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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 3 cut(s) | 516, 626, 1064 |
| AclWI | GGATC | 1 cut(s) | 1074 |
| AcsI | RAATTY | 2 cut(s) | 455, 585 |
| AcuI | CTGAAG | 1 cut(s) | 59 |
| AfaI | GTAC | 3 cut(s) | 793, 1010, 1052 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 688, 1060 |
| AgsI | TTSAA | 4 cut(s) | 64, 173, 560, 980 |
| AjiI | CACGTC | 1 cut(s) | 329 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 78, 110 |
| AluBI | AGCT | 4 cut(s) | 31, 451, 578, 1000 |
| AluI | AGCT | 4 cut(s) | 31, 451, 578, 1000 |
| Alw26I | GTCTC | 3 cut(s) | 189, 321, 886 |
| AlwI | GGATC | 1 cut(s) | 1074 |
| Ama87I | CYCGRG | 2 cut(s) | 401, 605 |
| AoxI | GGCC | 1 cut(s) | 395 |
| ApeKI | GCWGC | 2 cut(s) | 578, 905 |
| ApoI | RAATTY | 2 cut(s) | 455, 585 |
| AseI | ATTAAT | 1 cut(s) | 939 |
| AspLEI | GCGC | 1 cut(s) | 500 |
| AspS9I | GGNCC | 1 cut(s) | 396 |
| AsuHPI | GGTGA | 5 cut(s) | 346, 543, 585, 757, 838 |
| AvaI | CYCGRG | 2 cut(s) | 401, 605 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 733, 766 |
| BbsI | GAAGAC | 1 cut(s) | 777 |
| BbvI | GCAGC | 2 cut(s) | 565, 917 |
| BccI | CCATC | 2 cut(s) | 109, 286 |
| BceAI | ACGGC | 1 cut(s) | 507 |
| BcoDI | GTCTC | 3 cut(s) | 189, 321, 886 |
| BfaI | CTAG | 1 cut(s) | 371 |
| BisI | GCNGC | 2 cut(s) | 579, 906 |
| BlsI | GCNGC | 2 cut(s) | 580, 907 |
| BmeT110I | CYCGRG | 2 cut(s) | 401, 605 |
| BmgBI | CACGTC | 1 cut(s) | 329 |
| BmgT120I | GGNCC | 1 cut(s) | 396 |
| BmiI | GGNNCC | 1 cut(s) | 398 |
| BmsI | GCATC | 2 cut(s) | 509, 622 |
| BoxI | GACNNNNGTC | 1 cut(s) | 547 |
| BpiI | GAAGAC | 1 cut(s) | 777 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 146, 178 |
| BpmI | CTGGAG | 1 cut(s) | 53 |
| BpuEI | CTTGAG | 1 cut(s) | 431 |
| Bsa29I | ATCGAT | 1 cut(s) | 753 |
| BsaI | GGTCTC | 1 cut(s) | 886 |
| BsaJI | CCNNGG | 5 cut(s) | 400, 489, 538, 606, 909 |
| BsaWI | WCCGGW | 2 cut(s) | 331, 1053 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 110, 140, 342, 372, 689, 719 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 688, 1060 |
| BseCI | ATCGAT | 1 cut(s) | 753 |
| BseDI | CCNNGG | 5 cut(s) | 400, 489, 538, 606, 909 |
| BseGI | GGATG | 5 cut(s) | 127, 214, 500, 512, 952 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 688, 1060 |
| BseRI | GAGGAG | 3 cut(s) | 344, 347, 926 |
| BseXI | GCAGC | 2 cut(s) | 565, 917 |
| BsgI | GTGCAG | 1 cut(s) | 564 |
| BshFI | GGCC | 1 cut(s) | 397 |
| BshVI | ATCGAT | 1 cut(s) | 753 |
| BsiHKCI | CYCGRG | 2 cut(s) | 401, 605 |
| BsiSI | CCGG | 3 cut(s) | 332, 685, 1054 |
| BslFI | GGGAC | 3 cut(s) | 418, 588, 833 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 688, 1060 |
| BsmAI | GTCTC | 3 cut(s) | 189, 321, 886 |
| BsmBI | CGTCTC | 1 cut(s) | 189 |
| BsmFI | GGGAC | 3 cut(s) | 418, 588, 833 |
| BsmI | GAATGC | 1 cut(s) | 566 |
| BsnI | GGCC | 1 cut(s) | 397 |
