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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 604 |
| AciI | CCGC | 1 cut(s) | 485 |
| AcsI | RAATTY | 1 cut(s) | 376 |
| AcuI | CTGAAG | 1 cut(s) | 41 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 803 |
| AgsI | TTSAA | 4 cut(s) | 271, 381, 677, 815 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 89, 121, 285, 317 |
| AluBI | AGCT | 5 cut(s) | 76, 161, 238, 695, 885 |
| AluI | AGCT | 5 cut(s) | 76, 161, 238, 695, 885 |
| Alw26I | GTCTC | 3 cut(s) | 5, 41, 182 |
| AoxI | GGCC | 1 cut(s) | 513 |
| ApeKI | GCWGC | 3 cut(s) | 635, 692, 695 |
| ApoI | RAATTY | 1 cut(s) | 376 |
| AspLEI | GCGC | 1 cut(s) | 617 |
| AspS9I | GGNCC | 1 cut(s) | 514 |
| AsuC2I | CCSGG | 1 cut(s) | 804 |
| AsuHPI | GGTGA | 4 cut(s) | 16, 464, 660, 702 |
| AxyI | CCTNAGG | 1 cut(s) | 723 |
| BbvCI | CCTCAGC | 1 cut(s) | 519 |
| BbvI | GCAGC | 3 cut(s) | 647, 682, 704 |
| BccI | CCATC | 2 cut(s) | 178, 403 |
| BcnI | CCSGG | 1 cut(s) | 804 |
| BcoDI | GTCTC | 3 cut(s) | 5, 41, 182 |
| BfaI | CTAG | 1 cut(s) | 489 |
| BfmI | CTRYAG | 1 cut(s) | 633 |
| BfuAI | ACCTGC | 1 cut(s) | 604 |
| BisI | GCNGC | 3 cut(s) | 636, 693, 696 |
| BlsI | GCNGC | 3 cut(s) | 637, 694, 697 |
| Bme1390I | CCNGG | 1 cut(s) | 804 |
| BmgT120I | GGNCC | 1 cut(s) | 514 |
| BmiI | GGNNCC | 1 cut(s) | 516 |
| BmrFI | CCNGG | 1 cut(s) | 804 |
| BmsI | GCATC | 3 cut(s) | 626, 638, 739 |
| BoxI | GACNNNNGTC | 1 cut(s) | 664 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 353, 385 |
| BpmI | CTGGAG | 1 cut(s) | 260 |
| Bpu10I | CCTNAGC | 1 cut(s) | 519 |
| BpuEI | CTTGAG | 1 cut(s) | 302 |
| BpuMI | CCSGG | 1 cut(s) | 804 |
| BsaJI | CCNNGG | 1 cut(s) | 655 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 808, 838 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 803 |
| Bse1I | ACTGG | 1 cut(s) | 144 |
| Bse21I | CCTNAGG | 1 cut(s) | 723 |
| BseDI | CCNNGG | 1 cut(s) | 655 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 803 |
| BseMII | CTCAG | 5 cut(s) | 411, 533, 578, 612, 714 |
| BseNI | ACTGG | 1 cut(s) | 144 |
| BseRI | GAGGAG | 2 cut(s) | 299, 462 |
| BseXI | GCAGC | 3 cut(s) | 647, 682, 704 |
| BsgI | GTGCAG | 1 cut(s) | 681 |
| Bsh1285I | CGRYCG | 1 cut(s) | 655 |
| BshFI | GGCC | 1 cut(s) | 515 |
| BsiEI | CGRYCG | 1 cut(s) | 655 |
| BsiSI | CCGG | 1 cut(s) | 803 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 803 |
| BsmAI | GTCTC | 3 cut(s) | 5, 41, 182 |
| BsmI | GAATGC | 1 cut(s) | 683 |
| BsnI | GGCC | 1 cut(s) | 515 |
| BspACI | CCGC | 1 cut(s) | 485 |
