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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 236 |
| AfaI | GTAC | 3 cut(s) | 486, 704, 746 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 84, 230 |
| AgsI | TTSAA | 4 cut(s) | 104, 344, 635, 674 |
| AjiI | CACGTC | 1 cut(s) | 38 |
| AjnI | CCWGG | 1 cut(s) | 40 |
| AluBI | AGCT | 5 cut(s) | 122, 289, 386, 590, 694 |
| AluI | AGCT | 5 cut(s) | 122, 289, 386, 590, 694 |
| Alw26I | GTCTC | 1 cut(s) | 473 |
| AoxI | GGCC | 1 cut(s) | 442 |
| ApeKI | GCWGC | 2 cut(s) | 122, 598 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 660, 692 |
| AspLEI | GCGC | 1 cut(s) | 236 |
| AsuC2I | CCSGG | 1 cut(s) | 231 |
| AsuHPI | GGTGA | 3 cut(s) | 56, 87, 306 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 281, 314 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 273, 305 |
| BbvI | GCAGC | 2 cut(s) | 109, 610 |
| BccI | CCATC | 1 cut(s) | 163 |
| BciT130I | CCWGG | 1 cut(s) | 42 |
| BcnI | CCSGG | 1 cut(s) | 231 |
| BcoDI | GTCTC | 1 cut(s) | 473 |
| BisI | GCNGC | 2 cut(s) | 123, 599 |
| BlsI | GCNGC | 2 cut(s) | 124, 600 |
| BmcAI | AGTACT | 1 cut(s) | 746 |
| Bme1390I | CCNGG | 2 cut(s) | 42, 231 |
| BmgBI | CACGTC | 1 cut(s) | 38 |
| BmrFI | CCNGG | 2 cut(s) | 42, 231 |
| BmsI | GCATC | 2 cut(s) | 97, 166 |
| BpuMI | CCSGG | 1 cut(s) | 231 |
| BsaJI | CCNNGG | 1 cut(s) | 82 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 235, 265 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 84, 230 |
| Bse1I | ACTGG | 1 cut(s) | 752 |
| BseBI | CCWGG | 1 cut(s) | 42 |
| BseDI | CCNNGG | 1 cut(s) | 82 |
| BseGI | GGATG | 1 cut(s) | 646 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 84, 230 |
| BseMII | CTCAG | 2 cut(s) | 378, 444 |
| BseNI | ACTGG | 1 cut(s) | 752 |
| BseRI | GAGGAG | 2 cut(s) | 54, 57 |
| BseXI | GCAGC | 2 cut(s) | 109, 610 |
| BsgI | GTGCAG | 1 cut(s) | 108 |
| Bsh1236I | CGCG | 1 cut(s) | 236 |
| Bsh1285I | CGRYCG | 1 cut(s) | 82 |
| BshFI | GGCC | 1 cut(s) | 444 |
| BsiEI | CGRYCG | 1 cut(s) | 82 |
| BsiSI | CCGG | 1 cut(s) | 230 |
| BslFI | GGGAC | 1 cut(s) | 527 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 84, 230 |
| BsmAI | GTCTC | 1 cut(s) | 473 |
| BsmFI | GGGAC | 1 cut(s) | 527 |
| BsmI | GAATGC | 1 cut(s) | 110 |
| BsnI | GGCC | 1 cut(s) | 444 |
| Bsp1407I | TGTACA | 1 cut(s) | 702 |
| Bsp143I | GATC | 1 cut(s) | 303 |
| BspANI | GGCC | 1 cut(s) | 444 |
| BspCNI | CTCAG | 3 cut(s) | 4, 379, 443 |
| BspFNI | CGCG | 1 cut(s) | 236 |
| BsrGI | TGTACA | 1 cut(s) | 702 |
| BsrI | ACTGG | 1 cut(s) | 752 |
| BssECI | CCNNGG | 1 cut(s) | 82 |
| BssMI | GATC | 1 cut(s) | 303 |
| Bst2UI | CCWGG | 1 cut(s) | 42 |
