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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 185 |
| AciI | CCGC | 2 cut(s) | 239, 463 |
| AclWI | GGATC | 2 cut(s) | 589, 602 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 179 |
| AgsI | TTSAA | 7 cut(s) | 53, 275, 293, 410, 517, 527, 604 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 209 |
| AhlI | ACTAGT | 1 cut(s) | 295 |
| AjnI | CCWGG | 1 cut(s) | 178 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 547, 579 |
| AluBI | AGCT | 3 cut(s) | 71, 335, 421 |
| AluI | AGCT | 3 cut(s) | 71, 335, 421 |
| AlwI | GGATC | 2 cut(s) | 589, 602 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 618 |
| AoxI | GGCC | 1 cut(s) | 391 |
| ApeKI | GCWGC | 3 cut(s) | 68, 71, 460 |
| AseI | ATTAAT | 1 cut(s) | 153 |
| AspLEI | GCGC | 1 cut(s) | 185 |
| AsuHPI | GGTGA | 3 cut(s) | 36, 78, 334 |
| AxyI | CCTNAGG | 1 cut(s) | 99 |
| BamHI | GGATCC | 1 cut(s) | 594 |
| BbvI | GCAGC | 3 cut(s) | 58, 80, 447 |
| BccI | CCATC | 1 cut(s) | 514 |
| BciT130I | CCWGG | 1 cut(s) | 180 |
| BcuI | ACTAGT | 1 cut(s) | 295 |
| BfaI | CTAG | 2 cut(s) | 266, 296 |
| BfmI | CTRYAG | 1 cut(s) | 472 |
| BisI | GCNGC | 5 cut(s) | 69, 72, 239, 461, 464 |
| BlsI | GCNGC | 5 cut(s) | 70, 73, 240, 462, 465 |
| Bme1390I | CCNGG | 1 cut(s) | 180 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 209 |
| BmiI | GGNNCC | 1 cut(s) | 596 |
| BmrFI | CCNGG | 1 cut(s) | 180 |
| BmsI | GCATC | 1 cut(s) | 115 |
| BoxI | GACNNNNGTC | 1 cut(s) | 40 |
| BpmI | CTGGAG | 1 cut(s) | 162 |
| BsaJI | CCNNGG | 1 cut(s) | 31 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 179 |
| Bse21I | CCTNAGG | 1 cut(s) | 99 |
| BseBI | CCWGG | 1 cut(s) | 180 |
| BseDI | CCNNGG | 1 cut(s) | 31 |
| BseGI | GGATG | 1 cut(s) | 432 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 179 |
| BseMII | CTCAG | 3 cut(s) | 90, 327, 393 |
| BseXI | GCAGC | 3 cut(s) | 58, 80, 447 |
| BsgI | GTGCAG | 1 cut(s) | 57 |
| Bsh1236I | CGCG | 1 cut(s) | 185 |
| BshFI | GGCC | 1 cut(s) | 393 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 179 |
| BsmI | GAATGC | 1 cut(s) | 483 |
| BsnI | GGCC | 1 cut(s) | 393 |
| Bsp143I | GATC | 4 cut(s) | 191, 252, 442, 594 |
| BspACI | CCGC | 2 cut(s) | 239, 463 |
| BspANI | GGCC | 1 cut(s) | 393 |
| BspCNI | CTCAG | 3 cut(s) | 91, 328, 392 |
| BspFNI | CGCG | 1 cut(s) | 185 |
| BspLI | GGNNCC | 1 cut(s) | 596 |
| BspMAI | CTGCAG | 1 cut(s) | 476 |
| BspPI | GGATC | 2 cut(s) | 589, 602 |
| BssECI | CCNNGG | 1 cut(s) | 31 |
| BssMI | GATC | 4 cut(s) | 191, 252, 442, 594 |
| Bst2UI | CCWGG | 1 cut(s) | 180 |
| BstC8I | GCNNGC | 2 cut(s) | 130, 231 |
| BstDEI | CTNAG | 3 cut(s) | 99, 336, 379 |
