 MD11G1067200.v1.1 MD11G1070300.v1.1 MD11G1153300.v1.1 MD17G1060300.v1.1 MD17G1061900.v1.1 MD17G1062100.v1.1 MD17G1062300.v1.1 MD17G1062400.v1.1 MD17G1063000.v1.1 MD17G1063200.v1.1 MD17G1063600.v1.1
prunus_persica	Prupe.3G246200_v2.0.a1 Prupe.3G251000_v2.0.a1 Prupe.3G251100_v2.0.a1 Prupe.3G251200_v2.0.a1 Prupe.3G251600_v2.0.a1 Prupe.3G251700_v2.0.a1 Prupe.3G251800_v2.0.a1 Prupe.3G251900_v2.0.a1
pyrus_communis	pycom03g05070 pycom03g05100 pycom12433g00070 pycom17g06000 pycom17g06170 pycom17g06180 pycom17g06200 pycom17g06210 pycom17g06280 pycom17g06300 pycom17g06310 pycom17g06320 pycom17g06330 pycom17g06350 pycom17g06370 pycom17g06380 pycom17g06400
rosa_chinensis	RchiOBHm_Chr1g0321851 RchiOBHm_Chr1g0321911 RchiOBHm_Chr1g0323951 RchiOBHm_Chr1g0361941 RchiOBHm_Chr2g0164711 RchiOBHm_Chr2g0165301 RchiOBHm_Chr2g0165311 RchiOBHm_Chr2g0165321 RchiOBHm_Chr2g0165351 RchiOBHm_Chr2g0165431 RchiOBHm_Chr2g0165461 RchiOBHm_Chr2g0165491 RchiOBHm_Chr2g0165511 RchiOBHm_Chr2g0165521 RchiOBHm_Chr2g0165531 RchiOBHm_Chr3g0481631 RchiOBHm_Chr3g0481801 RchiOBHm_Chr3g0481811 RchiOBHm_Chr3g0481821 RchiOBHm_Chr3g0481831 RchiOBHm_Chr4g0400291 RchiOBHm_Chr4g0400371 RchiOBHm_Chr4g0400391 RchiOBHm_Chr6g0274711 RchiOBHm_Chr6g0274721 RchiOBHm_Chr7g0211011
rosa_laevigata	RLG00000002260 RLG00000002262 RLG00000002263 RLG00000003027 RLG00000003028 RLG00000009196 RLG00000009197 RLG00000012573 RLG00000020400 RLG00000021523 RLG00000021524 RLG00000021525 RLG00000021527 RLG00000021528 RLG00000021529 RLG00000021530 RLG00000021532 RLG00000021533 RLG00000021534 RLG00000021535 RLG00000021537 RLG00000023384 RLG00000027681 RLG00000027682 RLG00000027685 RLG00000030240 RLG00000030401
rosa_multiflora	Rmu_co7998078.1_g000001 Rmu_co8009032.1_g000001 Rmu_co8326071.1_g000001 Rmu_co8333709.1_g000001 Rmu_co8362007.1_g000001 Rmu_co8369261.1_g000001 Rmu_co8391219.1_g000001 Rmu_co8456951.1_g000001 Rmu_co8461523.1_g000001 Rmu_sc0000427.1_g000009 Rmu_sc0000427.1_g000032 Rmu_sc0000427.1_g000033 Rmu_sc0000521.1_g000005 Rmu_sc0000521.1_g000008 Rmu_sc0000521.1_g000009 Rmu_sc0000655.1_g000016 Rmu_sc0000655.1_g000017 Rmu_sc0000655.1_g000018 Rmu_sc0000675.1_g000003 Rmu_sc0000795.1_g000018 Rmu_sc0000795.1_g000083 Rmu_sc0001306.1_g000042 Rmu_sc0001306.1_g000043 Rmu_sc0001873.1_g000001 Rmu_sc0001873.1_g000010 Rmu_sc0002529.1_g000002 Rmu_sc0002529.1_g000004 Rmu_sc0003410.1_g000004 Rmu_sc0003909.1_g000010 Rmu_sc0004097.1_g000001 Rmu_sc0005425.1_g000003 Rmu_sc0005425.1_g000004 