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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 141 |
| AfaI | GTAC | 2 cut(s) | 31, 331 |
| AgsI | TTSAA | 1 cut(s) | 264 |
| AluBI | AGCT | 2 cut(s) | 211, 458 |
| AluI | AGCT | 2 cut(s) | 211, 458 |
| ApeKI | GCWGC | 2 cut(s) | 211, 455 |
| AspLEI | GCGC | 1 cut(s) | 455 |
| AsuHPI | GGTGA | 2 cut(s) | 358, 387 |
| BbsI | GAAGAC | 1 cut(s) | 20 |
| BbvI | GCAGC | 1 cut(s) | 198 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 270, 304 |
| BfaI | CTAG | 1 cut(s) | 339 |
| BisI | GCNGC | 3 cut(s) | 141, 212, 456 |
| BlpI | GCTNAGC | 1 cut(s) | 449 |
| BlsI | GCNGC | 3 cut(s) | 142, 213, 457 |
| BmsI | GCATC | 2 cut(s) | 235, 268 |
| BpiI | GAAGAC | 1 cut(s) | 20 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 449 |
| BsaJI | CCNNGG | 1 cut(s) | 253 |
| BseDI | CCNNGG | 1 cut(s) | 253 |
| BseGI | GGATG | 3 cut(s) | 79, 283, 298 |
| BseXI | GCAGC | 1 cut(s) | 198 |
| BseYI | CCCAGC | 1 cut(s) | 94 |
| BslFI | GGGAC | 2 cut(s) | 77, 214 |
| BsmFI | GGGAC | 2 cut(s) | 77, 214 |
| BsmI | GAATGC | 1 cut(s) | 248 |
| Bsp143I | GATC | 1 cut(s) | 341 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 449 |
| Bsp19I | CCATGG | 1 cut(s) | 253 |
| BspACI | CCGC | 1 cut(s) | 141 |
| BssECI | CCNNGG | 1 cut(s) | 253 |
| BssMI | GATC | 1 cut(s) | 341 |
| BssT1I | CCWWGG | 1 cut(s) | 253 |
| Bst4CI | ACNGT | 3 cut(s) | 370, 382, 399 |
| Bst6I | CTCTTC | 2 cut(s) | 75, 95 |
| BstC8I | GCNNGC | 1 cut(s) | 152 |
| BstDEI | CTNAG | 1 cut(s) | 449 |
| BstDSI | CCRYGG | 1 cut(s) | 253 |
| BstF5I | GGATG | 3 cut(s) | 79, 283, 298 |
| BstHHI | GCGC | 1 cut(s) | 455 |
| BstKTI | GATC | 1 cut(s) | 344 |
| BstMBI | GATC | 1 cut(s) | 341 |
| BstV1I | GCAGC | 1 cut(s) | 198 |
| BstV2I | GAAGAC | 1 cut(s) | 20 |
| BtgI | CCRYGG | 1 cut(s) | 253 |
| BtsCI | GGATG | 3 cut(s) | 79, 283, 298 |
| Cac8I | GCNNGC | 1 cut(s) | 152 |
| CfoI | GCGC | 1 cut(s) | 455 |
| Csp6I | GTAC | 2 cut(s) | 30, 330 |
| CviAII | CATG | 3 cut(s) | 190, 254, 350 |
| CviJI | RGCY | 3 cut(s) | 211, 320, 458 |
| CviKI_1 | RGCY | 3 cut(s) | 211, 320, 458 |
| CviQI | GTAC | 2 cut(s) | 30, 330 |
| DdeI | CTNAG | 1 cut(s) | 449 |
| DpnI | GATC | 1 cut(s) | 343 |
| DpnII | GATC | 1 cut(s) | 341 |
| Eam1104I | CTCTTC | 2 cut(s) | 75, 95 |
| EarI | CTCTTC | 2 cut(s) | 75, 95 |
| Eco130I | CCWWGG | 1 cut(s) | 253 |
| EcoT14I | CCWWGG | 1 cut(s) | 253 |
| EcoT22I | ATGCAT | 1 cut(s) | 250 |
| ErhI | CCWWGG | 1 cut(s) | 253 |
| FaeI | CATG | 3 cut(s) | 193, 257, 353 |
| FaqI | GGGAC | 2 cut(s) | 77, 214 |
| FatI | CATG | 3 cut(s) | 189, 253, 349 |
| FauNDI | CATATG | 1 cut(s) | 414 |
| Fnu4HI | GCNGC | 3 cut(s) | 141, 212, 456 |
