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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 759 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 357 |
| AciI | CCGC | 1 cut(s) | 348 |
| AcsI | RAATTY | 2 cut(s) | 218, 729 |
| AfaI | GTAC | 2 cut(s) | 281, 584 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 53, 269, 296, 335, 357 |
| AgsI | TTSAA | 3 cut(s) | 120, 430, 448 |
| AjiI | CACGTC | 1 cut(s) | 602 |
| AjnI | CCWGG | 1 cut(s) | 604 |
| AluBI | AGCT | 2 cut(s) | 67, 176 |
| AluI | AGCT | 2 cut(s) | 67, 176 |
| Alw26I | GTCTC | 1 cut(s) | 594 |
| Ama87I | CYCGRG | 1 cut(s) | 675 |
| AoxI | GGCC | 1 cut(s) | 669 |
| ApeKI | GCWGC | 2 cut(s) | 67, 70 |
| ApoI | RAATTY | 2 cut(s) | 218, 729 |
| AspLEI | GCGC | 1 cut(s) | 771 |
| AspS9I | GGNCC | 2 cut(s) | 193, 670 |
| AsuC2I | CCSGG | 1 cut(s) | 271 |
| AsuHPI | GGTGA | 3 cut(s) | 519, 620, 814 |
| AvaI | CYCGRG | 1 cut(s) | 675 |
| AvaII | GGWCC | 1 cut(s) | 193 |
| BbvI | GCAGC | 2 cut(s) | 54, 57 |
| BccI | CCATC | 1 cut(s) | 559 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 126, 160 |
| BciT130I | CCWGG | 1 cut(s) | 606 |
| BcnI | CCSGG | 1 cut(s) | 271 |
| BcoDI | GTCTC | 1 cut(s) | 594 |
| BfaI | CTAG | 1 cut(s) | 645 |
| BfmI | CTRYAG | 2 cut(s) | 71, 787 |
| BfuAI | ACCTGC | 1 cut(s) | 759 |
| BglII | AGATCT | 1 cut(s) | 307 |
| BisI | GCNGC | 3 cut(s) | 68, 71, 348 |
| BlsI | GCNGC | 3 cut(s) | 69, 72, 349 |
| Bme1390I | CCNGG | 2 cut(s) | 271, 606 |
| Bme18I | GGWCC | 1 cut(s) | 193 |
| BmeT110I | CYCGRG | 1 cut(s) | 675 |
| BmgBI | CACGTC | 1 cut(s) | 602 |
| BmgT120I | GGNCC | 2 cut(s) | 193, 670 |
| BmiI | GGNNCC | 1 cut(s) | 672 |
| BmrFI | CCNGG | 2 cut(s) | 271, 606 |
| BmsI | GCATC | 3 cut(s) | 91, 124, 780 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 509, 541 |
| BpuEI | CTTGAG | 1 cut(s) | 705 |
| BpuMI | CCSGG | 1 cut(s) | 271 |
| BsaJI | CCNNGG | 4 cut(s) | 109, 435, 674, 809 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 616, 646 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 53, 269, 296, 335, 357 |
| BseBI | CCWGG | 1 cut(s) | 606 |
| BseDI | CCNNGG | 4 cut(s) | 109, 435, 674, 809 |
| BseGI | GGATG | 4 cut(s) | 139, 154, 487, 771 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 53, 269, 296, 335, 357 |
| BseMII | CTCAG | 1 cut(s) | 302 |
| BseRI | GAGGAG | 2 cut(s) | 618, 621 |
| BseXI | GCAGC | 2 cut(s) | 54, 57 |
| Bsh1285I | CGRYCG | 1 cut(s) | 51 |
| BshFI | GGCC | 1 cut(s) | 671 |
| BsiEI | CGRYCG | 1 cut(s) | 51 |
