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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 21 |
| AclWI | GGATC | 2 cut(s) | 257, 268 |
| AcsI | RAATTY | 2 cut(s) | 68, 242 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 21, 155 |
| AgsI | TTSAA | 6 cut(s) | 49, 74, 86, 165, 270, 357 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 142, 174 |
| AluBI | AGCT | 1 cut(s) | 82 |
| AluI | AGCT | 1 cut(s) | 82 |
| Alw26I | GTCTC | 1 cut(s) | 97 |
| AlwI | GGATC | 2 cut(s) | 257, 268 |
| AoxI | GGCC | 1 cut(s) | 198 |
| ApoI | RAATTY | 2 cut(s) | 68, 242 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 44, 242 |
| AspA2I | CCTAGG | 1 cut(s) | 277 |
| AspS9I | GGNCC | 1 cut(s) | 303 |
| AsuHPI | GGTGA | 2 cut(s) | 138, 307 |
| AvaII | GGWCC | 1 cut(s) | 303 |
| AvrII | CCTAGG | 1 cut(s) | 277 |
| BbsI | GAAGAC | 1 cut(s) | 47 |
| BceAI | ACGGC | 1 cut(s) | 368 |
| BcoDI | GTCTC | 1 cut(s) | 97 |
| BfaI | CTAG | 1 cut(s) | 278 |
| BlnI | CCTAGG | 1 cut(s) | 277 |
| Bme18I | GGWCC | 1 cut(s) | 303 |
| BmgT120I | GGNCC | 1 cut(s) | 303 |
| BmiI | GGNNCC | 1 cut(s) | 305 |
| BmsI | GCATC | 1 cut(s) | 393 |
| BpiI | GAAGAC | 1 cut(s) | 47 |
| BsaJI | CCNNGG | 2 cut(s) | 277, 384 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 21, 155 |
| BseDI | CCNNGG | 2 cut(s) | 277, 384 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 21, 155 |
| BshFI | GGCC | 1 cut(s) | 200 |
| BsiSI | CCGG | 1 cut(s) | 344 |
| BslFI | GGGAC | 1 cut(s) | 289 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 21, 155 |
| BsmAI | GTCTC | 1 cut(s) | 97 |
| BsmBI | CGTCTC | 1 cut(s) | 97 |
| BsmFI | GGGAC | 1 cut(s) | 289 |
| BsmI | GAATGC | 2 cut(s) | 288, 397 |
| BsnI | GGCC | 1 cut(s) | 200 |
| Bsp143I | GATC | 4 cut(s) | 33, 249, 273, 407 |
| BspANI | GGCC | 1 cut(s) | 200 |
| BspLI | GGNNCC | 1 cut(s) | 305 |
| BspPI | GGATC | 2 cut(s) | 257, 268 |
| BspQI | GCTCTTC | 1 cut(s) | 72 |
| BssECI | CCNNGG | 2 cut(s) | 277, 384 |
| BssMI | GATC | 4 cut(s) | 33, 249, 273, 407 |
| BssT1I | CCWWGG | 1 cut(s) | 277 |
| Bst6I | CTCTTC | 2 cut(s) | 72, 227 |
| BstC8I | GCNNGC | 1 cut(s) | 397 |
| BstDSI | CCRYGG | 1 cut(s) | 384 |
| BstKTI | GATC | 4 cut(s) | 36, 252, 276, 410 |
| BstMAI | GTCTC | 1 cut(s) | 97 |
| BstMBI | GATC | 4 cut(s) | 33, 249, 273, 407 |
| BstV2I | GAAGAC | 1 cut(s) | 47 |
| BstX2I | RGATCY | 2 cut(s) | 249, 273 |
| BstYI | RGATCY | 2 cut(s) | 249, 273 |
| BsuRI | GGCC | 1 cut(s) | 200 |
| BtgI | CCRYGG | 1 cut(s) | 384 |
| Cac8I | GCNNGC | 1 cut(s) | 397 |
| Cfr13I | GGNCC | 1 cut(s) | 303 |
| CviAII | CATG | 1 cut(s) | 209 |
| CviJI | RGCY | 3 cut(s) | 82, 200, 383 |
| CviKI_1 | RGCY | 3 cut(s) | 82, 200, 383 |
| DpnI | GATC | 4 cut(s) | 35, 251, 275, 409 |
| DpnII | GATC | 4 cut(s) | 33, 249, 273, 407 |
| Eam1104I | CTCTTC | 2 cut(s) | 72, 227 |
| EarI | CTCTTC | 2 cut(s) | 72, 227 |
| Eco130I | CCWWGG | 1 cut(s) | 277 |
| Eco147I | AGGCCT | 1 cut(s) | 200 |
| Eco47I | GGWCC | 1 cut(s) | 303 |
