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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 298 |
| AcsI | RAATTY | 1 cut(s) | 237 |
| AgsI | TTSAA | 1 cut(s) | 481 |
| AjiI | CACGTC | 1 cut(s) | 110 |
| AjnI | CCWGG | 1 cut(s) | 112 |
| Alw26I | GTCTC | 1 cut(s) | 102 |
| AoxI | GGCC | 1 cut(s) | 177 |
| ApoI | RAATTY | 1 cut(s) | 237 |
| AseI | ATTAAT | 1 cut(s) | 441 |
| AspLEI | GCGC | 1 cut(s) | 282 |
| AspS9I | GGNCC | 1 cut(s) | 178 |
| AsuHPI | GGTGA | 3 cut(s) | 27, 128, 343 |
| BccI | CCATC | 1 cut(s) | 67 |
| BciT130I | CCWGG | 1 cut(s) | 114 |
| BcoDI | GTCTC | 1 cut(s) | 102 |
| BfaI | CTAG | 1 cut(s) | 153 |
| Bme1390I | CCNGG | 1 cut(s) | 114 |
| BmgBI | CACGTC | 1 cut(s) | 110 |
| BmgT120I | GGNCC | 1 cut(s) | 178 |
| BmiI | GGNNCC | 1 cut(s) | 180 |
| BmrFI | CCNGG | 1 cut(s) | 114 |
| BmsI | GCATC | 1 cut(s) | 291 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 17, 49 |
| BpuEI | CTTGAG | 1 cut(s) | 213 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 124, 154 |
| BseBI | CCWGG | 1 cut(s) | 114 |
| BseGI | GGATG | 2 cut(s) | 282, 294 |
| BseMII | CTCAG | 1 cut(s) | 197 |
| BseRI | GAGGAG | 3 cut(s) | 126, 129, 428 |
| BshFI | GGCC | 1 cut(s) | 179 |
| BsiSI | CCGG | 1 cut(s) | 234 |
| BsmAI | GTCTC | 1 cut(s) | 102 |
| BsnI | GGCC | 1 cut(s) | 179 |
| BspACI | CCGC | 1 cut(s) | 298 |
| BspANI | GGCC | 1 cut(s) | 179 |
| BspCNI | CTCAG | 1 cut(s) | 196 |
| BspLI | GGNNCC | 1 cut(s) | 180 |
| Bst2UI | CCWGG | 1 cut(s) | 114 |
| BstC8I | GCNNGC | 2 cut(s) | 206, 278 |
| BstDEI | CTNAG | 1 cut(s) | 183 |
| BstF5I | GGATG | 2 cut(s) | 282, 294 |
| BstHHI | GCGC | 1 cut(s) | 282 |
| BstMAI | GTCTC | 1 cut(s) | 102 |
| BstNI | CCWGG | 1 cut(s) | 114 |
| BstSCI | CCNGG | 1 cut(s) | 112 |
| BsuRI | GGCC | 1 cut(s) | 179 |
| BtrI | CACGTC | 1 cut(s) | 110 |
| BtsCI | GGATG | 2 cut(s) | 282, 294 |
| Cac8I | GCNNGC | 2 cut(s) | 206, 278 |
| CfoI | GCGC | 1 cut(s) | 282 |
| Cfr13I | GGNCC | 1 cut(s) | 178 |
| CseI | GACGC | 1 cut(s) | 400 |
| CviAII | CATG | 1 cut(s) | 428 |
| CviJI | RGCY | 4 cut(s) | 32, 179, 192, 233 |
| CviKI_1 | RGCY | 4 cut(s) | 32, 179, 192, 233 |
| DdeI | CTNAG | 1 cut(s) | 183 |
| EcoO109I | RGGNCCY | 1 cut(s) | 178 |
| EcoRI | GAATTC | 1 cut(s) | 237 |
| EcoRII | CCWGG | 1 cut(s) | 112 |
| FaeI | CATG | 1 cut(s) | 431 |
| FaiI | YATR | 4 cut(s) | 68, 93, 396, 429 |
| FatI | CATG | 1 cut(s) | 427 |
