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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 129 |
| AciI | CCGC | 3 cut(s) | 237, 773, 788 |
| AcsI | RAATTY | 2 cut(s) | 664, 848 |
| AflIII | ACRYGT | 1 cut(s) | 731 |
| AgsI | TTSAA | 3 cut(s) | 224, 302, 559 |
| AjiI | CACGTC | 1 cut(s) | 734 |
| AjnI | CCWGG | 1 cut(s) | 736 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 332, 364, 573, 605 |
| AluBI | AGCT | 4 cut(s) | 319, 404, 526, 547 |
| AluI | AGCT | 4 cut(s) | 319, 404, 526, 547 |
| Alw26I | GTCTC | 1 cut(s) | 470 |
| Ama87I | CYCGRG | 1 cut(s) | 527 |
| AoxI | GGCC | 1 cut(s) | 801 |
| ApoI | RAATTY | 2 cut(s) | 664, 848 |
| AseI | ATTAAT | 1 cut(s) | 414 |
| AspS9I | GGNCC | 1 cut(s) | 802 |
| AsuHPI | GGTGA | 2 cut(s) | 714, 752 |
| AvaI | CYCGRG | 1 cut(s) | 527 |
| BauI | CACGAG | 1 cut(s) | 669 |
| BccI | CCATC | 2 cut(s) | 466, 691 |
| BciT130I | CCWGG | 1 cut(s) | 738 |
| BcoDI | GTCTC | 1 cut(s) | 470 |
| BfaI | CTAG | 1 cut(s) | 777 |
| Bme1390I | CCNGG | 1 cut(s) | 738 |
| BmeT110I | CYCGRG | 1 cut(s) | 527 |
| BmgBI | CACGTC | 1 cut(s) | 734 |
| BmgT120I | GGNCC | 1 cut(s) | 802 |
| BmiI | GGNNCC | 1 cut(s) | 804 |
| BmrFI | CCNGG | 1 cut(s) | 738 |
| BmsI | GCATC | 2 cut(s) | 133, 145 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 641, 673 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 748, 778 |
| BseBI | CCWGG | 1 cut(s) | 738 |
| BseGI | GGATG | 3 cut(s) | 358, 436, 622 |
| BseMII | CTCAG | 1 cut(s) | 176 |
| BseRI | GAGGAG | 1 cut(s) | 587 |
| BshFI | GGCC | 1 cut(s) | 803 |
| BsiHKCI | CYCGRG | 1 cut(s) | 527 |
| BslFI | GGGAC | 1 cut(s) | 268 |
| BsmAI | GTCTC | 1 cut(s) | 470 |
| BsmFI | GGGAC | 1 cut(s) | 268 |
| BsnI | GGCC | 1 cut(s) | 803 |
| BsoBI | CYCGRG | 1 cut(s) | 527 |
| BspACI | CCGC | 3 cut(s) | 237, 773, 788 |
| BspANI | GGCC | 1 cut(s) | 803 |
| BspCNI | CTCAG | 1 cut(s) | 175 |
| BspLI | GGNNCC | 1 cut(s) | 804 |
| BspQI | GCTCTTC | 2 cut(s) | 309, 537 |
| BssSI | CACGAG | 1 cut(s) | 669 |
| Bst2BI | CACGAG | 1 cut(s) | 669 |
| Bst2UI | CCWGG | 1 cut(s) | 738 |
| Bst4CI | ACNGT | 4 cut(s) | 9, 28, 220, 464 |
| Bst6I | CTCTTC | 6 cut(s) | 309, 414, 495, 537, 600, 765 |
| BstC8I | GCNNGC | 1 cut(s) | 830 |
