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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 133, 320 |
| AfaI | GTAC | 1 cut(s) | 31 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 201 |
| AflIII | ACRYGT | 1 cut(s) | 390 |
| AgeI | ACCGGT | 1 cut(s) | 380 |
| AgsI | TTSAA | 1 cut(s) | 371 |
| AluBI | AGCT | 1 cut(s) | 215 |
| AluI | AGCT | 1 cut(s) | 215 |
| Alw26I | GTCTC | 1 cut(s) | 395 |
| ApeKI | GCWGC | 3 cut(s) | 141, 215, 218 |
| AsiGI | ACCGGT | 1 cut(s) | 380 |
| AsuHPI | GGTGA | 1 cut(s) | 395 |
| BbsI | GAAGAC | 1 cut(s) | 20 |
| BbvI | GCAGC | 3 cut(s) | 128, 202, 205 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 299, 333 |
| BcoDI | GTCTC | 1 cut(s) | 395 |
| BfmI | CTRYAG | 1 cut(s) | 219 |
| BisI | GCNGC | 3 cut(s) | 142, 216, 219 |
| BlsI | GCNGC | 3 cut(s) | 143, 217, 220 |
| BmsI | GCATC | 2 cut(s) | 239, 279 |
| BpiI | GAAGAC | 1 cut(s) | 20 |
| BsaJI | CCNNGG | 1 cut(s) | 257 |
| BsaWI | WCCGGW | 1 cut(s) | 380 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 201 |
| Bse118I | RCCGGY | 1 cut(s) | 380 |
| BseDI | CCNNGG | 1 cut(s) | 257 |
| BseGI | GGATG | 1 cut(s) | 79 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 201 |
| BseXI | GCAGC | 3 cut(s) | 128, 202, 205 |
| BseYI | CCCAGC | 1 cut(s) | 94 |
| BsgI | GTGCAG | 1 cut(s) | 127 |
| Bsh1285I | CGRYCG | 1 cut(s) | 199 |
| BshTI | ACCGGT | 1 cut(s) | 380 |
| BsiEI | CGRYCG | 1 cut(s) | 199 |
| BsiSI | CCGG | 1 cut(s) | 381 |
| BslFI | GGGAC | 2 cut(s) | 77, 218 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 201 |
| BsmAI | GTCTC | 1 cut(s) | 395 |
| BsmFI | GGGAC | 2 cut(s) | 77, 218 |
| BsmI | GAATGC | 1 cut(s) | 252 |
| Bsp19I | CCATGG | 1 cut(s) | 257 |
| BspACI | CCGC | 2 cut(s) | 133, 320 |
| BspMAI | CTGCAG | 1 cut(s) | 223 |
| BsrFI | RCCGGY | 1 cut(s) | 380 |
| BssAI | RCCGGY | 1 cut(s) | 380 |
| BssECI | CCNNGG | 1 cut(s) | 257 |
| BssT1I | CCWWGG | 1 cut(s) | 257 |
| Bst6I | CTCTTC | 2 cut(s) | 75, 95 |
| BstC8I | GCNNGC | 1 cut(s) | 153 |
| BstDSI | CCRYGG | 1 cut(s) | 257 |
| BstF5I | GGATG | 1 cut(s) | 79 |
| BstMAI | GTCTC | 1 cut(s) | 395 |
| BstMCI | CGRYCG | 1 cut(s) | 199 |
| BstNSI | RCATGY | 1 cut(s) | 394 |
| BstSFI | CTRYAG | 1 cut(s) | 219 |
| BstV1I | GCAGC | 3 cut(s) | 128, 202, 205 |
| BstV2I | GAAGAC | 1 cut(s) | 20 |
| BtgI | CCRYGG | 1 cut(s) | 257 |
| BtsCI | GGATG | 1 cut(s) | 79 |
| Cac8I | GCNNGC | 1 cut(s) | 153 |
| Cfr10I | RCCGGY | 1 cut(s) | 380 |
| Csp6I | GTAC | 1 cut(s) | 30 |
| CspAI | ACCGGT | 1 cut(s) | 380 |
| CviAII | CATG | 3 cut(s) | 258, 391, 409 |
| CviJI | RGCY | 1 cut(s) | 215 |
| CviKI_1 | RGCY | 1 cut(s) | 215 |
| CviQI | GTAC | 1 cut(s) | 30 |
| Eam1104I | CTCTTC | 2 cut(s) | 75, 95 |
| EarI | CTCTTC | 2 cut(s) | 75, 95 |
| Eco130I | CCWWGG | 1 cut(s) | 257 |
