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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 194 |
| AclWI | GGATC | 3 cut(s) | 42, 180, 425 |
| AcsI | RAATTY | 3 cut(s) | 112, 410, 686 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 95, 215, 364, 461 |
| AgsI | TTSAA | 2 cut(s) | 438, 528 |
| AjnI | CCWGG | 2 cut(s) | 88, 660 |
| AluBI | AGCT | 3 cut(s) | 161, 187, 566 |
| AluI | AGCT | 3 cut(s) | 161, 187, 566 |
| Alw26I | GTCTC | 2 cut(s) | 62, 107 |
| AlwI | GGATC | 3 cut(s) | 42, 180, 425 |
| AoxI | GGCC | 2 cut(s) | 15, 366 |
| ApoI | RAATTY | 3 cut(s) | 112, 410, 686 |
| AseI | ATTAAT | 1 cut(s) | 642 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 410 |
| AspA2I | CCTAGG | 1 cut(s) | 445 |
| AspLEI | GCGC | 1 cut(s) | 580 |
| AspS9I | GGNCC | 1 cut(s) | 472 |
| AsuC2I | CCSGG | 1 cut(s) | 170 |
| AsuHPI | GGTGA | 2 cut(s) | 264, 476 |
| AvaII | GGWCC | 1 cut(s) | 472 |
| AvrII | CCTAGG | 1 cut(s) | 445 |
| AxyI | CCTNAGG | 1 cut(s) | 210 |
| BanI | GGYRCC | 1 cut(s) | 194 |
| BceAI | ACGGC | 1 cut(s) | 539 |
| BciT130I | CCWGG | 2 cut(s) | 90, 662 |
| BcnI | CCSGG | 1 cut(s) | 170 |
| BcoDI | GTCTC | 2 cut(s) | 62, 107 |
| BfaI | CTAG | 1 cut(s) | 446 |
| BfmI | CTRYAG | 1 cut(s) | 362 |
| BlnI | CCTAGG | 1 cut(s) | 445 |
| Bme1390I | CCNGG | 3 cut(s) | 90, 170, 662 |
| Bme18I | GGWCC | 1 cut(s) | 472 |
| BmgT120I | GGNCC | 1 cut(s) | 472 |
| BmiI | GGNNCC | 3 cut(s) | 84, 196, 474 |
| BmrFI | CCNGG | 3 cut(s) | 90, 170, 662 |
| BmsI | GCATC | 3 cut(s) | 243, 283, 565 |
| BpmI | CTGGAG | 1 cut(s) | 72 |
| BpuMI | CCSGG | 1 cut(s) | 170 |
| BsaJI | CCNNGG | 4 cut(s) | 198, 301, 445, 555 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 95, 215, 364, 461 |
| Bse21I | CCTNAGG | 1 cut(s) | 210 |
| Bse3DI | GCAATG | 1 cut(s) | 243 |
| BseBI | CCWGG | 2 cut(s) | 90, 662 |
| BseDI | CCNNGG | 4 cut(s) | 198, 301, 445, 555 |
| BseGI | GGATG | 2 cut(s) | 104, 441 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 95, 215, 364, 461 |
| BseMI | GCAATG | 1 cut(s) | 243 |
| BseMII | CTCAG | 2 cut(s) | 224, 477 |
| BseRI | GAGGAG | 2 cut(s) | 332, 347 |
| BsgI | GTGCAG | 1 cut(s) | 80 |
| BshFI | GGCC | 2 cut(s) | 17, 368 |
| BshNI | GGYRCC | 1 cut(s) | 194 |
| BsiSI | CCGG | 2 cut(s) | 169, 651 |
| BslFI | GGGAC | 1 cut(s) | 458 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 95, 215, 364, 461 |
| BsmAI | GTCTC | 2 cut(s) | 62, 107 |
| BsmFI | GGGAC | 1 cut(s) | 458 |
