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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 337 |
| AciI | CCGC | 2 cut(s) | 218, 233 |
| AgsI | TTSAA | 3 cut(s) | 88, 311, 410 |
| AjiI | CACGTC | 1 cut(s) | 179 |
| AjnI | CCWGG | 1 cut(s) | 181 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 102, 134 |
| AluBI | AGCT | 2 cut(s) | 76, 428 |
| AluI | AGCT | 2 cut(s) | 76, 428 |
| AoxI | GGCC | 1 cut(s) | 246 |
| ApeKI | GCWGC | 3 cut(s) | 368, 425, 428 |
| AspS9I | GGNCC | 1 cut(s) | 247 |
| AsuHPI | GGTGA | 4 cut(s) | 159, 197, 393, 435 |
| AxyI | CCTNAGG | 2 cut(s) | 252, 456 |
| BbvI | GCAGC | 3 cut(s) | 380, 415, 437 |
| BciT130I | CCWGG | 1 cut(s) | 183 |
| BfaI | CTAG | 1 cut(s) | 222 |
| BfmI | CTRYAG | 1 cut(s) | 366 |
| BfuAI | ACCTGC | 1 cut(s) | 337 |
| BisI | GCNGC | 3 cut(s) | 369, 426, 429 |
| BlsI | GCNGC | 3 cut(s) | 370, 427, 430 |
| Bme1390I | CCNGG | 1 cut(s) | 183 |
| BmgBI | CACGTC | 1 cut(s) | 179 |
| BmgT120I | GGNCC | 1 cut(s) | 247 |
| BmiI | GGNNCC | 1 cut(s) | 249 |
| BmrFI | CCNGG | 1 cut(s) | 183 |
| BoxI | GACNNNNGTC | 1 cut(s) | 397 |
| BpuEI | CTTGAG | 1 cut(s) | 282 |
| BsaJI | CCNNGG | 1 cut(s) | 388 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 193, 223 |
| Bse21I | CCTNAGG | 2 cut(s) | 252, 456 |
| BseBI | CCWGG | 1 cut(s) | 183 |
| BseDI | CCNNGG | 1 cut(s) | 388 |
| BseGI | GGATG | 2 cut(s) | 151, 205 |
| BseMII | CTCAG | 3 cut(s) | 243, 345, 447 |
| BseRI | GAGGAG | 1 cut(s) | 116 |
| BseXI | GCAGC | 3 cut(s) | 380, 415, 437 |
| BsgI | GTGCAG | 1 cut(s) | 414 |
| Bsh1285I | CGRYCG | 1 cut(s) | 388 |
| BshFI | GGCC | 1 cut(s) | 248 |
| BsiEI | CGRYCG | 1 cut(s) | 388 |
| BsnI | GGCC | 1 cut(s) | 248 |
| BspACI | CCGC | 2 cut(s) | 218, 233 |
| BspANI | GGCC | 1 cut(s) | 248 |
| BspCNI | CTCAG | 3 cut(s) | 244, 346, 448 |
| BspLI | GGNNCC | 1 cut(s) | 249 |
| BspMAI | CTGCAG | 1 cut(s) | 370 |
| BspMI | ACCTGC | 1 cut(s) | 337 |
| BspQI | GCTCTTC | 1 cut(s) | 66 |
| BssECI | CCNNGG | 1 cut(s) | 388 |
| Bst2UI | CCWGG | 1 cut(s) | 183 |
| Bst6I | CTCTTC | 3 cut(s) | 24, 66, 129 |
| BstC8I | GCNNGC | 2 cut(s) | 275, 346 |
| BstDEI | CTNAG | 5 cut(s) | 77, 252, 354, 456, 504 |
| BstF5I | GGATG | 2 cut(s) | 151, 205 |
| BstMCI | CGRYCG | 1 cut(s) | 388 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 425 |
| BstNI | CCWGG | 1 cut(s) | 183 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 397 |
| BstSCI | CCNGG | 1 cut(s) | 181 |
| BstSFI | CTRYAG | 1 cut(s) | 366 |
| BstV1I | GCAGC | 3 cut(s) | 380, 415, 437 |
| Bsu36I | CCTNAGG | 2 cut(s) | 252, 456 |
| BsuRI | GGCC | 1 cut(s) | 248 |
| BtrI | CACGTC | 1 cut(s) | 179 |
| BtsCI | GGATG | 2 cut(s) | 151, 205 |
| BveI | ACCTGC | 1 cut(s) | 337 |
| Cac8I | GCNNGC | 2 cut(s) | 275, 346 |
| Cfr13I | GGNCC | 1 cut(s) | 247 |
| CviJI | RGCY | 3 cut(s) | 76, 248, 428 |
| CviKI_1 | RGCY | 3 cut(s) | 76, 248, 428 |
| DdeI | CTNAG | 5 cut(s) | 77, 252, 354, 456, 504 |
| Eam1104I | CTCTTC | 3 cut(s) | 24, 66, 129 |
| EarI | CTCTTC | 3 cut(s) | 24, 66, 129 |
| EciI | GGCGGA | 1 cut(s) | 222 |
| Eco81I | CCTNAGG | 2 cut(s) | 252, 456 |
| EcoO109I | RGGNCCY | 1 cut(s) | 247 |
| EcoRII | CCWGG | 1 cut(s) | 181 |
