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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 564 |
| AccIII | TCCGGA | 1 cut(s) | 304 |
| AciI | CCGC | 3 cut(s) | 364, 449, 460 |
| AclWI | GGATC | 1 cut(s) | 49 |
| AcsI | RAATTY | 2 cut(s) | 25, 337 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 466 |
| AflIII | ACRYGT | 1 cut(s) | 567 |
| AgsI | TTSAA | 4 cut(s) | 100, 194, 244, 382 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 510 |
| AluBI | AGCT | 3 cut(s) | 18, 190, 431 |
| AluI | AGCT | 3 cut(s) | 18, 190, 431 |
| AlwI | GGATC | 1 cut(s) | 49 |
| Aor13HI | TCCGGA | 1 cut(s) | 304 |
| AoxI | GGCC | 1 cut(s) | 4 |
| ApeKI | GCWGC | 4 cut(s) | 18, 120, 144, 428 |
| ApoI | RAATTY | 2 cut(s) | 25, 337 |
| AspS9I | GGNCC | 3 cut(s) | 4, 512, 526 |
| AsuHPI | GGTGA | 3 cut(s) | 310, 370, 449 |
| AvaII | GGWCC | 2 cut(s) | 512, 526 |
| BanII | GRGCYC | 2 cut(s) | 407, 521 |
| BbvI | GCAGC | 4 cut(s) | 5, 131, 132, 440 |
| BccI | CCATC | 1 cut(s) | 501 |
| BceAI | ACGGC | 1 cut(s) | 457 |
| BisI | GCNGC | 4 cut(s) | 19, 121, 145, 429 |
| BlpI | GCTNAGC | 1 cut(s) | 432 |
| BlsI | GCNGC | 4 cut(s) | 20, 122, 146, 430 |
| Bme18I | GGWCC | 2 cut(s) | 512, 526 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 510 |
| BmgT120I | GGNCC | 3 cut(s) | 4, 512, 526 |
| BmiI | GGNNCC | 1 cut(s) | 445 |
| BmrI | ACTGGG | 1 cut(s) | 61 |
| BmsI | GCATC | 1 cut(s) | 359 |
| BmuI | ACTGGG | 1 cut(s) | 61 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 432 |
| BsaJI | CCNNGG | 2 cut(s) | 522, 537 |
| BsaWI | WCCGGW | 1 cut(s) | 304 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 466 |
| Bse1I | ACTGG | 1 cut(s) | 56 |
| BseAI | TCCGGA | 1 cut(s) | 304 |
| BseDI | CCNNGG | 2 cut(s) | 522, 537 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 466 |
| BseMII | CTCAG | 1 cut(s) | 446 |
| BseNI | ACTGG | 1 cut(s) | 56 |
| BseRI | GAGGAG | 2 cut(s) | 218, 221 |
| BseXI | GCAGC | 4 cut(s) | 5, 131, 132, 440 |
| BshFI | GGCC | 1 cut(s) | 6 |
| BsiSI | CCGG | 1 cut(s) | 305 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 466 |
| BsnI | GGCC | 1 cut(s) | 6 |
| Bsp1286I | GDGCHC | 2 cut(s) | 407, 521 |
| Bsp13I | TCCGGA | 1 cut(s) | 304 |
| Bsp143I | GATC | 2 cut(s) | 36, 41 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 432 |
| BspACI | CCGC | 3 cut(s) | 364, 449, 460 |
| BspANI | GGCC | 1 cut(s) | 6 |
| BspCNI | CTCAG | 1 cut(s) | 445 |
| BspEI | TCCGGA | 1 cut(s) | 304 |
