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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 436 |
| Acc36I | ACCTGC | 2 cut(s) | 232, 436 |
| AccII | CGCG | 1 cut(s) | 437 |
| AciI | CCGC | 2 cut(s) | 431, 435 |
| AcoI | YGGCCR | 1 cut(s) | 432 |
| AfaI | GTAC | 1 cut(s) | 267 |
| AgsI | TTSAA | 5 cut(s) | 206, 305, 527, 545, 797 |
| AluBI | AGCT | 5 cut(s) | 55, 76, 323, 587, 805 |
| AluI | AGCT | 5 cut(s) | 55, 76, 323, 587, 805 |
| AoxI | GGCC | 2 cut(s) | 432, 643 |
| ApeKI | GCWGC | 3 cut(s) | 320, 323, 802 |
| AseI | ATTAAT | 1 cut(s) | 405 |
| AspLEI | GCGC | 1 cut(s) | 245 |
| AsuHPI | GGTGA | 4 cut(s) | 288, 330, 586, 736 |
| AsuII | TTCGAA | 1 cut(s) | 471 |
| AxyI | CCTNAGG | 2 cut(s) | 147, 351 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 482, 515 |
| BbvI | GCAGC | 3 cut(s) | 310, 332, 814 |
| BfaI | CTAG | 3 cut(s) | 117, 518, 806 |
| BfmI | CTRYAG | 1 cut(s) | 261 |
| BfuAI | ACCTGC | 2 cut(s) | 232, 436 |
| BisI | GCNGC | 5 cut(s) | 321, 324, 432, 435, 803 |
| BlsI | GCNGC | 5 cut(s) | 322, 325, 433, 436, 804 |
| BmsI | GCATC | 2 cut(s) | 254, 367 |
| BpmI | CTGGAG | 1 cut(s) | 77 |
| Bpu14I | TTCGAA | 1 cut(s) | 471 |
| BpuEI | CTTGAG | 1 cut(s) | 177 |
| BsaJI | CCNNGG | 1 cut(s) | 283 |
| Bse1I | ACTGG | 1 cut(s) | 548 |
| Bse21I | CCTNAGG | 2 cut(s) | 147, 351 |
| BseDI | CCNNGG | 1 cut(s) | 283 |
| BseGI | GGATG | 2 cut(s) | 672, 850 |
| BseMII | CTCAG | 4 cut(s) | 240, 342, 579, 645 |
| BseNI | ACTGG | 1 cut(s) | 548 |
| BseRI | GAGGAG | 2 cut(s) | 93, 824 |
| BseX3I | CGGCCG | 1 cut(s) | 432 |
| BseXI | GCAGC | 3 cut(s) | 310, 332, 814 |
| BsgI | GTGCAG | 1 cut(s) | 309 |
| Bsh1236I | CGCG | 1 cut(s) | 437 |
| Bsh1285I | CGRYCG | 2 cut(s) | 283, 435 |
| BshFI | GGCC | 2 cut(s) | 434, 645 |
| BsiEI | CGRYCG | 2 cut(s) | 283, 435 |
| BslFI | GGGAC | 1 cut(s) | 731 |
| BsmFI | GGGAC | 1 cut(s) | 731 |
| BsnI | GGCC | 2 cut(s) | 434, 645 |
| Bsp119I | TTCGAA | 1 cut(s) | 471 |
| Bsp143I | GATC | 2 cut(s) | 443, 504 |
| BspACI | CCGC | 2 cut(s) | 431, 435 |
| BspANI | GGCC | 2 cut(s) | 434, 645 |
| BspCNI | CTCAG | 4 cut(s) | 241, 343, 580, 644 |
| BspFNI | CGCG | 1 cut(s) | 437 |
| BspMAI | CTGCAG | 1 cut(s) | 265 |
| BspMI | ACCTGC | 2 cut(s) | 232, 436 |
| BspQI | GCTCTTC | 1 cut(s) | 66 |
| BspT104I | TTCGAA | 1 cut(s) | 471 |
| BsrI | ACTGG | 1 cut(s) | 548 |
| BssECI | CCNNGG | 1 cut(s) | 283 |
| BssMI | GATC | 2 cut(s) | 443, 504 |
| Bst6I | CTCTTC | 3 cut(s) | 24, 66, 665 |
| BstBI | TTCGAA | 1 cut(s) | 471 |
| BstC8I | GCNNGC | 2 cut(s) | 241, 382 |
| BstDEI | CTNAG | 6 cut(s) | 77, 147, 249, 351, 588, 631 |