| BspANI | GGCC | 1 cut(s) | 515 |
| BspCNI | CTCAG | 5 cut(s) | 412, 532, 577, 613, 715 |
| BspLI | GGNNCC | 1 cut(s) | 516 |
| BspMAI | CTGCAG | 1 cut(s) | 637 |
| BspMI | ACCTGC | 1 cut(s) | 604 |
| BspQI | GCTCTTC | 2 cut(s) | 66, 249 |
| BsrI | ACTGG | 1 cut(s) | 144 |
| BssECI | CCNNGG | 1 cut(s) | 655 |
| Bst6I | CTCTTC | 4 cut(s) | 66, 207, 249, 312 |
| BstC8I | GCNNGC | 5 cut(s) | 542, 601, 613, 754, 855 |
| BstDEI | CTNAG | 6 cut(s) | 77, 420, 519, 564, 621, 723 |
| BstHHI | GCGC | 1 cut(s) | 617 |
| BstMAI | GTCTC | 3 cut(s) | 5, 41, 182 |
| BstMCI | CGRYCG | 1 cut(s) | 655 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 635, 692 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 664 |
| BstSCI | CCNGG | 1 cut(s) | 802 |
| BstSFI | CTRYAG | 1 cut(s) | 633 |
| BstV1I | GCAGC | 3 cut(s) | 647, 682, 704 |
| Bsu36I | CCTNAGG | 1 cut(s) | 723 |
| BsuRI | GGCC | 1 cut(s) | 515 |
| BveI | ACCTGC | 1 cut(s) | 604 |
| Cac8I | GCNNGC | 5 cut(s) | 542, 601, 613, 754, 855 |
| CfoI | GCGC | 1 cut(s) | 617 |
| Cfr13I | GGNCC | 1 cut(s) | 514 |
| CviAII | CATG | 1 cut(s) | 18 |
| CviJI | RGCY | 8 cut(s) | 76, 105, 161, 238, 515, 529, 695, 885 |
| CviKI_1 | RGCY | 8 cut(s) | 76, 105, 161, 238, 515, 529, 695, 885 |
| DdeI | CTNAG | 6 cut(s) | 77, 420, 519, 564, 621, 723 |
| Eam1104I | CTCTTC | 4 cut(s) | 66, 207, 249, 312 |
| EarI | CTCTTC | 4 cut(s) | 66, 207, 249, 312 |
| Eco57I | CTGAAG | 1 cut(s) | 41 |
| Eco81I | CCTNAGG | 1 cut(s) | 723 |
| EcoO109I | RGGNCCY | 1 cut(s) | 514 |
| EcoRI | GAATTC | 1 cut(s) | 376 |
| EcoT22I | ATGCAT | 1 cut(s) | 784 |
| FaeI | CATG | 1 cut(s) | 21 |
| FaiI | YATR | 5 cut(s) | 19, 121, 404, 447, 851 |
| FatI | CATG | 1 cut(s) | 17 |
| Fnu4HI | GCNGC | 3 cut(s) | 636, 693, 696 |
| Fsp4HI | GCNGC | 3 cut(s) | 636, 693, 696 |
| FspBI | CTAG | 1 cut(s) | 489 |
| GlaI | GCGC | 1 cut(s) | 616 |
| GluI | GCNGC | 3 cut(s) | 636, 693, 696 |
| GsuI | CTGGAG | 1 cut(s) | 260 |
| HaeIII | GGCC | 1 cut(s) | 515 |
| HapII | CCGG | 1 cut(s) | 803 |
| HhaI | GCGC | 1 cut(s) | 617 |
| Hin1II | CATG | 1 cut(s) | 21 |
| Hin6I | GCGC | 1 cut(s) | 615 |
| HinP1I | GCGC | 1 cut(s) | 615 |
| HinfI | GANTC | 2 cut(s) | 368, 464 |
| HpaII | CCGG | 1 cut(s) | 803 |
| HphI | GGTGA | 4 cut(s) | 16, 464, 660, 702 |
| Hpy188I | TCNGA | 2 cut(s) | 60, 622 |
| Hpy188III | TCNNGA | 2 cut(s) | 381, 722 |
| HpyAV | CCTTC | 3 cut(s) | 283, 316, 619 |
| HpyCH4V | TGCA | 6 cut(s) | 17, 635, 698, 756, 782, 853 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 635, 692 |
| HpyF3I | CTNAG | 6 cut(s) | 77, 420, 519, 564, 621, 723 |
