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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 38 |
| AfaI | GTAC | 1 cut(s) | 232 |
| AgsI | TTSAA | 2 cut(s) | 212, 224 |
| Alw26I | GTCTC | 2 cut(s) | 71, 344 |
| Ama87I | CYCGRG | 2 cut(s) | 74, 131 |
| ApoI | RAATTY | 1 cut(s) | 38 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 86, 118 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 219 |
| AvaI | CYCGRG | 2 cut(s) | 74, 131 |
| BanII | GRGCYC | 1 cut(s) | 138 |
| BbsI | GAAGAC | 1 cut(s) | 263 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 86, 120 |
| BcoDI | GTCTC | 2 cut(s) | 71, 344 |
| BfmI | CTRYAG | 1 cut(s) | 204 |
| BmeT110I | CYCGRG | 2 cut(s) | 74, 131 |
| BmiI | GGNNCC | 2 cut(s) | 64, 272 |
| BoxI | GACNNNNGTC | 1 cut(s) | 184 |
| BpiI | GAAGAC | 1 cut(s) | 263 |
| BsaI | GGTCTC | 1 cut(s) | 344 |
| Bsh1285I | CGRYCG | 1 cut(s) | 100 |
| BsiEI | CGRYCG | 1 cut(s) | 100 |
| BsiHKCI | CYCGRG | 2 cut(s) | 74, 131 |
| BsiSI | CCGG | 1 cut(s) | 60 |
| BsiWI | CGTACG | 1 cut(s) | 230 |
| BsmAI | GTCTC | 2 cut(s) | 71, 344 |
| Bso31I | GGTCTC | 1 cut(s) | 344 |
| BsoBI | CYCGRG | 2 cut(s) | 74, 131 |
| Bsp1286I | GDGCHC | 1 cut(s) | 138 |
| BspLI | GGNNCC | 2 cut(s) | 64, 272 |
| BspTNI | GGTCTC | 1 cut(s) | 344 |
| Bst4CI | ACNGT | 2 cut(s) | 101, 325 |
| Bst6I | CTCTTC | 1 cut(s) | 56 |
| BstMAI | GTCTC | 2 cut(s) | 71, 344 |
| BstMCI | CGRYCG | 1 cut(s) | 100 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 156 |
| BstNSI | RCATGY | 1 cut(s) | 150 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 184 |
| BstSFI | CTRYAG | 1 cut(s) | 204 |
| BstV2I | GAAGAC | 1 cut(s) | 263 |
| Csp6I | GTAC | 1 cut(s) | 231 |
| CviAII | CATG | 1 cut(s) | 147 |
| CviJI | RGCY | 5 cut(s) | 63, 130, 136, 177, 273 |
| CviKI_1 | RGCY | 5 cut(s) | 63, 130, 136, 177, 273 |
| CviQI | GTAC | 1 cut(s) | 231 |
| Eam1104I | CTCTTC | 1 cut(s) | 56 |
| EarI | CTCTTC | 1 cut(s) | 56 |
| Eco24I | GRGCYC | 1 cut(s) | 138 |
| Eco31I | GGTCTC | 1 cut(s) | 344 |
| Eco88I | CYCGRG | 2 cut(s) | 74, 131 |
| EcoRI | GAATTC | 1 cut(s) | 38 |
| EcoT38I | GRGCYC | 1 cut(s) | 138 |
| FaeI | CATG | 1 cut(s) | 150 |
| FaiI | YATR | 6 cut(s) | 84, 117, 148, 206, 242, 318 |
| FatI | CATG | 1 cut(s) | 146 |
| FriOI | GRGCYC | 1 cut(s) | 138 |
| HapII | CCGG | 1 cut(s) | 60 |
| Hin1II | CATG | 1 cut(s) | 150 |
| HincII | GTYRAC | 1 cut(s) | 288 |
| HindII | GTYRAC | 1 cut(s) | 288 |
| HinfI | GANTC | 1 cut(s) | 122 |
| HpaI | GTTAAC | 1 cut(s) | 288 |
| HpaII | CCGG | 1 cut(s) | 60 |
