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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 2 cut(s) | 459, 588 |
| AgsI | TTSAA | 3 cut(s) | 64, 173, 563 |
| AjiI | CACGTC | 1 cut(s) | 329 |
| AjnI | CCWGG | 1 cut(s) | 331 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 78, 110 |
| AluBI | AGCT | 2 cut(s) | 31, 581 |
| AluI | AGCT | 2 cut(s) | 31, 581 |
| Alw26I | GTCTC | 1 cut(s) | 321 |
| Ama87I | CYCGRG | 2 cut(s) | 402, 608 |
| AoxI | GGCC | 1 cut(s) | 396 |
| ApeKI | GCWGC | 1 cut(s) | 581 |
| ApoI | RAATTY | 2 cut(s) | 459, 588 |
| AspLEI | GCGC | 1 cut(s) | 503 |
| AspS9I | GGNCC | 1 cut(s) | 397 |
| AsuHPI | GGTGA | 2 cut(s) | 546, 588 |
| AvaI | CYCGRG | 2 cut(s) | 402, 608 |
| BbvI | GCAGC | 1 cut(s) | 568 |
| BccI | CCATC | 2 cut(s) | 109, 286 |
| BceAI | ACGGC | 1 cut(s) | 510 |
| BciT130I | CCWGG | 1 cut(s) | 333 |
| BcoDI | GTCTC | 1 cut(s) | 321 |
| BfaI | CTAG | 1 cut(s) | 372 |
| BfmI | CTRYAG | 1 cut(s) | 519 |
| BisI | GCNGC | 1 cut(s) | 582 |
| BlsI | GCNGC | 1 cut(s) | 583 |
| Bme1390I | CCNGG | 1 cut(s) | 333 |
| BmeT110I | CYCGRG | 2 cut(s) | 402, 608 |
| BmgBI | CACGTC | 1 cut(s) | 329 |
| BmgT120I | GGNCC | 1 cut(s) | 397 |
| BmiI | GGNNCC | 1 cut(s) | 399 |
| BmrFI | CCNGG | 1 cut(s) | 333 |
| BmsI | GCATC | 2 cut(s) | 512, 624 |
| BoxI | GACNNNNGTC | 1 cut(s) | 550 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 146, 178, 236, 268 |
| BpmI | CTGGAG | 1 cut(s) | 53 |
| BpuEI | CTTGAG | 1 cut(s) | 435 |
| BsaJI | CCNNGG | 4 cut(s) | 401, 492, 541, 609 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 110, 140, 343, 373 |
| BseBI | CCWGG | 1 cut(s) | 333 |
| BseDI | CCNNGG | 4 cut(s) | 401, 492, 541, 609 |
| BseGI | GGATG | 3 cut(s) | 127, 214, 503 |
| BseRI | GAGGAG | 2 cut(s) | 345, 348 |
| BseXI | GCAGC | 1 cut(s) | 568 |
| BsgI | GTGCAG | 1 cut(s) | 567 |
| BshFI | GGCC | 1 cut(s) | 398 |
| BsiHKCI | CYCGRG | 2 cut(s) | 402, 608 |
| BsmAI | GTCTC | 1 cut(s) | 321 |
| BsmI | GAATGC | 1 cut(s) | 569 |
| BsnI | GGCC | 1 cut(s) | 398 |
| BsoBI | CYCGRG | 2 cut(s) | 402, 608 |
| BspANI | GGCC | 1 cut(s) | 398 |
| BspHI | TCATGA | 1 cut(s) | 337 |
| BspLI | GGNNCC | 1 cut(s) | 399 |
| BspMAI | CTGCAG | 1 cut(s) | 523 |
| BssECI | CCNNGG | 4 cut(s) | 401, 492, 541, 609 |
| Bst2UI | CCWGG | 1 cut(s) | 333 |
| Bst6I | CTCTTC | 3 cut(s) | 42, 105, 195 |
| BstC8I | GCNNGC | 3 cut(s) | 428, 499, 639 |