Rmu_sc0005425.1_g000010 Rmu_sc0005425.1_g000012 Rmu_sc0005425.1_g000017 Rmu_sc0006223.1_g000014 Rmu_sc0006399.1_g000007 Rmu_sc0010324.1_g000001 Rmu_sc0010409.1_g000005 Rmu_sc0013532.1_g000005 Rmu_sc0016601.1_g000002 Rmu_sc0016601.1_g000003 Rmu_sc0016601.1_g000006 Rmu_sc0017773.1_g000001 Rmu_sc0017773.1_g000002 Rmu_sc0019514.1_g000002 Rmu_sc0021575.1_g000001 Rmu_sc0023720.1_g000002 Rmu_sc0030898.1_g000001 Rmu_sc0033982.1_g000001 Rmu_sc0034651.1_g000001 Rmu_sc0038482.1_g000001 Rmu_sc0038644.1_g000001 Rmu_sc0038644.1_g000003 Rmu_sc0039243.1_g000001 Rmu_sc0042715.1_g000001 Rmu_ssc0000124.1_g000003
rosa_roxburghii	Rroxscaffold_2G00085740 Rroxscaffold_2G00085760 Rroxscaffold_2G00085780 Rroxscaffold_2G00085800 Rroxscaffold_2G00085830 Rroxscaffold_2G00085850 Rroxscaffold_2G00085860 Rroxscaffold_4G00294610 Rroxscaffold_4G00326070 Rroxscaffold_4G00326080 Rroxscaffold_4G00327420 Rroxscaffold_4G00327430 Rroxscaffold_4G00327460 Rroxscaffold_4G00327470 Rroxscaffold_5G00345350 Rroxscaffold_6G00399890 Rroxscaffold_6G00400250 Rroxscaffold_7G00194000 Rroxscaffold_7G00194020 Rroxscaffold_7G00194050
rosa_rugosa	Rorug01G0034300 Rorug01G0034500 Rorug01G0040700 Rorug01G0064300 Rorug02G0513700 Rorug02G0513800 Rorug02G0513900 Rorug02G0514000 Rorug02G0514000 Rorug02G0514100 Rorug02G0514300 Rorug02G0514400 Rorug02G0514700 Rorug02G0515000 Rorug02G0515100 Rorug02G0515300 Rorug02G0515500 Rorug02G0515600 Rorug02G0515800 Rorug02G0515900 Rorug02G0516000 Rorug03G0193200 Rorug04G0024600 Rorug06G0083100 Rorug06G0085200 Rorug07G0188600
rosa_samantha	Rh1CG234000 Rh2DG603700 Rh2DG603800 Rh2DG603900 Rh6BG203000
rosa_wichuraiana	Rw0G010290 Rw1G004240 Rw1G005000 Rw1G026960 Rw2G048310 Rw2G048320 Rw2G048330 Rw2G048350 Rw2G048360 Rw2G048380 Rw2G048430 Rw2G048460 Rw2G048500 Rw2G048510 Rw2G048520 Rw2G048530 Rw3G021810 Rw4G008270 Rw6G017320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2002
AatII	GACGTC	1 cut(s)	2074
Acc16I	TGCGCA	1 cut(s)	2137
AccB1I	GGYRCC	1 cut(s)	1142
AccBSI	CCGCTC	1 cut(s)	899
AccI	GTMKAC	4 cut(s)	84, 696, 1599, 1761
AccII	CGCG	2 cut(s)	426, 1779
AccIII	TCCGGA	2 cut(s)	862, 877
AclWI	GGATC	4 cut(s)	710, 724, 2005, 2231
AcoI	YGGCCR	4 cut(s)	63, 319, 1956, 2327
AcsI	RAATTY	4 cut(s)	182, 290, 858, 1845
AcuI	CTGAAG	1 cut(s)	1525
AcvI	CACGTG	3 cut(s)	61, 1083, 1582
AcyI	GRCGYC	2 cut(s)	1143, 2071
AfaI	GTAC	7 cut(s)	299, 341, 869, 1112, 1586, 1735, 2102
AfiI	CCNNNNNNNGG	5 cut(s)	61, 273, 1035, 1467, 1711
AflIII	ACRYGT	2 cut(s)	58, 1581