| Bst4CI | ACNGT | 2 cut(s) | 484, 702 |
| Bst6I | CTCTTC | 1 cut(s) | 464 |
| BstAUI | TGTACA | 1 cut(s) | 702 |
| BstC8I | GCNNGC | 1 cut(s) | 181 |
| BstDEI | CTNAG | 3 cut(s) | 12, 387, 430 |
| BstF5I | GGATG | 1 cut(s) | 646 |
| BstFNI | CGCG | 1 cut(s) | 236 |
| BstHHI | GCGC | 1 cut(s) | 236 |
| BstKTI | GATC | 1 cut(s) | 306 |
| BstMAI | GTCTC | 1 cut(s) | 473 |
| BstMBI | GATC | 1 cut(s) | 303 |
| BstMCI | CGRYCG | 1 cut(s) | 82 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 119, 604 |
| BstNI | CCWGG | 1 cut(s) | 42 |
| BstNSI | RCATGY | 1 cut(s) | 721 |
| BstSCI | CCNGG | 2 cut(s) | 40, 229 |
| BstUI | CGCG | 1 cut(s) | 236 |
| BstV1I | GCAGC | 2 cut(s) | 109, 610 |
| BsuRI | GGCC | 1 cut(s) | 444 |
| BtrI | CACGTC | 1 cut(s) | 38 |
| BtsCI | GGATG | 1 cut(s) | 646 |
| BtsIMutI | CAGTG | 1 cut(s) | 563 |
| Cac8I | GCNNGC | 1 cut(s) | 181 |
| CfoI | GCGC | 1 cut(s) | 236 |
| Csp6I | GTAC | 3 cut(s) | 485, 703, 745 |
| CviAII | CATG | 2 cut(s) | 620, 718 |
| CviJI | RGCY | 9 cut(s) | 122, 289, 328, 386, 400, 444, 590, 601, 694 |
| CviKI_1 | RGCY | 9 cut(s) | 122, 289, 328, 386, 400, 444, 590, 601, 694 |
| CviQI | GTAC | 3 cut(s) | 485, 703, 745 |
| DdeI | CTNAG | 3 cut(s) | 12, 387, 430 |
| DpnI | GATC | 1 cut(s) | 305 |
| DpnII | GATC | 1 cut(s) | 303 |
| Eam1104I | CTCTTC | 1 cut(s) | 464 |
| EarI | CTCTTC | 1 cut(s) | 464 |
| EcoRII | CCWGG | 1 cut(s) | 40 |
| EcoT22I | ATGCAT | 1 cut(s) | 211 |
| FaeI | CATG | 2 cut(s) | 623, 721 |
| FaqI | GGGAC | 1 cut(s) | 527 |
| FatI | CATG | 2 cut(s) | 619, 717 |
| FauNDI | CATATG | 1 cut(s) | 366 |
| Fnu4HI | GCNGC | 2 cut(s) | 123, 599 |
| FokI | GGATG | 1 cut(s) | 653 |
| Fsp4HI | GCNGC | 2 cut(s) | 123, 599 |
| GlaI | GCGC | 1 cut(s) | 235 |
| GluI | GCNGC | 2 cut(s) | 123, 599 |
| HaeIII | GGCC | 1 cut(s) | 444 |
| HapII | CCGG | 1 cut(s) | 230 |
| HhaI | GCGC | 1 cut(s) | 236 |
| Hin1II | CATG | 2 cut(s) | 623, 721 |
| Hin6I | GCGC | 1 cut(s) | 234 |
| HinP1I | GCGC | 1 cut(s) | 234 |
| HinfI | GANTC | 6 cut(s) | 23, 56, 428, 434, 555, 631 |
| HpaII | CCGG | 1 cut(s) | 230 |
| HphI | GGTGA | 3 cut(s) | 56, 87, 306 |
| Hpy166II | GTNNAC | 1 cut(s) | 393 |
| Hpy188I | TCNGA | 2 cut(s) | 303, 433 |
| Hpy188III | TCNNGA | 1 cut(s) | 149 |
| Hpy8I | GTNNAC | 1 cut(s) | 393 |
| Hpy99I | CGWCG | 1 cut(s) | 650 |
| HpyCH4III | ACNGT | 2 cut(s) | 484, 702 |
| HpyCH4IV | ACGT | 2 cut(s) | 37, 339 |
| HpyCH4V | TGCA | 3 cut(s) | 125, 183, 209 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 119, 604 |
| HpyF3I | CTNAG | 3 cut(s) | 12, 387, 430 |
| HpySE526I | ACGT | 2 cut(s) | 37, 339 |
| Hsp92II | CATG | 2 cut(s) | 623, 721 |
| HspAI | GCGC | 1 cut(s) | 234 |
| Kzo9I | GATC | 1 cut(s) | 303 |
