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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 368 |
| AciI | CCGC | 3 cut(s) | 160, 370, 513 |
| AclWI | GGATC | 1 cut(s) | 461 |
| AcsI | RAATTY | 1 cut(s) | 325 |
| AfaI | GTAC | 1 cut(s) | 388 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 403, 593 |
| AgsI | TTSAA | 3 cut(s) | 228, 484, 563 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 476, 508, 592, 624 |
| AluBI | AGCT | 2 cut(s) | 173, 479 |
| AluI | AGCT | 2 cut(s) | 173, 479 |
| AlwI | GGATC | 1 cut(s) | 461 |
| Ama87I | CYCGRG | 1 cut(s) | 121 |
| ApeKI | GCWGC | 2 cut(s) | 173, 176 |
| ApoI | RAATTY | 1 cut(s) | 325 |
| AseI | ATTAAT | 1 cut(s) | 552 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 567 |
| AspS9I | GGNCC | 1 cut(s) | 300 |
| AsuC2I | CCSGG | 2 cut(s) | 122, 123 |
| AsuHPI | GGTGA | 1 cut(s) | 356 |
| AvaI | CYCGRG | 1 cut(s) | 121 |
| AvaII | GGWCC | 1 cut(s) | 300 |
| BauI | CACGAG | 1 cut(s) | 628 |
| BbvI | GCAGC | 2 cut(s) | 160, 163 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 397, 431 |
| BcnI | CCSGG | 2 cut(s) | 122, 123 |
| BfaI | CTAG | 1 cut(s) | 14 |
| BfuAI | ACCTGC | 1 cut(s) | 368 |
| BisI | GCNGC | 2 cut(s) | 174, 177 |
| BlsI | GCNGC | 2 cut(s) | 175, 178 |
| Bme1390I | CCNGG | 2 cut(s) | 122, 123 |
| Bme18I | GGWCC | 1 cut(s) | 300 |
| BmeT110I | CYCGRG | 1 cut(s) | 121 |
| BmgT120I | GGNCC | 1 cut(s) | 300 |
| BmrFI | CCNGG | 2 cut(s) | 122, 123 |
| BmsI | GCATC | 3 cut(s) | 199, 232, 364 |
| BpuMI | CCSGG | 2 cut(s) | 122, 123 |
| BsaJI | CCNNGG | 3 cut(s) | 121, 122, 217 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 403, 593 |
| BseDI | CCNNGG | 3 cut(s) | 121, 122, 217 |
| BseGI | GGATG | 3 cut(s) | 106, 247, 379 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 403, 593 |
| BseXI | GCAGC | 2 cut(s) | 160, 163 |
| BsgI | GTGCAG | 1 cut(s) | 489 |
| BsiHKCI | CYCGRG | 1 cut(s) | 121 |
| BsiSI | CCGG | 1 cut(s) | 122 |
| BslFI | GGGAC | 4 cut(s) | 104, 176, 546, 626 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 403, 593 |
| BsmFI | GGGAC | 4 cut(s) | 104, 176, 546, 626 |
| BsmI | GAATGC | 1 cut(s) | 212 |
| BsoBI | CYCGRG | 1 cut(s) | 121 |
| Bsp143I | GATC | 1 cut(s) | 453 |
| Bsp19I | CCATGG | 1 cut(s) | 217 |
| BspACI | CCGC | 3 cut(s) | 160, 370, 513 |
| BspMI | ACCTGC | 1 cut(s) | 368 |
| BspPI | GGATC | 1 cut(s) | 461 |
| BssECI | CCNNGG | 3 cut(s) | 121, 122, 217 |