| Bso31I | GGTCTC | 1 cut(s) | 886 |
| BsoBI | CYCGRG | 2 cut(s) | 401, 605 |
| Bsp143I | GATC | 2 cut(s) | 754, 1066 |
| BspACI | CCGC | 3 cut(s) | 516, 626, 1064 |
| BspANI | GGCC | 1 cut(s) | 397 |
| BspDI | ATCGAT | 1 cut(s) | 753 |
| BspLI | GGNNCC | 1 cut(s) | 398 |
| BspPI | GGATC | 1 cut(s) | 1074 |
| BspTNI | GGTCTC | 1 cut(s) | 886 |
| BssECI | CCNNGG | 5 cut(s) | 400, 489, 538, 606, 909 |
| BssMI | GATC | 2 cut(s) | 754, 1066 |
| Bst4CI | ACNGT | 1 cut(s) | 1008 |
| Bst6I | CTCTTC | 4 cut(s) | 42, 105, 195, 771 |
| BstC8I | GCNNGC | 3 cut(s) | 424, 496, 637 |
| BstDSI | CCRYGG | 1 cut(s) | 489 |
| BstEII | GGTNACC | 1 cut(s) | 549 |
| BstF5I | GGATG | 5 cut(s) | 127, 214, 500, 512, 952 |
| BstHHI | GCGC | 1 cut(s) | 500 |
| BstKTI | GATC | 2 cut(s) | 757, 1069 |
| BstMAI | GTCTC | 3 cut(s) | 189, 321, 886 |
| BstMBI | GATC | 2 cut(s) | 754, 1066 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 575, 632 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 547 |
| BstPI | GGTNACC | 1 cut(s) | 549 |
| BstV1I | GCAGC | 2 cut(s) | 565, 917 |
| BstV2I | GAAGAC | 1 cut(s) | 777 |
| Bsu15I | ATCGAT | 1 cut(s) | 753 |
| BsuRI | GGCC | 1 cut(s) | 397 |
| BsuTUI | ATCGAT | 1 cut(s) | 753 |
| BtgI | CCRYGG | 1 cut(s) | 489 |
| BtrI | CACGTC | 1 cut(s) | 329 |
| BtsCI | GGATG | 5 cut(s) | 127, 214, 500, 512, 952 |
| Cac8I | GCNNGC | 3 cut(s) | 424, 496, 637 |
| CfoI | GCGC | 1 cut(s) | 500 |
| Cfr13I | GGNCC | 1 cut(s) | 396 |
| ClaI | ATCGAT | 1 cut(s) | 753 |
| Csp6I | GTAC | 3 cut(s) | 792, 1009, 1051 |
| CviQI | GTAC | 3 cut(s) | 792, 1009, 1051 |
| DpnI | GATC | 2 cut(s) | 756, 1068 |
| DpnII | GATC | 2 cut(s) | 754, 1066 |
| Eam1104I | CTCTTC | 4 cut(s) | 42, 105, 195, 771 |
| EarI | CTCTTC | 4 cut(s) | 42, 105, 195, 771 |
| EciI | GGCGGA | 1 cut(s) | 641 |
| Eco31I | GGTCTC | 1 cut(s) | 886 |
| Eco57I | CTGAAG | 1 cut(s) | 59 |
| Eco88I | CYCGRG | 2 cut(s) | 401, 605 |
| Eco91I | GGTNACC | 1 cut(s) | 549 |
| EcoO109I | RGGNCCY | 1 cut(s) | 396 |
| EcoO65I | GGTNACC | 1 cut(s) | 549 |
| EcoRI | GAATTC | 1 cut(s) | 455 |
| EcoT22I | ATGCAT | 1 cut(s) | 667 |
| Esp3I | CGTCTC | 1 cut(s) | 189 |
| FaiI | YATR | 6 cut(s) | 287, 312, 484, 732, 762, 808 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 909, 941 |
| FaqI | GGGAC | 3 cut(s) | 418, 588, 833 |
| Fnu4HI | GCNGC | 2 cut(s) | 579, 906 |
| FokI | GGATG | 5 cut(s) | 134, 221, 487, 499, 959 |
| Fsp4HI | GCNGC | 2 cut(s) | 579, 906 |
| FspBI | CTAG | 1 cut(s) | 371 |
| GlaI | GCGC | 1 cut(s) | 499 |
| GluI | GCNGC | 2 cut(s) | 579, 906 |
| GsuI | CTGGAG | 1 cut(s) | 53 |
| HaeIII | GGCC | 1 cut(s) | 397 |
| HapII | CCGG | 3 cut(s) | 332, 685, 1054 |
| HhaI | GCGC | 1 cut(s) | 500 |
| Hin6I | GCGC | 1 cut(s) | 498 |
| HinP1I | GCGC | 1 cut(s) | 498 |
| HinfI | GANTC | 2 cut(s) | 314, 527 |
| HpaII | CCGG | 3 cut(s) | 332, 685, 1054 |
| HphI | GGTGA | 5 cut(s) | 346, 543, 585, 757, 838 |
