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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 266 |
| AciI | CCGC | 1 cut(s) | 134 |
| AclWI | GGATC | 2 cut(s) | 36, 49 |
| AfaI | GTAC | 1 cut(s) | 57 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 44, 72, 266 |
| AgsI | TTSAA | 2 cut(s) | 154, 235 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 146, 178, 265, 297 |
| AluBI | AGCT | 2 cut(s) | 149, 333 |
| AluI | AGCT | 2 cut(s) | 149, 333 |
| AlwI | GGATC | 2 cut(s) | 36, 49 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 266 |
| AseI | ATTAAT | 1 cut(s) | 224 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 239 |
| AsuC2I | CCSGG | 2 cut(s) | 39, 46 |
| BamHI | GGATCC | 1 cut(s) | 41 |
| BceAI | ACGGC | 1 cut(s) | 203 |
| BcnI | CCSGG | 2 cut(s) | 39, 46 |
| BfmI | CTRYAG | 2 cut(s) | 138, 174 |
| BisI | GCNGC | 1 cut(s) | 135 |
| BlsI | GCNGC | 1 cut(s) | 136 |
| Bme1390I | CCNGG | 2 cut(s) | 39, 46 |
| BmiI | GGNNCC | 1 cut(s) | 43 |
| BmrFI | CCNGG | 2 cut(s) | 39, 46 |
| BpmI | CTGGAG | 1 cut(s) | 288 |
| BpuMI | CCSGG | 2 cut(s) | 39, 46 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 44, 72, 266 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 44, 72, 266 |
| BsiSI | CCGG | 2 cut(s) | 38, 45 |
| BslFI | GGGAC | 2 cut(s) | 218, 299 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 44, 72, 266 |
| BsmFI | GGGAC | 2 cut(s) | 218, 299 |
| Bsp143I | GATC | 1 cut(s) | 41 |
| BspACI | CCGC | 1 cut(s) | 134 |
| BspLI | GGNNCC | 1 cut(s) | 43 |
| BspPI | GGATC | 2 cut(s) | 36, 49 |
| BssMI | GATC | 1 cut(s) | 41 |
| Bst6I | CTCTTC | 1 cut(s) | 300 |
| BstKTI | GATC | 1 cut(s) | 44 |
| BstMBI | GATC | 1 cut(s) | 41 |
| BstSCI | CCNGG | 2 cut(s) | 37, 44 |
| BstSFI | CTRYAG | 2 cut(s) | 138, 174 |
| BstX2I | RGATCY | 1 cut(s) | 41 |
| BstYI | RGATCY | 1 cut(s) | 41 |
| CaiI | CAGNNNCTG | 1 cut(s) | 266 |
| Csp6I | GTAC | 1 cut(s) | 56 |
| CviJI | RGCY | 3 cut(s) | 137, 149, 333 |
| CviKI_1 | RGCY | 3 cut(s) | 137, 149, 333 |
| CviQI | GTAC | 1 cut(s) | 56 |
| DpnI | GATC | 1 cut(s) | 43 |
| DpnII | GATC | 1 cut(s) | 41 |
| Eam1104I | CTCTTC | 1 cut(s) | 300 |
| EarI | CTCTTC | 1 cut(s) | 300 |
| FaiI | YATR | 7 cut(s) | 17, 23, 79, 87, 291, 293, 330 |
| FaqI | GGGAC | 2 cut(s) | 218, 299 |
| Fnu4HI | GCNGC | 1 cut(s) | 135 |
| Fsp4HI | GCNGC | 1 cut(s) | 135 |
| GluI | GCNGC | 1 cut(s) | 135 |
| GsuI | CTGGAG | 1 cut(s) | 288 |
| HapII | CCGG | 2 cut(s) | 38, 45 |
| HincII | GTYRAC | 1 cut(s) | 6 |
| HindII | GTYRAC | 1 cut(s) | 6 |
| HindIII | AAGCTT | 1 cut(s) | 147 |
| HinfI | GANTC | 3 cut(s) | 123, 263, 297 |
| HpaII | CCGG | 2 cut(s) | 38, 45 |
| Hpy166II | GTNNAC | 1 cut(s) | 6 |
| Hpy188III | TCNNGA | 2 cut(s) | 94, 267 |
| Hpy8I | GTNNAC | 1 cut(s) | 6 |
| HpyAV | CCTTC | 2 cut(s) | 65, 151 |
| Kzo9I | GATC | 1 cut(s) | 41 |
| LpnPI | CCDG | 7 cut(s) | 51, 58, 168, 252, 266, 273, 305 |
| MaeIII | GTNAC | 1 cut(s) | 94 |
| MalI | GATC | 1 cut(s) | 43 |
| MboI | GATC | 1 cut(s) | 41 |
| MboII | GAAGA | 3 cut(s) | 166, 200, 317 |
| MflI | RGATCY | 1 cut(s) | 41 |
| MlyI | GAGTC | 2 cut(s) | 257, 306 |
| MnlI | CCTC | 3 cut(s) | 136, 263, 301 |
| MroXI | GAANNNNTTC | 1 cut(s) | 239 |
| MseI | TTAA | 1 cut(s) | 224 |
| MspI | CCGG | 2 cut(s) | 38, 45 |
| MspR9I | CCNGG | 2 cut(s) | 39, 46 |
| NciI | CCSGG | 2 cut(s) | 39, 46 |
| NdeII | GATC | 1 cut(s) | 41 |
| NlaIV | GGNNCC | 1 cut(s) | 43 |
| NmuCI | GTSAC | 1 cut(s) | 94 |
| PdmI | GAANNNNTTC | 1 cut(s) | 239 |
| PfeI | GAWTC | 1 cut(s) | 123 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 266 |
| PkrI | GCNGC | 1 cut(s) | 136 |
| PleI | GAGTC | 2 cut(s) | 257, 305 |
| PpsI | GAGTC | 2 cut(s) | 257, 305 |
| PshBI | ATTAAT | 1 cut(s) | 224 |
| PspN4I | GGNNCC | 1 cut(s) | 43 |
| PstNI | CAGNNNCTG | 1 cut(s) | 266 |
| PsuI | RGATCY | 1 cut(s) | 41 |
| RsaI | GTAC | 1 cut(s) | 57 |
| RsaNI | GTAC | 1 cut(s) | 56 |
| SaqAI | TTAA | 1 cut(s) | 224 |
| SatI | GCNGC | 1 cut(s) | 135 |
| Sau3AI | GATC | 1 cut(s) | 41 |
| SchI | GAGTC | 2 cut(s) | 257, 306 |
| ScrFI | CCNGG | 2 cut(s) | 39, 46 |
| SetI | ASST | 4 cut(s) | 151, 240, 312, 335 |
| SfcI | CTRYAG | 2 cut(s) | 138, 174 |
| SsiI | CCGC | 1 cut(s) | 134 |
| StyD4I | CCNGG | 2 cut(s) | 37, 44 |
| TauI | GCSGC | 1 cut(s) | 137 |
| TfiI | GAWTC | 1 cut(s) | 123 |
| Tru1I | TTAA | 1 cut(s) | 224 |
| Tru9I | TTAA | 1 cut(s) | 224 |
| TseFI | GTSAC | 1 cut(s) | 94 |
| Tsp45I | GTSAC | 1 cut(s) | 94 |
| TspDTI | ATGAA | 1 cut(s) | 232 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 266 |
| VspI | ATTAAT | 1 cut(s) | 224 |
| XmnI | GAANNNNTTC | 1 cut(s) | 239 |