| BssMI | GATC | 1 cut(s) | 453 |
| BssSI | CACGAG | 1 cut(s) | 628 |
| BssT1I | CCWWGG | 1 cut(s) | 217 |
| Bst2BI | CACGAG | 1 cut(s) | 628 |
| Bst4CI | ACNGT | 1 cut(s) | 414 |
| Bst6I | CTCTTC | 2 cut(s) | 102, 443 |
| BstC8I | GCNNGC | 1 cut(s) | 477 |
| BstDSI | CCRYGG | 1 cut(s) | 217 |
| BstF5I | GGATG | 3 cut(s) | 106, 247, 379 |
| BstKTI | GATC | 1 cut(s) | 456 |
| BstMBI | GATC | 1 cut(s) | 453 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 476 |
| BstSCI | CCNGG | 2 cut(s) | 120, 121 |
| BstV1I | GCAGC | 2 cut(s) | 160, 163 |
| BtgI | CCRYGG | 1 cut(s) | 217 |
| BtsCI | GGATG | 3 cut(s) | 106, 247, 379 |
| BveI | ACCTGC | 1 cut(s) | 368 |
| Cac8I | GCNNGC | 1 cut(s) | 477 |
| Cfr13I | GGNCC | 1 cut(s) | 300 |
| Cfr9I | CCCGGG | 1 cut(s) | 121 |
| Csp6I | GTAC | 1 cut(s) | 387 |
| CviAII | CATG | 2 cut(s) | 218, 292 |
| CviJI | RGCY | 2 cut(s) | 173, 479 |
| CviKI_1 | RGCY | 2 cut(s) | 173, 479 |
| CviQI | GTAC | 1 cut(s) | 387 |
| DpnI | GATC | 1 cut(s) | 455 |
| DpnII | GATC | 1 cut(s) | 453 |
| Eam1104I | CTCTTC | 2 cut(s) | 102, 443 |
| EarI | CTCTTC | 2 cut(s) | 102, 443 |
| Eco130I | CCWWGG | 1 cut(s) | 217 |
| Eco47I | GGWCC | 1 cut(s) | 300 |
| Eco88I | CYCGRG | 1 cut(s) | 121 |
| EcoT14I | CCWWGG | 1 cut(s) | 217 |
| EcoT22I | ATGCAT | 1 cut(s) | 214 |
| ErhI | CCWWGG | 1 cut(s) | 217 |
| FaeI | CATG | 2 cut(s) | 221, 295 |
| FaqI | GGGAC | 4 cut(s) | 104, 176, 546, 626 |
| FatI | CATG | 2 cut(s) | 217, 291 |
| FauI | CCCGC | 2 cut(s) | 153, 363 |
| Fnu4HI | GCNGC | 2 cut(s) | 174, 177 |
| FokI | GGATG | 3 cut(s) | 113, 254, 386 |
| Fsp4HI | GCNGC | 2 cut(s) | 174, 177 |
| FspBI | CTAG | 1 cut(s) | 14 |
| GluI | GCNGC | 2 cut(s) | 174, 177 |
| HapII | CCGG | 1 cut(s) | 122 |
| Hin1II | CATG | 2 cut(s) | 221, 295 |
| HincII | GTYRAC | 1 cut(s) | 338 |
| HindII | GTYRAC | 1 cut(s) | 338 |
| HindIII | AAGCTT | 1 cut(s) | 477 |
| HinfI | GANTC | 2 cut(s) | 10, 624 |
| HpaII | CCGG | 1 cut(s) | 122 |
| HphI | GGTGA | 1 cut(s) | 356 |
| Hpy166II | GTNNAC | 1 cut(s) | 338 |
| Hpy188I | TCNGA | 4 cut(s) | 256, 331, 506, 596 |
| Hpy188III | TCNNGA | 3 cut(s) | 424, 560, 630 |
| Hpy8I | GTNNAC | 1 cut(s) | 338 |
| HpyAV | CCTTC | 3 cut(s) | 61, 396, 481 |
| HpyCH4III | ACNGT | 1 cut(s) | 414 |
| HpyCH4V | TGCA | 6 cut(s) | 179, 197, 212, 245, 377, 470 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 476 |
| Hsp92II | CATG | 2 cut(s) | 221, 295 |
| Kzo9I | GATC | 1 cut(s) | 453 |
