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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 626 |
| AclWI | GGATC | 1 cut(s) | 765 |
| AcsI | RAATTY | 2 cut(s) | 456, 585 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 332, 685 |
| AgsI | TTSAA | 5 cut(s) | 64, 173, 238, 560, 619 |
| AjiI | CACGTC | 1 cut(s) | 329 |
| AjnI | CCWGG | 1 cut(s) | 331 |
| AluBI | AGCT | 4 cut(s) | 31, 452, 578, 781 |
| AluI | AGCT | 4 cut(s) | 31, 452, 578, 781 |
| Alw26I | GTCTC | 1 cut(s) | 321 |
| AlwI | GGATC | 1 cut(s) | 765 |
| Ama87I | CYCGRG | 2 cut(s) | 402, 605 |
| AoxI | GGCC | 1 cut(s) | 396 |
| ApeKI | GCWGC | 1 cut(s) | 578 |
| ApoI | RAATTY | 2 cut(s) | 456, 585 |
| AspLEI | GCGC | 1 cut(s) | 501 |
| AspS9I | GGNCC | 1 cut(s) | 397 |
| AsuC2I | CCSGG | 1 cut(s) | 686 |
| AsuHPI | GGTGA | 5 cut(s) | 246, 347, 543, 585, 760 |
| AvaI | CYCGRG | 2 cut(s) | 402, 605 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 735, 768 |
| BanII | GRGCYC | 1 cut(s) | 691 |
| BbvI | GCAGC | 1 cut(s) | 565 |
| BccI | CCATC | 2 cut(s) | 109, 286 |
| BciT130I | CCWGG | 1 cut(s) | 333 |
| BcnI | CCSGG | 1 cut(s) | 686 |
| BcoDI | GTCTC | 1 cut(s) | 321 |
| BfaI | CTAG | 1 cut(s) | 372 |
| BisI | GCNGC | 1 cut(s) | 579 |
| BlsI | GCNGC | 1 cut(s) | 580 |
| Bme1390I | CCNGG | 2 cut(s) | 333, 686 |
| BmeT110I | CYCGRG | 2 cut(s) | 402, 605 |
| BmgBI | CACGTC | 1 cut(s) | 329 |
| BmgT120I | GGNCC | 1 cut(s) | 397 |
| BmiI | GGNNCC | 1 cut(s) | 399 |
| BmrFI | CCNGG | 2 cut(s) | 333, 686 |
| BmsI | GCATC | 2 cut(s) | 510, 622 |
| BoxI | GACNNNNGTC | 1 cut(s) | 547 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 146, 178, 236, 268 |
| BpmI | CTGGAG | 1 cut(s) | 53 |
| BpuEI | CTTGAG | 1 cut(s) | 432 |
| BpuMI | CCSGG | 1 cut(s) | 686 |
| BsaJI | CCNNGG | 3 cut(s) | 401, 538, 606 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 110, 140, 343, 373, 690, 720 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 332, 685 |
| BseBI | CCWGG | 1 cut(s) | 333 |
| BseDI | CCNNGG | 3 cut(s) | 401, 538, 606 |
| BseGI | GGATG | 3 cut(s) | 127, 214, 501 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 332, 685 |
| BseRI | GAGGAG | 2 cut(s) | 345, 348 |
| BseXI | GCAGC | 1 cut(s) | 565 |
| BsgI | GTGCAG | 1 cut(s) | 564 |
| BshFI | GGCC | 1 cut(s) | 398 |
| BsiHKCI | CYCGRG | 2 cut(s) | 402, 605 |
| BsiSI | CCGG | 1 cut(s) | 685 |
| BslFI | GGGAC | 1 cut(s) | 419 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 332, 685 |
| BsmAI | GTCTC | 1 cut(s) | 321 |
| BsmFI | GGGAC | 1 cut(s) | 419 |
| BsmI | GAATGC | 1 cut(s) | 566 |
| BsnI | GGCC | 1 cut(s) | 398 |
| BsoBI | CYCGRG | 2 cut(s) | 402, 605 |
| Bsp1286I | GDGCHC | 1 cut(s) | 691 |
| Bsp143I | GATC | 1 cut(s) | 757 |
| BspACI | CCGC | 1 cut(s) | 626 |
| BspANI | GGCC | 1 cut(s) | 398 |
| BspLI | GGNNCC | 1 cut(s) | 399 |
