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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 52 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 266, 488 |
| AciI | CCGC | 1 cut(s) | 356 |
| AclWI | GGATC | 2 cut(s) | 271, 353 |
| AcsI | RAATTY | 1 cut(s) | 215 |
| AcuI | CTGAAG | 1 cut(s) | 69 |
| AfaI | GTAC | 1 cut(s) | 279 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 266, 294, 404, 488 |
| AgsI | TTSAA | 3 cut(s) | 117, 376, 457 |
| AjnI | CCWGG | 1 cut(s) | 259 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 368, 400, 487, 519 |
| AluBI | AGCT | 3 cut(s) | 64, 371, 555 |
| AluI | AGCT | 3 cut(s) | 64, 371, 555 |
| AlwI | GGATC | 2 cut(s) | 271, 353 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 266 |
| ApeKI | GCWGC | 3 cut(s) | 64, 67, 359 |
| ApoI | RAATTY | 1 cut(s) | 215 |
| AseI | ATTAAT | 1 cut(s) | 446 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 461 |
| BbvI | GCAGC | 3 cut(s) | 51, 54, 346 |
| BceAI | ACGGC | 1 cut(s) | 425 |
| BciT130I | CCWGG | 1 cut(s) | 261 |
| BfaI | CTAG | 1 cut(s) | 556 |
| BfmI | CTRYAG | 2 cut(s) | 68, 360 |
| BisI | GCNGC | 4 cut(s) | 65, 68, 357, 360 |
| BlsI | GCNGC | 4 cut(s) | 66, 69, 358, 361 |
| Bme1390I | CCNGG | 1 cut(s) | 261 |
| BmrFI | CCNGG | 1 cut(s) | 261 |
| BmsI | GCATC | 2 cut(s) | 88, 121 |
| BpmI | CTGGAG | 2 cut(s) | 381, 510 |
| BsaJI | CCNNGG | 1 cut(s) | 106 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 266, 294, 404, 488 |
| BseBI | CCWGG | 1 cut(s) | 261 |
| BseDI | CCNNGG | 1 cut(s) | 106 |
| BseGI | GGATG | 1 cut(s) | 136 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 266, 294, 404, 488 |
| BseMII | CTCAG | 1 cut(s) | 300 |
| BseXI | GCAGC | 3 cut(s) | 51, 54, 346 |
| BslFI | GGGAC | 2 cut(s) | 440, 521 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 266, 294, 404, 488 |
| BsmFI | GGGAC | 2 cut(s) | 440, 521 |
| BsmI | GAATGC | 1 cut(s) | 101 |
| Bsp143I | GATC | 2 cut(s) | 263, 345 |
| Bsp19I | CCATGG | 1 cut(s) | 106 |
| BspACI | CCGC | 1 cut(s) | 356 |
| BspCNI | CTCAG | 1 cut(s) | 301 |
| BspMAI | CTGCAG | 2 cut(s) | 72, 364 |
| BspPI | GGATC | 2 cut(s) | 271, 353 |
| BssECI | CCNNGG | 1 cut(s) | 106 |
| BssMI | GATC | 2 cut(s) | 263, 345 |
| BssT1I | CCWWGG | 1 cut(s) | 106 |
| Bst2UI | CCWGG | 1 cut(s) | 261 |
| Bst4CI | ACNGT | 1 cut(s) | 182 |
| Bst6I | CTCTTC | 2 cut(s) | 335, 522 |
| BstDEI | CTNAG | 1 cut(s) | 309 |
| BstDSI | CCRYGG | 1 cut(s) | 106 |
| BstF5I | GGATG | 1 cut(s) | 136 |
| BstKTI | GATC | 2 cut(s) | 266, 348 |
