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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 774 |
| AciI | CCGC | 1 cut(s) | 432 |
| AclWI | GGATC | 1 cut(s) | 442 |
| AcsI | RAATTY | 3 cut(s) | 390, 717, 739 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 774 |
| AgsI | TTSAA | 4 cut(s) | 88, 482, 709, 722 |
| AjiI | CACGTC | 1 cut(s) | 263 |
| AjnI | CCWGG | 2 cut(s) | 265, 616 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 102, 134 |
| AluBI | AGCT | 3 cut(s) | 55, 76, 500 |
| AluI | AGCT | 3 cut(s) | 55, 76, 500 |
| AlwI | GGATC | 1 cut(s) | 442 |
| Ama87I | CYCGRG | 1 cut(s) | 56 |
| AoxI | GGCC | 1 cut(s) | 330 |
| ApeKI | GCWGC | 1 cut(s) | 500 |
| ApoI | RAATTY | 3 cut(s) | 390, 717, 739 |
| AspS9I | GGNCC | 1 cut(s) | 331 |
| AsuHPI | GGTGA | 3 cut(s) | 281, 465, 507 |
| AvaI | CYCGRG | 1 cut(s) | 56 |
| AxyI | CCTNAGG | 1 cut(s) | 336 |
| BbvI | GCAGC | 1 cut(s) | 487 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 482, 516 |
| BciT130I | CCWGG | 2 cut(s) | 267, 618 |
| BfaI | CTAG | 1 cut(s) | 306 |
| BglII | AGATCT | 1 cut(s) | 749 |
| BisI | GCNGC | 1 cut(s) | 501 |
| BlsI | GCNGC | 1 cut(s) | 502 |
| Bme1390I | CCNGG | 2 cut(s) | 267, 618 |
| BmeT110I | CYCGRG | 1 cut(s) | 56 |
| BmgBI | CACGTC | 1 cut(s) | 263 |
| BmgT120I | GGNCC | 1 cut(s) | 331 |
| BmiI | GGNNCC | 1 cut(s) | 333 |
| BmrFI | CCNGG | 2 cut(s) | 267, 618 |
| BmsI | GCATC | 1 cut(s) | 544 |
| BoxI | GACNNNNGTC | 1 cut(s) | 469 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 170, 202 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 134, 164, 277, 307 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 774 |
| Bse1I | ACTGG | 1 cut(s) | 686 |
| Bse21I | CCTNAGG | 1 cut(s) | 336 |
| Bse3DI | GCAATG | 1 cut(s) | 31 |
| BseBI | CCWGG | 2 cut(s) | 267, 618 |
| BseGI | GGATG | 1 cut(s) | 718 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 774 |
| BseMI | GCAATG | 1 cut(s) | 31 |
| BseMII | CTCAG | 2 cut(s) | 228, 350 |
| BseNI | ACTGG | 1 cut(s) | 686 |
| BseRI | GAGGAG | 1 cut(s) | 116 |
| BseXI | GCAGC | 1 cut(s) | 487 |
| BsgI | GTGCAG | 1 cut(s) | 486 |
| BshFI | GGCC | 1 cut(s) | 332 |
| BsiHKCI | CYCGRG | 1 cut(s) | 56 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 774 |
| BsmI | GAATGC | 2 cut(s) | 488, 587 |
| BsnI | GGCC | 1 cut(s) | 332 |
| BsoBI | CYCGRG | 1 cut(s) | 56 |
| Bsp143I | GATC | 2 cut(s) | 434, 749 |
| BspACI | CCGC | 1 cut(s) | 432 |
| BspANI | GGCC | 1 cut(s) | 332 |
| BspCNI | CTCAG | 2 cut(s) | 229, 349 |
| BspLI | GGNNCC | 1 cut(s) | 333 |
| BspPI | GGATC | 1 cut(s) | 442 |
| BspQI | GCTCTTC | 1 cut(s) | 66 |
| BsrDI | GCAATG | 1 cut(s) | 31 |
| BsrI | ACTGG | 1 cut(s) | 686 |
| BssMI | GATC | 2 cut(s) | 434, 749 |
| Bst2UI | CCWGG | 2 cut(s) | 267, 618 |
| Bst6I | CTCTTC | 3 cut(s) | 24, 66, 129 |
