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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 152 |
| AccIII | TCCGGA | 1 cut(s) | 541 |
| AclWI | GGATC | 4 cut(s) | 251, 262, 321, 561 |
| AcsI | RAATTY | 1 cut(s) | 236 |
| AfaI | GTAC | 1 cut(s) | 11 |
| AgsI | TTSAA | 5 cut(s) | 37, 264, 353, 467, 643 |
| AluBI | AGCT | 1 cut(s) | 639 |
| AluI | AGCT | 1 cut(s) | 639 |
| Alw21I | GWGCWC | 1 cut(s) | 20 |
| Alw44I | GTGCAC | 1 cut(s) | 16 |
| AlwI | GGATC | 4 cut(s) | 251, 262, 321, 561 |
| Ama87I | CYCGRG | 2 cut(s) | 89, 187 |
| Aor13HI | TCCGGA | 1 cut(s) | 541 |
| ApaLI | GTGCAC | 1 cut(s) | 16 |
| ApoI | RAATTY | 1 cut(s) | 236 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 236 |
| AspA2I | CCTAGG | 1 cut(s) | 271 |
| AspS9I | GGNCC | 1 cut(s) | 298 |
| AsuHPI | GGTGA | 2 cut(s) | 90, 302 |
| AsuII | TTCGAA | 1 cut(s) | 614 |
| AvaI | CYCGRG | 2 cut(s) | 89, 187 |
| AvaII | GGWCC | 1 cut(s) | 298 |
| AvrII | CCTAGG | 1 cut(s) | 271 |
| BaeGI | GKGCMC | 1 cut(s) | 20 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 566, 598 |
| Bbv12I | GWGCWC | 1 cut(s) | 20 |
| BceAI | ACGGC | 1 cut(s) | 364 |
| BfaI | CTAG | 1 cut(s) | 272 |
| BlnI | CCTAGG | 1 cut(s) | 271 |
| Bme18I | GGWCC | 1 cut(s) | 298 |
| BmeT110I | CYCGRG | 2 cut(s) | 89, 187 |
| BmgT120I | GGNCC | 1 cut(s) | 298 |
| BmiI | GGNNCC | 1 cut(s) | 300 |
| BmsI | GCATC | 2 cut(s) | 109, 390 |
| Bpu14I | TTCGAA | 1 cut(s) | 614 |
| Bsa29I | ATCGAT | 2 cut(s) | 164, 325 |
| BsaJI | CCNNGG | 4 cut(s) | 127, 271, 312, 380 |
| BsaWI | WCCGGW | 1 cut(s) | 541 |
| BseAI | TCCGGA | 1 cut(s) | 541 |
| BseCI | ATCGAT | 2 cut(s) | 164, 325 |
| BseDI | CCNNGG | 4 cut(s) | 127, 271, 312, 380 |
| BseGI | GGATG | 1 cut(s) | 308 |
| BseRI | GAGGAG | 4 cut(s) | 158, 491, 666, 669 |
| BseSI | GKGCMC | 1 cut(s) | 20 |
| BsgI | GTGCAG | 1 cut(s) | 25 |
| BshVI | ATCGAT | 2 cut(s) | 164, 325 |
| BsiHKAI | GWGCWC | 1 cut(s) | 20 |
| BsiHKCI | CYCGRG | 2 cut(s) | 89, 187 |
| BsiSI | CCGG | 1 cut(s) | 542 |
| BslFI | GGGAC | 1 cut(s) | 284 |
| BsmFI | GGGAC | 1 cut(s) | 284 |
| BsmI | GAATGC | 3 cut(s) | 122, 282, 393 |
| BsoBI | CYCGRG | 2 cut(s) | 89, 187 |
| Bsp119I | TTCGAA | 1 cut(s) | 614 |
| Bsp1286I | GDGCHC | 1 cut(s) | 20 |
| Bsp13I | TCCGGA | 1 cut(s) | 541 |
| Bsp143I | GATC | 6 cut(s) | 243, 267, 326, 404, 553, 670 |
| Bsp19I | CCATGG | 1 cut(s) | 127 |
| BspDI | ATCGAT | 2 cut(s) | 164, 325 |
| BspEI | TCCGGA | 1 cut(s) | 541 |
| BspLI | GGNNCC | 1 cut(s) | 300 |
| BspPI | GGATC | 4 cut(s) | 251, 262, 321, 561 |
| BspT104I | TTCGAA | 1 cut(s) | 614 |
| BssECI | CCNNGG | 4 cut(s) | 127, 271, 312, 380 |
| BssMI | GATC | 6 cut(s) | 243, 267, 326, 404, 553, 670 |