| EcoT14I | CCWWGG | 1 cut(s) | 277 |
| ErhI | CCWWGG | 1 cut(s) | 277 |
| Esp3I | CGTCTC | 1 cut(s) | 97 |
| FaeI | CATG | 1 cut(s) | 212 |
| FaiI | YATR | 2 cut(s) | 210, 326 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 66, 98 |
| FaqI | GGGAC | 1 cut(s) | 289 |
| FatI | CATG | 1 cut(s) | 208 |
| FspBI | CTAG | 1 cut(s) | 278 |
| HaeIII | GGCC | 1 cut(s) | 200 |
| HapII | CCGG | 1 cut(s) | 344 |
| Hin1II | CATG | 1 cut(s) | 212 |
| HinfI | GANTC | 1 cut(s) | 25 |
| HpaII | CCGG | 1 cut(s) | 344 |
| HphI | GGTGA | 2 cut(s) | 138, 307 |
| Hpy188I | TCNGA | 2 cut(s) | 30, 335 |
| Hpy188III | TCNNGA | 3 cut(s) | 37, 247, 253 |
| HpyAV | CCTTC | 4 cut(s) | 148, 190, 427, 441 |
| HpyCH4IV | ACGT | 1 cut(s) | 44 |
| HpyCH4V | TGCA | 1 cut(s) | 324 |
| HpySE526I | ACGT | 1 cut(s) | 44 |
| Hsp92II | CATG | 1 cut(s) | 212 |
| Kzo9I | GATC | 4 cut(s) | 33, 249, 273, 407 |
| LguI | GCTCTTC | 1 cut(s) | 72 |
| LpnPI | CCDG | 1 cut(s) | 357 |
| LweI | GCATC | 1 cut(s) | 393 |
| MaeI | CTAG | 1 cut(s) | 278 |
| MaeII | ACGT | 1 cut(s) | 44 |
| MalI | GATC | 4 cut(s) | 35, 251, 275, 409 |
| MboI | GATC | 4 cut(s) | 33, 249, 273, 407 |
| MflI | RGATCY | 2 cut(s) | 249, 273 |
| MluCI | AATT | 3 cut(s) | 68, 242, 369 |
| MmeI | TCCRAC | 2 cut(s) | 358, 445 |
| MnlI | CCTC | 5 cut(s) | 12, 101, 230, 311, 423 |
| MroXI | GAANNNNTTC | 2 cut(s) | 44, 242 |
| MseI | TTAA | 2 cut(s) | 372, 469 |
| MspI | CCGG | 1 cut(s) | 344 |
| Mva1269I | GAATGC | 2 cut(s) | 288, 397 |
| NdeII | GATC | 4 cut(s) | 33, 249, 273, 407 |
| NlaIII | CATG | 1 cut(s) | 212 |
| NlaIV | GGNNCC | 1 cut(s) | 305 |
| PceI | AGGCCT | 1 cut(s) | 200 |
| PciSI | GCTCTTC | 1 cut(s) | 72 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 403 |
| PctI | GAATGC | 2 cut(s) | 288, 397 |
| PdmI | GAANNNNTTC | 2 cut(s) | 44, 242 |
| PfeI | GAWTC | 1 cut(s) | 25 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 21 |
| PspN4I | GGNNCC | 1 cut(s) | 305 |
| PspPI | GGNCC | 1 cut(s) | 303 |
| PsuI | RGATCY | 2 cut(s) | 249, 273 |
| SapI | GCTCTTC | 1 cut(s) | 72 |
| SaqAI | TTAA | 2 cut(s) | 372, 469 |
| Sau3AI | GATC | 4 cut(s) | 33, 249, 273, 407 |
| Sau96I | GGNCC | 1 cut(s) | 303 |
| SetI | ASST | 5 cut(s) | 23, 47, 84, 222, 297 |
| SfaNI | GCATC | 1 cut(s) | 393 |
| SinI | GGWCC | 1 cut(s) | 303 |
| Sse9I | AATT | 3 cut(s) | 68, 242, 369 |
| SseBI | AGGCCT | 1 cut(s) | 200 |
| SspMI | CTAG | 1 cut(s) | 278 |
| StuI | AGGCCT | 1 cut(s) | 200 |
| StyI | CCWWGG | 1 cut(s) | 277 |
| TaiI | ACGT | 1 cut(s) | 47 |
| TaqI | TCGA | 2 cut(s) | 254, 316 |
| TasI | AATT | 3 cut(s) | 68, 242, 369 |
| TfiI | GAWTC | 1 cut(s) | 25 |
| Tru1I | TTAA | 2 cut(s) | 372, 469 |
| Tru9I | TTAA | 2 cut(s) | 372, 469 |
| TspDTI | ATGAA | 1 cut(s) | 225 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 21 |
| VpaK11BI | GGWCC | 1 cut(s) | 303 |
| XapI | RAATTY | 2 cut(s) | 68, 242 |
| XmaJI | CCTAGG | 1 cut(s) | 277 |
| XmnI | GAANNNNTTC | 2 cut(s) | 44, 242 |
| XspI | CTAG | 1 cut(s) | 278 |