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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 285 |
| AclWI | GGATC | 1 cut(s) | 295 |
| AcsI | RAATTY | 1 cut(s) | 243 |
| AgsI | TTSAA | 3 cut(s) | 67, 335, 575 |
| AjiI | CACGTC | 1 cut(s) | 116 |
| AjnI | CCWGG | 2 cut(s) | 118, 737 |
| AluBI | AGCT | 2 cut(s) | 353, 617 |
| AluI | AGCT | 2 cut(s) | 353, 617 |
| Alw26I | GTCTC | 1 cut(s) | 704 |
| AlwI | GGATC | 1 cut(s) | 295 |
| AoxI | GGCC | 2 cut(s) | 183, 673 |
| ApeKI | GCWGC | 1 cut(s) | 353 |
| ApoI | RAATTY | 1 cut(s) | 243 |
| AspS9I | GGNCC | 1 cut(s) | 184 |
| AsuHPI | GGTGA | 5 cut(s) | 134, 318, 360, 537, 616 |
| AxyI | CCTNAGG | 1 cut(s) | 189 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 512, 545 |
| BbvI | GCAGC | 1 cut(s) | 340 |
| BceAI | ACGGC | 1 cut(s) | 294 |
| BciT130I | CCWGG | 2 cut(s) | 120, 739 |
| BcoDI | GTCTC | 1 cut(s) | 704 |
| BfaI | CTAG | 2 cut(s) | 159, 267 |
| BfmI | CTRYAG | 1 cut(s) | 291 |
| BisI | GCNGC | 1 cut(s) | 354 |
| BlsI | GCNGC | 1 cut(s) | 355 |
| Bme1390I | CCNGG | 2 cut(s) | 120, 739 |
| BmgBI | CACGTC | 1 cut(s) | 116 |
| BmgT120I | GGNCC | 1 cut(s) | 184 |
| BmiI | GGNNCC | 1 cut(s) | 186 |
| BmrFI | CCNGG | 2 cut(s) | 120, 739 |
| BmsI | GCATC | 1 cut(s) | 397 |
| BoxI | GACNNNNGTC | 1 cut(s) | 322 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 23, 55 |
| BpmI | CTGGAG | 1 cut(s) | 444 |
| BsaJI | CCNNGG | 1 cut(s) | 276 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 130, 160, 466, 496 |
| Bse1I | ACTGG | 2 cut(s) | 12, 496 |
| Bse21I | CCTNAGG | 1 cut(s) | 189 |
| BseBI | CCWGG | 2 cut(s) | 120, 739 |
| BseDI | CCNNGG | 1 cut(s) | 276 |
| BseMII | CTCAG | 4 cut(s) | 81, 203, 609, 675 |
| BseNI | ACTGG | 2 cut(s) | 12, 496 |
| BseXI | GCAGC | 1 cut(s) | 340 |
| BsgI | GTGCAG | 1 cut(s) | 339 |
| BshFI | GGCC | 2 cut(s) | 185, 675 |
| BsmAI | GTCTC | 1 cut(s) | 704 |
| BsmI | GAATGC | 1 cut(s) | 341 |
| BsnI | GGCC | 2 cut(s) | 185, 675 |
| Bsp143I | GATC | 2 cut(s) | 287, 534 |
| BspACI | CCGC | 1 cut(s) | 285 |
| BspANI | GGCC | 2 cut(s) | 185, 675 |
| BspCNI | CTCAG | 4 cut(s) | 82, 202, 610, 674 |
| BspLI | GGNNCC | 1 cut(s) | 186 |
| BspMAI | CTGCAG | 1 cut(s) | 295 |
| BspPI | GGATC | 1 cut(s) | 295 |
| BsrI | ACTGG | 2 cut(s) | 12, 496 |
| BssECI | CCNNGG | 1 cut(s) | 276 |
| BssMI | GATC | 2 cut(s) | 287, 534 |
| Bst2UI | CCWGG | 2 cut(s) | 120, 739 |
| Bst4CI | ACNGT | 1 cut(s) | 715 |
| BstC8I | GCNNGC | 3 cut(s) | 212, 283, 412 |