| BsmI | GAATGC | 2 cut(s) | 296, 456 |
| BsnI | GGCC | 2 cut(s) | 17, 368 |
| Bsp143I | GATC | 3 cut(s) | 34, 172, 417 |
| Bsp19I | CCATGG | 1 cut(s) | 301 |
| BspANI | GGCC | 2 cut(s) | 17, 368 |
| BspCNI | CTCAG | 2 cut(s) | 223, 478 |
| BspLI | GGNNCC | 3 cut(s) | 84, 196, 474 |
| BspMAI | CTGCAG | 1 cut(s) | 366 |
| BspPI | GGATC | 3 cut(s) | 42, 180, 425 |
| BspT107I | GGYRCC | 1 cut(s) | 194 |
| BsrDI | GCAATG | 1 cut(s) | 243 |
| BssECI | CCNNGG | 4 cut(s) | 198, 301, 445, 555 |
| BssMI | GATC | 3 cut(s) | 34, 172, 417 |
| BssT1I | CCWWGG | 3 cut(s) | 198, 301, 445 |
| Bst2UI | CCWGG | 2 cut(s) | 90, 662 |
| Bst6I | CTCTTC | 1 cut(s) | 395 |
| BstC8I | GCNNGC | 3 cut(s) | 185, 366, 680 |
| BstDEI | CTNAG | 3 cut(s) | 210, 267, 486 |
| BstDSI | CCRYGG | 2 cut(s) | 301, 555 |
| BstEII | GGTNACC | 1 cut(s) | 218 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 362 |
| BstF5I | GGATG | 2 cut(s) | 104, 441 |
| BstHHI | GCGC | 1 cut(s) | 580 |
| BstKTI | GATC | 3 cut(s) | 37, 175, 420 |
| BstMAI | GTCTC | 2 cut(s) | 62, 107 |
| BstMBI | GATC | 3 cut(s) | 34, 172, 417 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 577 |
| BstNI | CCWGG | 2 cut(s) | 90, 662 |
| BstPI | GGTNACC | 1 cut(s) | 218 |
| BstSCI | CCNGG | 3 cut(s) | 88, 168, 660 |
| BstSFI | CTRYAG | 1 cut(s) | 362 |
| BstX2I | RGATCY | 1 cut(s) | 417 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 55 |
| BstYI | RGATCY | 1 cut(s) | 417 |
| Bsu36I | CCTNAGG | 1 cut(s) | 210 |
| BsuRI | GGCC | 2 cut(s) | 17, 368 |
| BtgI | CCRYGG | 2 cut(s) | 301, 555 |
| BtsCI | GGATG | 2 cut(s) | 104, 441 |
| Cac8I | GCNNGC | 3 cut(s) | 185, 366, 680 |
| CfoI | GCGC | 1 cut(s) | 580 |
| Cfr13I | GGNCC | 1 cut(s) | 472 |
| CseI | GACGC | 1 cut(s) | 131 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 613, 648 |
| CviAII | CATG | 4 cut(s) | 38, 261, 302, 377 |
| CviJI | RGCY | 9 cut(s) | 17, 161, 187, 309, 329, 368, 554, 566, 678 |
| CviKI_1 | RGCY | 9 cut(s) | 17, 161, 187, 309, 329, 368, 554, 566, 678 |
| DdeI | CTNAG | 3 cut(s) | 210, 267, 486 |
| DpnI | GATC | 3 cut(s) | 36, 174, 419 |
| DpnII | GATC | 3 cut(s) | 34, 172, 417 |
| Eam1104I | CTCTTC | 1 cut(s) | 395 |
| EarI | CTCTTC | 1 cut(s) | 395 |
| Eco130I | CCWWGG | 3 cut(s) | 198, 301, 445 |
| Eco147I | AGGCCT | 1 cut(s) | 368 |
| Eco47I | GGWCC | 1 cut(s) | 472 |
| Eco81I | CCTNAGG | 1 cut(s) | 210 |
| Eco91I | GGTNACC | 1 cut(s) | 218 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 362 |
