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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 345 |
| AciI | CCGC | 2 cut(s) | 140, 425 |
| AclWI | GGATC | 1 cut(s) | 422 |
| AcsI | RAATTY | 1 cut(s) | 286 |
| AfaI | GTAC | 2 cut(s) | 31, 348 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 335, 363 |
| AgsI | TTSAA | 2 cut(s) | 189, 445 |
| AjuI | GAANNNNNNNTTGG | 1 cut(s) | 437 |
| AluBI | AGCT | 3 cut(s) | 136, 245, 440 |
| AluI | AGCT | 3 cut(s) | 136, 245, 440 |
| AlwI | GGATC | 1 cut(s) | 422 |
| ApeKI | GCWGC | 2 cut(s) | 136, 428 |
| ApoI | RAATTY | 1 cut(s) | 286 |
| AspS9I | GGNCC | 1 cut(s) | 262 |
| AsuC2I | CCSGG | 1 cut(s) | 336 |
| AsuII | TTCGAA | 1 cut(s) | 290 |
| AvaII | GGWCC | 1 cut(s) | 262 |
| BbsI | GAAGAC | 1 cut(s) | 20 |
| BbvI | GCAGC | 2 cut(s) | 123, 415 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 195, 229 |
| BcnI | CCSGG | 1 cut(s) | 336 |
| BfmI | CTRYAG | 1 cut(s) | 429 |
| BglII | AGATCT | 1 cut(s) | 374 |
| BisI | GCNGC | 4 cut(s) | 137, 140, 426, 429 |
| BlsI | GCNGC | 4 cut(s) | 138, 141, 427, 430 |
| Bme1390I | CCNGG | 1 cut(s) | 336 |
| Bme18I | GGWCC | 1 cut(s) | 262 |
| BmgT120I | GGNCC | 1 cut(s) | 262 |
| BmrFI | CCNGG | 1 cut(s) | 336 |
| BmsI | GCATC | 2 cut(s) | 160, 193 |
| BpiI | GAAGAC | 1 cut(s) | 20 |
| Bpu14I | TTCGAA | 1 cut(s) | 290 |
| BpuMI | CCSGG | 1 cut(s) | 336 |
| BsaJI | CCNNGG | 1 cut(s) | 178 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 335, 363 |
| BseDI | CCNNGG | 1 cut(s) | 178 |
| BseGI | GGATG | 3 cut(s) | 79, 208, 223 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 335, 363 |
| BseMII | CTCAG | 1 cut(s) | 369 |
| BseXI | GCAGC | 2 cut(s) | 123, 415 |
| BseYI | CCCAGC | 1 cut(s) | 94 |
| Bsh1236I | CGCG | 1 cut(s) | 345 |
| BsiSI | CCGG | 1 cut(s) | 335 |
| BslFI | GGGAC | 2 cut(s) | 77, 139 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 335, 363 |
| BsmFI | GGGAC | 2 cut(s) | 77, 139 |
| BsmI | GAATGC | 1 cut(s) | 173 |
| Bsp119I | TTCGAA | 1 cut(s) | 290 |
| Bsp143I | GATC | 2 cut(s) | 374, 414 |
| Bsp19I | CCATGG | 1 cut(s) | 178 |
| BspACI | CCGC | 2 cut(s) | 140, 425 |
| BspCNI | CTCAG | 1 cut(s) | 370 |
| BspFNI | CGCG | 1 cut(s) | 345 |
| BspMAI | CTGCAG | 1 cut(s) | 433 |
| BspPI | GGATC | 1 cut(s) | 422 |
| BspT104I | TTCGAA | 1 cut(s) | 290 |
| BssECI | CCNNGG | 1 cut(s) | 178 |
