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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 900 |
| Acc36I | ACCTGC | 1 cut(s) | 589 |
| AccII | CGCG | 1 cut(s) | 794 |
| AciI | CCGC | 2 cut(s) | 470, 485 |
| AcyI | GRCGYC | 1 cut(s) | 897 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 788 |
| AgsI | TTSAA | 3 cut(s) | 134, 256, 662 |
| AjiI | CACGTC | 1 cut(s) | 431 |
| AjnI | CCWGG | 1 cut(s) | 433 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 270, 302 |
| AluBI | AGCT | 3 cut(s) | 223, 244, 680 |
| AluI | AGCT | 3 cut(s) | 223, 244, 680 |
| Alw26I | GTCTC | 1 cut(s) | 167 |
| AoxI | GGCC | 1 cut(s) | 498 |
| ApeKI | GCWGC | 3 cut(s) | 620, 677, 680 |
| AspLEI | GCGC | 2 cut(s) | 602, 794 |
| AspS9I | GGNCC | 1 cut(s) | 499 |
| AsuC2I | CCSGG | 1 cut(s) | 789 |
| AsuHPI | GGTGA | 3 cut(s) | 34, 687, 864 |
| AsuII | TTCGAA | 1 cut(s) | 828 |
| AxyI | CCTNAGG | 2 cut(s) | 504, 708 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 839, 872 |
| BbsI | GAAGAC | 1 cut(s) | 194 |
| BbvI | GCAGC | 3 cut(s) | 632, 667, 689 |
| BccI | CCATC | 4 cut(s) | 107, 163, 388, 604 |
| BciT130I | CCWGG | 1 cut(s) | 435 |
| BcnI | CCSGG | 1 cut(s) | 789 |
| BcoDI | GTCTC | 1 cut(s) | 167 |
| BfaI | CTAG | 1 cut(s) | 474 |
| BfmI | CTRYAG | 2 cut(s) | 618, 924 |
| BfuAI | ACCTGC | 1 cut(s) | 589 |
| BisI | GCNGC | 3 cut(s) | 621, 678, 681 |
| BlsI | GCNGC | 3 cut(s) | 622, 679, 682 |
| Bme1390I | CCNGG | 2 cut(s) | 435, 789 |
| BmgBI | CACGTC | 1 cut(s) | 431 |
| BmgT120I | GGNCC | 1 cut(s) | 499 |
| BmiI | GGNNCC | 1 cut(s) | 501 |
| BmrFI | CCNGG | 2 cut(s) | 435, 789 |
| BmsI | GCATC | 4 cut(s) | 43, 611, 623, 724 |
| BoxI | GACNNNNGTC | 1 cut(s) | 649 |
| BpiI | GAAGAC | 1 cut(s) | 194 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 338, 370 |
| BpmI | CTGGAG | 1 cut(s) | 245 |
| Bpu14I | TTCGAA | 1 cut(s) | 828 |
| BpuEI | CTTGAG | 1 cut(s) | 534 |
| BpuMI | CCSGG | 1 cut(s) | 789 |
| BsaBI | GATNNNNATC | 1 cut(s) | 29 |
| BsaHI | GRCGYC | 1 cut(s) | 897 |
| BsaJI | CCNNGG | 1 cut(s) | 640 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 445, 475, 793, 823 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 788 |
| Bse1I | ACTGG | 2 cut(s) | 144, 905 |
| Bse21I | CCTNAGG | 2 cut(s) | 504, 708 |
| Bse8I | GATNNNNATC | 1 cut(s) | 29 |
| BseBI | CCWGG | 1 cut(s) | 435 |
| BseDI | CCNNGG | 1 cut(s) | 640 |
| BseGI | GGATG | 2 cut(s) | 166, 319 |
