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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 2 cut(s) | 584, 595 |
| AcsI | RAATTY | 2 cut(s) | 569, 883 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 523, 620, 842 |
| AgsI | TTSAA | 3 cut(s) | 597, 687, 959 |
| AluBI | AGCT | 2 cut(s) | 725, 930 |
| AluI | AGCT | 2 cut(s) | 725, 930 |
| AlwI | GGATC | 2 cut(s) | 584, 595 |
| ApeKI | GCWGC | 1 cut(s) | 979 |
| ApoI | RAATTY | 2 cut(s) | 569, 883 |
| AseI | ATTAAT | 1 cut(s) | 800 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 569, 853 |
| AspA2I | CCTAGG | 1 cut(s) | 604 |
| AspLEI | GCGC | 1 cut(s) | 739 |
| AspS9I | GGNCC | 1 cut(s) | 631 |
| AsuHPI | GGTGA | 2 cut(s) | 423, 635 |
| AvaII | GGWCC | 1 cut(s) | 631 |
| AvrII | CCTAGG | 1 cut(s) | 604 |
| BceAI | ACGGC | 1 cut(s) | 698 |
| BclI | TGATCA | 5 cut(s) | 16, 28, 139, 250, 361 |
| BfaI | CTAG | 1 cut(s) | 605 |
| BfmI | CTRYAG | 1 cut(s) | 521 |
| BglII | AGATCT | 1 cut(s) | 899 |
| BisI | GCNGC | 1 cut(s) | 980 |
| BlnI | CCTAGG | 1 cut(s) | 604 |
| BlsI | GCNGC | 1 cut(s) | 981 |
| Bme18I | GGWCC | 1 cut(s) | 631 |
| BmgT120I | GGNCC | 1 cut(s) | 631 |
| BmiI | GGNNCC | 1 cut(s) | 633 |
| BmrI | ACTGGG | 1 cut(s) | 919 |
| BmsI | GCATC | 2 cut(s) | 442, 724 |
| BmuI | ACTGGG | 1 cut(s) | 919 |
| BsaAI | YACGTR | 1 cut(s) | 868 |
| BsaJI | CCNNGG | 3 cut(s) | 460, 604, 714 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 523, 620, 842 |
| BseDI | CCNNGG | 3 cut(s) | 460, 604, 714 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 523, 620, 842 |
| BseMII | CTCAG | 1 cut(s) | 636 |
| BseRI | GAGGAG | 2 cut(s) | 491, 506 |
| BslFI | GGGAC | 2 cut(s) | 617, 875 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 523, 620, 842 |
| BsmFI | GGGAC | 2 cut(s) | 617, 875 |
| BsmI | GAATGC | 3 cut(s) | 455, 615, 754 |
| Bsp143I | GATC | 9 cut(s) | 16, 28, 139, 250, 361, 576, 600, 894, 899 |
| Bsp19I | CCATGG | 1 cut(s) | 460 |
| BspCNI | CTCAG | 1 cut(s) | 637 |
| BspLI | GGNNCC | 1 cut(s) | 633 |
| BspPI | GGATC | 2 cut(s) | 584, 595 |
| BssECI | CCNNGG | 3 cut(s) | 460, 604, 714 |
| BssMI | GATC | 9 cut(s) | 16, 28, 139, 250, 361, 576, 600, 894, 899 |
| BssT1I | CCWWGG | 2 cut(s) | 460, 604 |
| Bst4CI | ACNGT | 3 cut(s) | 112, 223, 334 |
| Bst6I | CTCTTC | 1 cut(s) | 554 |
| BstBAI | YACGTR | 1 cut(s) | 868 |
| BstC8I | GCNNGC | 1 cut(s) | 877 |
| BstDEI | CTNAG | 1 cut(s) | 645 |
| BstDSI | CCRYGG | 2 cut(s) | 460, 714 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 521 |
| BstHHI | GCGC | 1 cut(s) | 739 |
| BstKTI | GATC | 9 cut(s) | 19, 31, 142, 253, 364, 579, 603, 897, 902 |
| BstMBI | GATC | 9 cut(s) | 16, 28, 139, 250, 361, 576, 600, 894, 899 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 736 |