| BstDEI | CTNAG | 4 cut(s) | 90, 189, 618, 661 |
| BstDSI | CCRYGG | 1 cut(s) | 276 |
| BstKTI | GATC | 2 cut(s) | 290, 537 |
| BstMAI | GTCTC | 1 cut(s) | 704 |
| BstMBI | GATC | 2 cut(s) | 287, 534 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 350 |
| BstNI | CCWGG | 2 cut(s) | 120, 739 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 322 |
| BstSCI | CCNGG | 2 cut(s) | 118, 737 |
| BstSFI | CTRYAG | 1 cut(s) | 291 |
| BstV1I | GCAGC | 1 cut(s) | 340 |
| BstX2I | RGATCY | 1 cut(s) | 287 |
| BstYI | RGATCY | 1 cut(s) | 287 |
| Bsu36I | CCTNAGG | 1 cut(s) | 189 |
| BsuRI | GGCC | 2 cut(s) | 185, 675 |
| BtgI | CCRYGG | 1 cut(s) | 276 |
| BtrI | CACGTC | 1 cut(s) | 116 |
| BtsIMutI | CAGTG | 1 cut(s) | 41 |
| Cac8I | GCNNGC | 3 cut(s) | 212, 283, 412 |
| Cfr13I | GGNCC | 1 cut(s) | 184 |
| CseI | GACGC | 1 cut(s) | 473 |
| CviJI | RGCY | 9 cut(s) | 38, 185, 199, 353, 520, 559, 617, 631, 675 |
| CviKI_1 | RGCY | 9 cut(s) | 38, 185, 199, 353, 520, 559, 617, 631, 675 |
| DdeI | CTNAG | 4 cut(s) | 90, 189, 618, 661 |
| DpnI | GATC | 2 cut(s) | 289, 536 |
| DpnII | GATC | 2 cut(s) | 287, 534 |
| Eco81I | CCTNAGG | 1 cut(s) | 189 |
| EcoO109I | RGGNCCY | 1 cut(s) | 184 |
| EcoRI | GAATTC | 1 cut(s) | 243 |
| EcoRII | CCWGG | 2 cut(s) | 118, 737 |
| EcoT22I | ATGCAT | 1 cut(s) | 442 |
| FaiI | YATR | 7 cut(s) | 74, 99, 509, 597, 647, 687, 732 |
| Fnu4HI | GCNGC | 1 cut(s) | 354 |
| Fsp4HI | GCNGC | 1 cut(s) | 354 |
| FspBI | CTAG | 2 cut(s) | 159, 267 |
| GluI | GCNGC | 1 cut(s) | 354 |
| GsuI | CTGGAG | 1 cut(s) | 444 |
| HaeIII | GGCC | 2 cut(s) | 185, 675 |
| HgaI | GACGC | 1 cut(s) | 473 |
| HinfI | GANTC | 6 cut(s) | 101, 134, 272, 365, 659, 665 |
| HphI | GGTGA | 5 cut(s) | 134, 318, 360, 537, 616 |
| Hpy166II | GTNNAC | 1 cut(s) | 624 |
| Hpy188I | TCNGA | 3 cut(s) | 235, 534, 664 |
| Hpy188III | TCNNGA | 2 cut(s) | 191, 461 |
| Hpy8I | GTNNAC | 1 cut(s) | 624 |
| HpyAV | CCTTC | 2 cut(s) | 61, 497 |
| HpyCH4III | ACNGT | 1 cut(s) | 715 |
| HpyCH4IV | ACGT | 2 cut(s) | 115, 570 |
| HpyCH4V | TGCA | 5 cut(s) | 25, 293, 356, 414, 440 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 350 |
| HpyF3I | CTNAG | 4 cut(s) | 90, 189, 618, 661 |
| HpySE526I | ACGT | 2 cut(s) | 115, 570 |
| Kzo9I | GATC | 2 cut(s) | 287, 534 |
| Lsp1109I | GCAGC | 1 cut(s) | 340 |
| LweI | GCATC | 1 cut(s) | 397 |
| MaeI | CTAG | 2 cut(s) | 159, 267 |
| MaeII | ACGT | 2 cut(s) | 115, 570 |
| MaeIII | GTNAC | 2 cut(s) | 3, 324 |