| BssT1I | CCWWGG | 2 cut(s) | 127, 271 |
| Bst4CI | ACNGT | 1 cut(s) | 538 |
| Bst6I | CTCTTC | 3 cut(s) | 221, 501, 543 |
| BstBI | TTCGAA | 1 cut(s) | 614 |
| BstDSI | CCRYGG | 2 cut(s) | 127, 380 |
| BstF5I | GGATG | 1 cut(s) | 308 |
| BstKTI | GATC | 6 cut(s) | 246, 270, 329, 407, 556, 673 |
| BstMBI | GATC | 6 cut(s) | 243, 267, 326, 404, 553, 670 |
| BstSLI | GKGCMC | 1 cut(s) | 20 |
| BstX2I | RGATCY | 2 cut(s) | 243, 267 |
| BstYI | RGATCY | 2 cut(s) | 243, 267 |
| Bsu15I | ATCGAT | 2 cut(s) | 164, 325 |
| BsuTUI | ATCGAT | 2 cut(s) | 164, 325 |
| BtgI | CCRYGG | 2 cut(s) | 127, 380 |
| BtsCI | GGATG | 1 cut(s) | 308 |
| Cfr13I | GGNCC | 1 cut(s) | 298 |
| ClaI | ATCGAT | 2 cut(s) | 164, 325 |
| Csp6I | GTAC | 1 cut(s) | 10 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 574, 609 |
| CviAII | CATG | 3 cut(s) | 128, 203, 472 |
| CviJI | RGCY | 3 cut(s) | 135, 379, 639 |
| CviKI_1 | RGCY | 3 cut(s) | 135, 379, 639 |
| CviQI | GTAC | 1 cut(s) | 10 |
| DpnI | GATC | 6 cut(s) | 245, 269, 328, 406, 555, 672 |
| DpnII | GATC | 6 cut(s) | 243, 267, 326, 404, 553, 670 |
| Eam1104I | CTCTTC | 3 cut(s) | 221, 501, 543 |
| EarI | CTCTTC | 3 cut(s) | 221, 501, 543 |
| Eco130I | CCWWGG | 2 cut(s) | 127, 271 |
| Eco47I | GGWCC | 1 cut(s) | 298 |
| Eco88I | CYCGRG | 2 cut(s) | 89, 187 |
| EcoT14I | CCWWGG | 2 cut(s) | 127, 271 |
| EcoT22I | ATGCAT | 1 cut(s) | 124 |
| ErhI | CCWWGG | 2 cut(s) | 127, 271 |
| FaeI | CATG | 3 cut(s) | 131, 206, 475 |
| FaiI | YATR | 7 cut(s) | 129, 140, 204, 322, 473, 667, 669 |
| FaqI | GGGAC | 1 cut(s) | 284 |
| FatI | CATG | 3 cut(s) | 127, 202, 471 |
| FblI | GTMKAC | 1 cut(s) | 152 |
| FokI | GGATG | 1 cut(s) | 295 |
| FspBI | CTAG | 1 cut(s) | 272 |
| HapII | CCGG | 1 cut(s) | 542 |
| Hin1II | CATG | 3 cut(s) | 131, 206, 475 |
| HincII | GTYRAC | 1 cut(s) | 153 |
| HindII | GTYRAC | 1 cut(s) | 153 |
| HinfI | GANTC | 3 cut(s) | 61, 491, 497 |
| HpaII | CCGG | 1 cut(s) | 542 |
| HphI | GGTGA | 2 cut(s) | 90, 302 |
| Hpy166II | GTNNAC | 2 cut(s) | 18, 153 |
| Hpy188I | TCNGA | 4 cut(s) | 313, 331, 496, 502 |
| Hpy188III | TCNNGA | 4 cut(s) | 34, 241, 247, 542 |
| Hpy8I | GTNNAC | 2 cut(s) | 18, 153 |
| HpyAV | CCTTC | 2 cut(s) | 425, 439 |
| HpyCH4III | ACNGT | 1 cut(s) | 538 |
| HpyCH4V | TGCA | 6 cut(s) | 6, 18, 71, 122, 280, 396 |
| Hsp92II | CATG | 3 cut(s) | 131, 206, 475 |
| Kpn2I | TCCGGA | 1 cut(s) | 541 |
| Kzo9I | GATC | 6 cut(s) | 243, 267, 326, 404, 553, 670 |
| LmnI | GCTCC | 1 cut(s) | 317 |
| LpnPI | CCDG | 2 cut(s) | 226, 555 |
| LweI | GCATC | 2 cut(s) | 109, 390 |
| MaeI | CTAG | 1 cut(s) | 272 |
| MaeIII | GTNAC | 1 cut(s) | 96 |
