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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccIII | TCCGGA | 1 cut(s) | 280 |
| AciI | CCGC | 9 cut(s) | 43, 59, 66, 94, 97, 100, 131, 164, 173 |
| AclWI | GGATC | 2 cut(s) | 73, 300 |
| AcoI | YGGCCR | 1 cut(s) | 13 |
| AcuI | CTGAAG | 1 cut(s) | 366 |
| AcyI | GRCGYC | 1 cut(s) | 30 |
| AgsI | TTSAA | 3 cut(s) | 206, 340, 433 |
| AluBI | AGCT | 2 cut(s) | 125, 380 |
| AluI | AGCT | 2 cut(s) | 125, 380 |
| AlwI | GGATC | 2 cut(s) | 73, 300 |
| Aor13HI | TCCGGA | 1 cut(s) | 280 |
| AoxI | GGCC | 2 cut(s) | 13, 151 |
| ApeKI | GCWGC | 1 cut(s) | 183 |
| AspS9I | GGNCC | 2 cut(s) | 151, 156 |
| AsuC2I | CCSGG | 1 cut(s) | 47 |
| AsuII | TTCGAA | 1 cut(s) | 355 |
| AvaII | GGWCC | 1 cut(s) | 156 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 305, 337 |
| BbvI | GCAGC | 1 cut(s) | 170 |
| BcnI | CCSGG | 1 cut(s) | 47 |
| BisI | GCNGC | 3 cut(s) | 95, 98, 184 |
| BlsI | GCNGC | 3 cut(s) | 96, 99, 185 |
| Bme1390I | CCNGG | 1 cut(s) | 47 |
| Bme18I | GGWCC | 1 cut(s) | 156 |
| BmgT120I | GGNCC | 2 cut(s) | 151, 156 |
| BmiI | GGNNCC | 2 cut(s) | 72, 152 |
| BmrFI | CCNGG | 1 cut(s) | 47 |
| BmsI | GCATC | 1 cut(s) | 8 |
| Bpu14I | TTCGAA | 1 cut(s) | 355 |
| BpuMI | CCSGG | 1 cut(s) | 47 |
| BsaHI | GRCGYC | 1 cut(s) | 30 |
| BsaJI | CCNNGG | 2 cut(s) | 116, 146 |
| BsaWI | WCCGGW | 3 cut(s) | 81, 158, 280 |
| Bse118I | RCCGGY | 1 cut(s) | 153 |
| BseAI | TCCGGA | 1 cut(s) | 280 |
| BseDI | CCNNGG | 2 cut(s) | 116, 146 |
| BseGI | GGATG | 1 cut(s) | 223 |
| BseRI | GAGGAG | 3 cut(s) | 182, 407, 410 |
| BseX3I | CGGCCG | 1 cut(s) | 13 |
| BseXI | GCAGC | 1 cut(s) | 170 |
| BsgI | GTGCAG | 1 cut(s) | 109 |
| Bsh1285I | CGRYCG | 1 cut(s) | 16 |
| BshFI | GGCC | 2 cut(s) | 15, 153 |
| BsiEI | CGRYCG | 1 cut(s) | 16 |
| BsiSI | CCGG | 5 cut(s) | 46, 82, 154, 159, 281 |
| BsnI | GGCC | 2 cut(s) | 15, 153 |
| Bsp119I | TTCGAA | 1 cut(s) | 355 |
| Bsp13I | TCCGGA | 1 cut(s) | 280 |
| Bsp143I | GATC | 2 cut(s) | 78, 292 |
| BspACI | CCGC | 9 cut(s) | 43, 59, 66, 94, 97, 100, 131, 164, 173 |
| BspANI | GGCC | 2 cut(s) | 15, 153 |
| BspEI | TCCGGA | 1 cut(s) | 280 |
| BspLI | GGNNCC | 2 cut(s) | 72, 152 |
| BspPI | GGATC | 2 cut(s) | 73, 300 |
| BspT104I | TTCGAA | 1 cut(s) | 355 |
| BsrFI | RCCGGY | 1 cut(s) | 153 |