| FokI | GGATG | 2 cut(s) | 269, 281 |
| FspBI | CTAG | 1 cut(s) | 153 |
| GlaI | GCGC | 1 cut(s) | 281 |
| HaeIII | GGCC | 1 cut(s) | 179 |
| HapII | CCGG | 1 cut(s) | 234 |
| HgaI | GACGC | 1 cut(s) | 400 |
| HhaI | GCGC | 1 cut(s) | 282 |
| Hin1II | CATG | 1 cut(s) | 431 |
| Hin6I | GCGC | 1 cut(s) | 280 |
| HinP1I | GCGC | 1 cut(s) | 280 |
| HinfI | GANTC | 2 cut(s) | 95, 309 |
| HpaII | CCGG | 1 cut(s) | 234 |
| HphI | GGTGA | 3 cut(s) | 27, 128, 343 |
| Hpy188I | TCNGA | 3 cut(s) | 186, 243, 287 |
| Hpy99I | CGWCG | 2 cut(s) | 394, 413 |
| HpyCH4IV | ACGT | 1 cut(s) | 109 |
| HpyF3I | CTNAG | 1 cut(s) | 183 |
| HpySE526I | ACGT | 1 cut(s) | 109 |
| Hsp92II | CATG | 1 cut(s) | 431 |
| HspAI | GCGC | 1 cut(s) | 280 |
| LpnPI | CCDG | 3 cut(s) | 99, 126, 247 |
| LweI | GCATC | 1 cut(s) | 291 |
| MaeI | CTAG | 1 cut(s) | 153 |
| MaeII | ACGT | 1 cut(s) | 109 |
| MfeI | CAATTG | 1 cut(s) | 56 |
| MluCI | AATT | 3 cut(s) | 56, 237, 476 |
| MlyI | GAGTC | 2 cut(s) | 104, 303 |
| MseI | TTAA | 2 cut(s) | 42, 441 |
| MslI | CAYNNNNRTG | 1 cut(s) | 71 |
| MspI | CCGG | 1 cut(s) | 234 |
| MspR9I | CCNGG | 1 cut(s) | 114 |
| MunI | CAATTG | 1 cut(s) | 56 |
| MvaI | CCWGG | 1 cut(s) | 114 |
| NlaIII | CATG | 1 cut(s) | 431 |
| NlaIV | GGNNCC | 1 cut(s) | 180 |
| PleI | GAGTC | 2 cut(s) | 103, 303 |
| PpsI | GAGTC | 2 cut(s) | 103, 303 |
| PshBI | ATTAAT | 1 cut(s) | 441 |
| Psp6I | CCWGG | 1 cut(s) | 112 |
| PspGI | CCWGG | 1 cut(s) | 112 |
| PspN4I | GGNNCC | 1 cut(s) | 180 |
| PspPI | GGNCC | 1 cut(s) | 178 |
| RseI | CAYNNNNRTG | 1 cut(s) | 71 |
| SaqAI | TTAA | 2 cut(s) | 42, 441 |
| Sau96I | GGNCC | 1 cut(s) | 178 |
| SchI | GAGTC | 2 cut(s) | 104, 303 |
| ScrFI | CCNGG | 1 cut(s) | 114 |
| SetI | ASST | 5 cut(s) | 15, 112, 118, 154, 261 |
| SfaNI | GCATC | 1 cut(s) | 291 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 71 |
| SmlI | CTYRAG | 1 cut(s) | 228 |
| SmoI | CTYRAG | 1 cut(s) | 228 |
| Sse9I | AATT | 3 cut(s) | 56, 237, 476 |
| SsiI | CCGC | 1 cut(s) | 298 |
| SspMI | CTAG | 1 cut(s) | 153 |
| StyD4I | CCNGG | 1 cut(s) | 112 |
| TaiI | ACGT | 1 cut(s) | 112 |
| TaqI | TCGA | 1 cut(s) | 447 |
| TaqII | GACCGA | 1 cut(s) | 391 |
| TasI | AATT | 3 cut(s) | 56, 237, 476 |
| Tru1I | TTAA | 2 cut(s) | 42, 441 |
| Tru9I | TTAA | 2 cut(s) | 42, 441 |
| TspGWI | ACGGA | 1 cut(s) | 334 |
| VspI | ATTAAT | 1 cut(s) | 441 |
| XapI | RAATTY | 1 cut(s) | 237 |
| XspI | CTAG | 1 cut(s) | 153 |