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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 2 cut(s) | 257, 352 |
| AcoI | YGGCCR | 1 cut(s) | 236 |
| AfaI | GTAC | 2 cut(s) | 158, 200 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 257, 352 |
| AgsI | TTSAA | 2 cut(s) | 128, 304 |
| AluBI | AGCT | 4 cut(s) | 44, 163, 222, 275 |
| AluI | AGCT | 4 cut(s) | 44, 163, 222, 275 |
| AoxI | GGCC | 3 cut(s) | 236, 277, 319 |
| ApeKI | GCWGC | 1 cut(s) | 52 |
| AseI | ATTAAT | 1 cut(s) | 87 |
| BalI | TGGCCA | 1 cut(s) | 238 |
| BbvI | GCAGC | 1 cut(s) | 64 |
| BfaI | CTAG | 3 cut(s) | 228, 281, 323 |
| BisI | GCNGC | 1 cut(s) | 53 |
| BlsI | GCNGC | 1 cut(s) | 54 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 257, 352 |
| Bse118I | RCCGGY | 1 cut(s) | 317 |
| Bse1I | ACTGG | 3 cut(s) | 24, 206, 239 |
| BseGI | GGATG | 1 cut(s) | 100 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 257, 352 |
| BseNI | ACTGG | 3 cut(s) | 24, 206, 239 |
| BseXI | GCAGC | 1 cut(s) | 64 |
| BshFI | GGCC | 3 cut(s) | 238, 279, 321 |
| BsiSI | CCGG | 1 cut(s) | 318 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 257, 352 |
| BsnI | GGCC | 3 cut(s) | 238, 279, 321 |
| Bsp1407I | TGTACA | 1 cut(s) | 156 |
| Bsp143I | GATC | 3 cut(s) | 253, 294, 348 |
| BspANI | GGCC | 3 cut(s) | 238, 279, 321 |
| BsrFI | RCCGGY | 1 cut(s) | 317 |
| BsrGI | TGTACA | 1 cut(s) | 156 |
| BsrI | ACTGG | 3 cut(s) | 24, 206, 239 |
| BssAI | RCCGGY | 1 cut(s) | 317 |
| BssMI | GATC | 3 cut(s) | 253, 294, 348 |
| Bst4CI | ACNGT | 4 cut(s) | 156, 248, 289, 343 |
| BstAUI | TGTACA | 1 cut(s) | 156 |
| BstC8I | GCNNGC | 2 cut(s) | 277, 319 |
| BstF5I | GGATG | 1 cut(s) | 100 |
| BstKTI | GATC | 3 cut(s) | 256, 297, 351 |
| BstMBI | GATC | 3 cut(s) | 253, 294, 348 |
| BstV1I | GCAGC | 1 cut(s) | 64 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 275 |
| BsuRI | GGCC | 3 cut(s) | 238, 279, 321 |
| BtsCI | GGATG | 1 cut(s) | 100 |
| BtsIMutI | CAGTG | 5 cut(s) | 17, 232, 244, 285, 339 |
| Cac8I | GCNNGC | 2 cut(s) | 277, 319 |
| Cfr10I | RCCGGY | 1 cut(s) | 317 |
| Csp6I | GTAC | 2 cut(s) | 157, 199 |
| CviAII | CATG | 6 cut(s) | 74, 257, 269, 311, 352, 364 |
| CviQI | GTAC | 2 cut(s) | 157, 199 |
| DpnI | GATC | 3 cut(s) | 255, 296, 350 |
| DpnII | GATC | 3 cut(s) | 253, 294, 348 |
| EaeI | YGGCCR | 1 cut(s) | 236 |
| FaeI | CATG | 6 cut(s) | 77, 260, 272, 314, 355, 367 |
| FaiI | YATR | 6 cut(s) | 75, 258, 270, 312, 353, 365 |
| FatI | CATG | 6 cut(s) | 73, 256, 268, 310, 351, 363 |
| Fnu4HI | GCNGC | 1 cut(s) | 53 |
| FokI | GGATG | 1 cut(s) | 107 |
| Fsp4HI | GCNGC | 1 cut(s) | 53 |
| FspBI | CTAG | 3 cut(s) | 228, 281, 323 |
