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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 38 |
| AclWI | GGATC | 1 cut(s) | 173 |
| AgsI | TTSAA | 2 cut(s) | 362, 374 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 257, 289 |
| AluBI | AGCT | 4 cut(s) | 35, 48, 95, 326 |
| AluI | AGCT | 4 cut(s) | 35, 48, 95, 326 |
| Alw26I | GTCTC | 1 cut(s) | 494 |
| AlwI | GGATC | 1 cut(s) | 173 |
| ApeKI | GCWGC | 4 cut(s) | 32, 40, 95, 107 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 369 |
| AsuHPI | GGTGA | 1 cut(s) | 479 |
| BanII | GRGCYC | 2 cut(s) | 11, 284 |
| BbsI | GAAGAC | 1 cut(s) | 413 |
| BbvI | GCAGC | 4 cut(s) | 44, 52, 82, 94 |
| BccI | CCATC | 1 cut(s) | 262 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 167, 201 |
| BcoDI | GTCTC | 1 cut(s) | 494 |
| BfmI | CTRYAG | 2 cut(s) | 186, 354 |
| BisI | GCNGC | 4 cut(s) | 33, 41, 96, 108 |
| BlsI | GCNGC | 4 cut(s) | 34, 42, 97, 109 |
| BmsI | GCATC | 2 cut(s) | 107, 209 |
| BoxI | GACNNNNGTC | 1 cut(s) | 333 |
| BpiI | GAAGAC | 1 cut(s) | 413 |
| BsaI | GGTCTC | 1 cut(s) | 494 |
| BsaJI | CCNNGG | 1 cut(s) | 160 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 19, 49, 257, 287 |
| BseDI | CCNNGG | 1 cut(s) | 160 |
| BseXI | GCAGC | 4 cut(s) | 44, 52, 82, 94 |
| BsgI | GTGCAG | 1 cut(s) | 205 |
| BslFI | GGGAC | 1 cut(s) | 142 |
| BsmAI | GTCTC | 1 cut(s) | 494 |
| BsmFI | GGGAC | 1 cut(s) | 142 |
| Bso31I | GGTCTC | 1 cut(s) | 494 |
| Bsp1286I | GDGCHC | 2 cut(s) | 11, 284 |
| Bsp143I | GATC | 2 cut(s) | 130, 165 |
| Bsp19I | CCATGG | 1 cut(s) | 160 |
| BspACI | CCGC | 1 cut(s) | 38 |
| BspMAI | CTGCAG | 1 cut(s) | 190 |
| BspPI | GGATC | 1 cut(s) | 173 |
| BspTNI | GGTCTC | 1 cut(s) | 494 |
| BssECI | CCNNGG | 1 cut(s) | 160 |
| BssMI | GATC | 2 cut(s) | 130, 165 |
| BssT1I | CCWWGG | 1 cut(s) | 160 |
| Bst4CI | ACNGT | 6 cut(s) | 142, 175, 226, 273, 304, 475 |
| BstDEI | CTNAG | 1 cut(s) | 102 |
| BstDSI | CCRYGG | 1 cut(s) | 160 |
| BstKTI | GATC | 2 cut(s) | 133, 168 |
| BstMAI | GTCTC | 1 cut(s) | 494 |
| BstMBI | GATC | 2 cut(s) | 130, 165 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 104 |
| BstNSI | RCATGY | 1 cut(s) | 298 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 333 |
| BstSFI | CTRYAG | 2 cut(s) | 186, 354 |
| BstV1I | GCAGC | 4 cut(s) | 44, 52, 82, 94 |
| BstV2I | GAAGAC | 1 cut(s) | 413 |
| BstX2I | RGATCY | 1 cut(s) | 165 |
| BstYI | RGATCY | 1 cut(s) | 165 |
| BtgI | CCRYGG | 1 cut(s) | 160 |
| BtsI | GCAGTG | 1 cut(s) | 217 |
| BtsIMutI | CAGTG | 3 cut(s) | 217, 231, 309 |
| CviAII | CATG | 2 cut(s) | 161, 295 |
| CviJI | RGCY | 8 cut(s) | 9, 35, 48, 95, 107, 282, 326, 423 |
| CviKI_1 | RGCY | 8 cut(s) | 9, 35, 48, 95, 107, 282, 326, 423 |
| DdeI | CTNAG | 1 cut(s) | 102 |
| DpnI | GATC | 2 cut(s) | 132, 167 |
| DpnII | GATC | 2 cut(s) | 130, 165 |
| Eco130I | CCWWGG | 1 cut(s) | 160 |
| Eco24I | GRGCYC | 2 cut(s) | 11, 284 |
| Eco31I | GGTCTC | 1 cut(s) | 494 |
| EcoT14I | CCWWGG | 1 cut(s) | 160 |
| EcoT38I | GRGCYC | 2 cut(s) | 11, 284 |
| ErhI | CCWWGG | 1 cut(s) | 160 |
| FaeI | CATG | 2 cut(s) | 164, 298 |
| FaiI | YATR | 5 cut(s) | 162, 261, 296, 356, 392 |
| FaqI | GGGAC | 1 cut(s) | 142 |
| FatI | CATG | 2 cut(s) | 160, 294 |
| Fnu4HI | GCNGC | 4 cut(s) | 33, 41, 96, 108 |
| FriOI | GRGCYC | 2 cut(s) | 11, 284 |
