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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 307 |
| AccII | CGCG | 4 cut(s) | 384, 466, 631, 753 |
| AciI | CCGC | 4 cut(s) | 305, 325, 464, 466 |
| AclWI | GGATC | 1 cut(s) | 445 |
| AcoI | YGGCCR | 3 cut(s) | 410, 461, 738 |
| AcsI | RAATTY | 2 cut(s) | 134, 218 |
| AfaI | GTAC | 1 cut(s) | 649 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 557, 736 |
| AflIII | ACRYGT | 2 cut(s) | 382, 597 |
| AgsI | TTSAA | 3 cut(s) | 124, 575, 605 |
| AjnI | CCWGG | 1 cut(s) | 719 |
| AluBI | AGCT | 1 cut(s) | 626 |
| AluI | AGCT | 1 cut(s) | 626 |
| Alw21I | GWGCWC | 1 cut(s) | 473 |
| AlwI | GGATC | 1 cut(s) | 445 |
| Ama87I | CYCGRG | 1 cut(s) | 545 |
| AoxI | GGCC | 4 cut(s) | 410, 461, 727, 738 |
| ApeKI | GCWGC | 1 cut(s) | 626 |
| ApoI | RAATTY | 2 cut(s) | 134, 218 |
| AspLEI | GCGC | 2 cut(s) | 631, 753 |
| AspS9I | GGNCC | 2 cut(s) | 428, 727 |
| AsuHPI | GGTGA | 3 cut(s) | 16, 164, 250 |
| AvaI | CYCGRG | 1 cut(s) | 545 |
| AvaII | GGWCC | 1 cut(s) | 428 |
| BauI | CACGAG | 1 cut(s) | 139 |
| BbsI | GAAGAC | 1 cut(s) | 681 |
| Bbv12I | GWGCWC | 1 cut(s) | 473 |
| BbvI | GCAGC | 1 cut(s) | 613 |
| BccI | CCATC | 1 cut(s) | 530 |
| BceAI | ACGGC | 4 cut(s) | 333, 397, 476, 726 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 544, 578 |
| BciT130I | CCWGG | 1 cut(s) | 721 |
| BfaI | CTAG | 1 cut(s) | 731 |
| BfmI | CTRYAG | 1 cut(s) | 621 |
| BisI | GCNGC | 2 cut(s) | 464, 627 |
| BlsI | GCNGC | 2 cut(s) | 465, 628 |
| Bme1390I | CCNGG | 1 cut(s) | 721 |
| Bme18I | GGWCC | 1 cut(s) | 428 |
| BmeT110I | CYCGRG | 1 cut(s) | 545 |
| BmgT120I | GGNCC | 2 cut(s) | 428, 727 |
| BmiI | GGNNCC | 3 cut(s) | 321, 430, 728 |
| BmrFI | CCNGG | 1 cut(s) | 721 |
| BmsI | GCATC | 1 cut(s) | 239 |
| BpiI | GAAGAC | 1 cut(s) | 681 |
| BpmI | CTGGAG | 1 cut(s) | 742 |
| BsaJI | CCNNGG | 3 cut(s) | 128, 432, 464 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 557, 736 |
| BseBI | CCWGG | 1 cut(s) | 721 |
| BseDI | CCNNGG | 3 cut(s) | 128, 432, 464 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 557, 736 |
| BseMII | CTCAG | 1 cut(s) | 550 |
| BseRI | GAGGAG | 2 cut(s) | 475, 700 |
| BseX3I | CGGCCG | 2 cut(s) | 410, 461 |
| BseXI | GCAGC | 1 cut(s) | 613 |
| Bsh1236I | CGCG | 4 cut(s) | 384, 466, 631, 753 |
| Bsh1285I | CGRYCG | 4 cut(s) | 413, 449, 464, 558 |
| BshFI | GGCC | 4 cut(s) | 412, 463, 729, 740 |