| BstC8I | GCNNGC | 3 cut(s) | 359, 430, 559 |
| BstDEI | CTNAG | 3 cut(s) | 77, 237, 336 |
| BstF5I | GGATG | 1 cut(s) | 718 |
| BstKTI | GATC | 2 cut(s) | 437, 752 |
| BstMBI | GATC | 2 cut(s) | 434, 749 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 497 |
| BstNI | CCWGG | 2 cut(s) | 267, 618 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 469 |
| BstSCI | CCNGG | 2 cut(s) | 265, 616 |
| BstV1I | GCAGC | 1 cut(s) | 487 |
| BstX2I | RGATCY | 2 cut(s) | 434, 749 |
| BstYI | RGATCY | 2 cut(s) | 434, 749 |
| Bsu36I | CCTNAGG | 1 cut(s) | 336 |
| BsuRI | GGCC | 1 cut(s) | 332 |
| BtrI | CACGTC | 1 cut(s) | 263 |
| BtsCI | GGATG | 1 cut(s) | 718 |
| BtsIMutI | CAGTG | 1 cut(s) | 188 |
| Cac8I | GCNNGC | 3 cut(s) | 359, 430, 559 |
| Cfr13I | GGNCC | 1 cut(s) | 331 |
| CviAII | CATG | 1 cut(s) | 769 |
| CviJI | RGCY | 7 cut(s) | 55, 76, 185, 332, 346, 500, 616 |
| CviKI_1 | RGCY | 7 cut(s) | 55, 76, 185, 332, 346, 500, 616 |
| DdeI | CTNAG | 3 cut(s) | 77, 237, 336 |
| DpnI | GATC | 2 cut(s) | 436, 751 |
| DpnII | GATC | 2 cut(s) | 434, 749 |
| Eam1104I | CTCTTC | 3 cut(s) | 24, 66, 129 |
| EarI | CTCTTC | 3 cut(s) | 24, 66, 129 |
| Eco81I | CCTNAGG | 1 cut(s) | 336 |
| Eco88I | CYCGRG | 1 cut(s) | 56 |
| EcoO109I | RGGNCCY | 1 cut(s) | 331 |
| EcoRI | GAATTC | 1 cut(s) | 390 |
| EcoRII | CCWGG | 2 cut(s) | 265, 616 |
| EcoT22I | ATGCAT | 2 cut(s) | 589, 765 |
| FaeI | CATG | 1 cut(s) | 772 |
| FaiI | YATR | 9 cut(s) | 149, 221, 246, 656, 696, 761, 765, 770, 785 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 739, 771 |
| FatI | CATG | 1 cut(s) | 768 |
| Fnu4HI | GCNGC | 1 cut(s) | 501 |
| FokI | GGATG | 1 cut(s) | 725 |
| Fsp4HI | GCNGC | 1 cut(s) | 501 |
| FspBI | CTAG | 1 cut(s) | 306 |
| GluI | GCNGC | 1 cut(s) | 501 |
| HaeIII | GGCC | 1 cut(s) | 332 |
| Hin1II | CATG | 1 cut(s) | 772 |
| HinfI | GANTC | 3 cut(s) | 248, 281, 419 |
| HphI | GGTGA | 3 cut(s) | 281, 465, 507 |
| Hpy166II | GTNNAC | 1 cut(s) | 734 |
| Hpy188III | TCNNGA | 1 cut(s) | 338 |
| Hpy8I | GTNNAC | 1 cut(s) | 734 |
| HpyAV | CCTTC | 2 cut(s) | 133, 703 |
| HpyCH4IV | ACGT | 1 cut(s) | 262 |
| HpyCH4V | TGCA | 5 cut(s) | 172, 503, 561, 587, 763 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 497 |
| HpyF3I | CTNAG | 3 cut(s) | 77, 237, 336 |
| HpySE526I | ACGT | 1 cut(s) | 262 |
| Hsp92II | CATG | 1 cut(s) | 772 |
| Kzo9I | GATC | 2 cut(s) | 434, 749 |
| LguI | GCTCTTC | 1 cut(s) | 66 |
| LpnPI | CCDG | 7 cut(s) | 252, 279, 323, 380, 603, 630, 667 |
| Lsp1109I | GCAGC | 1 cut(s) | 487 |
| LweI | GCATC | 1 cut(s) | 544 |
| MaeI | CTAG | 1 cut(s) | 306 |
| MaeII | ACGT | 1 cut(s) | 262 |
| MaeIII | GTNAC | 1 cut(s) | 471 |
| MalI | GATC | 2 cut(s) | 436, 751 |
| MboI | GATC | 2 cut(s) | 434, 749 |
| MboII | GAAGA | 7 cut(s) | 41, 46, 80, 83, 146, 563, 759 |
