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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 3 cut(s) | 188, 199, 511 |
| AcsI | RAATTY | 5 cut(s) | 173, 269, 337, 487, 572 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 127, 446 |
| AgsI | TTSAA | 3 cut(s) | 201, 563, 577 |
| AluBI | AGCT | 4 cut(s) | 317, 329, 534, 583 |
| AluI | AGCT | 4 cut(s) | 317, 329, 534, 583 |
| AlwI | GGATC | 3 cut(s) | 188, 199, 511 |
| ApoI | RAATTY | 5 cut(s) | 173, 269, 337, 487, 572 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 173, 457 |
| AspS9I | GGNCC | 1 cut(s) | 235 |
| AsuHPI | GGTGA | 1 cut(s) | 239 |
| AvaII | GGWCC | 1 cut(s) | 235 |
| BccI | CCATC | 1 cut(s) | 350 |
| BfaI | CTAG | 1 cut(s) | 218 |
| BfmI | CTRYAG | 2 cut(s) | 125, 318 |
| Bme18I | GGWCC | 1 cut(s) | 235 |
| BmgT120I | GGNCC | 1 cut(s) | 235 |
| BmiI | GGNNCC | 1 cut(s) | 237 |
| BmrI | ACTGGG | 1 cut(s) | 523 |
| BmsI | GCATC | 1 cut(s) | 46 |
| BmuI | ACTGGG | 1 cut(s) | 523 |
| BpuEI | CTTGAG | 1 cut(s) | 11 |
| BsaJI | CCNNGG | 1 cut(s) | 64 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 127, 446 |
| Bse1I | ACTGG | 1 cut(s) | 518 |
| BseDI | CCNNGG | 1 cut(s) | 64 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 127, 446 |
| BseMII | CTCAG | 1 cut(s) | 240 |
| BseNI | ACTGG | 1 cut(s) | 518 |
| BseRI | GAGGAG | 1 cut(s) | 95 |
| BslFI | GGGAC | 1 cut(s) | 221 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 127, 446 |
| BsmFI | GGGAC | 1 cut(s) | 221 |
| BsmI | GAATGC | 1 cut(s) | 59 |
| Bsp143I | GATC | 3 cut(s) | 180, 204, 503 |
| Bsp19I | CCATGG | 1 cut(s) | 64 |
| BspCNI | CTCAG | 1 cut(s) | 241 |
| BspLI | GGNNCC | 1 cut(s) | 237 |
| BspMAI | CTGCAG | 1 cut(s) | 129 |
| BspPI | GGATC | 3 cut(s) | 188, 199, 511 |
| BsrI | ACTGG | 1 cut(s) | 518 |
| BssECI | CCNNGG | 1 cut(s) | 64 |
| BssMI | GATC | 3 cut(s) | 180, 204, 503 |
| BssT1I | CCWWGG | 1 cut(s) | 64 |
| Bst6I | CTCTTC | 1 cut(s) | 158 |
| BstC8I | GCNNGC | 1 cut(s) | 129 |
| BstDEI | CTNAG | 1 cut(s) | 249 |
| BstDSI | CCRYGG | 1 cut(s) | 64 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 125 |
| BstKTI | GATC | 3 cut(s) | 183, 207, 506 |
| BstMBI | GATC | 3 cut(s) | 180, 204, 503 |
| BstSFI | CTRYAG | 2 cut(s) | 125, 318 |
| BstX2I | RGATCY | 3 cut(s) | 180, 204, 503 |
| BstYI | RGATCY | 3 cut(s) | 180, 204, 503 |
| BtgI | CCRYGG | 1 cut(s) | 64 |
| Cac8I | GCNNGC | 1 cut(s) | 129 |
| Cfr13I | GGNCC | 1 cut(s) | 235 |
| CviAII | CATG | 3 cut(s) | 65, 140, 462 |
| CviJI | RGCY | 8 cut(s) | 72, 92, 317, 329, 479, 534, 558, 583 |
| CviKI_1 | RGCY | 8 cut(s) | 72, 92, 317, 329, 479, 534, 558, 583 |
| DdeI | CTNAG | 1 cut(s) | 249 |
| DpnI | GATC | 3 cut(s) | 182, 206, 505 |
| DpnII | GATC | 3 cut(s) | 180, 204, 503 |
| Eam1104I | CTCTTC | 1 cut(s) | 158 |
| EarI | CTCTTC | 1 cut(s) | 158 |
| Eco130I | CCWWGG | 1 cut(s) | 64 |
| Eco47I | GGWCC | 1 cut(s) | 235 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 125 |
| EcoRI | GAATTC | 1 cut(s) | 572 |
| EcoT14I | CCWWGG | 1 cut(s) | 64 |
| EcoT22I | ATGCAT | 1 cut(s) | 61 |
| ErhI | CCWWGG | 1 cut(s) | 64 |
| FaeI | CATG | 3 cut(s) | 68, 143, 465 |
| FaqI | GGGAC | 1 cut(s) | 221 |
| FatI | CATG | 3 cut(s) | 64, 139, 461 |
| FauNDI | CATATG | 1 cut(s) | 587 |
| FspBI | CTAG | 1 cut(s) | 218 |
