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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 549 |
| AccB1I | GGYRCC | 1 cut(s) | 549 |
| AciI | CCGC | 3 cut(s) | 17, 532, 539 |
| AclWI | GGATC | 1 cut(s) | 585 |
| AcsI | RAATTY | 1 cut(s) | 488 |
| AcuI | CTGAAG | 1 cut(s) | 108 |
| AfaI | GTAC | 2 cut(s) | 157, 551 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 566 |
| AgsI | TTSAA | 2 cut(s) | 323, 390 |
| AluBI | AGCT | 3 cut(s) | 86, 337, 446 |
| AluI | AGCT | 3 cut(s) | 86, 337, 446 |
| AlwI | GGATC | 1 cut(s) | 585 |
| ApeKI | GCWGC | 3 cut(s) | 266, 337, 340 |
| ApoI | RAATTY | 1 cut(s) | 488 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 102 |
| Asp718I | GGTACC | 1 cut(s) | 549 |
| BanI | GGYRCC | 1 cut(s) | 549 |
| BbsI | GAAGAC | 1 cut(s) | 146 |
| BbvI | GCAGC | 3 cut(s) | 253, 324, 327 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 396, 430, 560, 594 |
| BfmI | CTRYAG | 1 cut(s) | 341 |
| BisI | GCNGC | 3 cut(s) | 267, 338, 341 |
| BlsI | GCNGC | 3 cut(s) | 268, 339, 342 |
| BmiI | GGNNCC | 1 cut(s) | 551 |
| BmsI | GCATC | 2 cut(s) | 361, 394 |
| BpiI | GAAGAC | 1 cut(s) | 146 |
| BsaJI | CCNNGG | 1 cut(s) | 379 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 566 |
| BseDI | CCNNGG | 1 cut(s) | 379 |
| BseGI | GGATG | 3 cut(s) | 205, 409, 424 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 566 |
| BseXI | GCAGC | 3 cut(s) | 253, 324, 327 |
| BseYI | CCCAGC | 1 cut(s) | 220 |
| BsgI | GTGCAG | 1 cut(s) | 252 |
| BshNI | GGYRCC | 1 cut(s) | 549 |
| BslFI | GGGAC | 2 cut(s) | 203, 340 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 566 |
| BsmFI | GGGAC | 2 cut(s) | 203, 340 |
| BsmI | GAATGC | 2 cut(s) | 374, 541 |
| Bsp143I | GATC | 1 cut(s) | 577 |
| Bsp19I | CCATGG | 1 cut(s) | 379 |
| BspACI | CCGC | 3 cut(s) | 17, 532, 539 |
| BspLI | GGNNCC | 1 cut(s) | 551 |
| BspMAI | CTGCAG | 1 cut(s) | 345 |
| BspPI | GGATC | 1 cut(s) | 585 |
| BspT107I | GGYRCC | 1 cut(s) | 549 |
| BssECI | CCNNGG | 1 cut(s) | 379 |
| BssMI | GATC | 1 cut(s) | 577 |
| BssT1I | CCWWGG | 1 cut(s) | 379 |
| Bst6I | CTCTTC | 2 cut(s) | 201, 221 |
| BstC8I | GCNNGC | 1 cut(s) | 278 |
| BstDEI | CTNAG | 1 cut(s) | 111 |
| BstDSI | CCRYGG | 1 cut(s) | 379 |
| BstF5I | GGATG | 3 cut(s) | 205, 409, 424 |
| BstKTI | GATC | 1 cut(s) | 580 |
| BstMBI | GATC | 1 cut(s) | 577 |
| BstSFI | CTRYAG | 1 cut(s) | 341 |
| BstV1I | GCAGC | 3 cut(s) | 253, 324, 327 |
| BstV2I | GAAGAC | 1 cut(s) | 146 |
| BtgI | CCRYGG | 1 cut(s) | 379 |
| BtsCI | GGATG | 3 cut(s) | 205, 409, 424 |
| Cac8I | GCNNGC | 1 cut(s) | 278 |
| Csp6I | GTAC | 2 cut(s) | 156, 550 |
| CviAII | CATG | 2 cut(s) | 316, 380 |
| CviJI | RGCY | 3 cut(s) | 86, 337, 446 |
| CviKI_1 | RGCY | 3 cut(s) | 86, 337, 446 |
| CviQI | GTAC | 2 cut(s) | 156, 550 |
| DdeI | CTNAG | 1 cut(s) | 111 |
| DpnI | GATC | 1 cut(s) | 579 |
| DpnII | GATC | 1 cut(s) | 577 |
| Eam1104I | CTCTTC | 2 cut(s) | 201, 221 |
| EarI | CTCTTC | 2 cut(s) | 201, 221 |
| Eco130I | CCWWGG | 1 cut(s) | 379 |
