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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 237 |
| AfaI | GTAC | 1 cut(s) | 349 |
| AgsI | TTSAA | 1 cut(s) | 536 |
| AjiI | CACGTC | 1 cut(s) | 110 |
| AjnI | CCWGG | 1 cut(s) | 112 |
| Alw26I | GTCTC | 1 cut(s) | 102 |
| Ama87I | CYCGRG | 1 cut(s) | 183 |
| AoxI | GGCC | 1 cut(s) | 177 |
| ApoI | RAATTY | 1 cut(s) | 237 |
| AseI | ATTAAT | 1 cut(s) | 495 |
| AspLEI | GCGC | 1 cut(s) | 279 |
| AspS9I | GGNCC | 1 cut(s) | 178 |
| AsuHPI | GGTGA | 4 cut(s) | 27, 128, 395, 464 |
| AvaI | CYCGRG | 1 cut(s) | 183 |
| BccI | CCATC | 1 cut(s) | 67 |
| BceAI | ACGGC | 1 cut(s) | 473 |
| BciT130I | CCWGG | 1 cut(s) | 114 |
| BcoDI | GTCTC | 1 cut(s) | 102 |
| BfaI | CTAG | 1 cut(s) | 153 |
| BfmI | CTRYAG | 1 cut(s) | 295 |
| Bme1390I | CCNGG | 1 cut(s) | 114 |
| BmeT110I | CYCGRG | 1 cut(s) | 183 |
| BmgBI | CACGTC | 1 cut(s) | 110 |
| BmgT120I | GGNCC | 1 cut(s) | 178 |
| BmiI | GGNNCC | 1 cut(s) | 180 |
| BmrFI | CCNGG | 1 cut(s) | 114 |
| BmsI | GCATC | 1 cut(s) | 288 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 17, 49 |
| BpuEI | CTTGAG | 1 cut(s) | 213 |
| BsaJI | CCNNGG | 2 cut(s) | 182, 317 |
| BsaWI | WCCGGW | 1 cut(s) | 463 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 124, 154 |
| BseBI | CCWGG | 1 cut(s) | 114 |
| BseDI | CCNNGG | 2 cut(s) | 182, 317 |
| BseGI | GGATG | 2 cut(s) | 279, 508 |
| BseRI | GAGGAG | 3 cut(s) | 126, 129, 482 |
| Bsh1285I | CGRYCG | 1 cut(s) | 464 |
| BshFI | GGCC | 1 cut(s) | 179 |
| BsiEI | CGRYCG | 1 cut(s) | 464 |
| BsiHKCI | CYCGRG | 1 cut(s) | 183 |
| BsiSI | CCGG | 2 cut(s) | 234, 464 |
| BslFI | GGGAC | 2 cut(s) | 200, 390 |
| BsmAI | GTCTC | 1 cut(s) | 102 |
| BsmFI | GGGAC | 2 cut(s) | 200, 390 |
| BsnI | GGCC | 1 cut(s) | 179 |
| BsoBI | CYCGRG | 1 cut(s) | 183 |
| BspANI | GGCC | 1 cut(s) | 179 |
| BspLI | GGNNCC | 1 cut(s) | 180 |
| BspMAI | CTGCAG | 1 cut(s) | 299 |
| BssECI | CCNNGG | 2 cut(s) | 182, 317 |
| Bst2UI | CCWGG | 1 cut(s) | 114 |
| Bst4CI | ACNGT | 1 cut(s) | 452 |
| Bst6I | CTCTTC | 1 cut(s) | 327 |
| BstC8I | GCNNGC | 1 cut(s) | 206 |
| BstF5I | GGATG | 2 cut(s) | 279, 508 |
| BstHHI | GCGC | 1 cut(s) | 279 |
| BstMAI | GTCTC | 1 cut(s) | 102 |
| BstMCI | CGRYCG | 1 cut(s) | 464 |
| BstNI | CCWGG | 1 cut(s) | 114 |
| BstSCI | CCNGG | 1 cut(s) | 112 |
| BstSFI | CTRYAG | 1 cut(s) | 295 |
| BsuRI | GGCC | 1 cut(s) | 179 |
| BtrI | CACGTC | 1 cut(s) | 110 |
| BtsCI | GGATG | 2 cut(s) | 279, 508 |
| Cac8I | GCNNGC | 1 cut(s) | 206 |
| CfoI | GCGC | 1 cut(s) | 279 |
| Cfr13I | GGNCC | 1 cut(s) | 178 |
| Csp6I | GTAC | 1 cut(s) | 348 |
| CviAII | CATG | 1 cut(s) | 482 |
| CviJI | RGCY | 4 cut(s) | 32, 179, 193, 233 |
| CviKI_1 | RGCY | 4 cut(s) | 32, 179, 193, 233 |
| CviQI | GTAC | 1 cut(s) | 348 |
| Eam1104I | CTCTTC | 1 cut(s) | 327 |
| EarI | CTCTTC | 1 cut(s) | 327 |
| Eco88I | CYCGRG | 1 cut(s) | 183 |
| EcoO109I | RGGNCCY | 1 cut(s) | 178 |
| EcoRI | GAATTC | 1 cut(s) | 237 |
| EcoRII | CCWGG | 1 cut(s) | 112 |