| MalI | GATC | 2 cut(s) | 289, 536 |
| MboI | GATC | 2 cut(s) | 287, 534 |
| MfeI | CAATTG | 2 cut(s) | 62, 583 |
| MflI | RGATCY | 1 cut(s) | 287 |
| MluCI | AATT | 6 cut(s) | 27, 62, 94, 243, 389, 583 |
| MlyI | GAGTC | 4 cut(s) | 110, 128, 266, 653 |
| MmeI | TCCRAC | 1 cut(s) | 480 |
| Mph1103I | ATGCAT | 1 cut(s) | 442 |
| MseI | TTAA | 3 cut(s) | 48, 387, 435 |
| MslI | CAYNNNNRTG | 2 cut(s) | 77, 334 |
| MspR9I | CCNGG | 2 cut(s) | 120, 739 |
| MunI | CAATTG | 2 cut(s) | 62, 583 |
| Mva1269I | GAATGC | 1 cut(s) | 341 |
| MvaI | CCWGG | 2 cut(s) | 120, 739 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 350 |
| NdeII | GATC | 2 cut(s) | 287, 534 |
| NlaIV | GGNNCC | 1 cut(s) | 186 |
| NmuCI | GTSAC | 1 cut(s) | 324 |
| NsiI | ATGCAT | 1 cut(s) | 442 |
| PctI | GAATGC | 1 cut(s) | 341 |
| PfeI | GAWTC | 2 cut(s) | 365, 665 |
| PkrI | GCNGC | 1 cut(s) | 355 |
| PleI | GAGTC | 4 cut(s) | 109, 128, 266, 653 |
| PpsI | GAGTC | 4 cut(s) | 109, 128, 266, 653 |
| PshAI | GACNNNNGTC | 1 cut(s) | 322 |
| Psp6I | CCWGG | 2 cut(s) | 118, 737 |
| PspGI | CCWGG | 2 cut(s) | 118, 737 |
| PspN4I | GGNNCC | 1 cut(s) | 186 |
| PspPI | GGNCC | 1 cut(s) | 184 |
| PstI | CTGCAG | 1 cut(s) | 295 |
| PsuI | RGATCY | 1 cut(s) | 287 |
| RseI | CAYNNNNRTG | 2 cut(s) | 77, 334 |
| SaqAI | TTAA | 3 cut(s) | 48, 387, 435 |
| SatI | GCNGC | 1 cut(s) | 354 |
| Sau3AI | GATC | 2 cut(s) | 287, 534 |
| Sau96I | GGNCC | 1 cut(s) | 184 |
| SchI | GAGTC | 4 cut(s) | 110, 128, 266, 653 |
| ScrFI | CCNGG | 2 cut(s) | 120, 739 |
| SfaNI | GCATC | 1 cut(s) | 397 |
| SfcI | CTRYAG | 1 cut(s) | 291 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 77, 334 |
| Sse9I | AATT | 6 cut(s) | 27, 62, 94, 243, 389, 583 |
| SsiI | CCGC | 1 cut(s) | 285 |
| SspI | AATATT | 1 cut(s) | 636 |
| SspMI | CTAG | 2 cut(s) | 159, 267 |
| StyD4I | CCNGG | 2 cut(s) | 118, 737 |
| TaaI | ACNGT | 1 cut(s) | 715 |
| TaiI | ACGT | 2 cut(s) | 118, 573 |
| TasI | AATT | 6 cut(s) | 27, 62, 94, 243, 389, 583 |
| TfiI | GAWTC | 2 cut(s) | 365, 665 |
| Tru1I | TTAA | 3 cut(s) | 48, 387, 435 |
| Tru9I | TTAA | 3 cut(s) | 48, 387, 435 |
| TscAI | CASTG | 1 cut(s) | 48 |
| TseFI | GTSAC | 1 cut(s) | 324 |
| TseI | GCWGC | 1 cut(s) | 353 |
| Tsp45I | GTSAC | 1 cut(s) | 324 |
| TspRI | CASTG | 1 cut(s) | 48 |
| XapI | RAATTY | 1 cut(s) | 243 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 426 |
| XspI | CTAG | 2 cut(s) | 159, 267 |
| Zsp2I | ATGCAT | 1 cut(s) | 442 |