| BstF5I | GGATG | 1 cut(s) | 432 |
| BstFNI | CGCG | 1 cut(s) | 185 |
| BstHHI | GCGC | 1 cut(s) | 185 |
| BstKTI | GATC | 4 cut(s) | 194, 255, 445, 597 |
| BstMBI | GATC | 4 cut(s) | 191, 252, 442, 594 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 68, 235 |
| BstNI | CCWGG | 1 cut(s) | 180 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 40 |
| BstSCI | CCNGG | 1 cut(s) | 178 |
| BstSFI | CTRYAG | 1 cut(s) | 472 |
| BstUI | CGCG | 1 cut(s) | 185 |
| BstV1I | GCAGC | 3 cut(s) | 58, 80, 447 |
| BstX2I | RGATCY | 1 cut(s) | 594 |
| BstYI | RGATCY | 1 cut(s) | 594 |
| Bsu36I | CCTNAGG | 1 cut(s) | 99 |
| BsuRI | GGCC | 1 cut(s) | 393 |
| BtgZI | GCGATG | 1 cut(s) | 555 |
| BtsCI | GGATG | 1 cut(s) | 432 |
| Cac8I | GCNNGC | 2 cut(s) | 130, 231 |
| CaiI | CAGNNNCTG | 1 cut(s) | 618 |
| CfoI | GCGC | 1 cut(s) | 185 |
| CviAII | CATG | 1 cut(s) | 346 |
| CviJI | RGCY | 5 cut(s) | 71, 238, 335, 393, 421 |
| CviKI_1 | RGCY | 5 cut(s) | 71, 238, 335, 393, 421 |
| DdeI | CTNAG | 3 cut(s) | 99, 336, 379 |
| DpnI | GATC | 4 cut(s) | 193, 254, 444, 596 |
| DpnII | GATC | 4 cut(s) | 191, 252, 442, 594 |
| DriI | GACNNNNNGTC | 1 cut(s) | 209 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 209 |
| Eco81I | CCTNAGG | 1 cut(s) | 99 |
| EcoRII | CCWGG | 1 cut(s) | 178 |
| EcoT22I | ATGCAT | 1 cut(s) | 160 |
| FaeI | CATG | 1 cut(s) | 349 |
| FaiI | YATR | 7 cut(s) | 227, 260, 347, 365, 405, 458, 582 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 583, 615 |
| FatI | CATG | 1 cut(s) | 345 |
| Fnu4HI | GCNGC | 5 cut(s) | 69, 72, 239, 461, 464 |
| FokI | GGATG | 1 cut(s) | 439 |
| Fsp4HI | GCNGC | 5 cut(s) | 69, 72, 239, 461, 464 |
| FspBI | CTAG | 2 cut(s) | 266, 296 |
| GlaI | GCGC | 1 cut(s) | 184 |
| GluI | GCNGC | 5 cut(s) | 69, 72, 239, 461, 464 |
| GsuI | CTGGAG | 1 cut(s) | 162 |
| HaeIII | GGCC | 1 cut(s) | 393 |
| HhaI | GCGC | 1 cut(s) | 185 |
| Hin1II | CATG | 1 cut(s) | 349 |
| Hin6I | GCGC | 1 cut(s) | 183 |
| HinP1I | GCGC | 1 cut(s) | 183 |
| HinfI | GANTC | 4 cut(s) | 79, 383, 493, 615 |
| HphI | GGTGA | 3 cut(s) | 36, 78, 334 |
| Hpy166II | GTNNAC | 1 cut(s) | 342 |
| Hpy188I | TCNGA | 3 cut(s) | 252, 375, 382 |
| Hpy188III | TCNNGA | 1 cut(s) | 98 |
| Hpy8I | GTNNAC | 1 cut(s) | 342 |
| Hpy99I | CGWCG | 1 cut(s) | 207 |
| HpyAV | CCTTC | 1 cut(s) | 585 |
| HpyCH4V | TGCA | 7 cut(s) | 14, 74, 132, 158, 229, 474, 481 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 68, 235 |
| HpyF3I | CTNAG | 3 cut(s) | 99, 336, 379 |
| Hsp92II | CATG | 1 cut(s) | 349 |
| HspAI | GCGC | 1 cut(s) | 183 |
| Kzo9I | GATC | 4 cut(s) | 191, 252, 442, 594 |
| LpnPI | CCDG | 5 cut(s) | 111, 165, 192, 291, 431 |