AgeI	ACCGGT	1 cut(s)	91
AhlI	ACTAGT	1 cut(s)	2417
AjiI	CACGTC	1 cut(s)	1918
AjnI	CCWGG	7 cut(s)	458, 476, 947, 1028, 1826, 1893, 2425
AleI	CACNNNNGTG	3 cut(s)	1492, 2027, 2420
Alw21I	GWGCWC	2 cut(s)	1928, 1994
Alw26I	GTCTC	6 cut(s)	63, 461, 494, 1722, 2159, 2459
Alw44I	GTGCAC	1 cut(s)	1990
AlwI	GGATC	4 cut(s)	710, 724, 2005, 2231
AlwNI	CAGNNNCTG	1 cut(s)	759
Ama87I	CYCGRG	2 cut(s)	1238, 1640
Aor13HI	TCCGGA	2 cut(s)	862, 877
AoxI	GGCC	8 cut(s)	63, 118, 319, 632, 1404, 1608, 1956, 2327
ApaLI	GTGCAC	1 cut(s)	1990
ApeKI	GCWGC	9 cut(s)	200, 207, 227, 269, 648, 1684, 1859, 2159, 2202
ApoI	RAATTY	4 cut(s)	182, 290, 858, 1845
ArsI	GACNNNNNNTTYG	4 cut(s)	1107, 1139, 1581, 1613
AsiGI	ACCGGT	1 cut(s)	91
AspLEI	GCGC	5 cut(s)	626, 1145, 1875, 2138, 2435
AspS9I	GGNCC	6 cut(s)	632, 1033, 1280, 1608, 1782, 2193
AsuC2I	CCSGG	1 cut(s)	2057
AsuHPI	GGTGA	7 cut(s)	66, 97, 304, 436, 937, 2098, 2402
AsuII	TTCGAA	2 cut(s)	854, 1008
AvaI	CYCGRG	2 cut(s)	1238, 1640
AvaII	GGWCC	4 cut(s)	1033, 1280, 1782, 2193
BaeGI	GKGCMC	2 cut(s)	1901, 1994
BanI	GGYRCC	1 cut(s)	1142
BanII	GRGCYC	2 cut(s)	1331, 1660
BarI	GAAGNNNNNNTAC	2 cut(s)	1094, 1126
BauI	CACGAG	1 cut(s)	1863
BbrPI	CACGTG	3 cut(s)	61, 1083, 1582
Bbv12I	GWGCWC	2 cut(s)	1928, 1994
BbvI	GCAGC	9 cut(s)	212, 219, 239, 281, 660, 1671, 1846, 2171, 2189
BceAI	ACGGC	5 cut(s)	1618, 1646, 1853, 2314, 2342
BciT130I	CCWGG	7 cut(s)	460, 478, 949, 1030, 1828, 1895, 2427
BciVI	GTATCC	2 cut(s)	484, 672
BclI	TGATCA	1 cut(s)	2224
BcnI	CCSGG	1 cut(s)	2057
BcoDI	GTCTC	6 cut(s)	63, 461, 494, 1722, 2159, 2459
BcuI	ACTAGT	1 cut(s)	2417
BfaI	CTAG	2 cut(s)	2051, 2418
BfmI	CTRYAG	1 cut(s)	110
BfoI	RGCGCY	1 cut(s)	1146
BfuI	GTATCC	2 cut(s)	484, 672
BglI	GCCNNNNNGGC	1 cut(s)	126
BglII	AGATCT	1 cut(s)	1242
BlpI	GCTNAGC	1 cut(s)	1317
BmcAI	AGTACT	1 cut(s)	869
Bme1390I	CCNGG	8 cut(s)	460, 478, 949, 1030, 1828, 1895, 2057, 2427
Bme18I	GGWCC	4 cut(s)	1033, 1280, 1782, 2193
BmeT110I	CYCGRG	2 cut(s)	1238, 1640
BmgBI	CACGTC	1 cut(s)	1918
BmgT120I	GGNCC	6 cut(s)	632, 1033, 1280, 1608, 1782, 2193
BmiI	GGNNCC	8 cut(s)	1034, 1088, 1144, 1609, 1657, 1783, 1784, 1841
BmrFI	CCNGG	8 cut(s)	460, 478, 949, 1030, 1828, 1895, 2057, 2427
BmrI	ACTGGG	1 cut(s)	769
BmsI	GCATC	5 cut(s)	509, 541, 1210, 1879, 2313
BmuI	ACTGGG	1 cut(s)	769
BplI	GAGNNNNNCTC	2 cut(s)	1673, 1705
Bpu1102I	GCTNAGC	1 cut(s)	1317
Bpu14I	TTCGAA	2 cut(s)	854, 1008
BpuEI	CTTGAG	1 cut(s)	2378
BpuMI	CCSGG	1 cut(s)	2057
BsaAI	YACGTR	4 cut(s)	61, 297, 1083, 1582