| BsiHKCI | CYCGRG | 1 cut(s) | 675 |
| BsiSI | CCGG | 3 cut(s) | 263, 270, 726 |
| BslFI | GGGAC | 1 cut(s) | 70 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 53, 269, 296, 335, 357 |
| BsmAI | GTCTC | 1 cut(s) | 594 |
| BsmFI | GGGAC | 1 cut(s) | 70 |
| BsmI | GAATGC | 1 cut(s) | 104 |
| BsnI | GGCC | 1 cut(s) | 671 |
| BsoBI | CYCGRG | 1 cut(s) | 675 |
| Bsp143I | GATC | 2 cut(s) | 48, 307 |
| Bsp19I | CCATGG | 2 cut(s) | 109, 435 |
| BspACI | CCGC | 1 cut(s) | 348 |
| BspANI | GGCC | 1 cut(s) | 671 |
| BspCNI | CTCAG | 1 cut(s) | 303 |
| BspLI | GGNNCC | 1 cut(s) | 672 |
| BspMAI | CTGCAG | 2 cut(s) | 75, 791 |
| BspMI | ACCTGC | 1 cut(s) | 759 |
| BssECI | CCNNGG | 4 cut(s) | 109, 435, 674, 809 |
| BssMI | GATC | 2 cut(s) | 48, 307 |
| BssT1I | CCWWGG | 2 cut(s) | 109, 435 |
| Bst2UI | CCWGG | 1 cut(s) | 606 |
| Bst4CI | ACNGT | 1 cut(s) | 163 |
| Bst6I | CTCTTC | 3 cut(s) | 356, 443, 468 |
| BstC8I | GCNNGC | 3 cut(s) | 6, 698, 767 |
| BstDEI | CTNAG | 1 cut(s) | 311 |
| BstDSI | CCRYGG | 2 cut(s) | 109, 435 |
| BstEII | GGTNACC | 1 cut(s) | 820 |
| BstF5I | GGATG | 4 cut(s) | 139, 154, 487, 771 |
| BstHHI | GCGC | 1 cut(s) | 771 |
| BstKTI | GATC | 2 cut(s) | 51, 310 |
| BstMAI | GTCTC | 1 cut(s) | 594 |
| BstMBI | GATC | 2 cut(s) | 48, 307 |
| BstMCI | CGRYCG | 1 cut(s) | 51 |
| BstNI | CCWGG | 1 cut(s) | 606 |
| BstPI | GGTNACC | 1 cut(s) | 820 |
| BstSCI | CCNGG | 2 cut(s) | 269, 604 |
| BstSFI | CTRYAG | 2 cut(s) | 71, 787 |
| BstV1I | GCAGC | 2 cut(s) | 54, 57 |
| BstX2I | RGATCY | 1 cut(s) | 307 |
| BstYI | RGATCY | 1 cut(s) | 307 |
| BsuRI | GGCC | 1 cut(s) | 671 |
| BtgI | CCRYGG | 2 cut(s) | 109, 435 |
| BtrI | CACGTC | 1 cut(s) | 602 |
| BtsCI | GGATG | 4 cut(s) | 139, 154, 487, 771 |
| BveI | ACCTGC | 1 cut(s) | 759 |
| Cac8I | GCNNGC | 3 cut(s) | 6, 698, 767 |
| CfoI | GCGC | 1 cut(s) | 771 |
| Cfr13I | GGNCC | 2 cut(s) | 193, 670 |
| Csp6I | GTAC | 2 cut(s) | 280, 583 |
| CviAII | CATG | 2 cut(s) | 110, 436 |
| CviJI | RGCY | 6 cut(s) | 67, 176, 524, 671, 685, 725 |
| CviKI_1 | RGCY | 6 cut(s) | 67, 176, 524, 671, 685, 725 |
| CviQI | GTAC | 2 cut(s) | 280, 583 |
| DdeI | CTNAG | 1 cut(s) | 311 |
| DpnI | GATC | 2 cut(s) | 50, 309 |
| DpnII | GATC | 2 cut(s) | 48, 307 |
| Eam1104I | CTCTTC | 3 cut(s) | 356, 443, 468 |
| EarI | CTCTTC | 3 cut(s) | 356, 443, 468 |
| Eco130I | CCWWGG | 2 cut(s) | 109, 435 |
| Eco32I | GATATC | 1 cut(s) | 816 |
| Eco47I | GGWCC | 1 cut(s) | 193 |
| Eco88I | CYCGRG | 1 cut(s) | 675 |