| BstDSI | CCRYGG | 1 cut(s) | 492 |
| BstEII | GGTNACC | 1 cut(s) | 552 |
| BstF5I | GGATG | 3 cut(s) | 127, 214, 503 |
| BstHHI | GCGC | 1 cut(s) | 503 |
| BstMAI | GTCTC | 1 cut(s) | 321 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 578 |
| BstNI | CCWGG | 1 cut(s) | 333 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 550 |
| BstPI | GGTNACC | 1 cut(s) | 552 |
| BstSCI | CCNGG | 1 cut(s) | 331 |
| BstSFI | CTRYAG | 1 cut(s) | 519 |
| BstV1I | GCAGC | 1 cut(s) | 568 |
| BsuRI | GGCC | 1 cut(s) | 398 |
| BtgI | CCRYGG | 1 cut(s) | 492 |
| BtrI | CACGTC | 1 cut(s) | 329 |
| BtsCI | GGATG | 3 cut(s) | 127, 214, 503 |
| BtsIMutI | CAGTG | 1 cut(s) | 254 |
| Cac8I | GCNNGC | 3 cut(s) | 428, 499, 639 |
| CciI | TCATGA | 1 cut(s) | 337 |
| CfoI | GCGC | 1 cut(s) | 503 |
| Cfr13I | GGNCC | 1 cut(s) | 397 |
| CviAII | CATG | 1 cut(s) | 338 |
| CviJI | RGCY | 6 cut(s) | 31, 161, 251, 398, 413, 581 |
| CviKI_1 | RGCY | 6 cut(s) | 31, 161, 251, 398, 413, 581 |
| Eam1104I | CTCTTC | 3 cut(s) | 42, 105, 195 |
| EarI | CTCTTC | 3 cut(s) | 42, 105, 195 |
| Eco88I | CYCGRG | 2 cut(s) | 402, 608 |
| Eco91I | GGTNACC | 1 cut(s) | 552 |
| EcoO109I | RGGNCCY | 1 cut(s) | 397 |
| EcoO65I | GGTNACC | 1 cut(s) | 552 |
| EcoRI | GAATTC | 1 cut(s) | 459 |
| EcoRII | CCWGG | 1 cut(s) | 331 |
| FaeI | CATG | 1 cut(s) | 341 |
| FaiI | YATR | 3 cut(s) | 287, 312, 339 |
| FatI | CATG | 1 cut(s) | 337 |
| Fnu4HI | GCNGC | 1 cut(s) | 582 |
| FokI | GGATG | 3 cut(s) | 134, 221, 490 |
| Fsp4HI | GCNGC | 1 cut(s) | 582 |
| FspBI | CTAG | 1 cut(s) | 372 |
| GlaI | GCGC | 1 cut(s) | 502 |
| GluI | GCNGC | 1 cut(s) | 582 |
| GsuI | CTGGAG | 1 cut(s) | 53 |
| HaeIII | GGCC | 1 cut(s) | 398 |
| HhaI | GCGC | 1 cut(s) | 503 |
| Hin1II | CATG | 1 cut(s) | 341 |
| Hin6I | GCGC | 1 cut(s) | 501 |
| HinP1I | GCGC | 1 cut(s) | 501 |
| HinfI | GANTC | 2 cut(s) | 314, 530 |
| HphI | GGTGA | 2 cut(s) | 546, 588 |
| Hpy188I | TCNGA | 2 cut(s) | 465, 508 |
| Hpy188III | TCNNGA | 3 cut(s) | 77, 338, 608 |
| HpyAV | CCTTC | 1 cut(s) | 199 |
| HpyCH4IV | ACGT | 1 cut(s) | 328 |
| HpyCH4V | TGCA | 3 cut(s) | 521, 584, 641 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 578 |
| HpySE526I | ACGT | 1 cut(s) | 328 |
| Hsp92II | CATG | 1 cut(s) | 341 |
| HspAI | GCGC | 1 cut(s) | 501 |
| LpnPI | CCDG | 3 cut(s) | 17, 318, 345 |
| Lsp1109I | GCAGC | 1 cut(s) | 568 |