| Hsp92II | CATG | 1 cut(s) | 21 |
| HspAI | GCGC | 1 cut(s) | 615 |
| LguI | GCTCTTC | 2 cut(s) | 66, 249 |
| LmnI | GCTCC | 1 cut(s) | 608 |
| Lsp1109I | GCAGC | 3 cut(s) | 647, 682, 704 |
| LweI | GCATC | 3 cut(s) | 626, 638, 739 |
| MaeI | CTAG | 1 cut(s) | 489 |
| MaeIII | GTNAC | 1 cut(s) | 666 |
| MluCI | AATT | 8 cut(s) | 41, 125, 224, 300, 357, 376, 392, 731 |
| MlyI | GAGTC | 2 cut(s) | 362, 458 |
| Mph1103I | ATGCAT | 1 cut(s) | 784 |
| MseI | TTAA | 3 cut(s) | 171, 729, 777 |
| MslI | CAYNNNNRTG | 3 cut(s) | 407, 450, 676 |
| MspA1I | CMGCKG | 1 cut(s) | 695 |
| MspI | CCGG | 1 cut(s) | 803 |
| MspR9I | CCNGG | 1 cut(s) | 804 |
| Mva1269I | GAATGC | 1 cut(s) | 683 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 635, 692 |
| NciI | CCSGG | 1 cut(s) | 804 |
| NlaIII | CATG | 1 cut(s) | 21 |
| NlaIV | GGNNCC | 1 cut(s) | 516 |
| NmuCI | GTSAC | 1 cut(s) | 666 |
| NsiI | ATGCAT | 1 cut(s) | 784 |
| PciSI | GCTCTTC | 2 cut(s) | 66, 249 |
| PctI | GAATGC | 1 cut(s) | 683 |
| PkrI | GCNGC | 3 cut(s) | 637, 694, 697 |
| PleI | GAGTC | 2 cut(s) | 362, 458 |
| PpsI | GAGTC | 2 cut(s) | 362, 458 |
| PshAI | GACNNNNGTC | 1 cut(s) | 664 |
| PspN4I | GGNNCC | 1 cut(s) | 516 |
| PspPI | GGNCC | 1 cut(s) | 514 |
| PstI | CTGCAG | 1 cut(s) | 637 |
| PvuII | CAGCTG | 1 cut(s) | 695 |
| RseI | CAYNNNNRTG | 3 cut(s) | 407, 450, 676 |
| SapI | GCTCTTC | 2 cut(s) | 66, 249 |
| SaqAI | TTAA | 3 cut(s) | 171, 729, 777 |
| SatI | GCNGC | 3 cut(s) | 636, 693, 696 |
| Sau96I | GGNCC | 1 cut(s) | 514 |
| SchI | GAGTC | 2 cut(s) | 362, 458 |
| ScrFI | CCNGG | 1 cut(s) | 804 |
| SfaNI | GCATC | 3 cut(s) | 626, 638, 739 |
| SfcI | CTRYAG | 1 cut(s) | 633 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 407, 450, 676 |
| SmlI | CTYRAG | 1 cut(s) | 281 |
| SmoI | CTYRAG | 1 cut(s) | 281 |
| Sse9I | AATT | 8 cut(s) | 41, 125, 224, 300, 357, 376, 392, 731 |
| SsiI | CCGC | 1 cut(s) | 485 |
| SspMI | CTAG | 1 cut(s) | 489 |
| StyD4I | CCNGG | 1 cut(s) | 802 |
| TaqII | GACCGA | 1 cut(s) | 669 |
| TasI | AATT | 8 cut(s) | 41, 125, 224, 300, 357, 376, 392, 731 |
| Tru1I | TTAA | 3 cut(s) | 171, 729, 777 |
| Tru9I | TTAA | 3 cut(s) | 171, 729, 777 |
| TseFI | GTSAC | 1 cut(s) | 666 |
| TseI | GCWGC | 3 cut(s) | 635, 692, 695 |
| Tsp45I | GTSAC | 1 cut(s) | 666 |
| TspDTI | ATGAA | 2 cut(s) | 225, 695 |
| TspGWI | ACGGA | 1 cut(s) | 758 |
| XapI | RAATTY | 1 cut(s) | 376 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 768 |
| XspI | CTAG | 1 cut(s) | 489 |
| Zsp2I | ATGCAT | 1 cut(s) | 784 |