| BssAI | RCCGGY | 1 cut(s) | 153 |
| BssECI | CCNNGG | 2 cut(s) | 116, 146 |
| BssMI | GATC | 2 cut(s) | 78, 292 |
| BssNI | GRCGYC | 1 cut(s) | 30 |
| Bst4CI | ACNGT | 2 cut(s) | 277, 415 |
| Bst6I | CTCTTC | 2 cut(s) | 240, 282 |
| BstACI | GRCGYC | 1 cut(s) | 30 |
| BstBI | TTCGAA | 1 cut(s) | 355 |
| BstC8I | GCNNGC | 2 cut(s) | 92, 138 |
| BstDSI | CCRYGG | 1 cut(s) | 116 |
| BstF5I | GGATG | 1 cut(s) | 223 |
| BstKTI | GATC | 2 cut(s) | 81, 295 |
| BstMBI | GATC | 2 cut(s) | 78, 292 |
| BstMCI | CGRYCG | 1 cut(s) | 16 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 27, 137, 170 |
| BstSCI | CCNGG | 1 cut(s) | 45 |
| BstV1I | GCAGC | 1 cut(s) | 170 |
| BstZI | CGGCCG | 1 cut(s) | 13 |
| BsuRI | GGCC | 2 cut(s) | 15, 153 |
| BtgI | CCRYGG | 1 cut(s) | 116 |
| BtsCI | GGATG | 1 cut(s) | 223 |
| Cac8I | GCNNGC | 2 cut(s) | 92, 138 |
| Cfr10I | RCCGGY | 1 cut(s) | 153 |
| Cfr13I | GGNCC | 2 cut(s) | 151, 156 |
| CpoI | CGGWCCG | 1 cut(s) | 156 |
| CseI | GACGC | 1 cut(s) | 19 |
| CspI | CGGWCCG | 1 cut(s) | 156 |
| CviAII | CATG | 1 cut(s) | 211 |
| CviJI | RGCY | 6 cut(s) | 15, 73, 125, 153, 183, 380 |
| CviKI_1 | RGCY | 6 cut(s) | 15, 73, 125, 153, 183, 380 |
| DpnI | GATC | 2 cut(s) | 80, 294 |
| DpnII | GATC | 2 cut(s) | 78, 292 |
| EaeI | YGGCCR | 1 cut(s) | 13 |
| EagI | CGGCCG | 1 cut(s) | 13 |
| Eam1104I | CTCTTC | 2 cut(s) | 240, 282 |
| EarI | CTCTTC | 2 cut(s) | 240, 282 |
| EciI | GGCGGA | 2 cut(s) | 32, 74 |
| EclXI | CGGCCG | 1 cut(s) | 13 |
| Eco47I | GGWCC | 1 cut(s) | 156 |
| Eco52I | CGGCCG | 1 cut(s) | 13 |
| Eco57I | CTGAAG | 1 cut(s) | 366 |
| FaeI | CATG | 1 cut(s) | 214 |
| FaiI | YATR | 3 cut(s) | 212, 408, 410 |
| FatI | CATG | 1 cut(s) | 210 |
| FauI | CCCGC | 4 cut(s) | 59, 93, 124, 166 |
| Fnu4HI | GCNGC | 3 cut(s) | 95, 98, 184 |
| FokI | GGATG | 1 cut(s) | 230 |
| Fsp4HI | GCNGC | 3 cut(s) | 95, 98, 184 |
| GluI | GCNGC | 3 cut(s) | 95, 98, 184 |
| HaeIII | GGCC | 2 cut(s) | 15, 153 |
| HapII | CCGG | 5 cut(s) | 46, 82, 154, 159, 281 |
| HgaI | GACGC | 1 cut(s) | 19 |
| Hin1I | GRCGYC | 1 cut(s) | 30 |
| Hin1II | CATG | 1 cut(s) | 214 |
| HinfI | GANTC | 3 cut(s) | 142, 230, 236 |
| HpaII | CCGG | 5 cut(s) | 46, 82, 154, 159, 281 |
| Hpy188I | TCNGA | 5 cut(s) | 147, 195, 235, 241, 385 |