BsaBI	GATNNNNATC	2 cut(s)	382, 2015
BsaHI	GRCGYC	2 cut(s)	1143, 2071
BsaWI	WCCGGW	6 cut(s)	91, 699, 743, 862, 877, 958
BsaXI	ACNNNNNCTCC	8 cut(s)	338, 368, 514, 544, 1078, 1108, 1824, 1854
Bsc4I	CCNNNNNNNGG	5 cut(s)	61, 273, 1035, 1467, 1711
Bse118I	RCCGGY	5 cut(s)	91, 404, 1035, 1139, 1376
Bse1I	ACTGG	2 cut(s)	764, 1106
Bse3DI	GCAATG	2 cut(s)	1022, 2366
Bse8I	GATNNNNATC	2 cut(s)	382, 2015
BseAI	TCCGGA	2 cut(s)	862, 877
BseBI	CCWGG	7 cut(s)	460, 478, 949, 1030, 1828, 1895, 2427
BseGI	GGATG	7 cut(s)	212, 330, 500, 769, 1078, 1905, 1948
BseJI	GATNNNNATC	2 cut(s)	382, 2015
BseLI	CCNNNNNNNGG	5 cut(s)	61, 273, 1035, 1467, 1711
BseMI	GCAATG	2 cut(s)	1022, 2366
BseMII	CTCAG	9 cut(s)	408, 734, 1267, 1331, 1476, 1711, 2178, 2454, 2490
BseNI	ACTGG	2 cut(s)	764, 1106
BseRI	GAGGAG	1 cut(s)	232
BseSI	GKGCMC	2 cut(s)	1901, 1994
BseX3I	CGGCCG	1 cut(s)	2327
BseXI	GCAGC	9 cut(s)	212, 219, 239, 281, 660, 1671, 1846, 2171, 2189
BsgI	GTGCAG	1 cut(s)	1845
Bsh1236I	CGCG	2 cut(s)	426, 1779
Bsh1285I	CGRYCG	6 cut(s)	96, 376, 669, 905, 2028, 2330
BshFI	GGCC	8 cut(s)	65, 120, 321, 634, 1406, 1610, 1958, 2329
BshNI	GGYRCC	1 cut(s)	1142
BshTI	ACCGGT	1 cut(s)	91
BsiEI	CGRYCG	6 cut(s)	96, 376, 669, 905, 2028, 2330
BsiHKAI	GWGCWC	2 cut(s)	1928, 1994
BsiHKCI	CYCGRG	2 cut(s)	1238, 1640
BslFI	GGGAC	5 cut(s)	752, 1046, 1277, 1795, 1845
BslI	CCNNNNNNNGG	5 cut(s)	61, 273, 1035, 1467, 1711
BsmAI	GTCTC	6 cut(s)	63, 461, 494, 1722, 2159, 2459
BsmBI	CGTCTC	2 cut(s)	2159, 2459
BsmFI	GGGAC	5 cut(s)	752, 1046, 1277, 1795, 1845
BsnI	GGCC	8 cut(s)	65, 120, 321, 634, 1406, 1610, 1958, 2329
BsoBI	CYCGRG	2 cut(s)	1238, 1640
Bsp119I	TTCGAA	2 cut(s)	854, 1008
Bsp1286I	GDGCHC	5 cut(s)	1331, 1660, 1901, 1928, 1994
Bsp13I	TCCGGA	2 cut(s)	862, 877
Bsp1407I	TGTACA	2 cut(s)	1110, 1584
Bsp1720I	GCTNAGC	1 cut(s)	1317
Bsp19I	CCATGG	1 cut(s)	684
BspANI	GGCC	8 cut(s)	65, 120, 321, 634, 1406, 1610, 1958, 2329
BspCNI	CTCAG	9 cut(s)	407, 733, 1266, 1330, 1475, 1710, 2179, 2455, 2489
BspEI	TCCGGA	2 cut(s)	862, 877
BspFNI	CGCG	2 cut(s)	426, 1779
BspLI	GGNNCC	8 cut(s)	1034, 1088, 1144, 1609, 1657, 1783, 1784, 1841
BspPI	GGATC	4 cut(s)	710, 724, 2005, 2231
BspQI	GCTCTTC	1 cut(s)	1916
BspT104I	TTCGAA	2 cut(s)	854, 1008
BspT107I	GGYRCC	1 cut(s)	1142
BsrBI	CCGCTC	1 cut(s)	899
BsrDI	GCAATG	2 cut(s)	1022, 2366
BsrFI	RCCGGY	5 cut(s)	91, 404, 1035, 1139, 1376
BsrGI	TGTACA	2 cut(s)	1110, 1584
BsrI	ACTGG	2 cut(s)	764, 1106
BssAI	RCCGGY	5 cut(s)	91, 404, 1035, 1139, 1376
BssNAI	GTATAC	1 cut(s)	1762