| MalI | GATC | 6 cut(s) | 245, 269, 328, 406, 555, 672 |
| MboI | GATC | 6 cut(s) | 243, 267, 326, 404, 553, 670 |
| MboII | GAAGA | 9 cut(s) | 208, 211, 244, 454, 518, 557, 560, 583, 586 |
| MflI | RGATCY | 2 cut(s) | 243, 267 |
| MhlI | GDGCHC | 1 cut(s) | 20 |
| MluCI | AATT | 3 cut(s) | 236, 365, 558 |
| MmeI | TCCRAC | 2 cut(s) | 354, 442 |
| Mph1103I | ATGCAT | 1 cut(s) | 124 |
| MroI | TCCGGA | 1 cut(s) | 541 |
| MroXI | GAANNNNTTC | 1 cut(s) | 236 |
| MseI | TTAA | 3 cut(s) | 23, 45, 368 |
| MslI | CAYNNNNRTG | 1 cut(s) | 207 |
| MspI | CCGG | 1 cut(s) | 542 |
| Mva1269I | GAATGC | 3 cut(s) | 122, 282, 393 |
| NcoI | CCATGG | 1 cut(s) | 127 |
| NdeII | GATC | 6 cut(s) | 243, 267, 326, 404, 553, 670 |
| NlaIII | CATG | 3 cut(s) | 131, 206, 475 |
| NlaIV | GGNNCC | 1 cut(s) | 300 |
| NmuCI | GTSAC | 1 cut(s) | 96 |
| NsiI | ATGCAT | 1 cut(s) | 124 |
| NspV | TTCGAA | 1 cut(s) | 614 |
| PaeR7I | CTCGAG | 1 cut(s) | 89 |
| PctI | GAATGC | 3 cut(s) | 122, 282, 393 |
| PdmI | GAANNNNTTC | 1 cut(s) | 236 |
| PfeI | GAWTC | 3 cut(s) | 61, 491, 497 |
| PspN4I | GGNNCC | 1 cut(s) | 300 |
| PspPI | GGNCC | 1 cut(s) | 298 |
| PspXI | VCTCGAGB | 1 cut(s) | 89 |
| PsuI | RGATCY | 2 cut(s) | 243, 267 |
| RsaI | GTAC | 1 cut(s) | 11 |
| RsaNI | GTAC | 1 cut(s) | 10 |
| RseI | CAYNNNNRTG | 1 cut(s) | 207 |
| SalI | GTCGAC | 1 cut(s) | 151 |
| SaqAI | TTAA | 3 cut(s) | 23, 45, 368 |
| Sau3AI | GATC | 6 cut(s) | 243, 267, 326, 404, 553, 670 |
| Sau96I | GGNCC | 1 cut(s) | 298 |
| SduI | GDGCHC | 1 cut(s) | 20 |
| SetI | ASST | 5 cut(s) | 11, 17, 216, 292, 641 |
| SfaNI | GCATC | 2 cut(s) | 109, 390 |
| Sfr274I | CTCGAG | 1 cut(s) | 89 |
| SfuI | TTCGAA | 1 cut(s) | 614 |
| SinI | GGWCC | 1 cut(s) | 298 |
| SlaI | CTCGAG | 1 cut(s) | 89 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 207 |
| SmlI | CTYRAG | 1 cut(s) | 89 |
| SmoI | CTYRAG | 1 cut(s) | 89 |
| Sse9I | AATT | 3 cut(s) | 236, 365, 558 |
| SspI | AATATT | 1 cut(s) | 112 |
| SspMI | CTAG | 1 cut(s) | 272 |
| StyI | CCWWGG | 2 cut(s) | 127, 271 |
| TaaI | ACNGT | 1 cut(s) | 538 |
| TasI | AATT | 3 cut(s) | 236, 365, 558 |
| TfiI | GAWTC | 3 cut(s) | 61, 491, 497 |
| Tru1I | TTAA | 3 cut(s) | 23, 45, 368 |
| Tru9I | TTAA | 3 cut(s) | 23, 45, 368 |
| TseFI | GTSAC | 1 cut(s) | 96 |
| Tsp45I | GTSAC | 1 cut(s) | 96 |
| TspDTI | ATGAA | 4 cut(s) | 162, 219, 540, 584 |
| VneI | GTGCAC | 1 cut(s) | 16 |
| VpaK11BI | GGWCC | 1 cut(s) | 298 |
| XapI | RAATTY | 1 cut(s) | 236 |
| XhoI | CTCGAG | 1 cut(s) | 89 |
| XmaJI | CCTAGG | 1 cut(s) | 271 |
| XmiI | GTMKAC | 1 cut(s) | 152 |
| XmnI | GAANNNNTTC | 1 cut(s) | 236 |
| XspI | CTAG | 1 cut(s) | 272 |
| Zsp2I | ATGCAT | 1 cut(s) | 124 |