| Fnu4HI | GCNGC | 3 cut(s) | 369, 426, 429 |
| FokI | GGATG | 2 cut(s) | 158, 192 |
| Fsp4HI | GCNGC | 3 cut(s) | 369, 426, 429 |
| FspBI | CTAG | 1 cut(s) | 222 |
| GluI | GCNGC | 3 cut(s) | 369, 426, 429 |
| HaeIII | GGCC | 1 cut(s) | 248 |
| HincII | GTYRAC | 1 cut(s) | 264 |
| HindII | GTYRAC | 1 cut(s) | 264 |
| HinfI | GANTC | 4 cut(s) | 164, 197, 350, 436 |
| HphI | GGTGA | 4 cut(s) | 159, 197, 393, 435 |
| Hpy166II | GTNNAC | 1 cut(s) | 264 |
| Hpy188I | TCNGA | 1 cut(s) | 355 |
| Hpy188III | TCNNGA | 1 cut(s) | 455 |
| Hpy8I | GTNNAC | 1 cut(s) | 264 |
| HpyAV | CCTTC | 1 cut(s) | 133 |
| HpyCH4IV | ACGT | 1 cut(s) | 178 |
| HpyCH4V | TGCA | 3 cut(s) | 368, 431, 489 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 425 |
| HpyF3I | CTNAG | 5 cut(s) | 77, 252, 354, 456, 504 |
| HpySE526I | ACGT | 1 cut(s) | 178 |
| LguI | GCTCTTC | 1 cut(s) | 66 |
| LpnPI | CCDG | 5 cut(s) | 168, 195, 264, 342, 468 |
| Lsp1109I | GCAGC | 3 cut(s) | 380, 415, 437 |
| MaeI | CTAG | 1 cut(s) | 222 |
| MaeII | ACGT | 1 cut(s) | 178 |
| MaeIII | GTNAC | 2 cut(s) | 165, 399 |
| MboII | GAAGA | 6 cut(s) | 31, 41, 80, 83, 146, 491 |
| MluCI | AATT | 3 cut(s) | 41, 117, 464 |
| MlyI | GAGTC | 2 cut(s) | 173, 191 |
| MmeI | TCCRAC | 1 cut(s) | 36 |
| MnlI | CCTC | 9 cut(s) | 94, 148, 219, 240, 247, 383, 384, 451, 460 |
| MseI | TTAA | 1 cut(s) | 462 |
| MslI | CAYNNNNRTG | 1 cut(s) | 409 |
| MspA1I | CMGCKG | 1 cut(s) | 428 |
| MspR9I | CCNGG | 1 cut(s) | 183 |
| MvaI | CCWGG | 1 cut(s) | 183 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 425 |
| NlaIV | GGNNCC | 1 cut(s) | 249 |
| NmuCI | GTSAC | 2 cut(s) | 165, 399 |
| PciSI | GCTCTTC | 1 cut(s) | 66 |
| PfeI | GAWTC | 2 cut(s) | 350, 436 |
| PkrI | GCNGC | 3 cut(s) | 370, 427, 430 |
| PleI | GAGTC | 2 cut(s) | 172, 191 |
| PpsI | GAGTC | 2 cut(s) | 172, 191 |
| PshAI | GACNNNNGTC | 1 cut(s) | 397 |
| Psp6I | CCWGG | 1 cut(s) | 181 |
| PspGI | CCWGG | 1 cut(s) | 181 |
| PspN4I | GGNNCC | 1 cut(s) | 249 |
| PspPI | GGNCC | 1 cut(s) | 247 |
| PstI | CTGCAG | 1 cut(s) | 370 |
| PvuII | CAGCTG | 1 cut(s) | 428 |
| RseI | CAYNNNNRTG | 1 cut(s) | 409 |
| SapI | GCTCTTC | 1 cut(s) | 66 |
| SaqAI | TTAA | 1 cut(s) | 462 |
| SatI | GCNGC | 3 cut(s) | 369, 426, 429 |
| Sau96I | GGNCC | 1 cut(s) | 247 |
| SchI | GAGTC | 2 cut(s) | 173, 191 |
| ScrFI | CCNGG | 1 cut(s) | 183 |
| SetI | ASST | 9 cut(s) | 78, 181, 187, 304, 331, 376, 430, 452, 462 |
| SfcI | CTRYAG | 1 cut(s) | 366 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 409 |
| SmlI | CTYRAG | 1 cut(s) | 297 |
| SmoI | CTYRAG | 1 cut(s) | 297 |
| Sse9I | AATT | 3 cut(s) | 41, 117, 464 |
| SsiI | CCGC | 2 cut(s) | 218, 233 |
| SspMI | CTAG | 1 cut(s) | 222 |
| StyD4I | CCNGG | 1 cut(s) | 181 |
| TaiI | ACGT | 1 cut(s) | 181 |
| TaqII | GACCGA | 1 cut(s) | 402 |
| TasI | AATT | 3 cut(s) | 41, 117, 464 |
| TfiI | GAWTC | 2 cut(s) | 350, 436 |
| Tru1I | TTAA | 1 cut(s) | 462 |
| Tru9I | TTAA | 1 cut(s) | 462 |
| TseFI | GTSAC | 2 cut(s) | 165, 399 |
| TseI | GCWGC | 3 cut(s) | 368, 425, 428 |
| Tsp45I | GTSAC | 2 cut(s) | 165, 399 |
| TspDTI | ATGAA | 2 cut(s) | 42, 428 |
| XspI | CTAG | 1 cut(s) | 222 |