| BstDEI | CTNAG | 4 cut(s) | 84, 162, 320, 548 |
| BstF5I | GGATG | 3 cut(s) | 358, 436, 622 |
| BstMAI | GTCTC | 1 cut(s) | 470 |
| BstNI | CCWGG | 1 cut(s) | 738 |
| BstNSI | RCATGY | 1 cut(s) | 35 |
| BstSCI | CCNGG | 1 cut(s) | 736 |
| BsuRI | GGCC | 1 cut(s) | 803 |
| BtrI | CACGTC | 1 cut(s) | 734 |
| BtsCI | GGATG | 3 cut(s) | 358, 436, 622 |
| BtsI | GCAGTG | 1 cut(s) | 77 |
| BtsIMutI | CAGTG | 5 cut(s) | 33, 48, 77, 89, 469 |
| Cac8I | GCNNGC | 1 cut(s) | 830 |
| Cfr13I | GGNCC | 1 cut(s) | 802 |
| CviAII | CATG | 4 cut(s) | 32, 44, 244, 261 |
| CviJI | RGCY | 8 cut(s) | 319, 348, 404, 526, 547, 656, 803, 817 |
| CviKI_1 | RGCY | 8 cut(s) | 319, 348, 404, 526, 547, 656, 803, 817 |
| DdeI | CTNAG | 4 cut(s) | 84, 162, 320, 548 |
| Eam1104I | CTCTTC | 6 cut(s) | 309, 414, 495, 537, 600, 765 |
| EarI | CTCTTC | 6 cut(s) | 309, 414, 495, 537, 600, 765 |
| EciI | GGCGGA | 1 cut(s) | 777 |
| Eco88I | CYCGRG | 1 cut(s) | 527 |
| EcoO109I | RGGNCCY | 1 cut(s) | 802 |
| EcoRI | GAATTC | 1 cut(s) | 664 |
| EcoRII | CCWGG | 1 cut(s) | 736 |
| FaeI | CATG | 4 cut(s) | 35, 47, 247, 264 |
| FaiI | YATR | 9 cut(s) | 13, 15, 33, 45, 129, 198, 245, 262, 692 |
| FaqI | GGGAC | 1 cut(s) | 268 |
| FatI | CATG | 4 cut(s) | 31, 43, 243, 260 |
| FauI | CCCGC | 1 cut(s) | 230 |
| FokI | GGATG | 3 cut(s) | 365, 443, 629 |
| FspBI | CTAG | 1 cut(s) | 777 |
| HaeIII | GGCC | 1 cut(s) | 803 |
| Hin1II | CATG | 4 cut(s) | 35, 47, 247, 264 |
| HinfI | GANTC | 2 cut(s) | 719, 752 |
| HphI | GGTGA | 2 cut(s) | 714, 752 |
| Hpy188I | TCNGA | 1 cut(s) | 117 |
| Hpy188III | TCNNGA | 4 cut(s) | 188, 529, 669, 809 |
| HpyAV | CCTTC | 3 cut(s) | 244, 502, 604 |
| HpyCH4III | ACNGT | 4 cut(s) | 9, 28, 220, 464 |
| HpyCH4IV | ACGT | 1 cut(s) | 733 |
| HpyCH4V | TGCA | 4 cut(s) | 31, 62, 146, 260 |
| HpyF3I | CTNAG | 4 cut(s) | 84, 162, 320, 548 |
| HpySE526I | ACGT | 1 cut(s) | 733 |
| Hsp92II | CATG | 4 cut(s) | 35, 47, 247, 264 |
| LguI | GCTCTTC | 2 cut(s) | 309, 537 |
| LpnPI | CCDG | 4 cut(s) | 247, 674, 723, 750 |
| LweI | GCATC | 2 cut(s) | 133, 145 |
| MaeI | CTAG | 1 cut(s) | 777 |
| MaeII | ACGT | 1 cut(s) | 733 |
| MaeIII | GTNAC | 6 cut(s) | 3, 34, 46, 75, 87, 720 |
| MfeI | CAATTG | 1 cut(s) | 680 |