| BsiEI | CGRYCG | 4 cut(s) | 413, 449, 464, 558 |
| BsiHKAI | GWGCWC | 1 cut(s) | 473 |
| BsiHKCI | CYCGRG | 1 cut(s) | 545 |
| BsiWI | CGTACG | 1 cut(s) | 647 |
| BslFI | GGGAC | 1 cut(s) | 414 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 557, 736 |
| BsmFI | GGGAC | 1 cut(s) | 414 |
| BsnI | GGCC | 4 cut(s) | 412, 463, 729, 740 |
| BsoBI | CYCGRG | 1 cut(s) | 545 |
| Bsp1286I | GDGCHC | 1 cut(s) | 473 |
| Bsp143I | GATC | 3 cut(s) | 402, 437, 555 |
| BspACI | CCGC | 4 cut(s) | 305, 325, 464, 466 |
| BspANI | GGCC | 4 cut(s) | 412, 463, 729, 740 |
| BspCNI | CTCAG | 1 cut(s) | 549 |
| BspFNI | CGCG | 4 cut(s) | 384, 466, 631, 753 |
| BspLI | GGNNCC | 3 cut(s) | 321, 430, 728 |
| BspPI | GGATC | 1 cut(s) | 445 |
| BsrBI | CCGCTC | 1 cut(s) | 307 |
| BssECI | CCNNGG | 3 cut(s) | 128, 432, 464 |
| BssMI | GATC | 3 cut(s) | 402, 437, 555 |
| BssSI | CACGAG | 1 cut(s) | 139 |
| BssT1I | CCWWGG | 2 cut(s) | 128, 432 |
| Bst2BI | CACGAG | 1 cut(s) | 139 |
| Bst2UI | CCWGG | 1 cut(s) | 721 |
| Bst4CI | ACNGT | 3 cut(s) | 447, 494, 571 |
| Bst6I | CTCTTC | 1 cut(s) | 108 |
| BstC8I | GCNNGC | 1 cut(s) | 563 |
| BstDEI | CTNAG | 3 cut(s) | 247, 374, 536 |
| BstDSI | CCRYGG | 1 cut(s) | 464 |
| BstFNI | CGCG | 4 cut(s) | 384, 466, 631, 753 |
| BstHHI | GCGC | 2 cut(s) | 631, 753 |
| BstKTI | GATC | 3 cut(s) | 405, 440, 558 |
| BstMBI | GATC | 3 cut(s) | 402, 437, 555 |
| BstMCI | CGRYCG | 4 cut(s) | 413, 449, 464, 558 |
| BstNI | CCWGG | 1 cut(s) | 721 |
| BstNSI | RCATGY | 2 cut(s) | 565, 601 |
| BstSCI | CCNGG | 1 cut(s) | 719 |
| BstSFI | CTRYAG | 1 cut(s) | 621 |
| BstUI | CGCG | 4 cut(s) | 384, 466, 631, 753 |
| BstV1I | GCAGC | 1 cut(s) | 613 |
| BstV2I | GAAGAC | 1 cut(s) | 681 |
| BstX2I | RGATCY | 1 cut(s) | 437 |
| BstYI | RGATCY | 1 cut(s) | 437 |
| BstZI | CGGCCG | 2 cut(s) | 410, 461 |
| BsuRI | GGCC | 4 cut(s) | 412, 463, 729, 740 |
| BtgI | CCRYGG | 1 cut(s) | 464 |
| Cac8I | GCNNGC | 1 cut(s) | 563 |
| CfoI | GCGC | 2 cut(s) | 631, 753 |
| Cfr13I | GGNCC | 2 cut(s) | 428, 727 |
| Cfr42I | CCGCGG | 1 cut(s) | 467 |
| Csp6I | GTAC | 1 cut(s) | 648 |
| CviAII | CATG | 2 cut(s) | 562, 598 |
| CviQI | GTAC | 1 cut(s) | 648 |
| DdeI | CTNAG | 3 cut(s) | 247, 374, 536 |
| DpnI | GATC | 3 cut(s) | 404, 439, 557 |
| DpnII | GATC | 3 cut(s) | 402, 437, 555 |
| EaeI | YGGCCR | 3 cut(s) | 410, 461, 738 |
| EagI | CGGCCG | 2 cut(s) | 410, 461 |
| Eam1104I | CTCTTC | 1 cut(s) | 108 |
| EarI | CTCTTC | 1 cut(s) | 108 |