| Hpy188I | TCNGA | 1 cut(s) | 505 |
| Hpy188III | TCNNGA | 4 cut(s) | 77, 403, 460, 605 |
| HpyAV | CCTTC | 1 cut(s) | 199 |
| HpyCH4III | ACNGT | 1 cut(s) | 1008 |
| HpyCH4IV | ACGT | 1 cut(s) | 328 |
| HpyCH4V | TGCA | 4 cut(s) | 581, 639, 665, 734 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 575, 632 |
| HpySE526I | ACGT | 1 cut(s) | 328 |
| HspAI | GCGC | 1 cut(s) | 498 |
| Kzo9I | GATC | 2 cut(s) | 754, 1066 |
| LpnPI | CCDG | 9 cut(s) | 17, 62, 345, 437, 445, 613, 674, 698, 1067 |
| Lsp1109I | GCAGC | 2 cut(s) | 565, 917 |
| LweI | GCATC | 2 cut(s) | 509, 622 |
| MaeI | CTAG | 1 cut(s) | 371 |
| MaeII | ACGT | 1 cut(s) | 328 |
| MaeIII | GTNAC | 2 cut(s) | 549, 691 |
| MalI | GATC | 2 cut(s) | 756, 1068 |
| MboI | GATC | 2 cut(s) | 754, 1066 |
| MfeI | CAATTG | 1 cut(s) | 275 |
| MlyI | GAGTC | 2 cut(s) | 323, 521 |
| Mph1103I | ATGCAT | 1 cut(s) | 667 |
| MseI | TTAA | 5 cut(s) | 218, 261, 612, 660, 939 |
| MslI | CAYNNNNRTG | 2 cut(s) | 290, 559 |
| MspI | CCGG | 3 cut(s) | 332, 685, 1054 |
| MunI | CAATTG | 1 cut(s) | 275 |
| Mva1269I | GAATGC | 1 cut(s) | 566 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 575, 632 |
| NdeII | GATC | 2 cut(s) | 754, 1066 |
| NlaIV | GGNNCC | 1 cut(s) | 398 |
| NmeAIII | GCCGAG | 1 cut(s) | 934 |
| NmuCI | GTSAC | 1 cut(s) | 549 |
| NsiI | ATGCAT | 1 cut(s) | 667 |
| PctI | GAATGC | 1 cut(s) | 566 |
| PkrI | GCNGC | 2 cut(s) | 580, 907 |
| PleI | GAGTC | 2 cut(s) | 322, 521 |
| PpsI | GAGTC | 2 cut(s) | 322, 521 |
| PshAI | GACNNNNGTC | 1 cut(s) | 547 |
| PshBI | ATTAAT | 1 cut(s) | 939 |
| PspEI | GGTNACC | 1 cut(s) | 549 |
| PspN4I | GGNNCC | 1 cut(s) | 398 |
| PspPI | GGNCC | 1 cut(s) | 396 |
| RsaI | GTAC | 3 cut(s) | 793, 1010, 1052 |
| RsaNI | GTAC | 3 cut(s) | 792, 1009, 1051 |
| RseI | CAYNNNNRTG | 2 cut(s) | 290, 559 |
| SaqAI | TTAA | 5 cut(s) | 218, 261, 612, 660, 939 |
| SatI | GCNGC | 2 cut(s) | 579, 906 |
| Sau3AI | GATC | 2 cut(s) | 754, 1066 |
| Sau96I | GGNCC | 1 cut(s) | 396 |
| SchI | GAGTC | 2 cut(s) | 323, 521 |
| SfaNI | GCATC | 2 cut(s) | 509, 622 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 290, 559 |
| SmlI | CTYRAG | 1 cut(s) | 446 |
| SmoI | CTYRAG | 1 cut(s) | 446 |
| SsiI | CCGC | 3 cut(s) | 516, 626, 1064 |
| SspMI | CTAG | 1 cut(s) | 371 |
| TaaI | ACNGT | 1 cut(s) | 1008 |
| TaiI | ACGT | 1 cut(s) | 331 |
| TaqI | TCGA | 1 cut(s) | 753 |
| TaqII | GACCGA | 3 cut(s) | 537, 886, 910 |
| TatI | WGTACW | 1 cut(s) | 791 |
| Tru1I | TTAA | 5 cut(s) | 218, 261, 612, 660, 939 |
| Tru9I | TTAA | 5 cut(s) | 218, 261, 612, 660, 939 |
| TseFI | GTSAC | 1 cut(s) | 549 |
| TseI | GCWGC | 2 cut(s) | 578, 905 |
| Tsp45I | GTSAC | 1 cut(s) | 549 |
| TspDTI | ATGAA | 3 cut(s) | 17, 578, 987 |
| TspGWI | ACGGA | 1 cut(s) | 708 |
| VspI | ATTAAT | 1 cut(s) | 939 |
| XapI | RAATTY | 2 cut(s) | 455, 585 |
| XspI | CTAG | 1 cut(s) | 371 |
| Zsp2I | ATGCAT | 1 cut(s) | 667 |