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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 5, 671 |
| AclWI | GGATC | 1 cut(s) | 668 |
| AcsI | RAATTY | 1 cut(s) | 422 |
| AcuI | CTGAAG | 5 cut(s) | 72, 165, 297, 405, 549 |
| AfaI | GTAC | 2 cut(s) | 364, 594 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 609 |
| AgsI | TTSAA | 2 cut(s) | 194, 691 |
| AjnI | CCWGG | 1 cut(s) | 427 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 683, 715 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 294, 326 |
| AluBI | AGCT | 2 cut(s) | 544, 686 |
| AluI | AGCT | 2 cut(s) | 544, 686 |
| AlwI | GGATC | 1 cut(s) | 668 |
| ApeKI | GCWGC | 4 cut(s) | 474, 544, 547, 674 |
| ApoI | RAATTY | 1 cut(s) | 422 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 309 |
| AsuC2I | CCSGG | 1 cut(s) | 221 |
| BbsI | GAAGAC | 1 cut(s) | 236 |
| BbvI | GCAGC | 4 cut(s) | 461, 531, 534, 661 |
| BccI | CCATC | 1 cut(s) | 293 |
| BciT130I | CCWGG | 1 cut(s) | 429 |
| BcnI | CCSGG | 1 cut(s) | 221 |
| BfmI | CTRYAG | 2 cut(s) | 548, 675 |
| BisI | GCNGC | 5 cut(s) | 475, 545, 548, 672, 675 |
| BlsI | GCNGC | 5 cut(s) | 476, 546, 549, 673, 676 |
| Bme1390I | CCNGG | 2 cut(s) | 221, 429 |
| BmrFI | CCNGG | 2 cut(s) | 221, 429 |
| BmsI | GCATC | 1 cut(s) | 568 |
| BpiI | GAAGAC | 1 cut(s) | 236 |
| BpuMI | CCSGG | 1 cut(s) | 221 |
| BsaJI | CCNNGG | 4 cut(s) | 220, 427, 428, 586 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 294, 324 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 609 |
| BseBI | CCWGG | 1 cut(s) | 429 |
| BseDI | CCNNGG | 4 cut(s) | 220, 427, 428, 586 |
| BseGI | GGATG | 1 cut(s) | 412 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 609 |
| BseXI | GCAGC | 4 cut(s) | 461, 531, 534, 661 |
| BsgI | GTGCAG | 1 cut(s) | 460 |
| BsiSI | CCGG | 1 cut(s) | 220 |
| BslFI | GGGAC | 1 cut(s) | 547 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 609 |
| BsmFI | GGGAC | 1 cut(s) | 547 |
| BsmI | GAATGC | 2 cut(s) | 581, 700 |
| Bsp143I | GATC | 1 cut(s) | 660 |
| Bsp19I | CCATGG | 1 cut(s) | 586 |
| BspACI | CCGC | 2 cut(s) | 5, 671 |
| BspMAI | CTGCAG | 2 cut(s) | 552, 679 |
| BspPI | GGATC | 1 cut(s) | 668 |
| BssECI | CCNNGG | 4 cut(s) | 220, 427, 428, 586 |
| BssMI | GATC | 1 cut(s) | 660 |
| BssT1I | CCWWGG | 1 cut(s) | 586 |
| Bst2UI | CCWGG | 1 cut(s) | 429 |
| Bst6I | CTCTTC | 2 cut(s) | 381, 650 |
| BstDSI | CCRYGG | 1 cut(s) | 586 |
| BstF5I | GGATG | 1 cut(s) | 412 |
| BstKTI | GATC | 1 cut(s) | 663 |
| BstMBI | GATC | 1 cut(s) | 660 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 683 |
| BstNI | CCWGG | 1 cut(s) | 429 |
| BstSCI | CCNGG | 2 cut(s) | 219, 427 |
| BstSFI | CTRYAG | 2 cut(s) | 548, 675 |
| BstV1I | GCAGC | 4 cut(s) | 461, 531, 534, 661 |
| BstV2I | GAAGAC | 1 cut(s) | 236 |
| BtgI | CCRYGG | 1 cut(s) | 586 |
| BtsCI | GGATG | 1 cut(s) | 412 |
| Csp6I | GTAC | 2 cut(s) | 363, 593 |
| CviAII | CATG | 1 cut(s) | 587 |
| CviJI | RGCY | 3 cut(s) | 544, 674, 686 |
| CviKI_1 | RGCY | 3 cut(s) | 544, 674, 686 |
| CviQI | GTAC | 2 cut(s) | 363, 593 |
| DpnI | GATC | 1 cut(s) | 662 |
| DpnII | GATC | 1 cut(s) | 660 |
| Eam1104I | CTCTTC | 2 cut(s) | 381, 650 |
| EarI | CTCTTC | 2 cut(s) | 381, 650 |
| EciI | GGCGGA | 1 cut(s) | 20 |
| Eco130I | CCWWGG | 1 cut(s) | 586 |
| Eco57I | CTGAAG | 5 cut(s) | 72, 165, 297, 405, 549 |
| EcoRII | CCWGG | 1 cut(s) | 427 |