| BssMI | GATC | 2 cut(s) | 374, 414 |
| BssT1I | CCWWGG | 1 cut(s) | 178 |
| Bst6I | CTCTTC | 2 cut(s) | 75, 95 |
| BstBI | TTCGAA | 1 cut(s) | 290 |
| BstDEI | CTNAG | 1 cut(s) | 378 |
| BstDSI | CCRYGG | 1 cut(s) | 178 |
| BstF5I | GGATG | 3 cut(s) | 79, 208, 223 |
| BstFNI | CGCG | 1 cut(s) | 345 |
| BstKTI | GATC | 2 cut(s) | 377, 417 |
| BstMBI | GATC | 2 cut(s) | 374, 414 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 437 |
| BstSCI | CCNGG | 1 cut(s) | 334 |
| BstSFI | CTRYAG | 1 cut(s) | 429 |
| BstUI | CGCG | 1 cut(s) | 345 |
| BstV1I | GCAGC | 2 cut(s) | 123, 415 |
| BstV2I | GAAGAC | 1 cut(s) | 20 |
| BstX2I | RGATCY | 1 cut(s) | 374 |
| BstYI | RGATCY | 1 cut(s) | 374 |
| BtgI | CCRYGG | 1 cut(s) | 178 |
| BtsCI | GGATG | 3 cut(s) | 79, 208, 223 |
| Cfr13I | GGNCC | 1 cut(s) | 262 |
| Csp6I | GTAC | 2 cut(s) | 30, 347 |
| CviAII | CATG | 1 cut(s) | 179 |
| CviJI | RGCY | 4 cut(s) | 136, 245, 428, 440 |
| CviKI_1 | RGCY | 4 cut(s) | 136, 245, 428, 440 |
| CviQI | GTAC | 2 cut(s) | 30, 347 |
| DdeI | CTNAG | 1 cut(s) | 378 |
| DpnI | GATC | 2 cut(s) | 376, 416 |
| DpnII | GATC | 2 cut(s) | 374, 414 |
| Eam1104I | CTCTTC | 2 cut(s) | 75, 95 |
| EarI | CTCTTC | 2 cut(s) | 75, 95 |
| Eco130I | CCWWGG | 1 cut(s) | 178 |
| Eco47I | GGWCC | 1 cut(s) | 262 |
| EcoT14I | CCWWGG | 1 cut(s) | 178 |
| EcoT22I | ATGCAT | 1 cut(s) | 175 |
| ErhI | CCWWGG | 1 cut(s) | 178 |
| FaeI | CATG | 1 cut(s) | 182 |
| FaiI | YATR | 5 cut(s) | 114, 180, 308, 314, 370 |
| FaqI | GGGAC | 2 cut(s) | 77, 139 |
| FatI | CATG | 1 cut(s) | 178 |
| Fnu4HI | GCNGC | 4 cut(s) | 137, 140, 426, 429 |
| FokI | GGATG | 3 cut(s) | 86, 215, 230 |
| Fsp4HI | GCNGC | 4 cut(s) | 137, 140, 426, 429 |
| GluI | GCNGC | 4 cut(s) | 137, 140, 426, 429 |
| GsaI | CCCAGC | 1 cut(s) | 98 |
| HapII | CCGG | 1 cut(s) | 335 |
| Hin1II | CATG | 1 cut(s) | 182 |
| HindIII | AAGCTT | 2 cut(s) | 243, 438 |
| HinfI | GANTC | 1 cut(s) | 331 |
| HpaII | CCGG | 1 cut(s) | 335 |
| Hpy188I | TCNGA | 2 cut(s) | 217, 379 |
| Hpy188III | TCNNGA | 1 cut(s) | 385 |
| Hpy99I | CGWCG | 1 cut(s) | 56 |
| HpyAV | CCTTC | 3 cut(s) | 34, 356, 442 |
| HpyCH4V | TGCA | 4 cut(s) | 158, 173, 206, 431 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 437 |
| HpyF3I | CTNAG | 1 cut(s) | 378 |
| Hsp92II | CATG | 1 cut(s) | 182 |
| Kzo9I | GATC | 2 cut(s) | 374, 414 |