BssNI	GRCGYC	2 cut(s)	1143, 2071
BssSI	CACGAG	1 cut(s)	1863
BssT1I	CCWWGG	3 cut(s)	684, 1090, 1959
Bst1107I	GTATAC	1 cut(s)	1762
Bst2BI	CACGAG	1 cut(s)	1863
Bst2UI	CCWGG	7 cut(s)	460, 478, 949, 1030, 1828, 1895, 2427
Bst4CI	ACNGT	7 cut(s)	114, 472, 833, 1512, 1903, 2029, 2086
Bst6I	CTCTTC	3 cut(s)	250, 395, 1916
BstACI	GRCGYC	2 cut(s)	1143, 2071
BstAUI	TGTACA	2 cut(s)	1110, 1584
BstBAI	YACGTR	4 cut(s)	61, 297, 1083, 1582
BstBI	TTCGAA	2 cut(s)	854, 1008
BstC8I	GCNNGC	7 cut(s)	205, 406, 1137, 1141, 1365, 1777, 2157
BstDEI	CTNAG	9 cut(s)	394, 720, 1253, 1317, 1462, 1697, 2187, 2463, 2476
BstDSI	CCRYGG	1 cut(s)	684
BstF5I	GGATG	7 cut(s)	212, 330, 500, 769, 1078, 1905, 1948
BstFNI	CGCG	2 cut(s)	426, 1779
BstH2I	RGCGCY	1 cut(s)	1146
BstHHI	GCGC	5 cut(s)	626, 1145, 1875, 2138, 2435
BstMAI	GTCTC	6 cut(s)	63, 461, 494, 1722, 2159, 2459
BstMCI	CGRYCG	6 cut(s)	96, 376, 669, 905, 2028, 2330
BstMWI	GCNNNNNNNGC	5 cut(s)	126, 1184, 1303, 1373, 2144
BstNI	CCWGG	7 cut(s)	460, 478, 949, 1030, 1828, 1895, 2427
BstNSI	RCATGY	3 cut(s)	1367, 1598, 2159
BstSCI	CCNGG	8 cut(s)	458, 476, 947, 1028, 1826, 1893, 2055, 2425
BstSFI	CTRYAG	1 cut(s)	110
BstSLI	GKGCMC	2 cut(s)	1901, 1994
BstSNI	TACGTA	1 cut(s)	297
BstUI	CGCG	2 cut(s)	426, 1779
BstV1I	GCAGC	9 cut(s)	212, 219, 239, 281, 660, 1671, 1846, 2171, 2189
BstX2I	RGATCY	3 cut(s)	716, 1242, 2010
BstXI	CCANNNNNNTGG	6 cut(s)	203, 691, 1066, 1097, 1200, 2213
BstYI	RGATCY	3 cut(s)	716, 1242, 2010
BstZ17I	GTATAC	1 cut(s)	1762
BstZI	CGGCCG	1 cut(s)	2327
BsuI	GTATCC	2 cut(s)	484, 672
BsuRI	GGCC	8 cut(s)	65, 120, 321, 634, 1406, 1610, 1958, 2329
BtgI	CCRYGG	1 cut(s)	684
BtgZI	GCGATG	3 cut(s)	1039, 1357, 1902
BtrI	CACGTC	1 cut(s)	1918
BtsCI	GGATG	7 cut(s)	212, 330, 500, 769, 1078, 1905, 1948
BtsI	GCAGTG	1 cut(s)	1455
BtsIMutI	CAGTG	7 cut(s)	119, 1113, 1178, 1261, 1455, 1508, 1899
Cac8I	GCNNGC	7 cut(s)	205, 406, 1137, 1141, 1365, 1777, 2157
CaiI	CAGNNNCTG	1 cut(s)	759
CfoI	GCGC	5 cut(s)	626, 1145, 1875, 2138, 2435
Cfr10I	RCCGGY	5 cut(s)	91, 404, 1035, 1139, 1376
Cfr13I	GGNCC	6 cut(s)	632, 1033, 1280, 1608, 1782, 2193
CseI	GACGC	1 cut(s)	461
Csp6I	GTAC	7 cut(s)	298, 340, 868, 1111, 1585, 1734, 2101
CspAI	ACCGGT	1 cut(s)	91
CspCI	CAANNNNNGTGG	4 cut(s)	1600, 1635, 2296, 2331
CviAII	CATG	8 cut(s)	589, 685, 1364, 1595, 1771, 2002, 2156, 2245
CviQI	GTAC	7 cut(s)	298, 340, 868, 1111, 1585, 1734, 2101
DdeI	CTNAG	9 cut(s)	394, 720, 1253, 1317, 1462, 1697, 2187, 2463, 2476
DinI	GGCGCC	1 cut(s)	1144