| BstNSI | RCATGY | 8 cut(s) | 130, 134, 241, 245, 352, 356, 391, 395 |
| BstSFI | CTRYAG | 1 cut(s) | 521 |
| BstSNI | TACGTA | 1 cut(s) | 868 |
| BstX2I | RGATCY | 3 cut(s) | 576, 600, 899 |
| BstXI | CCANNNNNNTGG | 7 cut(s) | 50, 74, 98, 161, 185, 272, 296 |
| BstYI | RGATCY | 3 cut(s) | 576, 600, 899 |
| BtgI | CCRYGG | 2 cut(s) | 460, 714 |
| BtsIMutI | CAGTG | 1 cut(s) | 19 |
| Cac8I | GCNNGC | 1 cut(s) | 877 |
| CfoI | GCGC | 1 cut(s) | 739 |
| Cfr13I | GGNCC | 1 cut(s) | 631 |
| DdeI | CTNAG | 1 cut(s) | 645 |
| DpnI | GATC | 9 cut(s) | 18, 30, 141, 252, 363, 578, 602, 896, 901 |
| DpnII | GATC | 9 cut(s) | 16, 28, 139, 250, 361, 576, 600, 894, 899 |
| Eam1104I | CTCTTC | 1 cut(s) | 554 |
| EarI | CTCTTC | 1 cut(s) | 554 |
| Eco105I | TACGTA | 1 cut(s) | 868 |
| Eco130I | CCWWGG | 2 cut(s) | 460, 604 |
| Eco147I | AGGCCT | 1 cut(s) | 527 |
| Eco47I | GGWCC | 1 cut(s) | 631 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 521 |
| EcoT14I | CCWWGG | 2 cut(s) | 460, 604 |
| EcoT22I | ATGCAT | 5 cut(s) | 132, 243, 354, 393, 457 |
| ErhI | CCWWGG | 2 cut(s) | 460, 604 |
| FaqI | GGGAC | 2 cut(s) | 617, 875 |
| FbaI | TGATCA | 5 cut(s) | 16, 28, 139, 250, 361 |
| Fnu4HI | GCNGC | 1 cut(s) | 980 |
| Fsp4HI | GCNGC | 1 cut(s) | 980 |
| FspBI | CTAG | 1 cut(s) | 605 |
| GlaI | GCGC | 1 cut(s) | 738 |
| GluI | GCNGC | 1 cut(s) | 980 |
| HhaI | GCGC | 1 cut(s) | 739 |
| Hin6I | GCGC | 1 cut(s) | 737 |
| HinP1I | GCGC | 1 cut(s) | 737 |
| HindIII | AAGCTT | 1 cut(s) | 928 |
| HinfI | GANTC | 1 cut(s) | 972 |
| HphI | GGTGA | 2 cut(s) | 423, 635 |
| Hpy188I | TCNGA | 4 cut(s) | 499, 646, 664, 899 |
| Hpy188III | TCNNGA | 2 cut(s) | 574, 580 |
| HpyAV | CCTTC | 2 cut(s) | 758, 772 |
| HpyCH4III | ACNGT | 3 cut(s) | 112, 223, 334 |
| HpyCH4IV | ACGT | 1 cut(s) | 867 |
| HpyCH4V | TGCA | 8 cut(s) | 130, 241, 352, 391, 455, 730, 752, 842 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 736 |
| HpyF3I | CTNAG | 1 cut(s) | 645 |
| HpySE526I | ACGT | 1 cut(s) | 867 |
| HspAI | GCGC | 1 cut(s) | 737 |
| Ksp22I | TGATCA | 5 cut(s) | 16, 28, 139, 250, 361 |
| Kzo9I | GATC | 9 cut(s) | 16, 28, 139, 250, 361, 576, 600, 894, 899 |
| LweI | GCATC | 2 cut(s) | 442, 724 |
| MaeI | CTAG | 1 cut(s) | 605 |
| MaeII | ACGT | 1 cut(s) | 867 |
| MalI | GATC | 9 cut(s) | 18, 30, 141, 252, 363, 578, 602, 896, 901 |
| MboI | GATC | 9 cut(s) | 16, 28, 139, 250, 361, 576, 600, 894, 899 |
| MboII | GAAGA | 4 cut(s) | 541, 577, 773, 787 |
| MflI | RGATCY | 3 cut(s) | 576, 600, 899 |
| MluCI | AATT | 4 cut(s) | 569, 699, 852, 883 |
| MlyI | GAGTC | 1 cut(s) | 981 |
| MmeI | TCCRAC | 2 cut(s) | 687, 904 |