| Fsp4HI | GCNGC | 4 cut(s) | 33, 41, 96, 108 |
| GluI | GCNGC | 4 cut(s) | 33, 41, 96, 108 |
| Hin1II | CATG | 2 cut(s) | 164, 298 |
| HincII | GTYRAC | 1 cut(s) | 438 |
| HindII | GTYRAC | 1 cut(s) | 438 |
| HinfI | GANTC | 3 cut(s) | 16, 182, 266 |
| HpaI | GTTAAC | 1 cut(s) | 438 |
| HphI | GGTGA | 1 cut(s) | 479 |
| Hpy166II | GTNNAC | 1 cut(s) | 438 |
| Hpy188I | TCNGA | 1 cut(s) | 339 |
| Hpy188III | TCNNGA | 1 cut(s) | 277 |
| Hpy8I | GTNNAC | 1 cut(s) | 438 |
| HpyAV | CCTTC | 2 cut(s) | 65, 143 |
| HpyCH4III | ACNGT | 6 cut(s) | 142, 175, 226, 273, 304, 475 |
| HpyCH4IV | ACGT | 1 cut(s) | 501 |
| HpyCH4V | TGCA | 4 cut(s) | 98, 188, 222, 445 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 104 |
| HpyF3I | CTNAG | 1 cut(s) | 102 |
| HpySE526I | ACGT | 1 cut(s) | 501 |
| Hsp92II | CATG | 2 cut(s) | 164, 298 |
| KspAI | GTTAAC | 1 cut(s) | 438 |
| Kzo9I | GATC | 2 cut(s) | 130, 165 |
| LmnI | GCTCC | 2 cut(s) | 40, 53 |
| LpnPI | CCDG | 3 cut(s) | 290, 426, 450 |
| Lsp1109I | GCAGC | 4 cut(s) | 44, 52, 82, 94 |
| LweI | GCATC | 2 cut(s) | 107, 209 |
| MaeII | ACGT | 1 cut(s) | 501 |
| MaeIII | GTNAC | 1 cut(s) | 267 |
| MalI | GATC | 2 cut(s) | 132, 167 |
| MboI | GATC | 2 cut(s) | 130, 165 |
| MboII | GAAGA | 6 cut(s) | 191, 245, 374, 415, 418, 537 |
| MflI | RGATCY | 1 cut(s) | 165 |
| MhlI | GDGCHC | 2 cut(s) | 11, 284 |
| MluCI | AATT | 1 cut(s) | 83 |
| MlyI | GAGTC | 2 cut(s) | 10, 275 |
| MnlI | CCTC | 4 cut(s) | 34, 66, 139, 225 |
| MroXI | GAANNNNTTC | 1 cut(s) | 369 |
| MseI | TTAA | 3 cut(s) | 86, 437, 512 |
| MslI | CAYNNNNRTG | 1 cut(s) | 465 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 104 |
| NcoI | CCATGG | 1 cut(s) | 160 |
| NdeII | GATC | 2 cut(s) | 130, 165 |
| NlaIII | CATG | 2 cut(s) | 164, 298 |
| NmuCI | GTSAC | 1 cut(s) | 267 |
| NspI | RCATGY | 1 cut(s) | 298 |
| PdmI | GAANNNNTTC | 1 cut(s) | 369 |
| PfeI | GAWTC | 1 cut(s) | 182 |
| PkrI | GCNGC | 4 cut(s) | 34, 42, 97, 109 |
| PleI | GAGTC | 2 cut(s) | 10, 274 |
| PpsI | GAGTC | 2 cut(s) | 10, 274 |
| PshAI | GACNNNNGTC | 1 cut(s) | 333 |
| PstI | CTGCAG | 1 cut(s) | 190 |
| PsuI | RGATCY | 1 cut(s) | 165 |
| RseI | CAYNNNNRTG | 1 cut(s) | 465 |
| SaqAI | TTAA | 3 cut(s) | 86, 437, 512 |
| SatI | GCNGC | 4 cut(s) | 33, 41, 96, 108 |
| Sau3AI | GATC | 2 cut(s) | 130, 165 |
| SchI | GAGTC | 2 cut(s) | 10, 275 |
| SduI | GDGCHC | 2 cut(s) | 11, 284 |
| SfaNI | GCATC | 2 cut(s) | 107, 209 |
| SfcI | CTRYAG | 2 cut(s) | 186, 354 |
| SgeI | CNNG | 7 cut(s) | 173, 289, 307, 335, 343, 425, 477 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 465 |
| Sse9I | AATT | 1 cut(s) | 83 |
| SsiI | CCGC | 1 cut(s) | 38 |
| StyI | CCWWGG | 1 cut(s) | 160 |
| TaaI | ACNGT | 6 cut(s) | 142, 175, 226, 273, 304, 475 |
| TaiI | ACGT | 1 cut(s) | 504 |
| TaqI | TCGA | 3 cut(s) | 137, 177, 245 |
| TasI | AATT | 1 cut(s) | 83 |
| TfiI | GAWTC | 1 cut(s) | 182 |
| Tru1I | TTAA | 3 cut(s) | 86, 437, 512 |
| Tru9I | TTAA | 3 cut(s) | 86, 437, 512 |
| TscAI | CASTG | 3 cut(s) | 224, 231, 309 |
| TseFI | GTSAC | 1 cut(s) | 267 |
| TseI | GCWGC | 4 cut(s) | 32, 40, 95, 107 |
| Tsp45I | GTSAC | 1 cut(s) | 267 |
| TspDTI | ATGAA | 2 cut(s) | 96, 105 |
| TspRI | CASTG | 3 cut(s) | 224, 231, 309 |
| XceI | RCATGY | 1 cut(s) | 298 |
| XmnI | GAANNNNTTC | 1 cut(s) | 369 |