| Lsp1109I | GCAGC | 3 cut(s) | 58, 80, 447 |
| LweI | GCATC | 1 cut(s) | 115 |
| MaeI | CTAG | 2 cut(s) | 266, 296 |
| MaeIII | GTNAC | 2 cut(s) | 42, 186 |
| MalI | GATC | 4 cut(s) | 193, 254, 444, 596 |
| MboI | GATC | 4 cut(s) | 191, 252, 442, 594 |
| MboII | GAAGA | 3 cut(s) | 134, 233, 529 |
| MflI | RGATCY | 1 cut(s) | 594 |
| MluCI | AATT | 6 cut(s) | 107, 301, 351, 482, 512, 577 |
| MmeI | TCCRAC | 1 cut(s) | 545 |
| MnlI | CCTC | 9 cut(s) | 26, 94, 103, 186, 210, 262, 352, 404, 581 |
| Mph1103I | ATGCAT | 1 cut(s) | 160 |
| MseI | TTAA | 2 cut(s) | 105, 153 |
| MslI | CAYNNNNRTG | 1 cut(s) | 52 |
| MspA1I | CMGCKG | 1 cut(s) | 71 |
| MspR9I | CCNGG | 1 cut(s) | 180 |
| Mva1269I | GAATGC | 1 cut(s) | 483 |
| MvaI | CCWGG | 1 cut(s) | 180 |
| MvnI | CGCG | 1 cut(s) | 185 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 68, 235 |
| NdeII | GATC | 4 cut(s) | 191, 252, 442, 594 |
| NlaIII | CATG | 1 cut(s) | 349 |
| NlaIV | GGNNCC | 1 cut(s) | 596 |
| NmuCI | GTSAC | 1 cut(s) | 42 |
| NsiI | ATGCAT | 1 cut(s) | 160 |
| PctI | GAATGC | 1 cut(s) | 483 |
| PfeI | GAWTC | 4 cut(s) | 79, 383, 493, 615 |
| PkrI | GCNGC | 5 cut(s) | 70, 73, 240, 462, 465 |
| PshAI | GACNNNNGTC | 1 cut(s) | 40 |
| PshBI | ATTAAT | 1 cut(s) | 153 |
| Psp6I | CCWGG | 1 cut(s) | 178 |
| PspGI | CCWGG | 1 cut(s) | 178 |
| PspN4I | GGNNCC | 1 cut(s) | 596 |
| PstI | CTGCAG | 1 cut(s) | 476 |
| PstNI | CAGNNNCTG | 1 cut(s) | 618 |
| PsuI | RGATCY | 1 cut(s) | 594 |
| PvuII | CAGCTG | 1 cut(s) | 71 |
| RseI | CAYNNNNRTG | 1 cut(s) | 52 |
| SaqAI | TTAA | 2 cut(s) | 105, 153 |
| SatI | GCNGC | 5 cut(s) | 69, 72, 239, 461, 464 |
| Sau3AI | GATC | 4 cut(s) | 191, 252, 442, 594 |
| ScrFI | CCNGG | 1 cut(s) | 180 |
| SfaNI | GCATC | 1 cut(s) | 115 |
| SfcI | CTRYAG | 1 cut(s) | 472 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 52 |
| SpeI | ACTAGT | 1 cut(s) | 295 |
| Sse9I | AATT | 6 cut(s) | 107, 301, 351, 482, 512, 577 |
| SsiI | CCGC | 2 cut(s) | 239, 463 |
| SspMI | CTAG | 2 cut(s) | 266, 296 |
| StyD4I | CCNGG | 1 cut(s) | 178 |
| TaqI | TCGA | 2 cut(s) | 202, 417 |
| TaqII | GACCGA | 1 cut(s) | 45 |
| TasI | AATT | 6 cut(s) | 107, 301, 351, 482, 512, 577 |
| TauI | GCSGC | 2 cut(s) | 241, 466 |
| TfiI | GAWTC | 4 cut(s) | 79, 383, 493, 615 |
| Tru1I | TTAA | 2 cut(s) | 105, 153 |
| Tru9I | TTAA | 2 cut(s) | 105, 153 |
| TseFI | GTSAC | 1 cut(s) | 42 |
| TseI | GCWGC | 3 cut(s) | 68, 71, 460 |
| Tsp45I | GTSAC | 1 cut(s) | 42 |
| TspDTI | ATGAA | 1 cut(s) | 71 |
| TspGWI | ACGGA | 2 cut(s) | 332, 600 |
| VspI | ATTAAT | 1 cut(s) | 153 |
| XspI | CTAG | 2 cut(s) | 266, 296 |
| Zsp2I | ATGCAT | 1 cut(s) | 160 |