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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 144 |
| AcoI | YGGCCR | 1 cut(s) | 9 |
| AcsI | RAATTY | 1 cut(s) | 152 |
| AflIII | ACRYGT | 2 cut(s) | 54, 98 |
| AgsI | TTSAA | 3 cut(s) | 40, 122, 236 |
| AjuI | GAANNNNNNNTTGG | 1 cut(s) | 37 |
| AluBI | AGCT | 2 cut(s) | 127, 194 |
| AluI | AGCT | 2 cut(s) | 127, 194 |
| AlwI | GGATC | 1 cut(s) | 144 |
| AoxI | GGCC | 2 cut(s) | 9, 33 |
| ApoI | RAATTY | 1 cut(s) | 152 |
| BalI | TGGCCA | 1 cut(s) | 11 |
| BclI | TGATCA | 1 cut(s) | 223 |
| BfaI | CTAG | 1 cut(s) | 254 |
| BpmI | CTGGAG | 1 cut(s) | 180 |
| BpuEI | CTTGAG | 1 cut(s) | 113 |
| BsaBI | GATNNNNATC | 1 cut(s) | 222 |
| Bse1I | ACTGG | 1 cut(s) | 36 |
| Bse8I | GATNNNNATC | 1 cut(s) | 222 |
| BseJI | GATNNNNATC | 1 cut(s) | 222 |
| BseNI | ACTGG | 1 cut(s) | 36 |
| BseRI | GAGGAG | 1 cut(s) | 179 |
| BshFI | GGCC | 2 cut(s) | 11, 35 |
| BsnI | GGCC | 2 cut(s) | 11, 35 |
| Bsp143I | GATC | 3 cut(s) | 136, 173, 223 |
| BspANI | GGCC | 2 cut(s) | 11, 35 |
| BspPI | GGATC | 1 cut(s) | 144 |
| BsrI | ACTGG | 1 cut(s) | 36 |
| BssMI | GATC | 3 cut(s) | 136, 173, 223 |
| Bst4CI | ACNGT | 1 cut(s) | 46 |
| Bst6I | CTCTTC | 1 cut(s) | 156 |
| BstKTI | GATC | 3 cut(s) | 139, 176, 226 |
| BstMBI | GATC | 3 cut(s) | 136, 173, 223 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 191 |
| BstNSI | RCATGY | 3 cut(s) | 58, 82, 102 |
| BsuRI | GGCC | 2 cut(s) | 11, 35 |
| BtsIMutI | CAGTG | 2 cut(s) | 29, 83 |
| CviAII | CATG | 4 cut(s) | 25, 55, 79, 99 |
| CviJI | RGCY | 5 cut(s) | 11, 35, 127, 194, 269 |
| CviKI_1 | RGCY | 5 cut(s) | 11, 35, 127, 194, 269 |
| DpnI | GATC | 3 cut(s) | 138, 175, 225 |
| DpnII | GATC | 3 cut(s) | 136, 173, 223 |
| EaeI | YGGCCR | 1 cut(s) | 9 |
| Eam1104I | CTCTTC | 1 cut(s) | 156 |
| EarI | CTCTTC | 1 cut(s) | 156 |
| EcoRI | GAATTC | 1 cut(s) | 152 |
| FaeI | CATG | 4 cut(s) | 28, 58, 82, 102 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 37 |
| FatI | CATG | 4 cut(s) | 24, 54, 78, 98 |
| FauNDI | CATATG | 1 cut(s) | 91 |
| FbaI | TGATCA | 1 cut(s) | 223 |
| FspBI | CTAG | 1 cut(s) | 254 |
| GsuI | CTGGAG | 1 cut(s) | 180 |
| HaeIII | GGCC | 2 cut(s) | 11, 35 |
| Hin1II | CATG | 4 cut(s) | 28, 58, 82, 102 |
| HinfI | GANTC | 1 cut(s) | 146 |
| Hpy166II | GTNNAC | 1 cut(s) | 52 |
| Hpy188I | TCNGA | 2 cut(s) | 151, 178 |
| Hpy188III | TCNNGA | 2 cut(s) | 140, 197 |
| Hpy8I | GTNNAC | 1 cut(s) | 52 |
| HpyAV | CCTTC | 3 cut(s) | 46, 194, 206 |
| HpyCH4III | ACNGT | 1 cut(s) | 46 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 191 |
| Hsp92II | CATG | 4 cut(s) | 28, 58, 82, 102 |
| Ksp22I | TGATCA | 1 cut(s) | 223 |
| Kzo9I | GATC | 3 cut(s) | 136, 173, 223 |
| LmnI | GCTCC | 1 cut(s) | 199 |
| LpnPI | CCDG | 2 cut(s) | 17, 210 |
| MaeI | CTAG | 1 cut(s) | 254 |
| MaeIII | GTNAC | 4 cut(s) | 15, 27, 69, 81 |
| MalI | GATC | 3 cut(s) | 138, 175, 225 |
| MboI | GATC | 3 cut(s) | 136, 173, 223 |
| MboII | GAAGA | 4 cut(s) | 173, 218, 221, 254 |
| MlsI | TGGCCA | 1 cut(s) | 11 |
| MluCI | AATT | 2 cut(s) | 152, 229 |
| MluNI | TGGCCA | 1 cut(s) | 11 |
| MnlI | CCTC | 5 cut(s) | 127, 136, 151, 157, 256 |
| Mox20I | TGGCCA | 1 cut(s) | 11 |
| MscI | TGGCCA | 1 cut(s) | 11 |
| Msp20I | TGGCCA | 1 cut(s) | 11 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 191 |
| NdeI | CATATG | 1 cut(s) | 91 |
| NdeII | GATC | 3 cut(s) | 136, 173, 223 |
| NlaIII | CATG | 4 cut(s) | 28, 58, 82, 102 |
| NmuCI | GTSAC | 4 cut(s) | 15, 27, 69, 81 |
| NspI | RCATGY | 3 cut(s) | 58, 82, 102 |
| PciI | ACATGT | 2 cut(s) | 54, 98 |
| PfeI | GAWTC | 1 cut(s) | 146 |
| PscI | ACATGT | 2 cut(s) | 54, 98 |
| Sau3AI | GATC | 3 cut(s) | 136, 173, 223 |
| SetI | ASST | 4 cut(s) | 129, 196, 255, 267 |
| SmlI | CTYRAG | 1 cut(s) | 128 |
| SmoI | CTYRAG | 1 cut(s) | 128 |
| Sse9I | AATT | 2 cut(s) | 152, 229 |
| SspI | AATATT | 1 cut(s) | 117 |
| SspMI | CTAG | 1 cut(s) | 254 |
| TaaI | ACNGT | 1 cut(s) | 46 |
| TaqI | TCGA | 2 cut(s) | 139, 156 |
| TasI | AATT | 2 cut(s) | 152, 229 |
| TfiI | GAWTC | 1 cut(s) | 146 |
| TscAI | CASTG | 2 cut(s) | 36, 90 |
| TseFI | GTSAC | 4 cut(s) | 15, 27, 69, 81 |
| Tsp45I | GTSAC | 4 cut(s) | 15, 27, 69, 81 |
| TspDTI | ATGAA | 3 cut(s) | 13, 98, 195 |
| TspRI | CASTG | 2 cut(s) | 36, 90 |
| XapI | RAATTY | 1 cut(s) | 152 |
| XceI | RCATGY | 3 cut(s) | 58, 82, 102 |
| XspI | CTAG | 1 cut(s) | 254 |