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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 361 |
| AgsI | TTSAA | 1 cut(s) | 264 |
| AjiI | CACGTC | 1 cut(s) | 55 |
| AluBI | AGCT | 2 cut(s) | 211, 320 |
| AluI | AGCT | 2 cut(s) | 211, 320 |
| ApeKI | GCWGC | 3 cut(s) | 141, 211, 214 |
| ApoI | RAATTY | 1 cut(s) | 361 |
| AspS9I | GGNCC | 1 cut(s) | 337 |
| AsuII | TTCGAA | 1 cut(s) | 365 |
| AvaII | GGWCC | 1 cut(s) | 337 |
| BbsI | GAAGAC | 1 cut(s) | 20 |
| BbvI | GCAGC | 3 cut(s) | 128, 198, 201 |
| BceAI | ACGGC | 2 cut(s) | 252, 343 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 270, 304 |
| BfmI | CTRYAG | 1 cut(s) | 215 |
| BisI | GCNGC | 3 cut(s) | 142, 212, 215 |
| BlsI | GCNGC | 3 cut(s) | 143, 213, 216 |
| Bme18I | GGWCC | 1 cut(s) | 337 |
| BmgBI | CACGTC | 1 cut(s) | 55 |
| BmgT120I | GGNCC | 1 cut(s) | 337 |
| BmsI | GCATC | 2 cut(s) | 235, 268 |
| BpiI | GAAGAC | 1 cut(s) | 20 |
| Bpu14I | TTCGAA | 1 cut(s) | 365 |
| BsaJI | CCNNGG | 1 cut(s) | 253 |
| BseDI | CCNNGG | 1 cut(s) | 253 |
| BseGI | GGATG | 2 cut(s) | 283, 298 |
| BseXI | GCAGC | 3 cut(s) | 128, 198, 201 |
| BsgI | GTGCAG | 2 cut(s) | 127, 152 |
| BslFI | GGGAC | 2 cut(s) | 77, 214 |
| BsmFI | GGGAC | 2 cut(s) | 77, 214 |
| BsmI | GAATGC | 1 cut(s) | 248 |
| Bsp119I | TTCGAA | 1 cut(s) | 365 |
| Bsp19I | CCATGG | 1 cut(s) | 253 |
| BspMAI | CTGCAG | 1 cut(s) | 219 |
| BspT104I | TTCGAA | 1 cut(s) | 365 |
| BssECI | CCNNGG | 1 cut(s) | 253 |
| BssT1I | CCWWGG | 1 cut(s) | 253 |
| Bst6I | CTCTTC | 1 cut(s) | 75 |
| BstBI | TTCGAA | 1 cut(s) | 365 |
| BstC8I | GCNNGC | 1 cut(s) | 153 |
| BstDSI | CCRYGG | 1 cut(s) | 253 |
| BstF5I | GGATG | 2 cut(s) | 283, 298 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 245 |
| BstSFI | CTRYAG | 1 cut(s) | 215 |
| BstV1I | GCAGC | 3 cut(s) | 128, 198, 201 |
| BstV2I | GAAGAC | 1 cut(s) | 20 |
| BtgI | CCRYGG | 1 cut(s) | 253 |
| BtrI | CACGTC | 1 cut(s) | 55 |
| BtsCI | GGATG | 2 cut(s) | 283, 298 |
| Cac8I | GCNNGC | 1 cut(s) | 153 |
| Cfr13I | GGNCC | 1 cut(s) | 337 |
| CviAII | CATG | 1 cut(s) | 254 |
| CviJI | RGCY | 2 cut(s) | 211, 320 |
| CviKI_1 | RGCY | 2 cut(s) | 211, 320 |
| Eam1104I | CTCTTC | 1 cut(s) | 75 |
| EarI | CTCTTC | 1 cut(s) | 75 |
| Eco130I | CCWWGG | 1 cut(s) | 253 |
| Eco47I | GGWCC | 1 cut(s) | 337 |
| EcoT14I | CCWWGG | 1 cut(s) | 253 |
| EcoT22I | ATGCAT | 1 cut(s) | 250 |
| ErhI | CCWWGG | 1 cut(s) | 253 |
| FaeI | CATG | 1 cut(s) | 257 |
| FaiI | YATR | 3 cut(s) | 114, 129, 255 |
