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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 320 |
| AcoI | YGGCCR | 4 cut(s) | 242, 295, 361, 373 |
| AgsI | TTSAA | 3 cut(s) | 50, 61, 97 |
| AluBI | AGCT | 1 cut(s) | 130 |
| AluI | AGCT | 1 cut(s) | 130 |
| AoxI | GGCC | 6 cut(s) | 99, 242, 295, 331, 361, 373 |
| AspS9I | GGNCC | 1 cut(s) | 332 |
| AsuHPI | GGTGA | 5 cut(s) | 242, 283, 295, 319, 349 |
| BalI | TGGCCA | 1 cut(s) | 375 |
| BccI | CCATC | 2 cut(s) | 133, 193 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 241 |
| BmgT120I | GGNCC | 1 cut(s) | 332 |
| BmsI | GCATC | 3 cut(s) | 55, 103, 196 |
| BsaWI | WCCGGW | 1 cut(s) | 276 |
| Bse118I | RCCGGY | 3 cut(s) | 293, 329, 359 |
| BseGI | GGATG | 2 cut(s) | 181, 211 |
| Bsh1236I | CGCG | 1 cut(s) | 320 |
| BshFI | GGCC | 6 cut(s) | 101, 244, 297, 333, 363, 375 |
| BsiSI | CCGG | 4 cut(s) | 277, 294, 330, 360 |
| BsnI | GGCC | 6 cut(s) | 101, 244, 297, 333, 363, 375 |
| BspANI | GGCC | 6 cut(s) | 101, 244, 297, 333, 363, 375 |
| BspFNI | CGCG | 1 cut(s) | 320 |
| BsrFI | RCCGGY | 3 cut(s) | 293, 329, 359 |
| BssAI | RCCGGY | 3 cut(s) | 293, 329, 359 |
| Bst4CI | ACNGT | 2 cut(s) | 254, 307 |
| Bst6I | CTCTTC | 1 cut(s) | 45 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 215 |
| BstC8I | GCNNGC | 1 cut(s) | 186 |
| BstF5I | GGATG | 2 cut(s) | 181, 211 |
| BstFNI | CGCG | 1 cut(s) | 320 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 215, 241 |
| BstUI | CGCG | 1 cut(s) | 320 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 342, 371 |
| BsuRI | GGCC | 6 cut(s) | 101, 244, 297, 333, 363, 375 |
| BtsCI | GGATG | 2 cut(s) | 181, 211 |
| BtsI | GCAGTG | 1 cut(s) | 104 |
| BtsIMutI | CAGTG | 1 cut(s) | 104 |
| Cac8I | GCNNGC | 1 cut(s) | 186 |
| Cfr10I | RCCGGY | 3 cut(s) | 293, 329, 359 |
| Cfr13I | GGNCC | 1 cut(s) | 332 |
| CviAII | CATG | 3 cut(s) | 258, 311, 377 |
| CviJI | RGCY | 8 cut(s) | 101, 130, 235, 244, 297, 333, 363, 375 |
| CviKI_1 | RGCY | 8 cut(s) | 101, 130, 235, 244, 297, 333, 363, 375 |
| EaeI | YGGCCR | 4 cut(s) | 242, 295, 361, 373 |
| Eam1104I | CTCTTC | 1 cut(s) | 45 |
| EarI | CTCTTC | 1 cut(s) | 45 |
| Eco147I | AGGCCT | 1 cut(s) | 101 |
| FaeI | CATG | 3 cut(s) | 261, 314, 380 |
| FaiI | YATR | 4 cut(s) | 259, 312, 324, 378 |
| FatI | CATG | 3 cut(s) | 257, 310, 376 |
| FokI | GGATG | 2 cut(s) | 188, 218 |
| HaeIII | GGCC | 6 cut(s) | 101, 244, 297, 333, 363, 375 |
| HapII | CCGG | 4 cut(s) | 277, 294, 330, 360 |
| Hin1II | CATG | 3 cut(s) | 261, 314, 380 |
| HpaII | CCGG | 4 cut(s) | 277, 294, 330, 360 |
| HphI | GGTGA | 5 cut(s) | 242, 283, 295, 319, 349 |
| Hpy99I | CGWCG | 1 cut(s) | 31 |
| HpyAV | CCTTC | 1 cut(s) | 91 |
| HpyCH4III | ACNGT | 2 cut(s) | 254, 307 |
| HpyCH4IV | ACGT | 1 cut(s) | 121 |
| HpyCH4V | TGCA | 4 cut(s) | 109, 184, 209, 218 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 215, 241 |
| HpySE526I | ACGT | 1 cut(s) | 121 |
| Hsp92II | CATG | 3 cut(s) | 261, 314, 380 |
| LpnPI | CCDG | 4 cut(s) | 290, 307, 343, 373 |
| LweI | GCATC | 3 cut(s) | 55, 103, 196 |
| MaeII | ACGT | 1 cut(s) | 121 |
| MaeIII | GTNAC | 7 cut(s) | 248, 260, 272, 289, 301, 325, 355 |
| MboII | GAAGA | 2 cut(s) | 43, 62 |
| MlsI | TGGCCA | 1 cut(s) | 375 |
| MluNI | TGGCCA | 1 cut(s) | 375 |
| MmeI | TCCRAC | 1 cut(s) | 137 |
| MnlI | CCTC | 4 cut(s) | 112, 166, 196, 246 |
| Mox20I | TGGCCA | 1 cut(s) | 375 |
| MscI | TGGCCA | 1 cut(s) | 375 |
| MseI | TTAA | 1 cut(s) | 387 |
| Msp20I | TGGCCA | 1 cut(s) | 375 |
| MspI | CCGG | 4 cut(s) | 277, 294, 330, 360 |
| MvnI | CGCG | 1 cut(s) | 320 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 215, 241 |
| NlaIII | CATG | 3 cut(s) | 261, 314, 380 |
| NmuCI | GTSAC | 5 cut(s) | 248, 289, 301, 325, 355 |
| PceI | AGGCCT | 1 cut(s) | 101 |
| PspPI | GGNCC | 1 cut(s) | 332 |
| SaqAI | TTAA | 1 cut(s) | 387 |
| Sau96I | GGNCC | 1 cut(s) | 332 |
| SetI | ASST | 2 cut(s) | 124, 132 |
| SfaNI | GCATC | 3 cut(s) | 55, 103, 196 |
| SseBI | AGGCCT | 1 cut(s) | 101 |
| StuI | AGGCCT | 1 cut(s) | 101 |
| TaaI | ACNGT | 2 cut(s) | 254, 307 |
| TaiI | ACGT | 1 cut(s) | 124 |
| TaqI | TCGA | 1 cut(s) | 132 |
| Tru1I | TTAA | 1 cut(s) | 387 |
| Tru9I | TTAA | 1 cut(s) | 387 |
| TscAI | CASTG | 1 cut(s) | 111 |
| TseFI | GTSAC | 5 cut(s) | 248, 289, 301, 325, 355 |
| Tsp45I | GTSAC | 5 cut(s) | 248, 289, 301, 325, 355 |
| TspRI | CASTG | 1 cut(s) | 111 |