| LpnPI | CCDG | 4 cut(s) | 135, 306, 363, 594 |
| Lsp1109I | GCAGC | 2 cut(s) | 160, 163 |
| LweI | GCATC | 3 cut(s) | 199, 232, 364 |
| MaeI | CTAG | 1 cut(s) | 14 |
| MalI | GATC | 1 cut(s) | 455 |
| MboI | GATC | 1 cut(s) | 453 |
| MluCI | AATT | 2 cut(s) | 228, 325 |
| MlyI | GAGTC | 1 cut(s) | 633 |
| MnlI | CCTC | 4 cut(s) | 103, 142, 444, 590 |
| Mph1103I | ATGCAT | 1 cut(s) | 214 |
| MroXI | GAANNNNTTC | 1 cut(s) | 567 |
| MseI | TTAA | 3 cut(s) | 231, 263, 552 |
| MspA1I | CMGCKG | 1 cut(s) | 513 |
| MspI | CCGG | 1 cut(s) | 122 |
| MspR9I | CCNGG | 2 cut(s) | 122, 123 |
| Mva1269I | GAATGC | 1 cut(s) | 212 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 476 |
| NciI | CCSGG | 2 cut(s) | 122, 123 |
| NcoI | CCATGG | 1 cut(s) | 217 |
| NdeII | GATC | 1 cut(s) | 453 |
| NlaIII | CATG | 2 cut(s) | 221, 295 |
| NsiI | ATGCAT | 1 cut(s) | 214 |
| PctI | GAATGC | 1 cut(s) | 212 |
| PdmI | GAANNNNTTC | 1 cut(s) | 567 |
| PfeI | GAWTC | 1 cut(s) | 10 |
| PkrI | GCNGC | 2 cut(s) | 175, 178 |
| PleI | GAGTC | 1 cut(s) | 632 |
| PpsI | GAGTC | 1 cut(s) | 632 |
| PshBI | ATTAAT | 1 cut(s) | 552 |
| PspPI | GGNCC | 1 cut(s) | 300 |
| RsaI | GTAC | 1 cut(s) | 388 |
| RsaNI | GTAC | 1 cut(s) | 387 |
| SaqAI | TTAA | 3 cut(s) | 231, 263, 552 |
| SatI | GCNGC | 2 cut(s) | 174, 177 |
| Sau3AI | GATC | 1 cut(s) | 453 |
| Sau96I | GGNCC | 1 cut(s) | 300 |
| SchI | GAGTC | 1 cut(s) | 633 |
| ScrFI | CCNGG | 2 cut(s) | 122, 123 |
| SetI | ASST | 6 cut(s) | 175, 305, 346, 382, 465, 481 |
| SfaNI | GCATC | 3 cut(s) | 199, 232, 364 |
| SinI | GGWCC | 1 cut(s) | 300 |
| SmaI | CCCGGG | 1 cut(s) | 123 |
| Sse9I | AATT | 2 cut(s) | 228, 325 |
| SsiI | CCGC | 3 cut(s) | 160, 370, 513 |
| SspMI | CTAG | 1 cut(s) | 14 |
| StyD4I | CCNGG | 2 cut(s) | 120, 121 |
| StyI | CCWWGG | 1 cut(s) | 217 |
| TaaI | ACNGT | 1 cut(s) | 414 |
| TaqI | TCGA | 1 cut(s) | 417 |
| TasI | AATT | 2 cut(s) | 228, 325 |
| TfiI | GAWTC | 1 cut(s) | 10 |
| Tru1I | TTAA | 3 cut(s) | 231, 263, 552 |
| Tru9I | TTAA | 3 cut(s) | 231, 263, 552 |
| TseI | GCWGC | 2 cut(s) | 173, 176 |
| TspDTI | ATGAA | 3 cut(s) | 17, 120, 560 |
| TspGWI | ACGGA | 1 cut(s) | 313 |
| TspMI | CCCGGG | 1 cut(s) | 121 |
| VpaK11BI | GGWCC | 1 cut(s) | 300 |
| VspI | ATTAAT | 1 cut(s) | 552 |
| XapI | RAATTY | 1 cut(s) | 325 |
| XmaI | CCCGGG | 1 cut(s) | 121 |
| XmnI | GAANNNNTTC | 1 cut(s) | 567 |
| XspI | CTAG | 1 cut(s) | 14 |
| Zsp2I | ATGCAT | 1 cut(s) | 214 |