DrdI	GACNNNNNNGTC	1 cut(s)	2002
DseDI	GACNNNNNNGTC	1 cut(s)	2002
EaeI	YGGCCR	4 cut(s)	63, 319, 1956, 2327
EagI	CGGCCG	1 cut(s)	2327
Eam1104I	CTCTTC	3 cut(s)	250, 395, 1916
EarI	CTCTTC	3 cut(s)	250, 395, 1916
EciI	GGCGGA	2 cut(s)	729, 1085
EclXI	CGGCCG	1 cut(s)	2327
Eco105I	TACGTA	1 cut(s)	297
Eco130I	CCWWGG	3 cut(s)	684, 1090, 1959
Eco24I	GRGCYC	2 cut(s)	1331, 1660
Eco32I	GATATC	1 cut(s)	172
Eco47I	GGWCC	4 cut(s)	1033, 1280, 1782, 2193
Eco52I	CGGCCG	1 cut(s)	2327
Eco57I	CTGAAG	1 cut(s)	1525
Eco72I	CACGTG	3 cut(s)	61, 1083, 1582
Eco88I	CYCGRG	2 cut(s)	1238, 1640
EcoO109I	RGGNCCY	1 cut(s)	1782
EcoRII	CCWGG	7 cut(s)	458, 476, 947, 1028, 1826, 1893, 2425
EcoRV	GATATC	1 cut(s)	172
EcoT14I	CCWWGG	3 cut(s)	684, 1090, 1959
EcoT38I	GRGCYC	2 cut(s)	1331, 1660
EgeI	GGCGCC	1 cut(s)	1144
EheI	GGCGCC	1 cut(s)	1144
ErhI	CCWWGG	3 cut(s)	684, 1090, 1959
Esp3I	CGTCTC	2 cut(s)	2159, 2459
FaeI	CATG	8 cut(s)	592, 688, 1367, 1598, 1774, 2005, 2159, 2248
FaqI	GGGAC	5 cut(s)	752, 1046, 1277, 1795, 1845
FatI	CATG	8 cut(s)	588, 684, 1363, 1594, 1770, 2001, 2155, 2244
FauI	CCCGC	3 cut(s)	1302, 1598, 1772
FbaI	TGATCA	1 cut(s)	2224
FblI	GTMKAC	4 cut(s)	84, 696, 1599, 1761
FokI	GGATG	7 cut(s)	199, 317, 487, 776, 1085, 1892, 1955
FriOI	GRGCYC	2 cut(s)	1331, 1660
FspBI	CTAG	2 cut(s)	2051, 2418
FspI	TGCGCA	1 cut(s)	2137
GlaI	GCGC	5 cut(s)	625, 1144, 1874, 2137, 2434
HaeII	RGCGCY	1 cut(s)	1146
HaeIII	GGCC	8 cut(s)	65, 120, 321, 634, 1406, 1610, 1958, 2329
HgaI	GACGC	1 cut(s)	461
HhaI	GCGC	5 cut(s)	626, 1145, 1875, 2138, 2435
Hin1I	GRCGYC	2 cut(s)	1143, 2071
Hin1II	CATG	8 cut(s)	592, 688, 1367, 1598, 1774, 2005, 2159, 2248
Hin6I	GCGC	5 cut(s)	624, 1143, 1873, 2136, 2433
HinP1I	GCGC	5 cut(s)	624, 1143, 1873, 2136, 2433
HincII	GTYRAC	4 cut(s)	139, 1206, 1600, 2075
HindII	GTYRAC	4 cut(s)	139, 1206, 1600, 2075
HinfI	GANTC	8 cut(s)	71, 573, 965, 1236, 1259, 1270, 1643, 2444
HphI	GGTGA	7 cut(s)	66, 97, 304, 436, 937, 2098, 2402
Hpy99I	CGWCG	5 cut(s)	455, 713, 1127, 1604, 2073
HpyAV	CCTTC	7 cut(s)	87, 428, 571, 1004, 1889, 2242, 2422
HpyCH4III	ACNGT	7 cut(s)	114, 472, 833, 1512, 1903, 2029, 2086
HpyF10VI	GCNNNNNNNGC	5 cut(s)	126, 1184, 1303, 1373, 2144
HpyF3I	CTNAG	9 cut(s)	394, 720, 1253, 1317, 1462, 1697, 2187, 2463, 2476
Hsp92I	GRCGYC	2 cut(s)	1143, 2071
Hsp92II	CATG	8 cut(s)	592, 688, 1367, 1598, 1774, 2005, 2159, 2248
HspAI	GCGC	5 cut(s)	624, 1143, 1873, 2136, 2433
KasI	GGCGCC	1 cut(s)	1142
KflI	GGGWCCC	1 cut(s)	1782
Kpn2I	TCCGGA	2 cut(s)	862, 877