| Eco57I | CTGAAG | 1 cut(s) | 108 |
| EcoT14I | CCWWGG | 1 cut(s) | 379 |
| EcoT22I | ATGCAT | 1 cut(s) | 376 |
| ErhI | CCWWGG | 1 cut(s) | 379 |
| FaeI | CATG | 2 cut(s) | 319, 383 |
| FaiI | YATR | 8 cut(s) | 240, 255, 317, 381, 463, 512, 518, 573 |
| FaqI | GGGAC | 2 cut(s) | 203, 340 |
| FatI | CATG | 2 cut(s) | 315, 379 |
| FauI | CCCGC | 1 cut(s) | 532 |
| Fnu4HI | GCNGC | 3 cut(s) | 267, 338, 341 |
| FokI | GGATG | 3 cut(s) | 212, 416, 431 |
| Fsp4HI | GCNGC | 3 cut(s) | 267, 338, 341 |
| GluI | GCNGC | 3 cut(s) | 267, 338, 341 |
| GsaI | CCCAGC | 1 cut(s) | 224 |
| Hin1II | CATG | 2 cut(s) | 319, 383 |
| HindIII | AAGCTT | 1 cut(s) | 444 |
| Hpy188I | TCNGA | 2 cut(s) | 418, 494 |
| Hpy188III | TCNNGA | 1 cut(s) | 587 |
| Hpy99I | CGWCG | 1 cut(s) | 182 |
| HpyAV | CCTTC | 3 cut(s) | 160, 317, 559 |
| HpyCH4V | TGCA | 5 cut(s) | 269, 343, 359, 374, 407 |
| HpyF3I | CTNAG | 1 cut(s) | 111 |
| Hsp92II | CATG | 2 cut(s) | 319, 383 |
| KpnI | GGTACC | 1 cut(s) | 553 |
| Kzo9I | GATC | 1 cut(s) | 577 |
| LpnPI | CCDG | 1 cut(s) | 234 |
| Lsp1109I | GCAGC | 3 cut(s) | 253, 324, 327 |
| LweI | GCATC | 2 cut(s) | 361, 394 |
| MaeIII | GTNAC | 1 cut(s) | 587 |
| MalI | GATC | 1 cut(s) | 579 |
| MboI | GATC | 1 cut(s) | 577 |
| MfeI | CAATTG | 1 cut(s) | 293 |
| MluCI | AATT | 3 cut(s) | 293, 390, 488 |
| MnlI | CCTC | 5 cut(s) | 69, 175, 202, 241, 377 |
| Mph1103I | ATGCAT | 1 cut(s) | 376 |
| MroXI | GAANNNNTTC | 1 cut(s) | 102 |
| MseI | TTAA | 2 cut(s) | 393, 425 |
| MspA1I | CMGCKG | 1 cut(s) | 17 |
| MunI | CAATTG | 1 cut(s) | 293 |
| Mva1269I | GAATGC | 2 cut(s) | 374, 541 |
| NcoI | CCATGG | 1 cut(s) | 379 |
| NdeII | GATC | 1 cut(s) | 577 |
| NlaIII | CATG | 2 cut(s) | 319, 383 |
| NlaIV | GGNNCC | 1 cut(s) | 551 |
| NmuCI | GTSAC | 1 cut(s) | 587 |
| NsiI | ATGCAT | 1 cut(s) | 376 |
| PctI | GAATGC | 2 cut(s) | 374, 541 |
| PdmI | GAANNNNTTC | 1 cut(s) | 102 |
| PkrI | GCNGC | 3 cut(s) | 268, 339, 342 |
| PspFI | CCCAGC | 1 cut(s) | 220 |
| PspN4I | GGNNCC | 1 cut(s) | 551 |
| PstI | CTGCAG | 1 cut(s) | 345 |
| RsaI | GTAC | 2 cut(s) | 157, 551 |
| RsaNI | GTAC | 2 cut(s) | 156, 550 |
| SaqAI | TTAA | 2 cut(s) | 393, 425 |
| SatI | GCNGC | 3 cut(s) | 267, 338, 341 |
| Sau3AI | GATC | 1 cut(s) | 577 |
| SetI | ASST | 5 cut(s) | 88, 339, 388, 448, 468 |
| SfaNI | GCATC | 2 cut(s) | 361, 394 |
| SfcI | CTRYAG | 1 cut(s) | 341 |
| Sse9I | AATT | 3 cut(s) | 293, 390, 488 |
| SsiI | CCGC | 3 cut(s) | 17, 532, 539 |
| StyI | CCWWGG | 1 cut(s) | 379 |
| TaqI | TCGA | 1 cut(s) | 580 |
| TasI | AATT | 3 cut(s) | 293, 390, 488 |
| TatI | WGTACW | 1 cut(s) | 155 |
| Tru1I | TTAA | 2 cut(s) | 393, 425 |
| Tru9I | TTAA | 2 cut(s) | 393, 425 |
| TseFI | GTSAC | 1 cut(s) | 587 |
| TseI | GCWGC | 3 cut(s) | 266, 337, 340 |
| Tsp45I | GTSAC | 1 cut(s) | 587 |
| TspDTI | ATGAA | 3 cut(s) | 17, 108, 219 |
| TspGWI | ACGGA | 1 cut(s) | 590 |
| XapI | RAATTY | 1 cut(s) | 488 |
| XmnI | GAANNNNTTC | 1 cut(s) | 102 |
| Zsp2I | ATGCAT | 1 cut(s) | 376 |