| FaeI | CATG | 1 cut(s) | 485 |
| FaiI | YATR | 4 cut(s) | 68, 93, 364, 483 |
| FaqI | GGGAC | 2 cut(s) | 200, 390 |
| FatI | CATG | 1 cut(s) | 481 |
| FokI | GGATG | 2 cut(s) | 266, 515 |
| FspBI | CTAG | 1 cut(s) | 153 |
| GlaI | GCGC | 1 cut(s) | 278 |
| HaeIII | GGCC | 1 cut(s) | 179 |
| HapII | CCGG | 2 cut(s) | 234, 464 |
| HhaI | GCGC | 1 cut(s) | 279 |
| Hin1II | CATG | 1 cut(s) | 485 |
| Hin6I | GCGC | 1 cut(s) | 277 |
| HinP1I | GCGC | 1 cut(s) | 277 |
| HinfI | GANTC | 2 cut(s) | 95, 306 |
| HpaII | CCGG | 2 cut(s) | 234, 464 |
| HphI | GGTGA | 4 cut(s) | 27, 128, 395, 464 |
| Hpy166II | GTNNAC | 1 cut(s) | 455 |
| Hpy188I | TCNGA | 3 cut(s) | 243, 284, 328 |
| Hpy188III | TCNNGA | 1 cut(s) | 185 |
| Hpy8I | GTNNAC | 1 cut(s) | 455 |
| Hpy99I | CGWCG | 1 cut(s) | 447 |
| HpyCH4III | ACNGT | 1 cut(s) | 452 |
| HpyCH4IV | ACGT | 1 cut(s) | 109 |
| HpyCH4V | TGCA | 1 cut(s) | 297 |
| HpySE526I | ACGT | 1 cut(s) | 109 |
| Hsp92II | CATG | 1 cut(s) | 485 |
| HspAI | GCGC | 1 cut(s) | 277 |
| LpnPI | CCDG | 5 cut(s) | 99, 126, 247, 356, 477 |
| LweI | GCATC | 1 cut(s) | 288 |
| MaeI | CTAG | 1 cut(s) | 153 |
| MaeII | ACGT | 1 cut(s) | 109 |
| MaeIII | GTNAC | 1 cut(s) | 538 |
| MboII | GAAGA | 1 cut(s) | 344 |
| MfeI | CAATTG | 1 cut(s) | 56 |
| MluCI | AATT | 3 cut(s) | 56, 237, 531 |
| MlyI | GAGTC | 2 cut(s) | 104, 300 |
| MseI | TTAA | 2 cut(s) | 42, 495 |
| MslI | CAYNNNNRTG | 1 cut(s) | 71 |
| MspI | CCGG | 2 cut(s) | 234, 464 |
| MspR9I | CCNGG | 1 cut(s) | 114 |
| MunI | CAATTG | 1 cut(s) | 56 |
| MvaI | CCWGG | 1 cut(s) | 114 |
| NlaIII | CATG | 1 cut(s) | 485 |
| NlaIV | GGNNCC | 1 cut(s) | 180 |
| PleI | GAGTC | 2 cut(s) | 103, 300 |
| PpsI | GAGTC | 2 cut(s) | 103, 300 |
| PshBI | ATTAAT | 1 cut(s) | 495 |
| Psp6I | CCWGG | 1 cut(s) | 112 |
| PspGI | CCWGG | 1 cut(s) | 112 |
| PspN4I | GGNNCC | 1 cut(s) | 180 |
| PspPI | GGNCC | 1 cut(s) | 178 |
| PstI | CTGCAG | 1 cut(s) | 299 |
| RsaI | GTAC | 1 cut(s) | 349 |
| RsaNI | GTAC | 1 cut(s) | 348 |
| RseI | CAYNNNNRTG | 1 cut(s) | 71 |
| SaqAI | TTAA | 2 cut(s) | 42, 495 |
| Sau96I | GGNCC | 1 cut(s) | 178 |
| SchI | GAGTC | 2 cut(s) | 104, 300 |
| ScrFI | CCNGG | 1 cut(s) | 114 |
| SetI | ASST | 6 cut(s) | 15, 112, 118, 154, 261, 385 |
| SfaNI | GCATC | 1 cut(s) | 288 |
| SfcI | CTRYAG | 1 cut(s) | 295 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 71 |
| SmlI | CTYRAG | 1 cut(s) | 228 |
| SmoI | CTYRAG | 1 cut(s) | 228 |
| Sse9I | AATT | 3 cut(s) | 56, 237, 531 |
| SspMI | CTAG | 1 cut(s) | 153 |
| StyD4I | CCNGG | 1 cut(s) | 112 |
| TaaI | ACNGT | 1 cut(s) | 452 |
| TaiI | ACGT | 1 cut(s) | 112 |
| TaqI | TCGA | 1 cut(s) | 304 |
| TaqII | GACCGA | 1 cut(s) | 442 |
| TasI | AATT | 3 cut(s) | 56, 237, 531 |
| TatI | WGTACW | 1 cut(s) | 347 |
| Tru1I | TTAA | 2 cut(s) | 42, 495 |
| Tru9I | TTAA | 2 cut(s) | 42, 495 |
| TspDTI | ATGAA | 1 cut(s) | 543 |
| VspI | ATTAAT | 1 cut(s) | 495 |
| XapI | RAATTY | 1 cut(s) | 237 |
| XspI | CTAG | 1 cut(s) | 153 |