KroI	GCCGGC	2 cut(s)	404, 1139
KroNI	GCCGGC	2 cut(s)	406, 1141
Ksp22I	TGATCA	1 cut(s)	2224
LguI	GCTCTTC	1 cut(s)	1916
LmnI	GCTCC	6 cut(s)	290, 880, 1086, 1655, 1845, 1856
Lsp1109I	GCAGC	9 cut(s)	212, 219, 239, 281, 660, 1671, 1846, 2171, 2189
LweI	GCATC	5 cut(s)	509, 541, 1210, 1879, 2313
MaeI	CTAG	2 cut(s)	2051, 2418
MaeIII	GTNAC	5 cut(s)	442, 1018, 1577, 1913, 2390
MbiI	CCGCTC	1 cut(s)	899
MboII	GAAGA	8 cut(s)	237, 382, 893, 1042, 1237, 1452, 1933, 2464
MflI	RGATCY	3 cut(s)	716, 1242, 2010
MhlI	GDGCHC	5 cut(s)	1331, 1660, 1901, 1928, 1994
MluCI	AATT	7 cut(s)	182, 290, 858, 1210, 1845, 2289, 2383
Mly113I	GGCGCC	1 cut(s)	1143
MlyI	GAGTC	3 cut(s)	65, 1230, 1652
MmeI	TCCRAC	2 cut(s)	444, 1494
MroI	TCCGGA	2 cut(s)	862, 877
MroNI	GCCGGC	2 cut(s)	404, 1139
MslI	CAYNNNNRTG	8 cut(s)	683, 689, 999, 1492, 1887, 2027, 2211, 2420
MspA1I	CMGCKG	4 cut(s)	203, 318, 2147, 2162
MspR9I	CCNGG	8 cut(s)	460, 478, 949, 1030, 1828, 1895, 2057, 2427
MvaI	CCWGG	7 cut(s)	460, 478, 949, 1030, 1828, 1895, 2427
MvnI	CGCG	2 cut(s)	426, 1779
MwoI	GCNNNNNNNGC	5 cut(s)	126, 1184, 1303, 1373, 2144
NaeI	GCCGGC	2 cut(s)	406, 1141
NarI	GGCGCC	1 cut(s)	1143
NciI	CCSGG	1 cut(s)	2057
NcoI	CCATGG	1 cut(s)	684
NgoMIV	GCCGGC	2 cut(s)	404, 1139
NlaIII	CATG	8 cut(s)	592, 688, 1367, 1598, 1774, 2005, 2159, 2248
NlaIV	GGNNCC	8 cut(s)	1034, 1088, 1144, 1609, 1657, 1783, 1784, 1841
NmeAIII	GCCGAG	2 cut(s)	253, 391
NmuCI	GTSAC	4 cut(s)	442, 1577, 1913, 2390
NsbI	TGCGCA	1 cut(s)	2137
NspI	RCATGY	3 cut(s)	1367, 1598, 2159
NspV	TTCGAA	2 cut(s)	854, 1008
OliI	CACNNNNGTG	3 cut(s)	1492, 2027, 2420
PaeI	GCATGC	2 cut(s)	1367, 2159
PaeR7I	CTCGAG	1 cut(s)	1238
PasI	CCCWGGG	2 cut(s)	1029, 1894
PciSI	GCTCTTC	1 cut(s)	1916
PcsI	WCGNNNNNNNCGW	2 cut(s)	530, 1128
PdiI	GCCGGC	2 cut(s)	406, 1141
PfeI	GAWTC	5 cut(s)	573, 965, 1259, 1270, 2444
PflFI	GACNNNGTC	2 cut(s)	134, 1121
PinAI	ACCGGT	1 cut(s)	91
Ple19I	CGATCG	1 cut(s)	669
PleI	GAGTC	3 cut(s)	65, 1230, 1651
PluTI	GGCGCC	1 cut(s)	1146
PmaCI	CACGTG	3 cut(s)	61, 1083, 1582
PmlI	CACGTG	3 cut(s)	61, 1083, 1582
PpsI	GAGTC	3 cut(s)	65, 1230, 1651
Ppu21I	YACGTR	4 cut(s)	61, 297, 1083, 1582
PpuMI	RGGWCCY	1 cut(s)	1782
Psp5II	RGGWCCY	1 cut(s)	1782
Psp6I	CCWGG	7 cut(s)	458, 476, 947, 1028, 1826, 1893, 2425
PspCI	CACGTG	3 cut(s)	61, 1083, 1582
PspGI	CCWGG	7 cut(s)	458, 476, 947, 1028, 1826, 1893, 2425
PspN4I	GGNNCC	8 cut(s)	1034, 1088, 1144, 1609, 1657, 1783, 1784, 1841
PspPI	GGNCC	6 cut(s)	632, 1033, 1280, 1608, 1782, 2193
PspPPI	RGGWCCY	1 cut(s)	1782