| Hpy166II | GTNNAC | 1 cut(s) | 288 |
| Hpy188I | TCNGA | 2 cut(s) | 29, 94 |
| Hpy188III | TCNNGA | 2 cut(s) | 74, 76 |
| Hpy8I | GTNNAC | 1 cut(s) | 288 |
| Hpy99I | CGWCG | 1 cut(s) | 98 |
| HpyCH4III | ACNGT | 2 cut(s) | 101, 325 |
| HpyCH4IV | ACGT | 2 cut(s) | 233, 351 |
| HpyCH4V | TGCA | 1 cut(s) | 295 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 156 |
| HpySE526I | ACGT | 2 cut(s) | 233, 351 |
| Hsp92II | CATG | 1 cut(s) | 150 |
| KspAI | GTTAAC | 1 cut(s) | 288 |
| LmnI | GCTCC | 2 cut(s) | 68, 270 |
| LpnPI | CCDG | 2 cut(s) | 73, 276 |
| MaeII | ACGT | 2 cut(s) | 233, 351 |
| MaeIII | GTNAC | 1 cut(s) | 123 |
| MboII | GAAGA | 5 cut(s) | 43, 224, 265, 268, 387 |
| MhlI | GDGCHC | 1 cut(s) | 138 |
| MluCI | AATT | 1 cut(s) | 38 |
| MlyI | GAGTC | 1 cut(s) | 131 |
| MmeI | TCCRAC | 1 cut(s) | 117 |
| MnlI | CCTC | 1 cut(s) | 59 |
| MroXI | GAANNNNTTC | 1 cut(s) | 219 |
| MseI | TTAA | 3 cut(s) | 44, 287, 362 |
| MslI | CAYNNNNRTG | 1 cut(s) | 315 |
| MspI | CCGG | 1 cut(s) | 60 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 156 |
| NlaIII | CATG | 1 cut(s) | 150 |
| NlaIV | GGNNCC | 2 cut(s) | 64, 272 |
| NmuCI | GTSAC | 1 cut(s) | 123 |
| NspI | RCATGY | 1 cut(s) | 150 |
| PaeR7I | CTCGAG | 1 cut(s) | 74 |
| PdmI | GAANNNNTTC | 1 cut(s) | 219 |
| Pfl23II | CGTACG | 1 cut(s) | 230 |
| PleI | GAGTC | 1 cut(s) | 130 |
| PpsI | GAGTC | 1 cut(s) | 130 |
| PshAI | GACNNNNGTC | 1 cut(s) | 184 |
| PspLI | CGTACG | 1 cut(s) | 230 |
| PspN4I | GGNNCC | 2 cut(s) | 64, 272 |
| RsaI | GTAC | 1 cut(s) | 232 |
| RsaNI | GTAC | 1 cut(s) | 231 |
| RseI | CAYNNNNRTG | 1 cut(s) | 315 |
| SaqAI | TTAA | 3 cut(s) | 44, 287, 362 |
| SchI | GAGTC | 1 cut(s) | 131 |
| SduI | GDGCHC | 1 cut(s) | 138 |
| SetI | ASST | 3 cut(s) | 236, 300, 354 |
| SfcI | CTRYAG | 1 cut(s) | 204 |
| Sfr274I | CTCGAG | 1 cut(s) | 74 |
| SlaI | CTCGAG | 1 cut(s) | 74 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 315 |
| SmlI | CTYRAG | 1 cut(s) | 74 |
| SmoI | CTYRAG | 1 cut(s) | 74 |
| Sse9I | AATT | 1 cut(s) | 38 |
| TaaI | ACNGT | 2 cut(s) | 101, 325 |
| TaiI | ACGT | 2 cut(s) | 236, 354 |
| TaqI | TCGA | 2 cut(s) | 75, 188 |
| TasI | AATT | 1 cut(s) | 38 |
| Tru1I | TTAA | 3 cut(s) | 44, 287, 362 |
| Tru9I | TTAA | 3 cut(s) | 44, 287, 362 |
| TseFI | GTSAC | 1 cut(s) | 123 |
| Tsp45I | GTSAC | 1 cut(s) | 123 |
| TspDTI | ATGAA | 2 cut(s) | 43, 333 |
| XapI | RAATTY | 1 cut(s) | 38 |
| XceI | RCATGY | 1 cut(s) | 150 |
| XhoI | CTCGAG | 1 cut(s) | 74 |
| XmnI | GAANNNNTTC | 1 cut(s) | 219 |