| Lsp1109I | GCAGC | 2 cut(s) | 109, 610 |
| LweI | GCATC | 2 cut(s) | 97, 166 |
| MaeII | ACGT | 2 cut(s) | 37, 339 |
| MaeIII | GTNAC | 2 cut(s) | 93, 237 |
| MalI | GATC | 1 cut(s) | 305 |
| MboI | GATC | 1 cut(s) | 303 |
| MboII | GAAGA | 4 cut(s) | 185, 284, 481, 662 |
| MfeI | CAATTG | 1 cut(s) | 352 |
| MluCI | AATT | 4 cut(s) | 158, 352, 669, 734 |
| MlyI | GAGTC | 3 cut(s) | 32, 50, 422 |
| MnlI | CCTC | 9 cut(s) | 75, 77, 78, 154, 237, 261, 372, 455, 531 |
| Mph1103I | ATGCAT | 1 cut(s) | 211 |
| MseI | TTAA | 2 cut(s) | 156, 714 |
| MslI | CAYNNNNRTG | 1 cut(s) | 103 |
| MspA1I | CMGCKG | 1 cut(s) | 590 |
| MspI | CCGG | 1 cut(s) | 230 |
| MspR9I | CCNGG | 2 cut(s) | 42, 231 |
| MunI | CAATTG | 1 cut(s) | 352 |
| Mva1269I | GAATGC | 1 cut(s) | 110 |
| MvaI | CCWGG | 1 cut(s) | 42 |
| MvnI | CGCG | 1 cut(s) | 236 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 119, 604 |
| NciI | CCSGG | 1 cut(s) | 231 |
| NdeI | CATATG | 1 cut(s) | 366 |
| NdeII | GATC | 1 cut(s) | 303 |
| NlaIII | CATG | 2 cut(s) | 623, 721 |
| NmuCI | GTSAC | 1 cut(s) | 93 |
| NsiI | ATGCAT | 1 cut(s) | 211 |
| NspI | RCATGY | 1 cut(s) | 721 |
| PctI | GAATGC | 1 cut(s) | 110 |
| PfeI | GAWTC | 3 cut(s) | 434, 555, 631 |
| PkrI | GCNGC | 2 cut(s) | 124, 600 |
| PleI | GAGTC | 3 cut(s) | 31, 50, 422 |
| PpsI | GAGTC | 3 cut(s) | 31, 50, 422 |
| Psp6I | CCWGG | 1 cut(s) | 40 |
| PspGI | CCWGG | 1 cut(s) | 40 |
| PvuII | CAGCTG | 1 cut(s) | 590 |
| RsaI | GTAC | 3 cut(s) | 486, 704, 746 |
| RsaNI | GTAC | 3 cut(s) | 485, 703, 745 |
| RseI | CAYNNNNRTG | 1 cut(s) | 103 |
| SaqAI | TTAA | 2 cut(s) | 156, 714 |
| SatI | GCNGC | 2 cut(s) | 123, 599 |
| Sau3AI | GATC | 1 cut(s) | 303 |
| ScaI | AGTACT | 1 cut(s) | 746 |
| SchI | GAGTC | 3 cut(s) | 32, 50, 422 |
| ScrFI | CCNGG | 2 cut(s) | 42, 231 |
| SfaNI | GCATC | 2 cut(s) | 97, 166 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 103 |
| Sse9I | AATT | 4 cut(s) | 158, 352, 669, 734 |
| SspI | AATATT | 1 cut(s) | 405 |
| StyD4I | CCNGG | 2 cut(s) | 40, 229 |
| TaaI | ACNGT | 2 cut(s) | 484, 702 |
| TaiI | ACGT | 2 cut(s) | 40, 342 |
| TaqII | GACCGA | 1 cut(s) | 96 |
| TasI | AATT | 4 cut(s) | 158, 352, 669, 734 |
| TatI | WGTACW | 3 cut(s) | 484, 702, 744 |
| TfiI | GAWTC | 3 cut(s) | 434, 555, 631 |
| Tru1I | TTAA | 2 cut(s) | 156, 714 |
| Tru9I | TTAA | 2 cut(s) | 156, 714 |
| TscAI | CASTG | 1 cut(s) | 570 |
| TseFI | GTSAC | 1 cut(s) | 93 |
| TseI | GCWGC | 2 cut(s) | 122, 598 |
| Tsp45I | GTSAC | 1 cut(s) | 93 |
| TspDTI | ATGAA | 1 cut(s) | 122 |
| TspRI | CASTG | 1 cut(s) | 570 |
| XceI | RCATGY | 1 cut(s) | 721 |
| ZrmI | AGTACT | 1 cut(s) | 746 |
| Zsp2I | ATGCAT | 1 cut(s) | 211 |