| LweI | GCATC | 2 cut(s) | 512, 624 |
| MaeI | CTAG | 1 cut(s) | 372 |
| MaeII | ACGT | 1 cut(s) | 328 |
| MaeIII | GTNAC | 1 cut(s) | 552 |
| MboII | GAAGA | 7 cut(s) | 16, 21, 56, 59, 122, 212, 643 |
| MfeI | CAATTG | 1 cut(s) | 275 |
| MluCI | AATT | 8 cut(s) | 16, 93, 150, 183, 275, 459, 588, 617 |
| MlyI | GAGTC | 2 cut(s) | 323, 524 |
| MmeI | TCCRAC | 1 cut(s) | 436 |
| MseI | TTAA | 5 cut(s) | 218, 261, 299, 615, 661 |
| MslI | CAYNNNNRTG | 2 cut(s) | 290, 562 |
| MspR9I | CCNGG | 1 cut(s) | 333 |
| MunI | CAATTG | 1 cut(s) | 275 |
| Mva1269I | GAATGC | 1 cut(s) | 569 |
| MvaI | CCWGG | 1 cut(s) | 333 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 578 |
| NlaIII | CATG | 1 cut(s) | 341 |
| NlaIV | GGNNCC | 1 cut(s) | 399 |
| NmuCI | GTSAC | 1 cut(s) | 552 |
| PagI | TCATGA | 1 cut(s) | 337 |
| PctI | GAATGC | 1 cut(s) | 569 |
| PkrI | GCNGC | 1 cut(s) | 583 |
| PleI | GAGTC | 2 cut(s) | 322, 524 |
| PpsI | GAGTC | 2 cut(s) | 322, 524 |
| PshAI | GACNNNNGTC | 1 cut(s) | 550 |
| Psp6I | CCWGG | 1 cut(s) | 331 |
| PspEI | GGTNACC | 1 cut(s) | 552 |
| PspGI | CCWGG | 1 cut(s) | 331 |
| PspN4I | GGNNCC | 1 cut(s) | 399 |
| PspPI | GGNCC | 1 cut(s) | 397 |
| PstI | CTGCAG | 1 cut(s) | 523 |
| RseI | CAYNNNNRTG | 2 cut(s) | 290, 562 |
| SaqAI | TTAA | 5 cut(s) | 218, 261, 299, 615, 661 |
| SatI | GCNGC | 1 cut(s) | 582 |
| Sau96I | GGNCC | 1 cut(s) | 397 |
| SchI | GAGTC | 2 cut(s) | 323, 524 |
| ScrFI | CCNGG | 1 cut(s) | 333 |
| SfaNI | GCATC | 2 cut(s) | 512, 624 |
| SfcI | CTRYAG | 1 cut(s) | 519 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 290, 562 |
| SmlI | CTYRAG | 1 cut(s) | 450 |
| SmoI | CTYRAG | 1 cut(s) | 450 |
| Sse9I | AATT | 8 cut(s) | 16, 93, 150, 183, 275, 459, 588, 617 |
| SspMI | CTAG | 1 cut(s) | 372 |
| StyD4I | CCNGG | 1 cut(s) | 331 |
| TaiI | ACGT | 1 cut(s) | 331 |
| TaqII | GACCGA | 1 cut(s) | 540 |
| TasI | AATT | 8 cut(s) | 16, 93, 150, 183, 275, 459, 588, 617 |
| Tru1I | TTAA | 5 cut(s) | 218, 261, 299, 615, 661 |
| Tru9I | TTAA | 5 cut(s) | 218, 261, 299, 615, 661 |
| TscAI | CASTG | 1 cut(s) | 261 |
| TseFI | GTSAC | 1 cut(s) | 552 |
| TseI | GCWGC | 1 cut(s) | 581 |
| Tsp45I | GTSAC | 1 cut(s) | 552 |
| TspDTI | ATGAA | 3 cut(s) | 17, 29, 581 |
| TspGWI | ACGGA | 1 cut(s) | 209 |
| TspRI | CASTG | 1 cut(s) | 261 |
| XapI | RAATTY | 2 cut(s) | 459, 588 |
| XspI | CTAG | 1 cut(s) | 372 |