| EcoT14I | CCWWGG | 1 cut(s) | 586 |
| EcoT22I | ATGCAT | 2 cut(s) | 583, 700 |
| ErhI | CCWWGG | 1 cut(s) | 586 |
| FaeI | CATG | 1 cut(s) | 590 |
| FaiI | YATR | 5 cut(s) | 447, 462, 588, 616, 715 |
| FaqI | GGGAC | 1 cut(s) | 547 |
| FatI | CATG | 1 cut(s) | 586 |
| Fnu4HI | GCNGC | 5 cut(s) | 475, 545, 548, 672, 675 |
| FokI | GGATG | 1 cut(s) | 419 |
| Fsp4HI | GCNGC | 5 cut(s) | 475, 545, 548, 672, 675 |
| GluI | GCNGC | 5 cut(s) | 475, 545, 548, 672, 675 |
| HapII | CCGG | 1 cut(s) | 220 |
| Hin1II | CATG | 1 cut(s) | 590 |
| HindIII | AAGCTT | 1 cut(s) | 684 |
| HinfI | GANTC | 4 cut(s) | 32, 158, 200, 293 |
| HpaII | CCGG | 1 cut(s) | 220 |
| Hpy188I | TCNGA | 5 cut(s) | 52, 67, 298, 495, 625 |
| Hpy188III | TCNNGA | 1 cut(s) | 36 |
| HpyAV | CCTTC | 5 cut(s) | 232, 340, 367, 524, 602 |
| HpyCH4V | TGCA | 8 cut(s) | 466, 477, 506, 550, 566, 581, 677, 698 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 683 |
| Hsp92II | CATG | 1 cut(s) | 590 |
| Kzo9I | GATC | 1 cut(s) | 660 |
| LpnPI | CCDG | 7 cut(s) | 49, 233, 250, 327, 414, 441, 536 |
| Lsp1109I | GCAGC | 4 cut(s) | 461, 531, 534, 661 |
| LweI | GCATC | 1 cut(s) | 568 |
| MaeIII | GTNAC | 1 cut(s) | 631 |
| MalI | GATC | 1 cut(s) | 662 |
| MboI | GATC | 1 cut(s) | 660 |
| MfeI | CAATTG | 2 cut(s) | 75, 501 |
| MluCI | AATT | 3 cut(s) | 75, 422, 501 |
| MnlI | CCTC | 7 cut(s) | 22, 166, 382, 409, 448, 651, 673 |
| Mph1103I | ATGCAT | 2 cut(s) | 583, 700 |
| MroXI | GAANNNNTTC | 1 cut(s) | 309 |
| MseI | TTAA | 1 cut(s) | 89 |
| MspI | CCGG | 1 cut(s) | 220 |
| MspR9I | CCNGG | 2 cut(s) | 221, 429 |
| MunI | CAATTG | 2 cut(s) | 75, 501 |
| Mva1269I | GAATGC | 2 cut(s) | 581, 700 |
| MvaI | CCWGG | 1 cut(s) | 429 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 683 |
| NciI | CCSGG | 1 cut(s) | 221 |
| NcoI | CCATGG | 1 cut(s) | 586 |
| NdeII | GATC | 1 cut(s) | 660 |
| NlaIII | CATG | 1 cut(s) | 590 |
| NmuCI | GTSAC | 1 cut(s) | 631 |
| NsiI | ATGCAT | 2 cut(s) | 583, 700 |
| PasI | CCCWGGG | 1 cut(s) | 428 |
| PctI | GAATGC | 2 cut(s) | 581, 700 |
| PdmI | GAANNNNTTC | 1 cut(s) | 309 |
| PfeI | GAWTC | 4 cut(s) | 32, 158, 200, 293 |
| PkrI | GCNGC | 5 cut(s) | 476, 546, 549, 673, 676 |
| Psp6I | CCWGG | 1 cut(s) | 427 |
| PspGI | CCWGG | 1 cut(s) | 427 |
| PstI | CTGCAG | 2 cut(s) | 552, 679 |
| RsaI | GTAC | 2 cut(s) | 364, 594 |
| RsaNI | GTAC | 2 cut(s) | 363, 593 |
| SaqAI | TTAA | 1 cut(s) | 89 |
| SatI | GCNGC | 5 cut(s) | 475, 545, 548, 672, 675 |
| Sau3AI | GATC | 1 cut(s) | 660 |
| ScrFI | CCNGG | 2 cut(s) | 221, 429 |
| SetI | ASST | 3 cut(s) | 525, 546, 688 |
| SfaNI | GCATC | 1 cut(s) | 568 |
| SfcI | CTRYAG | 2 cut(s) | 548, 675 |
| Sse9I | AATT | 3 cut(s) | 75, 422, 501 |
| SsiI | CCGC | 2 cut(s) | 5, 671 |
| StyD4I | CCNGG | 2 cut(s) | 219, 427 |
| StyI | CCWWGG | 1 cut(s) | 586 |
| TasI | AATT | 3 cut(s) | 75, 422, 501 |
| TatI | WGTACW | 1 cut(s) | 362 |
| TauI | GCSGC | 1 cut(s) | 674 |
| TfiI | GAWTC | 4 cut(s) | 32, 158, 200, 293 |
| Tru1I | TTAA | 1 cut(s) | 89 |
| Tru9I | TTAA | 1 cut(s) | 89 |
| TseFI | GTSAC | 1 cut(s) | 631 |
| TseI | GCWGC | 4 cut(s) | 474, 544, 547, 674 |
| Tsp45I | GTSAC | 1 cut(s) | 631 |
| TspDTI | ATGAA | 3 cut(s) | 165, 222, 297 |
| TspGWI | ACGGA | 1 cut(s) | 118 |
| XapI | RAATTY | 1 cut(s) | 422 |
| XmnI | GAANNNNTTC | 1 cut(s) | 309 |
| Zsp2I | ATGCAT | 2 cut(s) | 583, 700 |