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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 2 cut(s) | 44, 266 |
| AciI | CCGC | 1 cut(s) | 134 |
| AclWI | GGATC | 2 cut(s) | 49, 131 |
| AfaI | GTAC | 1 cut(s) | 57 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 44, 72, 182, 266 |
| AgsI | TTSAA | 2 cut(s) | 154, 235 |
| AjnI | CCWGG | 1 cut(s) | 37 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 146, 178, 265, 297 |
| AluBI | AGCT | 2 cut(s) | 149, 333 |
| AluI | AGCT | 2 cut(s) | 149, 333 |
| AlwI | GGATC | 2 cut(s) | 49, 131 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 44 |
| ApeKI | GCWGC | 1 cut(s) | 137 |
| AseI | ATTAAT | 1 cut(s) | 224 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 239 |
| BbvI | GCAGC | 1 cut(s) | 124 |
| BciT130I | CCWGG | 1 cut(s) | 39 |
| BfaI | CTAG | 1 cut(s) | 334 |
| BfmI | CTRYAG | 1 cut(s) | 138 |
| BisI | GCNGC | 2 cut(s) | 135, 138 |
| BlsI | GCNGC | 2 cut(s) | 136, 139 |
| Bme1390I | CCNGG | 1 cut(s) | 39 |
| BmrFI | CCNGG | 1 cut(s) | 39 |
| BpmI | CTGGAG | 2 cut(s) | 159, 288 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 44, 72, 182, 266 |
| BseBI | CCWGG | 1 cut(s) | 39 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 44, 72, 182, 266 |
| BseMII | CTCAG | 1 cut(s) | 78 |
| BseXI | GCAGC | 1 cut(s) | 124 |
| BslFI | GGGAC | 2 cut(s) | 218, 299 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 44, 72, 182, 266 |
| BsmFI | GGGAC | 2 cut(s) | 218, 299 |
| Bsp143I | GATC | 2 cut(s) | 41, 123 |
| BspACI | CCGC | 1 cut(s) | 134 |
| BspCNI | CTCAG | 1 cut(s) | 79 |
| BspHI | TCATGA | 1 cut(s) | 93 |
| BspMAI | CTGCAG | 1 cut(s) | 142 |
| BspPI | GGATC | 2 cut(s) | 49, 131 |
| BssMI | GATC | 2 cut(s) | 41, 123 |
| Bst2UI | CCWGG | 1 cut(s) | 39 |
| Bst6I | CTCTTC | 2 cut(s) | 113, 300 |
| BstDEI | CTNAG | 1 cut(s) | 87 |
| BstKTI | GATC | 2 cut(s) | 44, 126 |
| BstMBI | GATC | 2 cut(s) | 41, 123 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 146 |
| BstNI | CCWGG | 1 cut(s) | 39 |
| BstSCI | CCNGG | 1 cut(s) | 37 |
| BstSFI | CTRYAG | 1 cut(s) | 138 |
| BstV1I | GCAGC | 1 cut(s) | 124 |
| BstX2I | RGATCY | 1 cut(s) | 41 |
| BstYI | RGATCY | 1 cut(s) | 41 |
| CaiI | CAGNNNCTG | 1 cut(s) | 44 |
| CciI | TCATGA | 1 cut(s) | 93 |
| Csp6I | GTAC | 1 cut(s) | 56 |
| CviAII | CATG | 1 cut(s) | 94 |
| CviJI | RGCY | 3 cut(s) | 137, 149, 333 |
| CviKI_1 | RGCY | 3 cut(s) | 137, 149, 333 |
| CviQI | GTAC | 1 cut(s) | 56 |
| DdeI | CTNAG | 1 cut(s) | 87 |
| DpnI | GATC | 2 cut(s) | 43, 125 |
| DpnII | GATC | 2 cut(s) | 41, 123 |
| Eam1104I | CTCTTC | 2 cut(s) | 113, 300 |
| EarI | CTCTTC | 2 cut(s) | 113, 300 |
| EcoRII | CCWGG | 1 cut(s) | 37 |
| FaeI | CATG | 1 cut(s) | 97 |