| BstMBI | GATC | 2 cut(s) | 263, 345 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 368 |
| BstNI | CCWGG | 1 cut(s) | 261 |
| BstSCI | CCNGG | 1 cut(s) | 259 |
| BstSFI | CTRYAG | 2 cut(s) | 68, 360 |
| BstV1I | GCAGC | 3 cut(s) | 51, 54, 346 |
| BstX2I | RGATCY | 1 cut(s) | 263 |
| BstYI | RGATCY | 1 cut(s) | 263 |
| BtgI | CCRYGG | 1 cut(s) | 106 |
| BtsCI | GGATG | 1 cut(s) | 136 |
| BtsIMutI | CAGTG | 1 cut(s) | 187 |
| CaiI | CAGNNNCTG | 1 cut(s) | 266 |
| Csp6I | GTAC | 1 cut(s) | 278 |
| CviAII | CATG | 1 cut(s) | 107 |
| CviJI | RGCY | 4 cut(s) | 64, 359, 371, 555 |
| CviKI_1 | RGCY | 4 cut(s) | 64, 359, 371, 555 |
| CviQI | GTAC | 1 cut(s) | 278 |
| DdeI | CTNAG | 1 cut(s) | 309 |
| DpnI | GATC | 2 cut(s) | 265, 347 |
| DpnII | GATC | 2 cut(s) | 263, 345 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 52 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 52 |
| Eam1104I | CTCTTC | 2 cut(s) | 335, 522 |
| EarI | CTCTTC | 2 cut(s) | 335, 522 |
| Eco130I | CCWWGG | 1 cut(s) | 106 |
| Eco57I | CTGAAG | 1 cut(s) | 69 |
| EcoRII | CCWGG | 1 cut(s) | 259 |
| EcoT14I | CCWWGG | 1 cut(s) | 106 |
| EcoT22I | ATGCAT | 1 cut(s) | 103 |
| ErhI | CCWWGG | 1 cut(s) | 106 |
| FaeI | CATG | 1 cut(s) | 110 |
| FaiI | YATR | 8 cut(s) | 108, 175, 239, 245, 301, 513, 515, 552 |
| FaqI | GGGAC | 2 cut(s) | 440, 521 |
| FatI | CATG | 1 cut(s) | 106 |
| Fnu4HI | GCNGC | 4 cut(s) | 65, 68, 357, 360 |
| FokI | GGATG | 1 cut(s) | 143 |
| Fsp4HI | GCNGC | 4 cut(s) | 65, 68, 357, 360 |
| FspBI | CTAG | 1 cut(s) | 556 |
| GluI | GCNGC | 4 cut(s) | 65, 68, 357, 360 |
| GsuI | CTGGAG | 2 cut(s) | 381, 510 |
| Hin1II | CATG | 1 cut(s) | 110 |
| HincII | GTYRAC | 1 cut(s) | 228 |
| HindII | GTYRAC | 1 cut(s) | 228 |
| HindIII | AAGCTT | 1 cut(s) | 369 |
| HinfI | GANTC | 1 cut(s) | 519 |
| Hpy166II | GTNNAC | 1 cut(s) | 228 |
| Hpy188I | TCNGA | 4 cut(s) | 15, 145, 221, 310 |
| Hpy188III | TCNNGA | 3 cut(s) | 316, 398, 489 |
| Hpy8I | GTNNAC | 1 cut(s) | 228 |
| HpyAV | CCTTC | 3 cut(s) | 44, 287, 370 |
| HpyCH4III | ACNGT | 1 cut(s) | 182 |
| HpyCH4V | TGCA | 6 cut(s) | 26, 70, 86, 101, 134, 362 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 368 |
| HpyF3I | CTNAG | 1 cut(s) | 309 |
| Hsp92II | CATG | 1 cut(s) | 110 |
| Kzo9I | GATC | 2 cut(s) | 263, 345 |
| LpnPI | CCDG | 9 cut(s) | 56, 196, 246, 252, 273, 390, 411, 474, 527 |
| Lsp1109I | GCAGC | 3 cut(s) | 51, 54, 346 |
| LweI | GCATC | 2 cut(s) | 88, 121 |
| MaeI | CTAG | 1 cut(s) | 556 |
| MaeIII | GTNAC | 2 cut(s) | 182, 316 |
| MalI | GATC | 2 cut(s) | 265, 347 |