PstNI	CAGNNNCTG	1 cut(s)	759
PsuI	RGATCY	3 cut(s)	716, 1242, 2010
PsyI	GACNNNGTC	2 cut(s)	134, 1121
PvuI	CGATCG	1 cut(s)	669
PvuII	CAGCTG	2 cut(s)	203, 2147
RsaI	GTAC	7 cut(s)	299, 341, 869, 1112, 1586, 1735, 2102
RsaNI	GTAC	7 cut(s)	298, 340, 868, 1111, 1585, 1734, 2101
RseI	CAYNNNNRTG	8 cut(s)	683, 689, 999, 1492, 1887, 2027, 2211, 2420
SalI	GTCGAC	1 cut(s)	1598
SapI	GCTCTTC	1 cut(s)	1916
Sau96I	GGNCC	6 cut(s)	632, 1033, 1280, 1608, 1782, 2193
ScaI	AGTACT	1 cut(s)	869
SchI	GAGTC	3 cut(s)	65, 1230, 1652
ScrFI	CCNGG	8 cut(s)	460, 478, 949, 1030, 1828, 1895, 2057, 2427
SduI	GDGCHC	5 cut(s)	1331, 1660, 1901, 1928, 1994
SfaNI	GCATC	5 cut(s)	509, 541, 1210, 1879, 2313
SfcI	CTRYAG	1 cut(s)	110
SfoI	GGCGCC	1 cut(s)	1144
Sfr274I	CTCGAG	1 cut(s)	1238
SfuI	TTCGAA	2 cut(s)	854, 1008
SgrAI	CRCCGGYG	1 cut(s)	1376
SinI	GGWCC	4 cut(s)	1033, 1280, 1782, 2193
SlaI	CTCGAG	1 cut(s)	1238
SmiMI	CAYNNNNRTG	8 cut(s)	683, 689, 999, 1492, 1887, 2027, 2211, 2420
SmlI	CTYRAG	2 cut(s)	1238, 2357
SmoI	CTYRAG	2 cut(s)	1238, 2357
SnaBI	TACGTA	1 cut(s)	297
SpeI	ACTAGT	1 cut(s)	2417
SphI	GCATGC	2 cut(s)	1367, 2159
Sse9I	AATT	7 cut(s)	182, 290, 858, 1210, 1845, 2289, 2383
SspDI	GGCGCC	1 cut(s)	1142
SspMI	CTAG	2 cut(s)	2051, 2418
StyD4I	CCNGG	8 cut(s)	458, 476, 947, 1028, 1826, 1893, 2055, 2425
StyI	CCWWGG	3 cut(s)	684, 1090, 1959
TaaI	ACNGT	7 cut(s)	114, 472, 833, 1512, 1903, 2029, 2086
TaqII	GACCGA	3 cut(s)	390, 891, 1565
TasI	AATT	7 cut(s)	182, 290, 858, 1210, 1845, 2289, 2383
TatI	WGTACW	6 cut(s)	339, 867, 1110, 1584, 1733, 2100
TauI	GCSGC	7 cut(s)	120, 321, 365, 411, 826, 1529, 1958
TfiI	GAWTC	5 cut(s)	573, 965, 1259, 1270, 2444
TscAI	CASTG	7 cut(s)	119, 1113, 1185, 1261, 1462, 1515, 1906
TseFI	GTSAC	4 cut(s)	442, 1577, 1913, 2390
TseI	GCWGC	9 cut(s)	200, 207, 227, 269, 648, 1684, 1859, 2159, 2202
Tsp45I	GTSAC	4 cut(s)	442, 1577, 1913, 2390
TspGWI	ACGGA	6 cut(s)	165, 430, 631, 1248, 1688, 2437
TspRI	CASTG	7 cut(s)	119, 1113, 1185, 1261, 1462, 1515, 1906
Tth111I	GACNNNGTC	2 cut(s)	134, 1121
VneI	GTGCAC	1 cut(s)	1990
VpaK11BI	GGWCC	4 cut(s)	1033, 1280, 1782, 2193
XapI	RAATTY	4 cut(s)	182, 290, 858, 1845
XbaI	TCTAGA	1 cut(s)	2050
XceI	RCATGY	3 cut(s)	1367, 1598, 2159
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1824, 1850
XhoI	CTCGAG	1 cut(s)	1238
XmiI	GTMKAC	4 cut(s)	84, 696, 1599, 1761
XspI	CTAG	2 cut(s)	2051, 2418
ZraI	GACGTC	1 cut(s)	2072
ZrmI	AGTACT	1 cut(s)	869

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.