| EclXI | CGGCCG | 2 cut(s) | 410, 461 |
| Eco130I | CCWWGG | 2 cut(s) | 128, 432 |
| Eco47I | GGWCC | 1 cut(s) | 428 |
| Eco52I | CGGCCG | 2 cut(s) | 410, 461 |
| Eco88I | CYCGRG | 1 cut(s) | 545 |
| EcoO109I | RGGNCCY | 1 cut(s) | 727 |
| EcoRII | CCWGG | 1 cut(s) | 719 |
| EcoT14I | CCWWGG | 2 cut(s) | 128, 432 |
| ErhI | CCWWGG | 2 cut(s) | 128, 432 |
| FaeI | CATG | 2 cut(s) | 565, 601 |
| FaiI | YATR | 4 cut(s) | 500, 563, 599, 623 |
| FaqI | GGGAC | 1 cut(s) | 414 |
| FatI | CATG | 2 cut(s) | 561, 597 |
| FauI | CCCGC | 1 cut(s) | 332 |
| Fnu4HI | GCNGC | 2 cut(s) | 464, 627 |
| Fsp4HI | GCNGC | 2 cut(s) | 464, 627 |
| FspBI | CTAG | 1 cut(s) | 731 |
| GlaI | GCGC | 2 cut(s) | 630, 752 |
| GluI | GCNGC | 2 cut(s) | 464, 627 |
| GsuI | CTGGAG | 1 cut(s) | 742 |
| HaeIII | GGCC | 4 cut(s) | 412, 463, 729, 740 |
| HhaI | GCGC | 2 cut(s) | 631, 753 |
| Hin1II | CATG | 2 cut(s) | 565, 601 |
| Hin6I | GCGC | 2 cut(s) | 629, 751 |
| HinP1I | GCGC | 2 cut(s) | 629, 751 |
| HinfI | GANTC | 4 cut(s) | 155, 288, 355, 454 |
| HphI | GGTGA | 3 cut(s) | 16, 164, 250 |
| Hpy166II | GTNNAC | 2 cut(s) | 7, 388 |
| Hpy188I | TCNGA | 1 cut(s) | 160 |
| Hpy188III | TCNNGA | 5 cut(s) | 23, 55, 139, 262, 545 |
| Hpy8I | GTNNAC | 2 cut(s) | 7, 388 |
| Hpy99I | CGWCG | 1 cut(s) | 342 |
| HpyAV | CCTTC | 3 cut(s) | 61, 235, 415 |
| HpyCH4III | ACNGT | 3 cut(s) | 447, 494, 571 |
| HpyCH4IV | ACGT | 1 cut(s) | 650 |
| HpyCH4V | TGCA | 2 cut(s) | 230, 364 |
| HpyF3I | CTNAG | 3 cut(s) | 247, 374, 536 |
| HpySE526I | ACGT | 1 cut(s) | 650 |
| Hsp92II | CATG | 2 cut(s) | 565, 601 |
| HspAI | GCGC | 2 cut(s) | 629, 751 |
| KspI | CCGCGG | 1 cut(s) | 467 |
| Kzo9I | GATC | 3 cut(s) | 402, 437, 555 |
| LmnI | GCTCC | 2 cut(s) | 271, 325 |
| LpnPI | CCDG | 2 cut(s) | 706, 733 |
| Lsp1109I | GCAGC | 1 cut(s) | 613 |
| LweI | GCATC | 1 cut(s) | 239 |
| MaeI | CTAG | 1 cut(s) | 731 |
| MaeII | ACGT | 1 cut(s) | 650 |
| MalI | GATC | 3 cut(s) | 404, 439, 557 |
| MbiI | CCGCTC | 1 cut(s) | 307 |
| MboI | GATC | 3 cut(s) | 402, 437, 555 |
| MboII | GAAGA | 5 cut(s) | 125, 432, 463, 587, 686 |
| MflI | RGATCY | 1 cut(s) | 437 |
| MhlI | GDGCHC | 1 cut(s) | 473 |
| MluCI | AATT | 4 cut(s) | 99, 134, 218, 266 |
| MluI | ACGCGT | 1 cut(s) | 382 |
| MlyI | GAGTC | 1 cut(s) | 282 |
| MseI | TTAA | 2 cut(s) | 107, 746 |
| MslI | CAYNNNNRTG | 1 cut(s) | 746 |
| MspA1I | CMGCKG | 1 cut(s) | 466 |
| MspR9I | CCNGG | 1 cut(s) | 721 |