| LpnPI | CCDG | 2 cut(s) | 108, 348 |
| Lsp1109I | GCAGC | 2 cut(s) | 123, 415 |
| LweI | GCATC | 2 cut(s) | 160, 193 |
| MaeIII | GTNAC | 1 cut(s) | 385 |
| MalI | GATC | 2 cut(s) | 376, 416 |
| MboI | GATC | 2 cut(s) | 374, 414 |
| MboII | GAAGA | 8 cut(s) | 25, 49, 56, 71, 74, 92, 112, 457 |
| MflI | RGATCY | 1 cut(s) | 374 |
| MluCI | AATT | 2 cut(s) | 189, 286 |
| MnlI | CCTC | 6 cut(s) | 49, 76, 115, 176, 405, 427 |
| Mph1103I | ATGCAT | 1 cut(s) | 175 |
| MseI | TTAA | 2 cut(s) | 192, 224 |
| MspI | CCGG | 1 cut(s) | 335 |
| MspR9I | CCNGG | 1 cut(s) | 336 |
| Mva1269I | GAATGC | 1 cut(s) | 173 |
| MvnI | CGCG | 1 cut(s) | 345 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 437 |
| NciI | CCSGG | 1 cut(s) | 336 |
| NcoI | CCATGG | 1 cut(s) | 178 |
| NdeII | GATC | 2 cut(s) | 374, 414 |
| NlaIII | CATG | 1 cut(s) | 182 |
| NmuCI | GTSAC | 1 cut(s) | 385 |
| NsiI | ATGCAT | 1 cut(s) | 175 |
| NspV | TTCGAA | 1 cut(s) | 290 |
| PctI | GAATGC | 1 cut(s) | 173 |
| PfeI | GAWTC | 1 cut(s) | 331 |
| PkrI | GCNGC | 4 cut(s) | 138, 141, 427, 430 |
| PspFI | CCCAGC | 1 cut(s) | 94 |
| PspPI | GGNCC | 1 cut(s) | 262 |
| PstI | CTGCAG | 1 cut(s) | 433 |
| PsuI | RGATCY | 1 cut(s) | 374 |
| RsaI | GTAC | 2 cut(s) | 31, 348 |
| RsaNI | GTAC | 2 cut(s) | 30, 347 |
| SaqAI | TTAA | 2 cut(s) | 192, 224 |
| SatI | GCNGC | 4 cut(s) | 137, 140, 426, 429 |
| Sau3AI | GATC | 2 cut(s) | 374, 414 |
| Sau96I | GGNCC | 1 cut(s) | 262 |
| ScrFI | CCNGG | 1 cut(s) | 336 |
| SetI | ASST | 5 cut(s) | 138, 187, 247, 267, 442 |
| SfaNI | GCATC | 2 cut(s) | 160, 193 |
| SfcI | CTRYAG | 1 cut(s) | 429 |
| SfuI | TTCGAA | 1 cut(s) | 290 |
| SinI | GGWCC | 1 cut(s) | 262 |
| Sse9I | AATT | 2 cut(s) | 189, 286 |
| SsiI | CCGC | 2 cut(s) | 140, 425 |
| StyD4I | CCNGG | 1 cut(s) | 334 |
| StyI | CCWWGG | 1 cut(s) | 178 |
| TaqI | TCGA | 1 cut(s) | 290 |
| TasI | AATT | 2 cut(s) | 189, 286 |
| TatI | WGTACW | 1 cut(s) | 29 |
| TauI | GCSGC | 2 cut(s) | 142, 428 |
| TfiI | GAWTC | 1 cut(s) | 331 |
| Tru1I | TTAA | 2 cut(s) | 192, 224 |
| Tru9I | TTAA | 2 cut(s) | 192, 224 |
| TseFI | GTSAC | 1 cut(s) | 385 |
| TseI | GCWGC | 2 cut(s) | 136, 428 |
| Tsp45I | GTSAC | 1 cut(s) | 385 |
| TspDTI | ATGAA | 1 cut(s) | 93 |
| TspGWI | ACGGA | 1 cut(s) | 275 |
| VpaK11BI | GGWCC | 1 cut(s) | 262 |
| XapI | RAATTY | 1 cut(s) | 286 |
| Zsp2I | ATGCAT | 1 cut(s) | 175 |