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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 310 |
| AcuI | CTGAAG | 2 cut(s) | 60, 159 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 374 |
| AgsI | TTSAA | 1 cut(s) | 374 |
| AjnI | CCWGG | 1 cut(s) | 71 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 456, 488, 518, 550 |
| AluBI | AGCT | 4 cut(s) | 38, 388, 505, 590 |
| AluI | AGCT | 4 cut(s) | 38, 388, 505, 590 |
| Alw26I | GTCTC | 3 cut(s) | 433, 471, 611 |
| ApeKI | GCWGC | 2 cut(s) | 318, 388 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 153 |
| AsuC2I | CCSGG | 1 cut(s) | 670 |
| AsuII | TTCGAA | 1 cut(s) | 488 |
| BbsI | GAAGAC | 2 cut(s) | 197, 638 |
| BbvI | GCAGC | 2 cut(s) | 305, 375 |
| BccI | CCATC | 1 cut(s) | 607 |
| BciT130I | CCWGG | 1 cut(s) | 73 |
| BcnI | CCSGG | 1 cut(s) | 670 |
| BcoDI | GTCTC | 3 cut(s) | 433, 471, 611 |
| BisI | GCNGC | 2 cut(s) | 319, 389 |
| BlsI | GCNGC | 2 cut(s) | 320, 390 |
| Bme1390I | CCNGG | 2 cut(s) | 73, 670 |
| BmrFI | CCNGG | 2 cut(s) | 73, 670 |
| BmsI | GCATC | 1 cut(s) | 413 |
| BpiI | GAAGAC | 2 cut(s) | 197, 638 |
| Bpu14I | TTCGAA | 1 cut(s) | 488 |
| BpuMI | CCSGG | 1 cut(s) | 670 |
| Bsa29I | ATCGAT | 1 cut(s) | 56 |
| BsaJI | CCNNGG | 1 cut(s) | 431 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 374 |
| Bse1I | ACTGG | 1 cut(s) | 573 |
| BseBI | CCWGG | 1 cut(s) | 73 |
| BseCI | ATCGAT | 1 cut(s) | 56 |
| BseDI | CCNNGG | 1 cut(s) | 431 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 374 |
| BseNI | ACTGG | 1 cut(s) | 573 |
| BseRI | GAGGAG | 1 cut(s) | 451 |
| BseXI | GCAGC | 2 cut(s) | 305, 375 |
| BseYI | CCCAGC | 1 cut(s) | 271 |
| BsgI | GTGCAG | 1 cut(s) | 304 |
| BshVI | ATCGAT | 1 cut(s) | 56 |
| BsiSI | CCGG | 1 cut(s) | 669 |
| BslFI | GGGAC | 2 cut(s) | 254, 391 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 374 |
| BsmAI | GTCTC | 3 cut(s) | 433, 471, 611 |
| BsmFI | GGGAC | 2 cut(s) | 254, 391 |
| BsmI | GAATGC | 1 cut(s) | 426 |
| Bsp119I | TTCGAA | 1 cut(s) | 488 |
| Bsp19I | CCATGG | 1 cut(s) | 431 |
| BspACI | CCGC | 1 cut(s) | 310 |
| BspDI | ATCGAT | 1 cut(s) | 56 |
| BspQI | GCTCTTC | 1 cut(s) | 495 |
| BspT104I | TTCGAA | 1 cut(s) | 488 |
| BsrI | ACTGG | 1 cut(s) | 573 |
| BssECI | CCNNGG | 1 cut(s) | 431 |
| BssT1I | CCWWGG | 1 cut(s) | 431 |
| Bst2UI | CCWGG | 1 cut(s) | 73 |
| Bst6I | CTCTTC | 5 cut(s) | 252, 291, 495, 636, 680 |
| BstBI | TTCGAA | 1 cut(s) | 488 |
| BstC8I | GCNNGC | 2 cut(s) | 330, 393 |
| BstDEI | CTNAG | 1 cut(s) | 506 |
| BstDSI | CCRYGG | 1 cut(s) | 431 |
| BstMAI | GTCTC | 3 cut(s) | 433, 471, 611 |
| BstNI | CCWGG | 1 cut(s) | 73 |
| BstSCI | CCNGG | 2 cut(s) | 71, 668 |
| BstV1I | GCAGC | 2 cut(s) | 305, 375 |
| BstV2I | GAAGAC | 2 cut(s) | 197, 638 |
| Bsu15I | ATCGAT | 1 cut(s) | 56 |
| BsuTUI | ATCGAT | 1 cut(s) | 56 |
| BtgI | CCRYGG | 1 cut(s) | 431 |
| Cac8I | GCNNGC | 2 cut(s) | 330, 393 |
| ClaI | ATCGAT | 1 cut(s) | 56 |
| CviAII | CATG | 2 cut(s) | 432, 446 |
