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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 307, 539 |
| AcuI | CTGAAG | 2 cut(s) | 404, 419 |
| AfaI | GTAC | 1 cut(s) | 168 |
| AgeI | ACCGGT | 1 cut(s) | 368 |
| AgsI | TTSAA | 1 cut(s) | 359 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 404, 436, 467, 499 |
| AluBI | AGCT | 1 cut(s) | 454 |
| AluI | AGCT | 1 cut(s) | 454 |
| Alw26I | GTCTC | 3 cut(s) | 383, 419, 560 |
| AsiGI | ACCGGT | 1 cut(s) | 368 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 114 |
| AspS9I | GGNCC | 1 cut(s) | 541 |
| AsuHPI | GGTGA | 1 cut(s) | 383 |
| AvaII | GGWCC | 1 cut(s) | 541 |
| BauI | CACGAG | 1 cut(s) | 312 |
| BbsI | GAAGAC | 1 cut(s) | 158 |
| BccI | CCATC | 1 cut(s) | 556 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 286, 320 |
| BcoDI | GTCTC | 3 cut(s) | 383, 419, 560 |
| Bme18I | GGWCC | 1 cut(s) | 541 |
| BmgT120I | GGNCC | 1 cut(s) | 541 |
| BmsI | GCATC | 1 cut(s) | 267 |
| BpiI | GAAGAC | 1 cut(s) | 158 |
| BsaWI | WCCGGW | 1 cut(s) | 368 |
| Bse118I | RCCGGY | 1 cut(s) | 368 |
| Bse1I | ACTGG | 1 cut(s) | 522 |
| BseGI | GGATG | 1 cut(s) | 216 |
| BseNI | ACTGG | 1 cut(s) | 522 |
| BseYI | CCCAGC | 1 cut(s) | 231 |
| BshTI | ACCGGT | 1 cut(s) | 368 |
| BsiSI | CCGG | 1 cut(s) | 369 |
| BslFI | GGGAC | 1 cut(s) | 214 |
| BsmAI | GTCTC | 3 cut(s) | 383, 419, 560 |
| BsmBI | CGTCTC | 1 cut(s) | 560 |
| BsmFI | GGGAC | 1 cut(s) | 214 |
| BspACI | CCGC | 2 cut(s) | 307, 539 |
| BspQI | GCTCTTC | 1 cut(s) | 444 |
| BsrFI | RCCGGY | 1 cut(s) | 368 |
| BsrI | ACTGG | 1 cut(s) | 522 |
| BssAI | RCCGGY | 1 cut(s) | 368 |
| BssSI | CACGAG | 1 cut(s) | 312 |
| Bst2BI | CACGAG | 1 cut(s) | 312 |
| Bst6I | CTCTTC | 3 cut(s) | 212, 232, 444 |
| BstDEI | CTNAG | 2 cut(s) | 123, 455 |
| BstF5I | GGATG | 1 cut(s) | 216 |
| BstMAI | GTCTC | 3 cut(s) | 383, 419, 560 |
| BstNSI | RCATGY | 1 cut(s) | 382 |
| BstV2I | GAAGAC | 1 cut(s) | 158 |
| BtsCI | GGATG | 1 cut(s) | 216 |
| Cfr10I | RCCGGY | 1 cut(s) | 368 |
| Cfr13I | GGNCC | 1 cut(s) | 541 |
| Csp6I | GTAC | 1 cut(s) | 167 |
| CspAI | ACCGGT | 1 cut(s) | 368 |
| CviAII | CATG | 2 cut(s) | 379, 396 |
| CviJI | RGCY | 2 cut(s) | 454, 483 |
| CviKI_1 | RGCY | 2 cut(s) | 454, 483 |
| CviQI | GTAC | 1 cut(s) | 167 |
| DdeI | CTNAG | 2 cut(s) | 123, 455 |
| Eam1104I | CTCTTC | 3 cut(s) | 212, 232, 444 |