| FaiI | YATR | 7 cut(s) | 17, 23, 79, 95, 291, 293, 330 |
| FaqI | GGGAC | 2 cut(s) | 218, 299 |
| FatI | CATG | 1 cut(s) | 93 |
| Fnu4HI | GCNGC | 2 cut(s) | 135, 138 |
| Fsp4HI | GCNGC | 2 cut(s) | 135, 138 |
| FspBI | CTAG | 1 cut(s) | 334 |
| GluI | GCNGC | 2 cut(s) | 135, 138 |
| GsuI | CTGGAG | 2 cut(s) | 159, 288 |
| Hin1II | CATG | 1 cut(s) | 97 |
| HincII | GTYRAC | 1 cut(s) | 6 |
| HindII | GTYRAC | 1 cut(s) | 6 |
| HindIII | AAGCTT | 1 cut(s) | 147 |
| Hpy166II | GTNNAC | 1 cut(s) | 6 |
| Hpy188I | TCNGA | 1 cut(s) | 88 |
| Hpy188III | TCNNGA | 3 cut(s) | 94, 176, 267 |
| Hpy8I | GTNNAC | 1 cut(s) | 6 |
| HpyAV | CCTTC | 2 cut(s) | 65, 151 |
| HpyCH4V | TGCA | 1 cut(s) | 140 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 146 |
| HpyF3I | CTNAG | 1 cut(s) | 87 |
| Hsp92II | CATG | 1 cut(s) | 97 |
| Kzo9I | GATC | 2 cut(s) | 41, 123 |
| LpnPI | CCDG | 6 cut(s) | 24, 30, 51, 189, 252, 305 |
| Lsp1109I | GCAGC | 1 cut(s) | 124 |
| MaeI | CTAG | 1 cut(s) | 334 |
| MalI | GATC | 2 cut(s) | 43, 125 |
| MboI | GATC | 2 cut(s) | 41, 123 |
| MboII | GAAGA | 5 cut(s) | 130, 166, 200, 317, 336 |
| MflI | RGATCY | 1 cut(s) | 41 |
| MluCI | AATT | 1 cut(s) | 248 |
| MnlI | CCTC | 4 cut(s) | 114, 136, 263, 301 |
| MroXI | GAANNNNTTC | 1 cut(s) | 239 |
| MseI | TTAA | 1 cut(s) | 224 |
| MspR9I | CCNGG | 1 cut(s) | 39 |
| MvaI | CCWGG | 1 cut(s) | 39 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 146 |
| NdeII | GATC | 2 cut(s) | 41, 123 |
| NlaIII | CATG | 1 cut(s) | 97 |
| PagI | TCATGA | 1 cut(s) | 93 |
| PdmI | GAANNNNTTC | 1 cut(s) | 239 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 44, 266 |
| PkrI | GCNGC | 2 cut(s) | 136, 139 |
| PshBI | ATTAAT | 1 cut(s) | 224 |
| Psp6I | CCWGG | 1 cut(s) | 37 |
| PspGI | CCWGG | 1 cut(s) | 37 |
| PstI | CTGCAG | 1 cut(s) | 142 |
| PstNI | CAGNNNCTG | 1 cut(s) | 44 |
| PsuI | RGATCY | 1 cut(s) | 41 |
| RsaI | GTAC | 1 cut(s) | 57 |
| RsaNI | GTAC | 1 cut(s) | 56 |
| SaqAI | TTAA | 1 cut(s) | 224 |
| SatI | GCNGC | 2 cut(s) | 135, 138 |
| Sau3AI | GATC | 2 cut(s) | 41, 123 |
| ScrFI | CCNGG | 1 cut(s) | 39 |
| SetI | ASST | 4 cut(s) | 151, 240, 312, 335 |
| SfcI | CTRYAG | 1 cut(s) | 138 |
| Sse9I | AATT | 1 cut(s) | 248 |
| SsiI | CCGC | 1 cut(s) | 134 |
| SspMI | CTAG | 1 cut(s) | 334 |
| StyD4I | CCNGG | 1 cut(s) | 37 |
| TasI | AATT | 1 cut(s) | 248 |
| TauI | GCSGC | 1 cut(s) | 137 |
| Tru1I | TTAA | 1 cut(s) | 224 |
| Tru9I | TTAA | 1 cut(s) | 224 |
| TseI | GCWGC | 1 cut(s) | 137 |
| TspDTI | ATGAA | 2 cut(s) | 82, 232 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 44, 266 |
| VspI | ATTAAT | 1 cut(s) | 224 |
| XmnI | GAANNNNTTC | 1 cut(s) | 239 |
| XspI | CTAG | 1 cut(s) | 334 |