| MfeI | CAATTG | 1 cut(s) | 209 |
| MflI | RGATCY | 2 cut(s) | 434, 749 |
| MluCI | AATT | 9 cut(s) | 41, 117, 174, 209, 390, 536, 717, 739, 793 |
| MlyI | GAGTC | 3 cut(s) | 257, 275, 413 |
| Mph1103I | ATGCAT | 2 cut(s) | 589, 765 |
| MseI | TTAA | 4 cut(s) | 195, 534, 582, 802 |
| MslI | CAYNNNNRTG | 2 cut(s) | 224, 481 |
| MspR9I | CCNGG | 2 cut(s) | 267, 618 |
| MunI | CAATTG | 1 cut(s) | 209 |
| Mva1269I | GAATGC | 2 cut(s) | 488, 587 |
| MvaI | CCWGG | 2 cut(s) | 267, 618 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 497 |
| NdeII | GATC | 2 cut(s) | 434, 749 |
| NlaIII | CATG | 1 cut(s) | 772 |
| NlaIV | GGNNCC | 1 cut(s) | 333 |
| NmuCI | GTSAC | 1 cut(s) | 471 |
| NsiI | ATGCAT | 2 cut(s) | 589, 765 |
| PaeR7I | CTCGAG | 1 cut(s) | 56 |
| PciSI | GCTCTTC | 1 cut(s) | 66 |
| PctI | GAATGC | 2 cut(s) | 488, 587 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 774 |
| PkrI | GCNGC | 1 cut(s) | 502 |
| PleI | GAGTC | 3 cut(s) | 256, 275, 413 |
| PpsI | GAGTC | 3 cut(s) | 256, 275, 413 |
| PshAI | GACNNNNGTC | 1 cut(s) | 469 |
| Psp6I | CCWGG | 2 cut(s) | 265, 616 |
| PspGI | CCWGG | 2 cut(s) | 265, 616 |
| PspN4I | GGNNCC | 1 cut(s) | 333 |
| PspPI | GGNCC | 1 cut(s) | 331 |
| PspXI | VCTCGAGB | 1 cut(s) | 56 |
| PsuI | RGATCY | 2 cut(s) | 434, 749 |
| RseI | CAYNNNNRTG | 2 cut(s) | 224, 481 |
| SapI | GCTCTTC | 1 cut(s) | 66 |
| SaqAI | TTAA | 4 cut(s) | 195, 534, 582, 802 |
| SatI | GCNGC | 1 cut(s) | 501 |
| Sau3AI | GATC | 2 cut(s) | 434, 749 |
| Sau96I | GGNCC | 1 cut(s) | 331 |
| SchI | GAGTC | 3 cut(s) | 257, 275, 413 |
| ScrFI | CCNGG | 2 cut(s) | 267, 618 |
| SfaNI | GCATC | 1 cut(s) | 544 |
| Sfr274I | CTCGAG | 1 cut(s) | 56 |
| SlaI | CTCGAG | 1 cut(s) | 56 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 224, 481 |
| SmlI | CTYRAG | 1 cut(s) | 56 |
| SmoI | CTYRAG | 1 cut(s) | 56 |
| Sse9I | AATT | 9 cut(s) | 41, 117, 174, 209, 390, 536, 717, 739, 793 |
| SsiI | CCGC | 1 cut(s) | 432 |
| SspI | AATATT | 1 cut(s) | 705 |
| SspMI | CTAG | 1 cut(s) | 306 |
| StyD4I | CCNGG | 2 cut(s) | 265, 616 |
| TaiI | ACGT | 1 cut(s) | 265 |
| TaqI | TCGA | 1 cut(s) | 57 |
| TasI | AATT | 9 cut(s) | 41, 117, 174, 209, 390, 536, 717, 739, 793 |
| Tru1I | TTAA | 4 cut(s) | 195, 534, 582, 802 |
| Tru9I | TTAA | 4 cut(s) | 195, 534, 582, 802 |
| TscAI | CASTG | 1 cut(s) | 195 |
| TseFI | GTSAC | 1 cut(s) | 471 |
| TseI | GCWGC | 1 cut(s) | 500 |
| Tsp45I | GTSAC | 1 cut(s) | 471 |
| TspDTI | ATGAA | 5 cut(s) | 42, 54, 500, 643, 729 |
| TspGWI | ACGGA | 1 cut(s) | 563 |
| TspRI | CASTG | 1 cut(s) | 195 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 774 |
| XapI | RAATTY | 3 cut(s) | 390, 717, 739 |
| XhoI | CTCGAG | 1 cut(s) | 56 |
| XspI | CTAG | 1 cut(s) | 306 |
| Zsp2I | ATGCAT | 2 cut(s) | 589, 765 |