| MnlI | CCTC | 5 cut(s) | 469, 527, 557, 640, 839 |
| Mph1103I | ATGCAT | 5 cut(s) | 132, 243, 354, 393, 457 |
| MroXI | GAANNNNTTC | 2 cut(s) | 569, 853 |
| MseI | TTAA | 3 cut(s) | 702, 800, 935 |
| MslI | CAYNNNNRTG | 1 cut(s) | 540 |
| Mva1269I | GAATGC | 3 cut(s) | 455, 615, 754 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 736 |
| NcoI | CCATGG | 1 cut(s) | 460 |
| NdeII | GATC | 9 cut(s) | 16, 28, 139, 250, 361, 576, 600, 894, 899 |
| NlaIV | GGNNCC | 1 cut(s) | 633 |
| NsiI | ATGCAT | 5 cut(s) | 132, 243, 354, 393, 457 |
| NspI | RCATGY | 8 cut(s) | 130, 134, 241, 245, 352, 356, 391, 395 |
| PceI | AGGCCT | 1 cut(s) | 527 |
| PctI | GAATGC | 3 cut(s) | 455, 615, 754 |
| PdmI | GAANNNNTTC | 2 cut(s) | 569, 853 |
| PkrI | GCNGC | 1 cut(s) | 981 |
| PleI | GAGTC | 1 cut(s) | 980 |
| PpsI | GAGTC | 1 cut(s) | 980 |
| Ppu21I | YACGTR | 1 cut(s) | 868 |
| PshBI | ATTAAT | 1 cut(s) | 800 |
| PspN4I | GGNNCC | 1 cut(s) | 633 |
| PspPI | GGNCC | 1 cut(s) | 631 |
| PsuI | RGATCY | 3 cut(s) | 576, 600, 899 |
| RseI | CAYNNNNRTG | 1 cut(s) | 540 |
| SaqAI | TTAA | 3 cut(s) | 702, 800, 935 |
| SatI | GCNGC | 1 cut(s) | 980 |
| Sau3AI | GATC | 9 cut(s) | 16, 28, 139, 250, 361, 576, 600, 894, 899 |
| Sau96I | GGNCC | 1 cut(s) | 631 |
| SchI | GAGTC | 1 cut(s) | 981 |
| SetI | ASST | 5 cut(s) | 549, 625, 727, 870, 932 |
| SfaNI | GCATC | 2 cut(s) | 442, 724 |
| SfcI | CTRYAG | 1 cut(s) | 521 |
| SinI | GGWCC | 1 cut(s) | 631 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 540 |
| SnaBI | TACGTA | 1 cut(s) | 868 |
| Sse9I | AATT | 4 cut(s) | 569, 699, 852, 883 |
| SseBI | AGGCCT | 1 cut(s) | 527 |
| SspI | AATATT | 1 cut(s) | 445 |
| SspMI | CTAG | 1 cut(s) | 605 |
| StuI | AGGCCT | 1 cut(s) | 527 |
| StyI | CCWWGG | 2 cut(s) | 460, 604 |
| TaaI | ACNGT | 3 cut(s) | 112, 223, 334 |
| TaiI | ACGT | 1 cut(s) | 870 |
| TaqI | TCGA | 1 cut(s) | 581 |
| TasI | AATT | 4 cut(s) | 569, 699, 852, 883 |
| Tru1I | TTAA | 3 cut(s) | 702, 800, 935 |
| Tru9I | TTAA | 3 cut(s) | 702, 800, 935 |
| TscAI | CASTG | 1 cut(s) | 19 |
| TseI | GCWGC | 1 cut(s) | 979 |
| TspDTI | ATGAA | 5 cut(s) | 17, 496, 552, 808, 846 |
| TspGWI | ACGGA | 1 cut(s) | 984 |
| TspRI | CASTG | 1 cut(s) | 19 |
| VpaK11BI | GGWCC | 1 cut(s) | 631 |
| VspI | ATTAAT | 1 cut(s) | 800 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 521 |
| XapI | RAATTY | 2 cut(s) | 569, 883 |
| XceI | RCATGY | 8 cut(s) | 130, 134, 241, 245, 352, 356, 391, 395 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 434 |
| XmaJI | CCTAGG | 1 cut(s) | 604 |
| XmnI | GAANNNNTTC | 2 cut(s) | 569, 853 |
| XspI | CTAG | 1 cut(s) | 605 |
| Zsp2I | ATGCAT | 5 cut(s) | 132, 243, 354, 393, 457 |