| Hin1II | CATG | 3 cut(s) | 68, 143, 465 |
| HindIII | AAGCTT | 1 cut(s) | 532 |
| HinfI | GANTC | 1 cut(s) | 4 |
| HphI | GGTGA | 1 cut(s) | 239 |
| Hpy188I | TCNGA | 3 cut(s) | 103, 250, 268 |
| Hpy188III | TCNNGA | 3 cut(s) | 178, 184, 277 |
| HpyAV | CCTTC | 2 cut(s) | 362, 548 |
| HpyCH4IV | ACGT | 1 cut(s) | 471 |
| HpyCH4V | TGCA | 5 cut(s) | 59, 127, 257, 334, 446 |
| HpyF3I | CTNAG | 1 cut(s) | 249 |
| HpySE526I | ACGT | 1 cut(s) | 471 |
| Hsp92II | CATG | 3 cut(s) | 68, 143, 465 |
| Kzo9I | GATC | 3 cut(s) | 180, 204, 503 |
| LpnPI | CCDG | 6 cut(s) | 21, 113, 163, 398, 432, 499 |
| LweI | GCATC | 1 cut(s) | 46 |
| MaeI | CTAG | 1 cut(s) | 218 |
| MaeII | ACGT | 1 cut(s) | 471 |
| MaeIII | GTNAC | 1 cut(s) | 464 |
| MalI | GATC | 3 cut(s) | 182, 206, 505 |
| MboI | GATC | 3 cut(s) | 180, 204, 503 |
| MboII | GAAGA | 5 cut(s) | 145, 148, 181, 391, 581 |
| MflI | RGATCY | 3 cut(s) | 180, 204, 503 |
| MluCI | AATT | 8 cut(s) | 173, 269, 303, 337, 390, 456, 487, 572 |
| MlyI | GAGTC | 1 cut(s) | 13 |
| MmeI | TCCRAC | 1 cut(s) | 508 |
| MnlI | CCTC | 5 cut(s) | 73, 128, 161, 244, 358 |
| Mph1103I | ATGCAT | 1 cut(s) | 61 |
| MroXI | GAANNNNTTC | 2 cut(s) | 173, 457 |
| MseI | TTAA | 2 cut(s) | 306, 404 |
| MslI | CAYNNNNRTG | 1 cut(s) | 144 |
| Mva1269I | GAATGC | 1 cut(s) | 59 |
| NcoI | CCATGG | 1 cut(s) | 64 |
| NdeI | CATATG | 1 cut(s) | 587 |
| NdeII | GATC | 3 cut(s) | 180, 204, 503 |
| NlaIII | CATG | 3 cut(s) | 68, 143, 465 |
| NlaIV | GGNNCC | 1 cut(s) | 237 |
| NmuCI | GTSAC | 1 cut(s) | 464 |
| NsiI | ATGCAT | 1 cut(s) | 61 |
| PctI | GAATGC | 1 cut(s) | 59 |
| PdmI | GAANNNNTTC | 2 cut(s) | 173, 457 |
| PflFI | GACNNNGTC | 1 cut(s) | 470 |
| PleI | GAGTC | 1 cut(s) | 12 |
| PpsI | GAGTC | 1 cut(s) | 12 |
| PspN4I | GGNNCC | 1 cut(s) | 237 |
| PspPI | GGNCC | 1 cut(s) | 235 |
| PstI | CTGCAG | 1 cut(s) | 129 |
| PsuI | RGATCY | 3 cut(s) | 180, 204, 503 |
| PsyI | GACNNNGTC | 1 cut(s) | 470 |
| RseI | CAYNNNNRTG | 1 cut(s) | 144 |
| SaqAI | TTAA | 2 cut(s) | 306, 404 |
| Sau3AI | GATC | 3 cut(s) | 180, 204, 503 |
| Sau96I | GGNCC | 1 cut(s) | 235 |
| SchI | GAGTC | 1 cut(s) | 13 |
| SetI | ASST | 8 cut(s) | 153, 229, 319, 331, 404, 474, 536, 585 |
| SfaNI | GCATC | 1 cut(s) | 46 |
| SfcI | CTRYAG | 2 cut(s) | 125, 318 |
| SinI | GGWCC | 1 cut(s) | 235 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 144 |
| SmlI | CTYRAG | 1 cut(s) | 26 |
| SmoI | CTYRAG | 1 cut(s) | 26 |
| Sse9I | AATT | 8 cut(s) | 173, 269, 303, 337, 390, 456, 487, 572 |
| SspI | AATATT | 1 cut(s) | 49 |
| SspMI | CTAG | 1 cut(s) | 218 |
| StyI | CCWWGG | 1 cut(s) | 64 |
| TaiI | ACGT | 1 cut(s) | 474 |
| TaqI | TCGA | 2 cut(s) | 185, 276 |
| TasI | AATT | 8 cut(s) | 173, 269, 303, 337, 390, 456, 487, 572 |
| Tru1I | TTAA | 2 cut(s) | 306, 404 |
| Tru9I | TTAA | 2 cut(s) | 306, 404 |
| TseFI | GTSAC | 1 cut(s) | 464 |
| Tsp45I | GTSAC | 1 cut(s) | 464 |
| TspDTI | ATGAA | 5 cut(s) | 100, 156, 450, 526, 567 |
| Tth111I | GACNNNGTC | 1 cut(s) | 470 |
| VpaK11BI | GGWCC | 1 cut(s) | 235 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 125 |
| XapI | RAATTY | 5 cut(s) | 173, 269, 337, 487, 572 |
| XmnI | GAANNNNTTC | 2 cut(s) | 173, 457 |
| XspI | CTAG | 1 cut(s) | 218 |
| Zsp2I | ATGCAT | 1 cut(s) | 61 |