| GluI | GCNGC | 1 cut(s) | 53 |
| HaeIII | GGCC | 3 cut(s) | 238, 279, 321 |
| HapII | CCGG | 1 cut(s) | 318 |
| Hin1II | CATG | 6 cut(s) | 77, 260, 272, 314, 355, 367 |
| HinfI | GANTC | 1 cut(s) | 9 |
| HpaII | CCGG | 1 cut(s) | 318 |
| Hpy188III | TCNNGA | 3 cut(s) | 251, 292, 346 |
| HpyCH4III | ACNGT | 4 cut(s) | 156, 248, 289, 343 |
| Hsp92II | CATG | 6 cut(s) | 77, 260, 272, 314, 355, 367 |
| KroI | GCCGGC | 1 cut(s) | 317 |
| KroNI | GCCGGC | 1 cut(s) | 319 |
| Kzo9I | GATC | 3 cut(s) | 253, 294, 348 |
| Lsp1109I | GCAGC | 1 cut(s) | 64 |
| MaeI | CTAG | 3 cut(s) | 228, 281, 323 |
| MaeIII | GTNAC | 4 cut(s) | 230, 242, 283, 337 |
| MalI | GATC | 3 cut(s) | 255, 296, 350 |
| MboI | GATC | 3 cut(s) | 253, 294, 348 |
| MfeI | CAATTG | 2 cut(s) | 262, 357 |
| MlsI | TGGCCA | 1 cut(s) | 238 |
| MluCI | AATT | 4 cut(s) | 123, 188, 262, 357 |
| MluNI | TGGCCA | 1 cut(s) | 238 |
| MnlI | CCTC | 2 cut(s) | 55, 94 |
| Mox20I | TGGCCA | 1 cut(s) | 238 |
| MroNI | GCCGGC | 1 cut(s) | 317 |
| MscI | TGGCCA | 1 cut(s) | 238 |
| MseI | TTAA | 1 cut(s) | 87 |
| Msp20I | TGGCCA | 1 cut(s) | 238 |
| MspA1I | CMGCKG | 1 cut(s) | 44 |
| MspI | CCGG | 1 cut(s) | 318 |
| MunI | CAATTG | 2 cut(s) | 262, 357 |
| NaeI | GCCGGC | 1 cut(s) | 319 |
| NdeII | GATC | 3 cut(s) | 253, 294, 348 |
| NgoMIV | GCCGGC | 1 cut(s) | 317 |
| NlaIII | CATG | 6 cut(s) | 77, 260, 272, 314, 355, 367 |
| NmuCI | GTSAC | 4 cut(s) | 230, 242, 283, 337 |
| PdiI | GCCGGC | 1 cut(s) | 319 |
| PfeI | GAWTC | 1 cut(s) | 9 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 257, 352 |
| PkrI | GCNGC | 1 cut(s) | 54 |
| PshBI | ATTAAT | 1 cut(s) | 87 |
| PvuII | CAGCTG | 1 cut(s) | 44 |
| RsaI | GTAC | 2 cut(s) | 158, 200 |
| RsaNI | GTAC | 2 cut(s) | 157, 199 |
| SaqAI | TTAA | 1 cut(s) | 87 |
| SatI | GCNGC | 1 cut(s) | 53 |
| Sau3AI | GATC | 3 cut(s) | 253, 294, 348 |
| SetI | ASST | 4 cut(s) | 46, 165, 224, 277 |
| Sse9I | AATT | 4 cut(s) | 123, 188, 262, 357 |
| SspMI | CTAG | 3 cut(s) | 228, 281, 323 |
| TaaI | ACNGT | 4 cut(s) | 156, 248, 289, 343 |
| TasI | AATT | 4 cut(s) | 123, 188, 262, 357 |
| TatI | WGTACW | 2 cut(s) | 156, 198 |
| TfiI | GAWTC | 1 cut(s) | 9 |
| Tru1I | TTAA | 1 cut(s) | 87 |
| Tru9I | TTAA | 1 cut(s) | 87 |
| TscAI | CASTG | 5 cut(s) | 24, 239, 251, 292, 346 |
| TseFI | GTSAC | 4 cut(s) | 230, 242, 283, 337 |
| TseI | GCWGC | 1 cut(s) | 52 |
| Tsp45I | GTSAC | 4 cut(s) | 230, 242, 283, 337 |
| TspRI | CASTG | 5 cut(s) | 24, 239, 251, 292, 346 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 257, 352 |
| VspI | ATTAAT | 1 cut(s) | 87 |
| XspI | CTAG | 3 cut(s) | 228, 281, 323 |