| BseJI | GATNNNNATC | 1 cut(s) | 29 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 788 |
| BseMII | CTCAG | 5 cut(s) | 63, 396, 518, 597, 699 |
| BseNI | ACTGG | 2 cut(s) | 144, 905 |
| BseRI | GAGGAG | 3 cut(s) | 284, 447, 450 |
| BseXI | GCAGC | 3 cut(s) | 632, 667, 689 |
| BsgI | GTGCAG | 1 cut(s) | 666 |
| Bsh1236I | CGCG | 1 cut(s) | 794 |
| BshFI | GGCC | 1 cut(s) | 500 |
| BsiSI | CCGG | 1 cut(s) | 788 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 788 |
| BsmAI | GTCTC | 1 cut(s) | 167 |
| BsnI | GGCC | 1 cut(s) | 500 |
| Bsp119I | TTCGAA | 1 cut(s) | 828 |
| Bsp143I | GATC | 1 cut(s) | 861 |
| BspACI | CCGC | 2 cut(s) | 470, 485 |
| BspANI | GGCC | 1 cut(s) | 500 |
| BspCNI | CTCAG | 5 cut(s) | 64, 397, 517, 598, 700 |
| BspFNI | CGCG | 1 cut(s) | 794 |
| BspLI | GGNNCC | 1 cut(s) | 501 |
| BspMAI | CTGCAG | 1 cut(s) | 622 |
| BspMI | ACCTGC | 1 cut(s) | 589 |
| BspQI | GCTCTTC | 1 cut(s) | 234 |
| BspT104I | TTCGAA | 1 cut(s) | 828 |
| BsrI | ACTGG | 2 cut(s) | 144, 905 |
| BssECI | CCNNGG | 1 cut(s) | 640 |
| BssMI | GATC | 1 cut(s) | 861 |
| BssNI | GRCGYC | 1 cut(s) | 897 |
| BssT1I | CCWWGG | 1 cut(s) | 640 |
| Bst2UI | CCWGG | 1 cut(s) | 435 |
| Bst6I | CTCTTC | 3 cut(s) | 192, 234, 297 |
| BstACI | GRCGYC | 1 cut(s) | 897 |
| BstBI | TTCGAA | 1 cut(s) | 828 |
| BstC8I | GCNNGC | 4 cut(s) | 527, 586, 598, 739 |
| BstDEI | CTNAG | 6 cut(s) | 72, 245, 405, 504, 606, 708 |
| BstF5I | GGATG | 2 cut(s) | 166, 319 |
| BstFNI | CGCG | 1 cut(s) | 794 |
| BstHHI | GCGC | 2 cut(s) | 602, 794 |
| BstKTI | GATC | 1 cut(s) | 864 |
| BstMAI | GTCTC | 1 cut(s) | 167 |
| BstMBI | GATC | 1 cut(s) | 861 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 620, 677, 844 |
| BstNI | CCWGG | 1 cut(s) | 435 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 649 |
| BstSCI | CCNGG | 2 cut(s) | 433, 787 |
| BstSFI | CTRYAG | 2 cut(s) | 618, 924 |
| BstUI | CGCG | 1 cut(s) | 794 |
| BstV1I | GCAGC | 3 cut(s) | 632, 667, 689 |
| BstV2I | GAAGAC | 1 cut(s) | 194 |
| Bsu36I | CCTNAGG | 2 cut(s) | 504, 708 |
| BsuRI | GGCC | 1 cut(s) | 500 |
| BtgZI | GCGATG | 1 cut(s) | 453 |
| BtrI | CACGTC | 1 cut(s) | 431 |
| BtsCI | GGATG | 2 cut(s) | 166, 319 |
| BtsIMutI | CAGTG | 1 cut(s) | 151 |
| BveI | ACCTGC | 1 cut(s) | 589 |
| Cac8I | GCNNGC | 4 cut(s) | 527, 586, 598, 739 |
| CfoI | GCGC | 2 cut(s) | 602, 794 |
| Cfr13I | GGNCC | 1 cut(s) | 499 |
| CviJI | RGCY | 8 cut(s) | 223, 244, 353, 500, 514, 680, 847, 886 |
