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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 423 |
| AcsI | RAATTY | 1 cut(s) | 399 |
| AgsI | TTSAA | 2 cut(s) | 97, 505 |
| AjiI | CACGTC | 1 cut(s) | 272 |
| AjnI | CCWGG | 1 cut(s) | 274 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 111, 143 |
| AluBI | AGCT | 1 cut(s) | 64 |
| AluI | AGCT | 1 cut(s) | 64 |
| Alw26I | GTCTC | 2 cut(s) | 7, 264 |
| Ama87I | CYCGRG | 1 cut(s) | 345 |
| AoxI | GGCC | 1 cut(s) | 339 |
| ApoI | RAATTY | 1 cut(s) | 399 |
| AspLEI | GCGC | 1 cut(s) | 443 |
| AspS9I | GGNCC | 1 cut(s) | 340 |
| AsuHPI | GGTGA | 3 cut(s) | 189, 290, 488 |
| AvaI | CYCGRG | 1 cut(s) | 345 |
| BbsI | GAAGAC | 1 cut(s) | 35 |
| BccI | CCATC | 3 cut(s) | 3, 142, 229 |
| BciT130I | CCWGG | 1 cut(s) | 276 |
| BcoDI | GTCTC | 2 cut(s) | 7, 264 |
| BfaI | CTAG | 1 cut(s) | 315 |
| Bme1390I | CCNGG | 1 cut(s) | 276 |
| BmeT110I | CYCGRG | 1 cut(s) | 345 |
| BmgBI | CACGTC | 1 cut(s) | 272 |
| BmgT120I | GGNCC | 1 cut(s) | 340 |
| BmiI | GGNNCC | 1 cut(s) | 342 |
| BmrFI | CCNGG | 1 cut(s) | 276 |
| BmsI | GCATC | 1 cut(s) | 452 |
| BoxI | GACNNNNGTC | 1 cut(s) | 492 |
| BpiI | GAAGAC | 1 cut(s) | 35 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 179, 211 |
| BpmI | CTGGAG | 1 cut(s) | 86 |
| BpuEI | CTTGAG | 1 cut(s) | 375 |
| BsaJI | CCNNGG | 2 cut(s) | 344, 483 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 286, 316 |
| BseBI | CCWGG | 1 cut(s) | 276 |
| BseDI | CCNNGG | 2 cut(s) | 344, 483 |
| BseGI | GGATG | 2 cut(s) | 160, 443 |
| BseRI | GAGGAG | 2 cut(s) | 288, 291 |
| BshFI | GGCC | 1 cut(s) | 341 |
| BsiHKCI | CYCGRG | 1 cut(s) | 345 |
| BsiSI | CCGG | 1 cut(s) | 396 |
| BslFI | GGGAC | 1 cut(s) | 362 |
| BsmAI | GTCTC | 2 cut(s) | 7, 264 |
| BsmBI | CGTCTC | 1 cut(s) | 7 |
| BsmFI | GGGAC | 1 cut(s) | 362 |
| BsmI | GAATGC | 1 cut(s) | 511 |
| BsnI | GGCC | 1 cut(s) | 341 |
| BsoBI | CYCGRG | 1 cut(s) | 345 |
| BspACI | CCGC | 1 cut(s) | 423 |
| BspANI | GGCC | 1 cut(s) | 341 |
| BspLI | GGNNCC | 1 cut(s) | 342 |
| BssECI | CCNNGG | 2 cut(s) | 344, 483 |
| Bst2UI | CCWGG | 1 cut(s) | 276 |
| Bst6I | CTCTTC | 3 cut(s) | 33, 75, 138 |
| BstC8I | GCNNGC | 2 cut(s) | 368, 439 |
| BstEII | GGTNACC | 1 cut(s) | 494 |
| BstF5I | GGATG | 2 cut(s) | 160, 443 |
| BstHHI | GCGC | 1 cut(s) | 443 |
| BstMAI | GTCTC | 2 cut(s) | 7, 264 |
| BstNI | CCWGG | 1 cut(s) | 276 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 492 |
| BstPI | GGTNACC | 1 cut(s) | 494 |
| BstSCI | CCNGG | 1 cut(s) | 274 |
| BstV2I | GAAGAC | 1 cut(s) | 35 |
| BsuRI | GGCC | 1 cut(s) | 341 |
| BtrI | CACGTC | 1 cut(s) | 272 |
| BtsCI | GGATG | 2 cut(s) | 160, 443 |
| Cac8I | GCNNGC | 2 cut(s) | 368, 439 |
| CfoI | GCGC | 1 cut(s) | 443 |
| Cfr13I | GGNCC | 1 cut(s) | 340 |
| CviJI | RGCY | 5 cut(s) | 64, 194, 341, 355, 395 |
| CviKI_1 | RGCY | 5 cut(s) | 64, 194, 341, 355, 395 |
| Eam1104I | CTCTTC | 3 cut(s) | 33, 75, 138 |
| EarI | CTCTTC | 3 cut(s) | 33, 75, 138 |