| BspLI | GGNNCC | 1 cut(s) | 445 |
| BspPI | GGATC | 1 cut(s) | 49 |
| BsrI | ACTGG | 1 cut(s) | 56 |
| BssECI | CCNNGG | 2 cut(s) | 522, 537 |
| BssMI | GATC | 2 cut(s) | 36, 41 |
| Bst4CI | ACNGT | 4 cut(s) | 226, 512, 526, 541 |
| BstDEI | CTNAG | 2 cut(s) | 275, 432 |
| BstDSI | CCRYGG | 2 cut(s) | 522, 537 |
| BstKTI | GATC | 2 cut(s) | 39, 44 |
| BstMBI | GATC | 2 cut(s) | 36, 41 |
| BstNSI | RCATGY | 2 cut(s) | 535, 571 |
| BstV1I | GCAGC | 4 cut(s) | 5, 131, 132, 440 |
| BstX2I | RGATCY | 1 cut(s) | 41 |
| BstYI | RGATCY | 1 cut(s) | 41 |
| BsuRI | GGCC | 1 cut(s) | 6 |
| BtgI | CCRYGG | 2 cut(s) | 522, 537 |
| Cfr13I | GGNCC | 3 cut(s) | 4, 512, 526 |
| CpoI | CGGWCCG | 1 cut(s) | 512 |
| CspI | CGGWCCG | 1 cut(s) | 512 |
| CviAII | CATG | 3 cut(s) | 295, 532, 568 |
| CviJI | RGCY | 9 cut(s) | 6, 18, 123, 144, 190, 405, 431, 444, 519 |
| CviKI_1 | RGCY | 9 cut(s) | 6, 18, 123, 144, 190, 405, 431, 444, 519 |
| DdeI | CTNAG | 2 cut(s) | 275, 432 |
| DpnI | GATC | 2 cut(s) | 38, 43 |
| DpnII | GATC | 2 cut(s) | 36, 41 |
| DraI | TTTAAA | 1 cut(s) | 79 |
| DriI | GACNNNNNGTC | 1 cut(s) | 510 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 510 |
| Eco24I | GRGCYC | 2 cut(s) | 407, 521 |
| Eco47I | GGWCC | 2 cut(s) | 512, 526 |
| EcoT38I | GRGCYC | 2 cut(s) | 407, 521 |
| FaeI | CATG | 3 cut(s) | 298, 535, 571 |
| FatI | CATG | 3 cut(s) | 294, 531, 567 |
| FauI | CCCGC | 1 cut(s) | 456 |
| FauNDI | CATATG | 1 cut(s) | 127 |
| FblI | GTMKAC | 1 cut(s) | 564 |
| Fnu4HI | GCNGC | 4 cut(s) | 19, 121, 145, 429 |
| FriOI | GRGCYC | 2 cut(s) | 407, 521 |
| Fsp4HI | GCNGC | 4 cut(s) | 19, 121, 145, 429 |
| GluI | GCNGC | 4 cut(s) | 19, 121, 145, 429 |
| HaeIII | GGCC | 1 cut(s) | 6 |
| HapII | CCGG | 1 cut(s) | 305 |
| Hin1II | CATG | 3 cut(s) | 298, 535, 571 |
| HincII | GTYRAC | 1 cut(s) | 565 |
| HindII | GTYRAC | 1 cut(s) | 565 |
| HinfI | GANTC | 4 cut(s) | 113, 301, 412, 505 |
| HpaII | CCGG | 1 cut(s) | 305 |
| HphI | GGTGA | 3 cut(s) | 310, 370, 449 |
| Hpy166II | GTNNAC | 2 cut(s) | 529, 565 |
| Hpy188I | TCNGA | 2 cut(s) | 41, 516 |
| Hpy188III | TCNNGA | 1 cut(s) | 305 |
| Hpy8I | GTNNAC | 2 cut(s) | 529, 565 |
| HpyAV | CCTTC | 1 cut(s) | 376 |
| HpyCH4III | ACNGT | 4 cut(s) | 226, 512, 526, 541 |
| HpyCH4IV | ACGT | 2 cut(s) | 10, 160 |
| HpyCH4V | TGCA | 1 cut(s) | 350 |
| HpyF3I | CTNAG | 2 cut(s) | 275, 432 |
| HpySE526I | ACGT | 2 cut(s) | 10, 160 |
| Hsp92II | CATG | 3 cut(s) | 298, 535, 571 |