| CviKI_1 | RGCY | 8 cut(s) | 223, 244, 353, 500, 514, 680, 847, 886 |
| DdeI | CTNAG | 6 cut(s) | 72, 245, 405, 504, 606, 708 |
| DpnI | GATC | 1 cut(s) | 863 |
| DpnII | GATC | 1 cut(s) | 861 |
| Eam1104I | CTCTTC | 3 cut(s) | 192, 234, 297 |
| EarI | CTCTTC | 3 cut(s) | 192, 234, 297 |
| EciI | GGCGGA | 1 cut(s) | 474 |
| Eco130I | CCWWGG | 1 cut(s) | 640 |
| Eco81I | CCTNAGG | 2 cut(s) | 504, 708 |
| EcoO109I | RGGNCCY | 1 cut(s) | 499 |
| EcoRII | CCWGG | 1 cut(s) | 433 |
| EcoT14I | CCWWGG | 1 cut(s) | 640 |
| EcoT22I | ATGCAT | 1 cut(s) | 769 |
| ErhI | CCWWGG | 1 cut(s) | 640 |
| FaiI | YATR | 3 cut(s) | 389, 836, 869 |
| Fnu4HI | GCNGC | 3 cut(s) | 621, 678, 681 |
| FokI | GGATG | 2 cut(s) | 173, 326 |
| Fsp4HI | GCNGC | 3 cut(s) | 621, 678, 681 |
| FspBI | CTAG | 1 cut(s) | 474 |
| GlaI | GCGC | 2 cut(s) | 601, 793 |
| GluI | GCNGC | 3 cut(s) | 621, 678, 681 |
| GsuI | CTGGAG | 1 cut(s) | 245 |
| HaeIII | GGCC | 1 cut(s) | 500 |
| HapII | CCGG | 1 cut(s) | 788 |
| HhaI | GCGC | 2 cut(s) | 602, 794 |
| Hin1I | GRCGYC | 1 cut(s) | 897 |
| Hin6I | GCGC | 2 cut(s) | 600, 792 |
| HinP1I | GCGC | 2 cut(s) | 600, 792 |
| HincII | GTYRAC | 1 cut(s) | 654 |
| HindII | GTYRAC | 1 cut(s) | 654 |
| HinfI | GANTC | 5 cut(s) | 68, 74, 416, 449, 688 |
| HpaII | CCGG | 1 cut(s) | 788 |
| HphI | GGTGA | 3 cut(s) | 34, 687, 864 |
| Hpy166II | GTNNAC | 1 cut(s) | 654 |
| Hpy188I | TCNGA | 3 cut(s) | 73, 607, 861 |
| Hpy188III | TCNNGA | 2 cut(s) | 506, 707 |
| Hpy8I | GTNNAC | 1 cut(s) | 654 |
| Hpy99I | CGWCG | 1 cut(s) | 902 |
| HpyCH4IV | ACGT | 2 cut(s) | 430, 897 |
| HpyCH4V | TGCA | 6 cut(s) | 56, 620, 683, 741, 767, 838 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 620, 677, 844 |
| HpyF3I | CTNAG | 6 cut(s) | 72, 245, 405, 504, 606, 708 |
| HpySE526I | ACGT | 2 cut(s) | 430, 897 |
| Hsp92I | GRCGYC | 1 cut(s) | 897 |
| HspAI | GCGC | 2 cut(s) | 600, 792 |
| Kzo9I | GATC | 1 cut(s) | 861 |
| LguI | GCTCTTC | 1 cut(s) | 234 |
| LmnI | GCTCC | 1 cut(s) | 593 |
| Lsp1109I | GCAGC | 3 cut(s) | 632, 667, 689 |
| LweI | GCATC | 4 cut(s) | 43, 611, 623, 724 |
| MaeI | CTAG | 1 cut(s) | 474 |
| MaeII | ACGT | 2 cut(s) | 430, 897 |
| MaeIII | GTNAC | 1 cut(s) | 795 |
| MalI | GATC | 1 cut(s) | 863 |
| MboI | GATC | 1 cut(s) | 861 |
| MluCI | AATT | 6 cut(s) | 209, 285, 342, 360, 716, 910 |
| MlyI | GAGTC | 3 cut(s) | 83, 425, 443 |