| CviJI | RGCY | 6 cut(s) | 38, 388, 505, 534, 590, 668 |
| CviKI_1 | RGCY | 6 cut(s) | 38, 388, 505, 534, 590, 668 |
| DdeI | CTNAG | 1 cut(s) | 506 |
| Eam1104I | CTCTTC | 5 cut(s) | 252, 291, 495, 636, 680 |
| EarI | CTCTTC | 5 cut(s) | 252, 291, 495, 636, 680 |
| Eco130I | CCWWGG | 1 cut(s) | 431 |
| Eco57I | CTGAAG | 2 cut(s) | 60, 159 |
| EcoRII | CCWGG | 1 cut(s) | 71 |
| EcoT14I | CCWWGG | 1 cut(s) | 431 |
| EcoT22I | ATGCAT | 1 cut(s) | 428 |
| ErhI | CCWWGG | 1 cut(s) | 431 |
| FaeI | CATG | 2 cut(s) | 435, 449 |
| FaiI | YATR | 5 cut(s) | 23, 291, 306, 433, 447 |
| FaqI | GGGAC | 2 cut(s) | 254, 391 |
| FatI | CATG | 2 cut(s) | 431, 445 |
| Fnu4HI | GCNGC | 2 cut(s) | 319, 389 |
| Fsp4HI | GCNGC | 2 cut(s) | 319, 389 |
| GluI | GCNGC | 2 cut(s) | 319, 389 |
| GsaI | CCCAGC | 1 cut(s) | 275 |
| HapII | CCGG | 1 cut(s) | 669 |
| Hin1II | CATG | 2 cut(s) | 435, 449 |
| HinfI | GANTC | 2 cut(s) | 10, 53 |
| HpaII | CCGG | 1 cut(s) | 669 |
| Hpy99I | CGWCG | 1 cut(s) | 233 |
| HpyAV | CCTTC | 3 cut(s) | 22, 211, 368 |
| HpyCH4V | TGCA | 8 cut(s) | 161, 209, 321, 350, 395, 411, 426, 445 |
| HpyF3I | CTNAG | 1 cut(s) | 506 |
| Hsp92II | CATG | 2 cut(s) | 435, 449 |
| LguI | GCTCTTC | 1 cut(s) | 495 |
| LpnPI | CCDG | 5 cut(s) | 58, 85, 285, 554, 682 |
| Lsp1109I | GCAGC | 2 cut(s) | 305, 375 |
| LweI | GCATC | 1 cut(s) | 413 |
| MluCI | AATT | 4 cut(s) | 345, 471, 554, 653 |
| MnlI | CCTC | 6 cut(s) | 18, 226, 253, 292, 429, 666 |
| Mph1103I | ATGCAT | 1 cut(s) | 428 |
| MroXI | GAANNNNTTC | 1 cut(s) | 153 |
| MseI | TTAA | 1 cut(s) | 600 |
| MspI | CCGG | 1 cut(s) | 669 |
| MspR9I | CCNGG | 2 cut(s) | 73, 670 |
| Mva1269I | GAATGC | 1 cut(s) | 426 |
| MvaI | CCWGG | 1 cut(s) | 73 |
| NciI | CCSGG | 1 cut(s) | 670 |
| NcoI | CCATGG | 1 cut(s) | 431 |
| NlaIII | CATG | 2 cut(s) | 435, 449 |
| NsiI | ATGCAT | 1 cut(s) | 428 |
| NspV | TTCGAA | 1 cut(s) | 488 |
| PciSI | GCTCTTC | 1 cut(s) | 495 |
| PctI | GAATGC | 1 cut(s) | 426 |
| PdmI | GAANNNNTTC | 1 cut(s) | 153 |
| PfeI | GAWTC | 2 cut(s) | 10, 53 |
| PfoI | TCCNGGA | 1 cut(s) | 71 |
| PkrI | GCNGC | 2 cut(s) | 320, 390 |
| Psp6I | CCWGG | 1 cut(s) | 71 |
| PspFI | CCCAGC | 1 cut(s) | 271 |
| PspGI | CCWGG | 1 cut(s) | 71 |
| SapI | GCTCTTC | 1 cut(s) | 495 |
| SaqAI | TTAA | 1 cut(s) | 600 |
| SatI | GCNGC | 2 cut(s) | 319, 389 |
| ScrFI | CCNGG | 2 cut(s) | 73, 670 |
| SetI | ASST | 4 cut(s) | 40, 390, 507, 592 |
| SfaNI | GCATC | 1 cut(s) | 413 |
| SfuI | TTCGAA | 1 cut(s) | 488 |
| Sse9I | AATT | 4 cut(s) | 345, 471, 554, 653 |
| SsiI | CCGC | 1 cut(s) | 310 |
| StyD4I | CCNGG | 2 cut(s) | 71, 668 |
| StyI | CCWWGG | 1 cut(s) | 431 |
| TaqI | TCGA | 2 cut(s) | 56, 488 |
| TasI | AATT | 4 cut(s) | 345, 471, 554, 653 |
| TfiI | GAWTC | 2 cut(s) | 10, 53 |
| Tru1I | TTAA | 1 cut(s) | 600 |
| Tru9I | TTAA | 1 cut(s) | 600 |
| TseI | GCWGC | 2 cut(s) | 318, 388 |
| TspDTI | ATGAA | 6 cut(s) | 17, 60, 74, 159, 270, 654 |
| XmnI | GAANNNNTTC | 1 cut(s) | 153 |
| Zsp2I | ATGCAT | 1 cut(s) | 428 |