| EcoT14I | CCWWGG | 1 cut(s) | 257 |
| EcoT22I | ATGCAT | 1 cut(s) | 254 |
| ErhI | CCWWGG | 1 cut(s) | 257 |
| FaeI | CATG | 3 cut(s) | 261, 394, 412 |
| FaiI | YATR | 5 cut(s) | 114, 129, 259, 392, 410 |
| FaqI | GGGAC | 2 cut(s) | 77, 218 |
| FatI | CATG | 3 cut(s) | 257, 390, 408 |
| Fnu4HI | GCNGC | 3 cut(s) | 142, 216, 219 |
| FokI | GGATG | 1 cut(s) | 86 |
| Fsp4HI | GCNGC | 3 cut(s) | 142, 216, 219 |
| GluI | GCNGC | 3 cut(s) | 142, 216, 219 |
| GsaI | CCCAGC | 1 cut(s) | 98 |
| HapII | CCGG | 1 cut(s) | 381 |
| Hin1II | CATG | 3 cut(s) | 261, 394, 412 |
| HinfI | GANTC | 3 cut(s) | 305, 311, 403 |
| HpaII | CCGG | 1 cut(s) | 381 |
| HphI | GGTGA | 1 cut(s) | 395 |
| Hpy188I | TCNGA | 2 cut(s) | 196, 310 |
| Hpy188III | TCNNGA | 1 cut(s) | 161 |
| HpyAV | CCTTC | 3 cut(s) | 34, 195, 391 |
| HpyCH4V | TGCA | 4 cut(s) | 144, 221, 237, 252 |
| Hsp92II | CATG | 3 cut(s) | 261, 394, 412 |
| LpnPI | CCDG | 2 cut(s) | 108, 394 |
| Lsp1109I | GCAGC | 3 cut(s) | 128, 202, 205 |
| LweI | GCATC | 2 cut(s) | 239, 279 |
| MfeI | CAATTG | 1 cut(s) | 169 |
| MluCI | AATT | 1 cut(s) | 169 |
| MlyI | GAGTC | 2 cut(s) | 320, 397 |
| MmeI | TCCRAC | 1 cut(s) | 219 |
| MnlI | CCTC | 4 cut(s) | 49, 76, 115, 289 |
| Mph1103I | ATGCAT | 1 cut(s) | 254 |
| MseI | TTAA | 1 cut(s) | 281 |
| MspI | CCGG | 1 cut(s) | 381 |
| MunI | CAATTG | 1 cut(s) | 169 |
| Mva1269I | GAATGC | 1 cut(s) | 252 |
| NcoI | CCATGG | 1 cut(s) | 257 |
| NlaIII | CATG | 3 cut(s) | 261, 394, 412 |
| NsiI | ATGCAT | 1 cut(s) | 254 |
| NspI | RCATGY | 1 cut(s) | 394 |
| PciI | ACATGT | 1 cut(s) | 390 |
| PctI | GAATGC | 1 cut(s) | 252 |
| PfeI | GAWTC | 1 cut(s) | 305 |
| PinAI | ACCGGT | 1 cut(s) | 380 |
| PkrI | GCNGC | 3 cut(s) | 143, 217, 220 |
| PleI | GAGTC | 2 cut(s) | 319, 397 |
| PpsI | GAGTC | 2 cut(s) | 319, 397 |
| PscI | ACATGT | 1 cut(s) | 390 |
| PspFI | CCCAGC | 1 cut(s) | 94 |
| PstI | CTGCAG | 1 cut(s) | 223 |
| RsaI | GTAC | 1 cut(s) | 31 |
| RsaNI | GTAC | 1 cut(s) | 30 |
| SaqAI | TTAA | 1 cut(s) | 281 |
| SatI | GCNGC | 3 cut(s) | 142, 216, 219 |
| SchI | GAGTC | 2 cut(s) | 320, 397 |
| SetI | ASST | 1 cut(s) | 217 |
| SfaNI | GCATC | 2 cut(s) | 239, 279 |
| SfcI | CTRYAG | 1 cut(s) | 219 |
| Sse9I | AATT | 1 cut(s) | 169 |
| SsiI | CCGC | 2 cut(s) | 133, 320 |
| SspI | AATATT | 1 cut(s) | 269 |
| StyI | CCWWGG | 1 cut(s) | 257 |
| TaqI | TCGA | 2 cut(s) | 162, 395 |
| TaqII | GACCGA | 1 cut(s) | 213 |
| TasI | AATT | 1 cut(s) | 169 |
| TatI | WGTACW | 1 cut(s) | 29 |
| TfiI | GAWTC | 1 cut(s) | 305 |
| Tru1I | TTAA | 1 cut(s) | 281 |
| Tru9I | TTAA | 1 cut(s) | 281 |
| TseI | GCWGC | 3 cut(s) | 141, 215, 218 |
| TspDTI | ATGAA | 2 cut(s) | 93, 359 |
| XceI | RCATGY | 1 cut(s) | 394 |
| Zsp2I | ATGCAT | 1 cut(s) | 254 |