| FaqI | GGGAC | 2 cut(s) | 77, 214 |
| FatI | CATG | 1 cut(s) | 253 |
| Fnu4HI | GCNGC | 3 cut(s) | 142, 212, 215 |
| FokI | GGATG | 2 cut(s) | 290, 305 |
| Fsp4HI | GCNGC | 3 cut(s) | 142, 212, 215 |
| GluI | GCNGC | 3 cut(s) | 142, 212, 215 |
| Hin1II | CATG | 1 cut(s) | 257 |
| HincII | GTYRAC | 1 cut(s) | 373 |
| HindII | GTYRAC | 1 cut(s) | 373 |
| HindIII | AAGCTT | 1 cut(s) | 318 |
| Hpy166II | GTNNAC | 1 cut(s) | 373 |
| Hpy188I | TCNGA | 2 cut(s) | 162, 292 |
| Hpy8I | GTNNAC | 1 cut(s) | 373 |
| Hpy99I | CGWCG | 2 cut(s) | 56, 329 |
| HpyAV | CCTTC | 2 cut(s) | 34, 191 |
| HpyCH4IV | ACGT | 1 cut(s) | 54 |
| HpyCH4V | TGCA | 8 cut(s) | 32, 133, 144, 173, 217, 233, 248, 281 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 245 |
| HpySE526I | ACGT | 1 cut(s) | 54 |
| Hsp92II | CATG | 1 cut(s) | 257 |
| Lsp1109I | GCAGC | 3 cut(s) | 128, 198, 201 |
| LweI | GCATC | 2 cut(s) | 235, 268 |
| MaeII | ACGT | 1 cut(s) | 54 |
| MboII | GAAGA | 6 cut(s) | 25, 49, 56, 71, 74, 92 |
| MfeI | CAATTG | 1 cut(s) | 168 |
| MluCI | AATT | 3 cut(s) | 168, 264, 361 |
| MnlI | CCTC | 4 cut(s) | 76, 115, 200, 251 |
| Mph1103I | ATGCAT | 1 cut(s) | 250 |
| MseI | TTAA | 2 cut(s) | 267, 299 |
| MunI | CAATTG | 1 cut(s) | 168 |
| Mva1269I | GAATGC | 1 cut(s) | 248 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 245 |
| NcoI | CCATGG | 1 cut(s) | 253 |
| NlaIII | CATG | 1 cut(s) | 257 |
| NsiI | ATGCAT | 1 cut(s) | 250 |
| NspV | TTCGAA | 1 cut(s) | 365 |
| PctI | GAATGC | 1 cut(s) | 248 |
| PkrI | GCNGC | 3 cut(s) | 143, 213, 216 |
| PspPI | GGNCC | 1 cut(s) | 337 |
| PstI | CTGCAG | 1 cut(s) | 219 |
| SaqAI | TTAA | 2 cut(s) | 267, 299 |
| SatI | GCNGC | 3 cut(s) | 142, 212, 215 |
| Sau96I | GGNCC | 1 cut(s) | 337 |
| SetI | ASST | 6 cut(s) | 57, 192, 213, 262, 322, 342 |
| SfaNI | GCATC | 2 cut(s) | 235, 268 |
| SfcI | CTRYAG | 1 cut(s) | 215 |
| SfuI | TTCGAA | 1 cut(s) | 365 |
| SgeI | CNNG | 6 cut(s) | 67, 164, 203, 266, 329, 346 |
| SinI | GGWCC | 1 cut(s) | 337 |
| Sse9I | AATT | 3 cut(s) | 168, 264, 361 |
| StyI | CCWWGG | 1 cut(s) | 253 |
| TaiI | ACGT | 1 cut(s) | 57 |
| TaqI | TCGA | 2 cut(s) | 192, 365 |
| TasI | AATT | 3 cut(s) | 168, 264, 361 |
| Tru1I | TTAA | 2 cut(s) | 267, 299 |
| Tru9I | TTAA | 2 cut(s) | 267, 299 |
| TseI | GCWGC | 3 cut(s) | 141, 211, 214 |
| TspDTI | ATGAA | 2 cut(s) | 93, 210 |
| TspGWI | ACGGA | 1 cut(s) | 350 |
| VpaK11BI | GGWCC | 1 cut(s) | 337 |
| XapI | RAATTY | 1 cut(s) | 361 |
| Zsp2I | ATGCAT | 1 cut(s) | 250 |