| MlyI | GAGTC | 2 cut(s) | 728, 746 |
| MnlI | CCTC | 7 cut(s) | 415, 565, 619, 667, 774, 795, 816 |
| MseI | TTAA | 2 cut(s) | 414, 853 |
| MslI | CAYNNNNRTG | 3 cut(s) | 201, 414, 695 |
| MspA1I | CMGCKG | 1 cut(s) | 237 |
| MspR9I | CCNGG | 1 cut(s) | 738 |
| MunI | CAATTG | 1 cut(s) | 680 |
| MvaI | CCWGG | 1 cut(s) | 738 |
| NlaIII | CATG | 4 cut(s) | 35, 47, 247, 264 |
| NlaIV | GGNNCC | 1 cut(s) | 804 |
| NmuCI | GTSAC | 4 cut(s) | 46, 75, 87, 720 |
| NspI | RCATGY | 1 cut(s) | 35 |
| PaeR7I | CTCGAG | 1 cut(s) | 527 |
| PciSI | GCTCTTC | 2 cut(s) | 309, 537 |
| PleI | GAGTC | 2 cut(s) | 727, 746 |
| PpsI | GAGTC | 2 cut(s) | 727, 746 |
| PshBI | ATTAAT | 1 cut(s) | 414 |
| PsiI | TTATAA | 1 cut(s) | 129 |
| Psp6I | CCWGG | 1 cut(s) | 736 |
| PspGI | CCWGG | 1 cut(s) | 736 |
| PspN4I | GGNNCC | 1 cut(s) | 804 |
| PspPI | GGNCC | 1 cut(s) | 802 |
| RseI | CAYNNNNRTG | 3 cut(s) | 201, 414, 695 |
| SapI | GCTCTTC | 2 cut(s) | 309, 537 |
| SaqAI | TTAA | 2 cut(s) | 414, 853 |
| Sau96I | GGNCC | 1 cut(s) | 802 |
| SchI | GAGTC | 2 cut(s) | 728, 746 |
| ScrFI | CCNGG | 1 cut(s) | 738 |
| SetI | ASST | 7 cut(s) | 321, 406, 528, 549, 639, 736, 742 |
| SfaNI | GCATC | 2 cut(s) | 133, 145 |
| Sfr274I | CTCGAG | 1 cut(s) | 527 |
| SlaI | CTCGAG | 1 cut(s) | 527 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 201, 414, 695 |
| SmlI | CTYRAG | 1 cut(s) | 527 |
| SmoI | CTYRAG | 1 cut(s) | 527 |
| SsiI | CCGC | 3 cut(s) | 237, 773, 788 |
| SspMI | CTAG | 1 cut(s) | 777 |
| StyD4I | CCNGG | 1 cut(s) | 736 |
| TaaI | ACNGT | 4 cut(s) | 9, 28, 220, 464 |
| TaiI | ACGT | 1 cut(s) | 736 |
| TaqI | TCGA | 1 cut(s) | 528 |
| Tru1I | TTAA | 2 cut(s) | 414, 853 |
| Tru9I | TTAA | 2 cut(s) | 414, 853 |
| TscAI | CASTG | 5 cut(s) | 33, 55, 84, 96, 469 |
| TseFI | GTSAC | 4 cut(s) | 46, 75, 87, 720 |
| Tsp45I | GTSAC | 4 cut(s) | 46, 75, 87, 720 |
| TspDTI | ATGAA | 5 cut(s) | 369, 432, 453, 465, 513 |
| TspRI | CASTG | 5 cut(s) | 33, 55, 84, 96, 469 |
| VspI | ATTAAT | 1 cut(s) | 414 |
| XapI | RAATTY | 2 cut(s) | 664, 848 |
| XceI | RCATGY | 1 cut(s) | 35 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 241 |
| XhoI | CTCGAG | 1 cut(s) | 527 |
| XspI | CTAG | 1 cut(s) | 777 |