| Eco88I | CYCGRG | 1 cut(s) | 345 |
| Eco91I | GGTNACC | 1 cut(s) | 494 |
| EcoO109I | RGGNCCY | 1 cut(s) | 340 |
| EcoO65I | GGTNACC | 1 cut(s) | 494 |
| EcoRI | GAATTC | 1 cut(s) | 399 |
| EcoRII | CCWGG | 1 cut(s) | 274 |
| Esp3I | CGTCTC | 1 cut(s) | 7 |
| FaiI | YATR | 2 cut(s) | 230, 255 |
| FaqI | GGGAC | 1 cut(s) | 362 |
| FauI | CCCGC | 1 cut(s) | 430 |
| FokI | GGATG | 2 cut(s) | 167, 430 |
| FspBI | CTAG | 1 cut(s) | 315 |
| GlaI | GCGC | 1 cut(s) | 442 |
| GsuI | CTGGAG | 1 cut(s) | 86 |
| HaeIII | GGCC | 1 cut(s) | 341 |
| HapII | CCGG | 1 cut(s) | 396 |
| HhaI | GCGC | 1 cut(s) | 443 |
| Hin6I | GCGC | 1 cut(s) | 441 |
| HinP1I | GCGC | 1 cut(s) | 441 |
| HinfI | GANTC | 2 cut(s) | 257, 472 |
| HpaII | CCGG | 1 cut(s) | 396 |
| HphI | GGTGA | 3 cut(s) | 189, 290, 488 |
| Hpy188I | TCNGA | 2 cut(s) | 448, 471 |
| Hpy188III | TCNNGA | 3 cut(s) | 110, 347, 404 |
| HpyCH4IV | ACGT | 1 cut(s) | 271 |
| HpySE526I | ACGT | 1 cut(s) | 271 |
| HspAI | GCGC | 1 cut(s) | 441 |
| LpnPI | CCDG | 5 cut(s) | 50, 261, 288, 389, 409 |
| LweI | GCATC | 1 cut(s) | 452 |
| MaeI | CTAG | 1 cut(s) | 315 |
| MaeII | ACGT | 1 cut(s) | 271 |
| MaeIII | GTNAC | 1 cut(s) | 494 |
| MboII | GAAGA | 7 cut(s) | 18, 40, 50, 55, 89, 92, 155 |
| MfeI | CAATTG | 1 cut(s) | 218 |
| MluCI | AATT | 4 cut(s) | 50, 126, 218, 399 |
| MlyI | GAGTC | 2 cut(s) | 266, 466 |
| MmeI | TCCRAC | 1 cut(s) | 494 |
| MseI | TTAA | 1 cut(s) | 204 |
| MslI | CAYNNNNRTG | 2 cut(s) | 233, 504 |
| MspI | CCGG | 1 cut(s) | 396 |
| MspR9I | CCNGG | 1 cut(s) | 276 |
| MunI | CAATTG | 1 cut(s) | 218 |
| Mva1269I | GAATGC | 1 cut(s) | 511 |
| MvaI | CCWGG | 1 cut(s) | 276 |
| NlaIV | GGNNCC | 1 cut(s) | 342 |
| NmuCI | GTSAC | 1 cut(s) | 494 |
| PctI | GAATGC | 1 cut(s) | 511 |
| PleI | GAGTC | 2 cut(s) | 265, 466 |
| PpsI | GAGTC | 2 cut(s) | 265, 466 |
| PshAI | GACNNNNGTC | 1 cut(s) | 492 |
| Psp6I | CCWGG | 1 cut(s) | 274 |
| PspEI | GGTNACC | 1 cut(s) | 494 |
| PspGI | CCWGG | 1 cut(s) | 274 |
| PspN4I | GGNNCC | 1 cut(s) | 342 |
| PspPI | GGNCC | 1 cut(s) | 340 |
| RseI | CAYNNNNRTG | 2 cut(s) | 233, 504 |
| SaqAI | TTAA | 1 cut(s) | 204 |
| Sau96I | GGNCC | 1 cut(s) | 340 |
| SchI | GAGTC | 2 cut(s) | 266, 466 |
| ScrFI | CCNGG | 1 cut(s) | 276 |
| SetI | ASST | 5 cut(s) | 66, 177, 274, 280, 316 |
| SfaNI | GCATC | 1 cut(s) | 452 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 233, 504 |
| SmlI | CTYRAG | 1 cut(s) | 390 |
| SmoI | CTYRAG | 1 cut(s) | 390 |
| Sse9I | AATT | 4 cut(s) | 50, 126, 218, 399 |
| SsiI | CCGC | 1 cut(s) | 423 |
| SspMI | CTAG | 1 cut(s) | 315 |
| StyD4I | CCNGG | 1 cut(s) | 274 |
| TaiI | ACGT | 1 cut(s) | 274 |
| TaqII | GACCGA | 1 cut(s) | 482 |
| TasI | AATT | 4 cut(s) | 50, 126, 218, 399 |
| Tru1I | TTAA | 1 cut(s) | 204 |
| Tru9I | TTAA | 1 cut(s) | 204 |
| TseFI | GTSAC | 1 cut(s) | 494 |
| Tsp45I | GTSAC | 1 cut(s) | 494 |
| TspDTI | ATGAA | 2 cut(s) | 51, 63 |
| XapI | RAATTY | 1 cut(s) | 399 |
| XspI | CTAG | 1 cut(s) | 315 |