| MboI | GATC | 2 cut(s) | 263, 345 |
| MboII | GAAGA | 4 cut(s) | 352, 422, 539, 558 |
| MfeI | CAATTG | 1 cut(s) | 21 |
| MflI | RGATCY | 1 cut(s) | 263 |
| MluCI | AATT | 4 cut(s) | 21, 117, 215, 470 |
| MlyI | GAGTC | 1 cut(s) | 528 |
| MnlI | CCTC | 6 cut(s) | 104, 336, 358, 373, 485, 523 |
| Mph1103I | ATGCAT | 1 cut(s) | 103 |
| MroXI | GAANNNNTTC | 1 cut(s) | 461 |
| MseI | TTAA | 3 cut(s) | 120, 152, 446 |
| MspR9I | CCNGG | 1 cut(s) | 261 |
| MunI | CAATTG | 1 cut(s) | 21 |
| Mva1269I | GAATGC | 1 cut(s) | 101 |
| MvaI | CCWGG | 1 cut(s) | 261 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 368 |
| NcoI | CCATGG | 1 cut(s) | 106 |
| NdeII | GATC | 2 cut(s) | 263, 345 |
| NlaIII | CATG | 1 cut(s) | 110 |
| NmuCI | GTSAC | 2 cut(s) | 182, 316 |
| NsiI | ATGCAT | 1 cut(s) | 103 |
| PctI | GAATGC | 1 cut(s) | 101 |
| PdmI | GAANNNNTTC | 1 cut(s) | 461 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 266, 488 |
| PkrI | GCNGC | 4 cut(s) | 66, 69, 358, 361 |
| PleI | GAGTC | 1 cut(s) | 527 |
| PpsI | GAGTC | 1 cut(s) | 527 |
| PshBI | ATTAAT | 1 cut(s) | 446 |
| Psp6I | CCWGG | 1 cut(s) | 259 |
| PspGI | CCWGG | 1 cut(s) | 259 |
| PstI | CTGCAG | 2 cut(s) | 72, 364 |
| PstNI | CAGNNNCTG | 1 cut(s) | 266 |
| PsuI | RGATCY | 1 cut(s) | 263 |
| RsaI | GTAC | 1 cut(s) | 279 |
| RsaNI | GTAC | 1 cut(s) | 278 |
| SaqAI | TTAA | 3 cut(s) | 120, 152, 446 |
| SatI | GCNGC | 4 cut(s) | 65, 68, 357, 360 |
| Sau3AI | GATC | 2 cut(s) | 263, 345 |
| SchI | GAGTC | 1 cut(s) | 528 |
| ScrFI | CCNGG | 1 cut(s) | 261 |
| SetI | ASST | 8 cut(s) | 45, 66, 115, 195, 373, 462, 534, 557 |
| SfaNI | GCATC | 2 cut(s) | 88, 121 |
| SfcI | CTRYAG | 2 cut(s) | 68, 360 |
| Sse9I | AATT | 4 cut(s) | 21, 117, 215, 470 |
| SsiI | CCGC | 1 cut(s) | 356 |
| SspMI | CTAG | 1 cut(s) | 556 |
| StyD4I | CCNGG | 1 cut(s) | 259 |
| StyI | CCWWGG | 1 cut(s) | 106 |
| TaaI | ACNGT | 1 cut(s) | 182 |
| TasI | AATT | 4 cut(s) | 21, 117, 215, 470 |
| TauI | GCSGC | 1 cut(s) | 359 |
| Tru1I | TTAA | 3 cut(s) | 120, 152, 446 |
| Tru9I | TTAA | 3 cut(s) | 120, 152, 446 |
| TscAI | CASTG | 1 cut(s) | 187 |
| TseFI | GTSAC | 2 cut(s) | 182, 316 |
| TseI | GCWGC | 3 cut(s) | 64, 67, 359 |
| Tsp45I | GTSAC | 2 cut(s) | 182, 316 |
| TspDTI | ATGAA | 1 cut(s) | 454 |
| TspRI | CASTG | 1 cut(s) | 187 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 266, 488 |
| VspI | ATTAAT | 1 cut(s) | 446 |
| XapI | RAATTY | 1 cut(s) | 215 |
| XmnI | GAANNNNTTC | 1 cut(s) | 461 |
| XspI | CTAG | 1 cut(s) | 556 |
| Zsp2I | ATGCAT | 1 cut(s) | 103 |