| Kpn2I | TCCGGA | 1 cut(s) | 304 |
| Kzo9I | GATC | 2 cut(s) | 36, 41 |
| LmnI | GCTCC | 1 cut(s) | 449 |
| LpnPI | CCDG | 3 cut(s) | 37, 254, 318 |
| Lsp1109I | GCAGC | 4 cut(s) | 5, 131, 132, 440 |
| LweI | GCATC | 1 cut(s) | 359 |
| MaeII | ACGT | 2 cut(s) | 10, 160 |
| MalI | GATC | 2 cut(s) | 38, 43 |
| MboI | GATC | 2 cut(s) | 36, 41 |
| MboII | GAAGA | 2 cut(s) | 256, 484 |
| MflI | RGATCY | 1 cut(s) | 41 |
| MhlI | GDGCHC | 2 cut(s) | 407, 521 |
| MluCI | AATT | 5 cut(s) | 25, 238, 337, 377, 386 |
| MlyI | GAGTC | 3 cut(s) | 122, 406, 499 |
| MmeI | TCCRAC | 1 cut(s) | 46 |
| MnlI | CCTC | 9 cut(s) | 51, 196, 199, 283, 301, 364, 430, 465, 538 |
| MroI | TCCGGA | 1 cut(s) | 304 |
| MseI | TTAA | 1 cut(s) | 78 |
| MspI | CCGG | 1 cut(s) | 305 |
| NdeI | CATATG | 1 cut(s) | 127 |
| NdeII | GATC | 2 cut(s) | 36, 41 |
| NlaIII | CATG | 3 cut(s) | 298, 535, 571 |
| NlaIV | GGNNCC | 1 cut(s) | 445 |
| NspI | RCATGY | 2 cut(s) | 535, 571 |
| PciI | ACATGT | 1 cut(s) | 567 |
| PfeI | GAWTC | 1 cut(s) | 301 |
| PkrI | GCNGC | 4 cut(s) | 20, 122, 146, 430 |
| PleI | GAGTC | 3 cut(s) | 121, 406, 499 |
| PpsI | GAGTC | 3 cut(s) | 121, 406, 499 |
| PscI | ACATGT | 1 cut(s) | 567 |
| PspN4I | GGNNCC | 1 cut(s) | 445 |
| PspPI | GGNCC | 3 cut(s) | 4, 512, 526 |
| PsuI | RGATCY | 1 cut(s) | 41 |
| Rsr2I | CGGWCCG | 1 cut(s) | 512 |
| RsrII | CGGWCCG | 1 cut(s) | 512 |
| SalI | GTCGAC | 1 cut(s) | 563 |
| SaqAI | TTAA | 1 cut(s) | 78 |
| SatI | GCNGC | 4 cut(s) | 19, 121, 145, 429 |
| Sau3AI | GATC | 2 cut(s) | 36, 41 |
| Sau96I | GGNCC | 3 cut(s) | 4, 512, 526 |
| SchI | GAGTC | 3 cut(s) | 122, 406, 499 |
| SduI | GDGCHC | 2 cut(s) | 407, 521 |
| SetI | ASST | 8 cut(s) | 13, 20, 163, 192, 375, 422, 433, 457 |
| SfaNI | GCATC | 1 cut(s) | 359 |
| SinI | GGWCC | 2 cut(s) | 512, 526 |
| Sse9I | AATT | 5 cut(s) | 25, 238, 337, 377, 386 |
| SsiI | CCGC | 3 cut(s) | 364, 449, 460 |
| SspI | AATATT | 1 cut(s) | 399 |
| TaaI | ACNGT | 4 cut(s) | 226, 512, 526, 541 |
| TaiI | ACGT | 2 cut(s) | 13, 163 |
| TaqI | TCGA | 2 cut(s) | 564, 574 |
| TaqII | GACCGA | 1 cut(s) | 347 |
| TasI | AATT | 5 cut(s) | 25, 238, 337, 377, 386 |
| TfiI | GAWTC | 1 cut(s) | 301 |
| Tru1I | TTAA | 1 cut(s) | 78 |
| Tru9I | TTAA | 1 cut(s) | 78 |
| TseI | GCWGC | 4 cut(s) | 18, 120, 144, 428 |
| VpaK11BI | GGWCC | 2 cut(s) | 512, 526 |
| XapI | RAATTY | 2 cut(s) | 25, 337 |
| XceI | RCATGY | 2 cut(s) | 535, 571 |
| XmiI | GTMKAC | 1 cut(s) | 564 |