| EcoO65I | GGTNACC | 1 cut(s) | 218 |
| EcoRII | CCWGG | 2 cut(s) | 88, 660 |
| EcoT14I | CCWWGG | 3 cut(s) | 198, 301, 445 |
| EcoT22I | ATGCAT | 1 cut(s) | 298 |
| ErhI | CCWWGG | 3 cut(s) | 198, 301, 445 |
| FaeI | CATG | 4 cut(s) | 41, 264, 305, 380 |
| FaiI | YATR | 8 cut(s) | 39, 95, 262, 303, 314, 378, 496, 533 |
| FaqI | GGGAC | 1 cut(s) | 458 |
| FatI | CATG | 4 cut(s) | 37, 260, 301, 376 |
| FokI | GGATG | 2 cut(s) | 111, 428 |
| FspBI | CTAG | 1 cut(s) | 446 |
| GlaI | GCGC | 1 cut(s) | 579 |
| GsuI | CTGGAG | 1 cut(s) | 72 |
| HaeIII | GGCC | 2 cut(s) | 17, 368 |
| HapII | CCGG | 2 cut(s) | 169, 651 |
| HgaI | GACGC | 1 cut(s) | 131 |
| HhaI | GCGC | 1 cut(s) | 580 |
| Hin1II | CATG | 4 cut(s) | 41, 264, 305, 380 |
| Hin6I | GCGC | 1 cut(s) | 578 |
| HinP1I | GCGC | 1 cut(s) | 578 |
| HindIII | AAGCTT | 1 cut(s) | 185 |
| HinfI | GANTC | 2 cut(s) | 242, 589 |
| HpaII | CCGG | 2 cut(s) | 169, 651 |
| HphI | GGTGA | 2 cut(s) | 264, 476 |
| Hpy188I | TCNGA | 5 cut(s) | 34, 137, 340, 487, 505 |
| Hpy188III | TCNNGA | 2 cut(s) | 415, 421 |
| Hpy99I | CGWCG | 1 cut(s) | 125 |
| HpyAV | CCTTC | 2 cut(s) | 233, 613 |
| HpyCH4V | TGCA | 8 cut(s) | 4, 12, 61, 74, 296, 364, 494, 571 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 577 |
| HpyF3I | CTNAG | 3 cut(s) | 210, 267, 486 |
| Hsp92II | CATG | 4 cut(s) | 41, 264, 305, 380 |
| HspAI | GCGC | 1 cut(s) | 578 |
| Kzo9I | GATC | 3 cut(s) | 34, 172, 417 |
| LmnI | GCTCC | 1 cut(s) | 158 |
| LweI | GCATC | 3 cut(s) | 243, 283, 565 |
| MaeI | CTAG | 1 cut(s) | 446 |
| MaeIII | GTNAC | 2 cut(s) | 218, 270 |
| MalI | GATC | 3 cut(s) | 36, 174, 419 |
| MboI | GATC | 3 cut(s) | 34, 172, 417 |
| MboII | GAAGA | 3 cut(s) | 382, 385, 628 |
| MflI | RGATCY | 1 cut(s) | 417 |
| MluCI | AATT | 7 cut(s) | 49, 107, 112, 153, 410, 540, 686 |
| MlyI | GAGTC | 1 cut(s) | 251 |
| MmeI | TCCRAC | 1 cut(s) | 617 |
| MnlI | CCTC | 7 cut(s) | 57, 96, 219, 310, 368, 398, 481 |
| Mph1103I | ATGCAT | 1 cut(s) | 298 |
| MroXI | GAANNNNTTC | 1 cut(s) | 410 |
| MseI | TTAA | 5 cut(s) | 110, 117, 189, 543, 642 |
| MslI | CAYNNNNRTG | 1 cut(s) | 381 |
| MspI | CCGG | 2 cut(s) | 169, 651 |
| MspR9I | CCNGG | 3 cut(s) | 90, 170, 662 |
| Mva1269I | GAATGC | 2 cut(s) | 296, 456 |
| MvaI | CCWGG | 2 cut(s) | 90, 662 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 577 |
| NciI | CCSGG | 1 cut(s) | 170 |