| Eco91I | GGTNACC | 1 cut(s) | 820 |
| EcoO109I | RGGNCCY | 1 cut(s) | 670 |
| EcoO65I | GGTNACC | 1 cut(s) | 820 |
| EcoRI | GAATTC | 1 cut(s) | 729 |
| EcoRII | CCWGG | 1 cut(s) | 604 |
| EcoRV | GATATC | 1 cut(s) | 816 |
| EcoT14I | CCWWGG | 2 cut(s) | 109, 435 |
| EcoT22I | ATGCAT | 1 cut(s) | 106 |
| ErhI | CCWWGG | 2 cut(s) | 109, 435 |
| FaeI | CATG | 2 cut(s) | 113, 439 |
| FaiI | YATR | 6 cut(s) | 111, 242, 248, 303, 437, 560 |
| FaqI | GGGAC | 1 cut(s) | 70 |
| FatI | CATG | 2 cut(s) | 109, 435 |
| Fnu4HI | GCNGC | 3 cut(s) | 68, 71, 348 |
| FokI | GGATG | 4 cut(s) | 146, 161, 494, 758 |
| Fsp4HI | GCNGC | 3 cut(s) | 68, 71, 348 |
| FspBI | CTAG | 1 cut(s) | 645 |
| GlaI | GCGC | 1 cut(s) | 770 |
| GluI | GCNGC | 3 cut(s) | 68, 71, 348 |
| HaeIII | GGCC | 1 cut(s) | 671 |
| HapII | CCGG | 3 cut(s) | 263, 270, 726 |
| HhaI | GCGC | 1 cut(s) | 771 |
| Hin1II | CATG | 2 cut(s) | 113, 439 |
| Hin6I | GCGC | 1 cut(s) | 769 |
| HinP1I | GCGC | 1 cut(s) | 769 |
| HindIII | AAGCTT | 1 cut(s) | 174 |
| HinfI | GANTC | 3 cut(s) | 266, 587, 798 |
| HpaII | CCGG | 3 cut(s) | 263, 270, 726 |
| HphI | GGTGA | 3 cut(s) | 519, 620, 814 |
| Hpy188I | TCNGA | 6 cut(s) | 48, 148, 224, 312, 735, 776 |
| Hpy188III | TCNNGA | 3 cut(s) | 14, 358, 677 |
| HpyAV | CCTTC | 3 cut(s) | 47, 289, 472 |
| HpyCH4III | ACNGT | 1 cut(s) | 163 |
| HpyCH4IV | ACGT | 1 cut(s) | 601 |
| HpyCH4V | TGCA | 5 cut(s) | 73, 89, 104, 137, 789 |
| HpyF3I | CTNAG | 1 cut(s) | 311 |
| HpySE526I | ACGT | 1 cut(s) | 601 |
| Hsp92II | CATG | 2 cut(s) | 113, 439 |
| HspAI | GCGC | 1 cut(s) | 769 |
| Kzo9I | GATC | 2 cut(s) | 48, 307 |
| LpnPI | CCDG | 8 cut(s) | 199, 276, 283, 343, 591, 618, 739, 764 |
| Lsp1109I | GCAGC | 2 cut(s) | 54, 57 |
| LweI | GCATC | 3 cut(s) | 91, 124, 780 |
| MaeI | CTAG | 1 cut(s) | 645 |
| MaeII | ACGT | 1 cut(s) | 601 |
| MaeIII | GTNAC | 1 cut(s) | 820 |
| MalI | GATC | 2 cut(s) | 50, 309 |
| MboI | GATC | 2 cut(s) | 48, 307 |
| MboII | GAAGA | 3 cut(s) | 373, 460, 485 |
| MfeI | CAATTG | 2 cut(s) | 22, 548 |
| MflI | RGATCY | 1 cut(s) | 307 |
| MluCI | AATT | 7 cut(s) | 22, 120, 218, 396, 457, 548, 729 |
| MlyI | GAGTC | 2 cut(s) | 596, 792 |
| Mph1103I | ATGCAT | 1 cut(s) | 106 |
| MseI | TTAA | 5 cut(s) | 123, 155, 399, 491, 534 |
| MslI | CAYNNNNRTG | 1 cut(s) | 563 |
| MspI | CCGG | 3 cut(s) | 263, 270, 726 |
| MspR9I | CCNGG | 2 cut(s) | 271, 606 |
| MunI | CAATTG | 2 cut(s) | 22, 548 |
| Mva1269I | GAATGC | 1 cut(s) | 104 |
| MvaI | CCWGG | 1 cut(s) | 606 |