| BstF5I | GGATG | 2 cut(s) | 672, 850 |
| BstFNI | CGCG | 1 cut(s) | 437 |
| BstHHI | GCGC | 1 cut(s) | 245 |
| BstKTI | GATC | 2 cut(s) | 446, 507 |
| BstMBI | GATC | 2 cut(s) | 443, 504 |
| BstMCI | CGRYCG | 2 cut(s) | 283, 435 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 320 |
| BstSFI | CTRYAG | 1 cut(s) | 261 |
| BstUI | CGCG | 1 cut(s) | 437 |
| BstV1I | GCAGC | 3 cut(s) | 310, 332, 814 |
| BstZI | CGGCCG | 1 cut(s) | 432 |
| Bsu36I | CCTNAGG | 2 cut(s) | 147, 351 |
| BsuRI | GGCC | 2 cut(s) | 434, 645 |
| BtsCI | GGATG | 2 cut(s) | 672, 850 |
| BtsI | GCAGTG | 2 cut(s) | 110, 774 |
| BtsIMutI | CAGTG | 2 cut(s) | 110, 774 |
| BveI | ACCTGC | 2 cut(s) | 232, 436 |
| Cac8I | GCNNGC | 2 cut(s) | 241, 382 |
| CciNI | GCGGCCGC | 1 cut(s) | 432 |
| CfoI | GCGC | 1 cut(s) | 245 |
| Csp6I | GTAC | 1 cut(s) | 266 |
| CviAII | CATG | 3 cut(s) | 598, 824, 839 |
| CviJI | RGCY | 8 cut(s) | 55, 76, 157, 323, 434, 587, 645, 805 |
| CviKI_1 | RGCY | 8 cut(s) | 55, 76, 157, 323, 434, 587, 645, 805 |
| CviQI | GTAC | 1 cut(s) | 266 |
| DdeI | CTNAG | 6 cut(s) | 77, 147, 249, 351, 588, 631 |
| DpnI | GATC | 2 cut(s) | 445, 506 |
| DpnII | GATC | 2 cut(s) | 443, 504 |
| EaeI | YGGCCR | 1 cut(s) | 432 |
| EagI | CGGCCG | 1 cut(s) | 432 |
| Eam1104I | CTCTTC | 3 cut(s) | 24, 66, 665 |
| EarI | CTCTTC | 3 cut(s) | 24, 66, 665 |
| EclXI | CGGCCG | 1 cut(s) | 432 |
| Eco52I | CGGCCG | 1 cut(s) | 432 |
| Eco81I | CCTNAGG | 2 cut(s) | 147, 351 |
| FaeI | CATG | 3 cut(s) | 601, 827, 842 |
| FaiI | YATR | 8 cut(s) | 512, 569, 599, 639, 657, 764, 825, 840 |
| FaqI | GGGAC | 1 cut(s) | 731 |
| FatI | CATG | 3 cut(s) | 597, 823, 838 |
| Fnu4HI | GCNGC | 5 cut(s) | 321, 324, 432, 435, 803 |
| FokI | GGATG | 2 cut(s) | 679, 857 |
| Fsp4HI | GCNGC | 5 cut(s) | 321, 324, 432, 435, 803 |
| FspBI | CTAG | 3 cut(s) | 117, 518, 806 |
| GlaI | GCGC | 1 cut(s) | 244 |
| GluI | GCNGC | 5 cut(s) | 321, 324, 432, 435, 803 |
| GsuI | CTGGAG | 1 cut(s) | 77 |
| HaeIII | GGCC | 2 cut(s) | 434, 645 |
| HhaI | GCGC | 1 cut(s) | 245 |
| Hin1II | CATG | 3 cut(s) | 601, 827, 842 |
| Hin6I | GCGC | 1 cut(s) | 243 |
| HinP1I | GCGC | 1 cut(s) | 243 |
| HinfI | GANTC | 4 cut(s) | 331, 629, 759, 835 |
| HphI | GGTGA | 4 cut(s) | 288, 330, 586, 736 |
| Hpy166II | GTNNAC | 1 cut(s) | 594 |
| Hpy188I | TCNGA | 3 cut(s) | 250, 504, 634 |
| Hpy188III | TCNNGA | 1 cut(s) | 350 |
| Hpy8I | GTNNAC | 1 cut(s) | 594 |
| HpyCH4V | TGCA | 4 cut(s) | 263, 326, 384, 688 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 320 |
| HpyF3I | CTNAG | 6 cut(s) | 77, 147, 249, 351, 588, 631 |