| NcoI | CCATGG | 1 cut(s) | 301 |
| NdeII | GATC | 3 cut(s) | 34, 172, 417 |
| NlaIII | CATG | 4 cut(s) | 41, 264, 305, 380 |
| NlaIV | GGNNCC | 3 cut(s) | 84, 196, 474 |
| NmuCI | GTSAC | 1 cut(s) | 270 |
| NsiI | ATGCAT | 1 cut(s) | 298 |
| PceI | AGGCCT | 1 cut(s) | 368 |
| PctI | GAATGC | 2 cut(s) | 296, 456 |
| PdmI | GAANNNNTTC | 1 cut(s) | 410 |
| PfeI | GAWTC | 1 cut(s) | 589 |
| PflFI | GACNNNGTC | 1 cut(s) | 509 |
| PfoI | TCCNGGA | 1 cut(s) | 168 |
| PleI | GAGTC | 1 cut(s) | 250 |
| PpsI | GAGTC | 1 cut(s) | 250 |
| PshBI | ATTAAT | 1 cut(s) | 642 |
| Psp6I | CCWGG | 2 cut(s) | 88, 660 |
| PspEI | GGTNACC | 1 cut(s) | 218 |
| PspGI | CCWGG | 2 cut(s) | 88, 660 |
| PspN4I | GGNNCC | 3 cut(s) | 84, 196, 474 |
| PspPI | GGNCC | 1 cut(s) | 472 |
| PstI | CTGCAG | 1 cut(s) | 366 |
| PsuI | RGATCY | 1 cut(s) | 417 |
| PsyI | GACNNNGTC | 1 cut(s) | 509 |
| RseI | CAYNNNNRTG | 1 cut(s) | 381 |
| SaqAI | TTAA | 5 cut(s) | 110, 117, 189, 543, 642 |
| Sau3AI | GATC | 3 cut(s) | 34, 172, 417 |
| Sau96I | GGNCC | 1 cut(s) | 472 |
| SbfI | CCTGCAGG | 1 cut(s) | 366 |
| SchI | GAGTC | 1 cut(s) | 251 |
| ScrFI | CCNGG | 3 cut(s) | 90, 170, 662 |
| SdaI | CCTGCAGG | 1 cut(s) | 366 |
| SfaNI | GCATC | 3 cut(s) | 243, 283, 565 |
| SfcI | CTRYAG | 1 cut(s) | 362 |
| SinI | GGWCC | 1 cut(s) | 472 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 381 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 366 |
| Sse9I | AATT | 7 cut(s) | 49, 107, 112, 153, 410, 540, 686 |
| SseBI | AGGCCT | 1 cut(s) | 368 |
| SspI | AATATT | 1 cut(s) | 286 |
| SspMI | CTAG | 1 cut(s) | 446 |
| StuI | AGGCCT | 1 cut(s) | 368 |
| StyD4I | CCNGG | 3 cut(s) | 88, 168, 660 |
| StyI | CCWWGG | 3 cut(s) | 198, 301, 445 |
| TaqI | TCGA | 2 cut(s) | 291, 422 |
| TasI | AATT | 7 cut(s) | 49, 107, 112, 153, 410, 540, 686 |
| TfiI | GAWTC | 1 cut(s) | 589 |
| Tru1I | TTAA | 5 cut(s) | 110, 117, 189, 543, 642 |
| Tru9I | TTAA | 5 cut(s) | 110, 117, 189, 543, 642 |
| TseFI | GTSAC | 1 cut(s) | 270 |
| Tsp45I | GTSAC | 1 cut(s) | 270 |
| TspDTI | ATGAA | 3 cut(s) | 249, 337, 393 |
| Tth111I | GACNNNGTC | 1 cut(s) | 509 |
| VpaK11BI | GGWCC | 1 cut(s) | 472 |
| VspI | ATTAAT | 1 cut(s) | 642 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 362 |
| XapI | RAATTY | 3 cut(s) | 112, 410, 686 |
| XmaJI | CCTAGG | 1 cut(s) | 445 |
| XmnI | GAANNNNTTC | 1 cut(s) | 410 |
| XspI | CTAG | 1 cut(s) | 446 |
| Zsp2I | ATGCAT | 1 cut(s) | 298 |