| FokI | GGATG | 3 cut(s) | 86, 290, 305 |
| Fsp4HI | GCNGC | 3 cut(s) | 141, 212, 456 |
| FspBI | CTAG | 1 cut(s) | 339 |
| GlaI | GCGC | 1 cut(s) | 454 |
| GluI | GCNGC | 3 cut(s) | 141, 212, 456 |
| GsaI | CCCAGC | 1 cut(s) | 98 |
| HhaI | GCGC | 1 cut(s) | 455 |
| Hin1II | CATG | 3 cut(s) | 193, 257, 353 |
| Hin6I | GCGC | 1 cut(s) | 453 |
| HinP1I | GCGC | 1 cut(s) | 453 |
| HphI | GGTGA | 2 cut(s) | 358, 387 |
| Hpy188I | TCNGA | 2 cut(s) | 161, 292 |
| Hpy99I | CGWCG | 1 cut(s) | 56 |
| HpyAV | CCTTC | 2 cut(s) | 34, 191 |
| HpyCH4III | ACNGT | 3 cut(s) | 370, 382, 399 |
| HpyCH4V | TGCA | 4 cut(s) | 233, 248, 281, 412 |
| HpyF3I | CTNAG | 1 cut(s) | 449 |
| Hsp92II | CATG | 3 cut(s) | 193, 257, 353 |
| HspAI | GCGC | 1 cut(s) | 453 |
| Kzo9I | GATC | 1 cut(s) | 341 |
| LpnPI | CCDG | 1 cut(s) | 108 |
| Lsp1109I | GCAGC | 1 cut(s) | 198 |
| LweI | GCATC | 2 cut(s) | 235, 268 |
| MaeI | CTAG | 1 cut(s) | 339 |
| MaeIII | GTNAC | 3 cut(s) | 364, 376, 393 |
| MalI | GATC | 1 cut(s) | 343 |
| MboI | GATC | 1 cut(s) | 341 |
| MboII | GAAGA | 7 cut(s) | 25, 49, 56, 71, 74, 92, 112 |
| MfeI | CAATTG | 1 cut(s) | 167 |
| MluCI | AATT | 2 cut(s) | 167, 264 |
| MnlI | CCTC | 4 cut(s) | 49, 76, 115, 251 |
| Mph1103I | ATGCAT | 1 cut(s) | 250 |
| MseI | TTAA | 3 cut(s) | 267, 299, 372 |
| MspA1I | CMGCKG | 1 cut(s) | 458 |
| MunI | CAATTG | 1 cut(s) | 167 |
| Mva1269I | GAATGC | 1 cut(s) | 248 |
| NcoI | CCATGG | 1 cut(s) | 253 |
| NdeI | CATATG | 1 cut(s) | 414 |
| NdeII | GATC | 1 cut(s) | 341 |
| NlaIII | CATG | 3 cut(s) | 193, 257, 353 |
| NmuCI | GTSAC | 2 cut(s) | 364, 393 |
| NsiI | ATGCAT | 1 cut(s) | 250 |
| PctI | GAATGC | 1 cut(s) | 248 |
| PkrI | GCNGC | 3 cut(s) | 142, 213, 457 |
| PspFI | CCCAGC | 1 cut(s) | 94 |
| PvuII | CAGCTG | 1 cut(s) | 458 |
| RsaI | GTAC | 2 cut(s) | 31, 331 |
| RsaNI | GTAC | 2 cut(s) | 30, 330 |
| SaqAI | TTAA | 3 cut(s) | 267, 299, 372 |
| SatI | GCNGC | 3 cut(s) | 141, 212, 456 |
| Sau3AI | GATC | 1 cut(s) | 341 |
| SetI | ASST | 3 cut(s) | 213, 262, 460 |
| SfaNI | GCATC | 2 cut(s) | 235, 268 |
| Sse9I | AATT | 2 cut(s) | 167, 264 |
| SsiI | CCGC | 1 cut(s) | 141 |
| SspMI | CTAG | 1 cut(s) | 339 |
| StyI | CCWWGG | 1 cut(s) | 253 |
| TaaI | ACNGT | 3 cut(s) | 370, 382, 399 |
| TaqII | GACCGA | 1 cut(s) | 209 |
| TasI | AATT | 2 cut(s) | 167, 264 |
| TatI | WGTACW | 2 cut(s) | 29, 329 |
| TauI | GCSGC | 1 cut(s) | 143 |
| Tru1I | TTAA | 3 cut(s) | 267, 299, 372 |
| Tru9I | TTAA | 3 cut(s) | 267, 299, 372 |
| TseFI | GTSAC | 2 cut(s) | 364, 393 |
| TseI | GCWGC | 2 cut(s) | 211, 455 |
| Tsp45I | GTSAC | 2 cut(s) | 364, 393 |
| TspDTI | ATGAA | 2 cut(s) | 93, 430 |
| XspI | CTAG | 1 cut(s) | 339 |
| Zsp2I | ATGCAT | 1 cut(s) | 250 |