| MvaI | CCWGG | 1 cut(s) | 721 |
| MvnI | CGCG | 4 cut(s) | 384, 466, 631, 753 |
| NdeII | GATC | 3 cut(s) | 402, 437, 555 |
| NlaIII | CATG | 2 cut(s) | 565, 601 |
| NlaIV | GGNNCC | 3 cut(s) | 321, 430, 728 |
| NspI | RCATGY | 2 cut(s) | 565, 601 |
| PaeI | GCATGC | 1 cut(s) | 565 |
| PciI | ACATGT | 1 cut(s) | 597 |
| PfeI | GAWTC | 3 cut(s) | 155, 355, 454 |
| Pfl23II | CGTACG | 1 cut(s) | 647 |
| PfoI | TCCNGGA | 1 cut(s) | 719 |
| PkrI | GCNGC | 2 cut(s) | 465, 628 |
| Ple19I | CGATCG | 1 cut(s) | 558 |
| PleI | GAGTC | 1 cut(s) | 282 |
| PpsI | GAGTC | 1 cut(s) | 282 |
| PscI | ACATGT | 1 cut(s) | 597 |
| Psp6I | CCWGG | 1 cut(s) | 719 |
| PspGI | CCWGG | 1 cut(s) | 719 |
| PspLI | CGTACG | 1 cut(s) | 647 |
| PspN4I | GGNNCC | 3 cut(s) | 321, 430, 728 |
| PspPI | GGNCC | 2 cut(s) | 428, 727 |
| PsuI | RGATCY | 1 cut(s) | 437 |
| PvuI | CGATCG | 1 cut(s) | 558 |
| RsaI | GTAC | 1 cut(s) | 649 |
| RsaNI | GTAC | 1 cut(s) | 648 |
| RseI | CAYNNNNRTG | 1 cut(s) | 746 |
| SacII | CCGCGG | 1 cut(s) | 467 |
| SaqAI | TTAA | 2 cut(s) | 107, 746 |
| SatI | GCNGC | 2 cut(s) | 464, 627 |
| Sau3AI | GATC | 3 cut(s) | 402, 437, 555 |
| Sau96I | GGNCC | 2 cut(s) | 428, 727 |
| SchI | GAGTC | 1 cut(s) | 282 |
| ScrFI | CCNGG | 1 cut(s) | 721 |
| SduI | GDGCHC | 1 cut(s) | 473 |
| SetI | ASST | 9 cut(s) | 154, 298, 333, 426, 485, 521, 628, 653, 746 |
| SfaNI | GCATC | 1 cut(s) | 239 |
| SfcI | CTRYAG | 1 cut(s) | 621 |
| Sfr303I | CCGCGG | 1 cut(s) | 467 |
| SgrBI | CCGCGG | 1 cut(s) | 467 |
| SinI | GGWCC | 1 cut(s) | 428 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 746 |
| SphI | GCATGC | 1 cut(s) | 565 |
| Sse9I | AATT | 4 cut(s) | 99, 134, 218, 266 |
| SsiI | CCGC | 4 cut(s) | 305, 325, 464, 466 |
| SspMI | CTAG | 1 cut(s) | 731 |
| StyD4I | CCNGG | 1 cut(s) | 719 |
| StyI | CCWWGG | 2 cut(s) | 128, 432 |
| TaaI | ACNGT | 3 cut(s) | 447, 494, 571 |
| TaiI | ACGT | 1 cut(s) | 653 |
| TaqI | TCGA | 7 cut(s) | 22, 54, 82, 298, 337, 449, 513 |
| TaqII | GACCGA | 1 cut(s) | 416 |
| TasI | AATT | 4 cut(s) | 99, 134, 218, 266 |
| TauI | GCSGC | 1 cut(s) | 466 |
| TfiI | GAWTC | 3 cut(s) | 155, 355, 454 |
| Tru1I | TTAA | 2 cut(s) | 107, 746 |
| Tru9I | TTAA | 2 cut(s) | 107, 746 |
| TseI | GCWGC | 1 cut(s) | 626 |
| TspDTI | ATGAA | 1 cut(s) | 368 |
| VpaK11BI | GGWCC | 1 cut(s) | 428 |
| XapI | RAATTY | 2 cut(s) | 134, 218 |
| XceI | RCATGY | 2 cut(s) | 565, 601 |
| XspI | CTAG | 1 cut(s) | 731 |