| Hsp92II | CATG | 3 cut(s) | 601, 827, 842 |
| HspAI | GCGC | 1 cut(s) | 243 |
| Kzo9I | GATC | 2 cut(s) | 443, 504 |
| LguI | GCTCTTC | 1 cut(s) | 66 |
| Lsp1109I | GCAGC | 3 cut(s) | 310, 332, 814 |
| LweI | GCATC | 2 cut(s) | 254, 367 |
| MaeI | CTAG | 3 cut(s) | 117, 518, 806 |
| MaeIII | GTNAC | 1 cut(s) | 438 |
| MalI | GATC | 2 cut(s) | 445, 506 |
| MboI | GATC | 2 cut(s) | 443, 504 |
| MboII | GAAGA | 9 cut(s) | 41, 46, 80, 83, 386, 485, 682, 790, 866 |
| MfeI | CAATTG | 1 cut(s) | 792 |
| MluCI | AATT | 6 cut(s) | 41, 96, 359, 553, 603, 792 |
| MlyI | GAGTC | 1 cut(s) | 623 |
| MseI | TTAA | 3 cut(s) | 357, 405, 417 |
| MslI | CAYNNNNRTG | 2 cut(s) | 767, 843 |
| MspA1I | CMGCKG | 1 cut(s) | 323 |
| MunI | CAATTG | 1 cut(s) | 792 |
| MvnI | CGCG | 1 cut(s) | 437 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 320 |
| NdeII | GATC | 2 cut(s) | 443, 504 |
| NlaIII | CATG | 3 cut(s) | 601, 827, 842 |
| NotI | GCGGCCGC | 1 cut(s) | 432 |
| NspV | TTCGAA | 1 cut(s) | 471 |
| PaqCI | CACCTGC | 1 cut(s) | 436 |
| PciSI | GCTCTTC | 1 cut(s) | 66 |
| PfeI | GAWTC | 3 cut(s) | 331, 759, 835 |
| PkrI | GCNGC | 5 cut(s) | 322, 325, 433, 436, 804 |
| PleI | GAGTC | 1 cut(s) | 623 |
| PpsI | GAGTC | 1 cut(s) | 623 |
| PshBI | ATTAAT | 1 cut(s) | 405 |
| PstI | CTGCAG | 1 cut(s) | 265 |
| PvuII | CAGCTG | 1 cut(s) | 323 |
| RsaI | GTAC | 1 cut(s) | 267 |
| RsaNI | GTAC | 1 cut(s) | 266 |
| RseI | CAYNNNNRTG | 2 cut(s) | 767, 843 |
| SapI | GCTCTTC | 1 cut(s) | 66 |
| SaqAI | TTAA | 3 cut(s) | 357, 405, 417 |
| SatI | GCNGC | 5 cut(s) | 321, 324, 432, 435, 803 |
| Sau3AI | GATC | 2 cut(s) | 443, 504 |
| SchI | GAGTC | 1 cut(s) | 623 |
| SfaNI | GCATC | 2 cut(s) | 254, 367 |
| SfcI | CTRYAG | 1 cut(s) | 261 |
| SfuI | TTCGAA | 1 cut(s) | 471 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 767, 843 |
| SmlI | CTYRAG | 1 cut(s) | 192 |
| SmoI | CTYRAG | 1 cut(s) | 192 |
| Sse9I | AATT | 6 cut(s) | 41, 96, 359, 553, 603, 792 |
| SsiI | CCGC | 2 cut(s) | 431, 435 |
| SspMI | CTAG | 3 cut(s) | 117, 518, 806 |
| TaqI | TCGA | 1 cut(s) | 471 |
| TaqII | GACCGA | 1 cut(s) | 297 |
| TasI | AATT | 6 cut(s) | 41, 96, 359, 553, 603, 792 |
| TauI | GCSGC | 2 cut(s) | 434, 437 |
| TfiI | GAWTC | 3 cut(s) | 331, 759, 835 |
| Tru1I | TTAA | 3 cut(s) | 357, 405, 417 |
| Tru9I | TTAA | 3 cut(s) | 357, 405, 417 |
| TscAI | CASTG | 2 cut(s) | 117, 774 |
| TseI | GCWGC | 3 cut(s) | 320, 323, 802 |
| TspDTI | ATGAA | 5 cut(s) | 42, 54, 323, 683, 827 |
| TspRI | CASTG | 2 cut(s) | 117, 774 |
| VspI | ATTAAT | 1 cut(s) | 405 |
| XspI | CTAG | 3 cut(s) | 117, 518, 806 |