| EarI | CTCTTC | 3 cut(s) | 212, 232, 444 |
| Eco47I | GGWCC | 1 cut(s) | 541 |
| Eco57I | CTGAAG | 2 cut(s) | 404, 419 |
| Esp3I | CGTCTC | 1 cut(s) | 560 |
| FaeI | CATG | 2 cut(s) | 382, 399 |
| FaiI | YATR | 3 cut(s) | 251, 380, 397 |
| FaqI | GGGAC | 1 cut(s) | 214 |
| FatI | CATG | 2 cut(s) | 378, 395 |
| FokI | GGATG | 1 cut(s) | 223 |
| GsaI | CCCAGC | 1 cut(s) | 235 |
| HapII | CCGG | 1 cut(s) | 369 |
| Hin1II | CATG | 2 cut(s) | 382, 399 |
| HinfI | GANTC | 3 cut(s) | 10, 293, 298 |
| HpaII | CCGG | 1 cut(s) | 369 |
| HphI | GGTGA | 1 cut(s) | 383 |
| Hpy188I | TCNGA | 1 cut(s) | 438 |
| Hpy99I | CGWCG | 1 cut(s) | 193 |
| HpyAV | CCTTC | 2 cut(s) | 171, 379 |
| HpyCH4V | TGCA | 1 cut(s) | 395 |
| HpyF3I | CTNAG | 2 cut(s) | 123, 455 |
| Hsp92II | CATG | 2 cut(s) | 382, 399 |
| LguI | GCTCTTC | 1 cut(s) | 444 |
| LpnPI | CCDG | 4 cut(s) | 73, 245, 382, 503 |
| LweI | GCATC | 1 cut(s) | 267 |
| MluCI | AATT | 2 cut(s) | 419, 503 |
| MlyI | GAGTC | 1 cut(s) | 307 |
| MnlI | CCTC | 3 cut(s) | 186, 213, 277 |
| MroXI | GAANNNNTTC | 1 cut(s) | 114 |
| MseI | TTAA | 2 cut(s) | 269, 549 |
| MspI | CCGG | 1 cut(s) | 369 |
| NlaIII | CATG | 2 cut(s) | 382, 399 |
| NspI | RCATGY | 1 cut(s) | 382 |
| PciSI | GCTCTTC | 1 cut(s) | 444 |
| PdmI | GAANNNNTTC | 1 cut(s) | 114 |
| PfeI | GAWTC | 2 cut(s) | 10, 293 |
| PinAI | ACCGGT | 1 cut(s) | 368 |
| PleI | GAGTC | 1 cut(s) | 306 |
| PpsI | GAGTC | 1 cut(s) | 306 |
| PspFI | CCCAGC | 1 cut(s) | 231 |
| PspPI | GGNCC | 1 cut(s) | 541 |
| RsaI | GTAC | 1 cut(s) | 168 |
| RsaNI | GTAC | 1 cut(s) | 167 |
| SapI | GCTCTTC | 1 cut(s) | 444 |
| SaqAI | TTAA | 2 cut(s) | 269, 549 |
| Sau96I | GGNCC | 1 cut(s) | 541 |
| SchI | GAGTC | 1 cut(s) | 307 |
| SetI | ASST | 1 cut(s) | 456 |
| SfaNI | GCATC | 1 cut(s) | 267 |
| SinI | GGWCC | 1 cut(s) | 541 |
| Sse9I | AATT | 2 cut(s) | 419, 503 |
| SsiI | CCGC | 2 cut(s) | 307, 539 |
| SspI | AATATT | 1 cut(s) | 257 |
| TaqI | TCGA | 2 cut(s) | 188, 296 |
| TasI | AATT | 2 cut(s) | 419, 503 |
| TatI | WGTACW | 1 cut(s) | 166 |
| TfiI | GAWTC | 2 cut(s) | 10, 293 |
| Tru1I | TTAA | 2 cut(s) | 269, 549 |
| Tru9I | TTAA | 2 cut(s) | 269, 549 |
| TspDTI | ATGAA | 4 cut(s) | 17, 32, 120, 230 |
| VpaK11BI | GGWCC | 1 cut(s) | 541 |
| XceI | RCATGY | 1 cut(s) | 382 |
| XmnI | GAANNNNTTC | 1 cut(s) | 114 |