| NciI | CCSGG | 1 cut(s) | 271 |
| NcoI | CCATGG | 2 cut(s) | 109, 435 |
| NdeII | GATC | 2 cut(s) | 48, 307 |
| NlaIII | CATG | 2 cut(s) | 113, 439 |
| NlaIV | GGNNCC | 1 cut(s) | 672 |
| NmuCI | GTSAC | 1 cut(s) | 820 |
| NsiI | ATGCAT | 1 cut(s) | 106 |
| PctI | GAATGC | 1 cut(s) | 104 |
| PfeI | GAWTC | 1 cut(s) | 266 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 357 |
| PkrI | GCNGC | 3 cut(s) | 69, 72, 349 |
| Ple19I | CGATCG | 1 cut(s) | 51 |
| PleI | GAGTC | 2 cut(s) | 595, 792 |
| PpsI | GAGTC | 2 cut(s) | 595, 792 |
| Psp6I | CCWGG | 1 cut(s) | 604 |
| PspEI | GGTNACC | 1 cut(s) | 820 |
| PspGI | CCWGG | 1 cut(s) | 604 |
| PspN4I | GGNNCC | 1 cut(s) | 672 |
| PspPI | GGNCC | 2 cut(s) | 193, 670 |
| PstI | CTGCAG | 2 cut(s) | 75, 791 |
| PsuI | RGATCY | 1 cut(s) | 307 |
| PvuI | CGATCG | 1 cut(s) | 51 |
| RsaI | GTAC | 2 cut(s) | 281, 584 |
| RsaNI | GTAC | 2 cut(s) | 280, 583 |
| RseI | CAYNNNNRTG | 1 cut(s) | 563 |
| SaqAI | TTAA | 5 cut(s) | 123, 155, 399, 491, 534 |
| SatI | GCNGC | 3 cut(s) | 68, 71, 348 |
| Sau3AI | GATC | 2 cut(s) | 48, 307 |
| Sau96I | GGNCC | 2 cut(s) | 193, 670 |
| SchI | GAGTC | 2 cut(s) | 596, 792 |
| ScrFI | CCNGG | 2 cut(s) | 271, 606 |
| SetI | ASST | 9 cut(s) | 69, 118, 178, 198, 507, 604, 610, 646, 753 |
| SfaNI | GCATC | 3 cut(s) | 91, 124, 780 |
| SfcI | CTRYAG | 2 cut(s) | 71, 787 |
| SinI | GGWCC | 1 cut(s) | 193 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 563 |
| SmlI | CTYRAG | 1 cut(s) | 720 |
| SmoI | CTYRAG | 1 cut(s) | 720 |
| Sse9I | AATT | 7 cut(s) | 22, 120, 218, 396, 457, 548, 729 |
| SsiI | CCGC | 1 cut(s) | 348 |
| SspMI | CTAG | 1 cut(s) | 645 |
| StyD4I | CCNGG | 2 cut(s) | 269, 604 |
| StyI | CCWWGG | 2 cut(s) | 109, 435 |
| TaaI | ACNGT | 1 cut(s) | 163 |
| TaiI | ACGT | 1 cut(s) | 604 |
| TaqI | TCGA | 2 cut(s) | 15, 51 |
| TaqII | GACCGA | 1 cut(s) | 808 |
| TasI | AATT | 7 cut(s) | 22, 120, 218, 396, 457, 548, 729 |
| TauI | GCSGC | 1 cut(s) | 350 |
| TfiI | GAWTC | 1 cut(s) | 266 |
| Tru1I | TTAA | 5 cut(s) | 123, 155, 399, 491, 534 |
| Tru9I | TTAA | 5 cut(s) | 123, 155, 399, 491, 534 |
| TseFI | GTSAC | 1 cut(s) | 820 |
| TseI | GCWGC | 2 cut(s) | 67, 70 |
| Tsp45I | GTSAC | 1 cut(s) | 820 |
| TspDTI | ATGAA | 1 cut(s) | 306 |
| TspGWI | ACGGA | 2 cut(s) | 206, 394 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 357 |
| VpaK11BI | GGWCC | 1 cut(s) | 193 |
| XapI | RAATTY | 2 cut(s) | 218, 729 |
| XspI | CTAG | 1 cut(s) | 645 |
| Zsp2I | ATGCAT | 1 cut(s) | 106 |