| Hpy188III | TCNNGA | 1 cut(s) | 281 |
| Hpy99I | CGWCG | 1 cut(s) | 29 |
| HpyAV | CCTTC | 1 cut(s) | 84 |
| HpyCH4III | ACNGT | 2 cut(s) | 277, 415 |
| HpyCH4IV | ACGT | 1 cut(s) | 24 |
| HpyCH4V | TGCA | 3 cut(s) | 21, 90, 186 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 27, 137, 170 |
| HpySE526I | ACGT | 1 cut(s) | 24 |
| Hsp92I | GRCGYC | 1 cut(s) | 30 |
| Hsp92II | CATG | 1 cut(s) | 214 |
| Kpn2I | TCCGGA | 1 cut(s) | 280 |
| Kzo9I | GATC | 2 cut(s) | 78, 292 |
| LmnI | GCTCC | 2 cut(s) | 70, 122 |
| LpnPI | CCDG | 6 cut(s) | 59, 76, 95, 167, 172, 294 |
| Lsp1109I | GCAGC | 1 cut(s) | 170 |
| LweI | GCATC | 1 cut(s) | 8 |
| MaeII | ACGT | 1 cut(s) | 24 |
| MalI | GATC | 2 cut(s) | 80, 294 |
| MboI | GATC | 2 cut(s) | 78, 292 |
| MboII | GAAGA | 5 cut(s) | 257, 296, 299, 322, 325 |
| MluCI | AATT | 2 cut(s) | 298, 427 |
| MroI | TCCGGA | 1 cut(s) | 280 |
| MspI | CCGG | 5 cut(s) | 46, 82, 154, 159, 281 |
| MspR9I | CCNGG | 1 cut(s) | 47 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 27, 137, 170 |
| NciI | CCSGG | 1 cut(s) | 47 |
| NdeII | GATC | 2 cut(s) | 78, 292 |
| NlaIII | CATG | 1 cut(s) | 214 |
| NlaIV | GGNNCC | 2 cut(s) | 72, 152 |
| NspV | TTCGAA | 1 cut(s) | 355 |
| PfeI | GAWTC | 3 cut(s) | 142, 230, 236 |
| PkrI | GCNGC | 3 cut(s) | 96, 99, 185 |
| PspN4I | GGNNCC | 2 cut(s) | 72, 152 |
| PspPI | GGNCC | 2 cut(s) | 151, 156 |
| Rsr2I | CGGWCCG | 1 cut(s) | 156 |
| RsrII | CGGWCCG | 1 cut(s) | 156 |
| SatI | GCNGC | 3 cut(s) | 95, 98, 184 |
| Sau3AI | GATC | 2 cut(s) | 78, 292 |
| Sau96I | GGNCC | 2 cut(s) | 151, 156 |
| ScrFI | CCNGG | 1 cut(s) | 47 |
| SetI | ASST | 5 cut(s) | 27, 39, 127, 181, 382 |
| SfaNI | GCATC | 1 cut(s) | 8 |
| SfuI | TTCGAA | 1 cut(s) | 355 |
| SinI | GGWCC | 1 cut(s) | 156 |
| Sse9I | AATT | 2 cut(s) | 298, 427 |
| SsiI | CCGC | 9 cut(s) | 43, 59, 66, 94, 97, 100, 131, 164, 173 |
| StyD4I | CCNGG | 1 cut(s) | 45 |
| TaaI | ACNGT | 2 cut(s) | 277, 415 |
| TaiI | ACGT | 1 cut(s) | 27 |
| TaqI | TCGA | 3 cut(s) | 77, 330, 355 |
| TasI | AATT | 2 cut(s) | 298, 427 |
| TauI | GCSGC | 2 cut(s) | 97, 100 |
| TfiI | GAWTC | 3 cut(s) | 142, 230, 236 |
| TseI | GCWGC | 1 cut(s) | 183 |
| TspDTI | ATGAA | 2 cut(s) | 279, 323 |
| VpaK11BI | GGWCC | 1 cut(s) | 156 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 123 |