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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 196 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 383 |
| AciI | CCGC | 1 cut(s) | 215 |
| AcsI | RAATTY | 1 cut(s) | 165 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 383 |
| AgsI | TTSAA | 1 cut(s) | 257 |
| AjiI | CACGTC | 1 cut(s) | 38 |
| AluBI | AGCT | 1 cut(s) | 275 |
| AluI | AGCT | 1 cut(s) | 275 |
| Alw26I | GTCTC | 1 cut(s) | 61 |
| AoxI | GGCC | 1 cut(s) | 105 |
| ApeKI | GCWGC | 1 cut(s) | 275 |
| ApoI | RAATTY | 1 cut(s) | 165 |
| AspLEI | GCGC | 1 cut(s) | 209 |
| AspS9I | GGNCC | 1 cut(s) | 106 |
| AsuHPI | GGTGA | 3 cut(s) | 240, 282, 393 |
| AxyI | CCTNAGG | 1 cut(s) | 111 |
| BbvI | GCAGC | 1 cut(s) | 262 |
| BcoDI | GTCTC | 1 cut(s) | 61 |
| BfaI | CTAG | 1 cut(s) | 81 |
| BfuAI | ACCTGC | 1 cut(s) | 196 |
| BisI | GCNGC | 1 cut(s) | 276 |
| BlsI | GCNGC | 1 cut(s) | 277 |
| BmgBI | CACGTC | 1 cut(s) | 38 |
| BmgT120I | GGNCC | 1 cut(s) | 106 |
| BmiI | GGNNCC | 2 cut(s) | 108, 219 |
| BmsI | GCATC | 2 cut(s) | 218, 319 |
| BoxI | GACNNNNGTC | 1 cut(s) | 244 |
| BsaJI | CCNNGG | 1 cut(s) | 235 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 52, 82, 388, 418 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 383 |
| Bse21I | CCTNAGG | 1 cut(s) | 111 |
| BseDI | CCNNGG | 1 cut(s) | 235 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 383 |
| BseMII | CTCAG | 1 cut(s) | 125 |
| BseRI | GAGGAG | 2 cut(s) | 54, 57 |
| BseXI | GCAGC | 1 cut(s) | 262 |
| BsgI | GTGCAG | 1 cut(s) | 261 |
| Bsh1285I | CGRYCG | 1 cut(s) | 235 |
| BshFI | GGCC | 1 cut(s) | 107 |
| BsiEI | CGRYCG | 1 cut(s) | 235 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 383 |
| BsmAI | GTCTC | 1 cut(s) | 61 |
| BsmBI | CGTCTC | 1 cut(s) | 61 |
| BsmI | GAATGC | 1 cut(s) | 263 |
| BsnI | GGCC | 1 cut(s) | 107 |
| BspACI | CCGC | 1 cut(s) | 215 |
| BspANI | GGCC | 1 cut(s) | 107 |
| BspCNI | CTCAG | 2 cut(s) | 4, 124 |
| BspLI | GGNNCC | 2 cut(s) | 108, 219 |
| BspMI | ACCTGC | 1 cut(s) | 196 |
| BssECI | CCNNGG | 1 cut(s) | 235 |
| BstC8I | GCNNGC | 3 cut(s) | 134, 205, 334 |
| BstDEI | CTNAG | 2 cut(s) | 12, 111 |
| BstHHI | GCGC | 1 cut(s) | 209 |
| BstMAI | GTCTC | 1 cut(s) | 61 |
| BstMCI | CGRYCG | 1 cut(s) | 235 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 272 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 244 |
| BstV1I | GCAGC | 1 cut(s) | 262 |
| Bsu36I | CCTNAGG | 1 cut(s) | 111 |
| BsuRI | GGCC | 1 cut(s) | 107 |
| BtrI | CACGTC | 1 cut(s) | 38 |
| BveI | ACCTGC | 1 cut(s) | 196 |
| Cac8I | GCNNGC | 3 cut(s) | 134, 205, 334 |
| CfoI | GCGC | 1 cut(s) | 209 |
| Cfr13I | GGNCC | 1 cut(s) | 106 |
| CviJI | RGCY | 4 cut(s) | 107, 121, 275, 442 |
| CviKI_1 | RGCY | 4 cut(s) | 107, 121, 275, 442 |
| DdeI | CTNAG | 2 cut(s) | 12, 111 |
| Eco81I | CCTNAGG | 1 cut(s) | 111 |
| EcoO109I | RGGNCCY | 1 cut(s) | 106 |
| EcoRI | GAATTC | 1 cut(s) | 165 |