| Mph1103I | ATGCAT | 1 cut(s) | 769 |
| MseI | TTAA | 5 cut(s) | 45, 156, 363, 714, 762 |
| MslI | CAYNNNNRTG | 2 cut(s) | 392, 661 |
| MspA1I | CMGCKG | 1 cut(s) | 680 |
| MspI | CCGG | 1 cut(s) | 788 |
| MspR9I | CCNGG | 2 cut(s) | 435, 789 |
| MvaI | CCWGG | 1 cut(s) | 435 |
| MvnI | CGCG | 1 cut(s) | 794 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 620, 677, 844 |
| NciI | CCSGG | 1 cut(s) | 789 |
| NdeII | GATC | 1 cut(s) | 861 |
| NlaIV | GGNNCC | 1 cut(s) | 501 |
| NsiI | ATGCAT | 1 cut(s) | 769 |
| NspV | TTCGAA | 1 cut(s) | 828 |
| PciSI | GCTCTTC | 1 cut(s) | 234 |
| PfeI | GAWTC | 2 cut(s) | 68, 688 |
| PkrI | GCNGC | 3 cut(s) | 622, 679, 682 |
| PleI | GAGTC | 3 cut(s) | 82, 424, 443 |
| PpsI | GAGTC | 3 cut(s) | 82, 424, 443 |
| PshAI | GACNNNNGTC | 1 cut(s) | 649 |
| Psp6I | CCWGG | 1 cut(s) | 433 |
| PspGI | CCWGG | 1 cut(s) | 433 |
| PspN4I | GGNNCC | 1 cut(s) | 501 |
| PspPI | GGNCC | 1 cut(s) | 499 |
| PstI | CTGCAG | 1 cut(s) | 622 |
| PvuII | CAGCTG | 1 cut(s) | 680 |
| RseI | CAYNNNNRTG | 2 cut(s) | 392, 661 |
| SapI | GCTCTTC | 1 cut(s) | 234 |
| SaqAI | TTAA | 5 cut(s) | 45, 156, 363, 714, 762 |
| SatI | GCNGC | 3 cut(s) | 621, 678, 681 |
| Sau3AI | GATC | 1 cut(s) | 861 |
| Sau96I | GGNCC | 1 cut(s) | 499 |
| SchI | GAGTC | 3 cut(s) | 83, 425, 443 |
| ScrFI | CCNGG | 2 cut(s) | 435, 789 |
| SfaNI | GCATC | 4 cut(s) | 43, 611, 623, 724 |
| SfcI | CTRYAG | 2 cut(s) | 618, 924 |
| SfuI | TTCGAA | 1 cut(s) | 828 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 392, 661 |
| SmlI | CTYRAG | 1 cut(s) | 549 |
| SmoI | CTYRAG | 1 cut(s) | 549 |
| Sse9I | AATT | 6 cut(s) | 209, 285, 342, 360, 716, 910 |
| SsiI | CCGC | 2 cut(s) | 470, 485 |
| SspMI | CTAG | 1 cut(s) | 474 |
| StyD4I | CCNGG | 2 cut(s) | 433, 787 |
| StyI | CCWWGG | 1 cut(s) | 640 |
| TaiI | ACGT | 2 cut(s) | 433, 900 |
| TaqI | TCGA | 2 cut(s) | 828, 900 |
| TasI | AATT | 6 cut(s) | 209, 285, 342, 360, 716, 910 |
| TfiI | GAWTC | 2 cut(s) | 68, 688 |
| Tru1I | TTAA | 5 cut(s) | 45, 156, 363, 714, 762 |
| Tru9I | TTAA | 5 cut(s) | 45, 156, 363, 714, 762 |
| TscAI | CASTG | 1 cut(s) | 151 |
| TseI | GCWGC | 3 cut(s) | 620, 677, 680 |
| TspDTI | ATGAA | 3 cut(s) | 41, 210, 680 |
| TspGWI | ACGGA | 1 cut(s) | 743 |
| TspRI | CASTG | 1 cut(s) | 151 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 753 |
| XspI | CTAG | 1 cut(s) | 474 |
| ZraI | GACGTC | 1 cut(s) | 898 |
| Zsp2I | ATGCAT | 1 cut(s) | 769 |