| EcoT22I | ATGCAT | 1 cut(s) | 364 |
| Esp3I | CGTCTC | 1 cut(s) | 61 |
| FaiI | YATR | 2 cut(s) | 21, 431 |
| Fnu4HI | GCNGC | 1 cut(s) | 276 |
| Fsp4HI | GCNGC | 1 cut(s) | 276 |
| FspBI | CTAG | 1 cut(s) | 81 |
| GlaI | GCGC | 1 cut(s) | 208 |
| GluI | GCNGC | 1 cut(s) | 276 |
| HaeIII | GGCC | 1 cut(s) | 107 |
| HhaI | GCGC | 1 cut(s) | 209 |
| Hin6I | GCGC | 1 cut(s) | 207 |
| HinP1I | GCGC | 1 cut(s) | 207 |
| HinfI | GANTC | 2 cut(s) | 23, 194 |
| HphI | GGTGA | 3 cut(s) | 240, 282, 393 |
| Hpy188III | TCNNGA | 2 cut(s) | 113, 302 |
| HpyCH4IV | ACGT | 3 cut(s) | 37, 54, 389 |
| HpyCH4V | TGCA | 3 cut(s) | 278, 336, 362 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 272 |
| HpyF3I | CTNAG | 2 cut(s) | 12, 111 |
| HpySE526I | ACGT | 3 cut(s) | 37, 54, 389 |
| HspAI | GCGC | 1 cut(s) | 207 |
| LpnPI | CCDG | 4 cut(s) | 98, 155, 201, 315 |
| Lsp1109I | GCAGC | 1 cut(s) | 262 |
| LweI | GCATC | 2 cut(s) | 218, 319 |
| MaeI | CTAG | 1 cut(s) | 81 |
| MaeII | ACGT | 3 cut(s) | 37, 54, 389 |
| MaeIII | GTNAC | 2 cut(s) | 246, 390 |
| MboII | GAAGA | 2 cut(s) | 338, 437 |
| MluCI | AATT | 2 cut(s) | 165, 311 |
| MlyI | GAGTC | 2 cut(s) | 32, 188 |
| MnlI | CCTC | 9 cut(s) | 75, 78, 99, 120, 230, 231, 307, 390, 414 |
| Mph1103I | ATGCAT | 1 cut(s) | 364 |
| MseI | TTAA | 2 cut(s) | 309, 357 |
| MslI | CAYNNNNRTG | 1 cut(s) | 256 |
| Mva1269I | GAATGC | 1 cut(s) | 263 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 272 |
| NlaIV | GGNNCC | 2 cut(s) | 108, 219 |
| NmuCI | GTSAC | 1 cut(s) | 246 |
| NsiI | ATGCAT | 1 cut(s) | 364 |
| PctI | GAATGC | 1 cut(s) | 263 |
| PflFI | GACNNNGTC | 1 cut(s) | 53 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 383 |
| PkrI | GCNGC | 1 cut(s) | 277 |
| PleI | GAGTC | 2 cut(s) | 31, 188 |
| PpsI | GAGTC | 2 cut(s) | 31, 188 |
| PshAI | GACNNNNGTC | 1 cut(s) | 244 |
| PspN4I | GGNNCC | 2 cut(s) | 108, 219 |
| PspPI | GGNCC | 1 cut(s) | 106 |
| PsyI | GACNNNGTC | 1 cut(s) | 53 |
| RseI | CAYNNNNRTG | 1 cut(s) | 256 |
| SaqAI | TTAA | 2 cut(s) | 309, 357 |
| SatI | GCNGC | 1 cut(s) | 276 |
| Sau96I | GGNCC | 1 cut(s) | 106 |
| SchI | GAGTC | 2 cut(s) | 32, 188 |
| SfaNI | GCATC | 2 cut(s) | 218, 319 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 256 |
| Sse9I | AATT | 2 cut(s) | 165, 311 |
| SsiI | CCGC | 1 cut(s) | 215 |
| SspI | AATATT | 1 cut(s) | 346 |
| SspMI | CTAG | 1 cut(s) | 81 |
| TaiI | ACGT | 3 cut(s) | 40, 57, 392 |
| TaqII | GACCGA | 1 cut(s) | 249 |
| TasI | AATT | 2 cut(s) | 165, 311 |
| Tru1I | TTAA | 2 cut(s) | 309, 357 |
| Tru9I | TTAA | 2 cut(s) | 309, 357 |
| TseFI | GTSAC | 1 cut(s) | 246 |
| TseI | GCWGC | 1 cut(s) | 275 |
| Tsp45I | GTSAC | 1 cut(s) | 246 |
| TspDTI | ATGAA | 1 cut(s) | 275 |
| TspGWI | ACGGA | 1 cut(s) | 338 |
| Tth111I | GACNNNGTC | 1 cut(s) | 53 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 383 |
| XapI | RAATTY | 1 cut(s) | 165 |
| XspI | CTAG | 1 cut(s) | 81 |
| Zsp2I | ATGCAT | 1 cut(s) | 364 |