| BspPI | GGATC | 1 cut(s) | 765 |
| BssECI | CCNNGG | 3 cut(s) | 401, 538, 606 |
| BssMI | GATC | 1 cut(s) | 757 |
| Bst2UI | CCWGG | 1 cut(s) | 333 |
| Bst6I | CTCTTC | 3 cut(s) | 42, 105, 195 |
| BstC8I | GCNNGC | 5 cut(s) | 425, 497, 637, 737, 741 |
| BstEII | GGTNACC | 1 cut(s) | 549 |
| BstF5I | GGATG | 3 cut(s) | 127, 214, 501 |
| BstHHI | GCGC | 1 cut(s) | 501 |
| BstKTI | GATC | 1 cut(s) | 760 |
| BstMAI | GTCTC | 1 cut(s) | 321 |
| BstMBI | GATC | 1 cut(s) | 757 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 575, 632 |
| BstNI | CCWGG | 1 cut(s) | 333 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 547 |
| BstPI | GGTNACC | 1 cut(s) | 549 |
| BstSCI | CCNGG | 2 cut(s) | 331, 684 |
| BstV1I | GCAGC | 1 cut(s) | 565 |
| BsuRI | GGCC | 1 cut(s) | 398 |
| BtgZI | GCGATG | 1 cut(s) | 499 |
| BtrI | CACGTC | 1 cut(s) | 329 |
| BtsCI | GGATG | 3 cut(s) | 127, 214, 501 |
| Cac8I | GCNNGC | 5 cut(s) | 425, 497, 637, 737, 741 |
| CfoI | GCGC | 1 cut(s) | 501 |
| Cfr13I | GGNCC | 1 cut(s) | 397 |
| DpnI | GATC | 1 cut(s) | 759 |
| DpnII | GATC | 1 cut(s) | 757 |
| Eam1104I | CTCTTC | 3 cut(s) | 42, 105, 195 |
| EarI | CTCTTC | 3 cut(s) | 42, 105, 195 |
| EciI | GGCGGA | 1 cut(s) | 641 |
| Eco24I | GRGCYC | 1 cut(s) | 691 |
| Eco88I | CYCGRG | 2 cut(s) | 402, 605 |
| Eco91I | GGTNACC | 1 cut(s) | 549 |
| EcoO109I | RGGNCCY | 1 cut(s) | 397 |
| EcoO65I | GGTNACC | 1 cut(s) | 549 |
| EcoRI | GAATTC | 1 cut(s) | 456 |
| EcoRII | CCWGG | 1 cut(s) | 331 |
| EcoT22I | ATGCAT | 1 cut(s) | 666 |
| EcoT38I | GRGCYC | 1 cut(s) | 691 |
| FaiI | YATR | 4 cut(s) | 287, 312, 733, 765 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 765 |
| FaqI | GGGAC | 1 cut(s) | 419 |
| Fnu4HI | GCNGC | 1 cut(s) | 579 |
| FokI | GGATG | 3 cut(s) | 134, 221, 488 |
| FriOI | GRGCYC | 1 cut(s) | 691 |
| Fsp4HI | GCNGC | 1 cut(s) | 579 |
| FspBI | CTAG | 1 cut(s) | 372 |
| GlaI | GCGC | 1 cut(s) | 500 |
| GluI | GCNGC | 1 cut(s) | 579 |
| GsuI | CTGGAG | 1 cut(s) | 53 |
| HaeIII | GGCC | 1 cut(s) | 398 |
| HapII | CCGG | 1 cut(s) | 685 |
| HhaI | GCGC | 1 cut(s) | 501 |
| Hin6I | GCGC | 1 cut(s) | 499 |
| HinP1I | GCGC | 1 cut(s) | 499 |
| HinfI | GANTC | 2 cut(s) | 314, 527 |
| HpaII | CCGG | 1 cut(s) | 685 |
| HphI | GGTGA | 5 cut(s) | 246, 347, 543, 585, 760 |
| Hpy188I | TCNGA | 2 cut(s) | 506, 757 |
| Hpy188III | TCNNGA | 4 cut(s) | 77, 404, 461, 605 |
| HpyAV | CCTTC | 1 cut(s) | 199 |
| HpyCH4IV | ACGT | 1 cut(s) | 328 |
| HpyCH4V | TGCA | 4 cut(s) | 581, 639, 664, 735 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 575, 632 |
| HpySE526I | ACGT | 1 cut(s) | 328 |
| HspAI | GCGC | 1 cut(s) | 499 |
| Kzo9I | GATC | 1 cut(s) | 757 |
| LpnPI | CCDG | 6 cut(s) | 17, 318, 345, 438, 446, 698 |
| Lsp1109I | GCAGC | 1 cut(s) | 565 |
| LweI | GCATC | 2 cut(s) | 510, 622 |
| MaeI | CTAG | 1 cut(s) | 372 |
| MaeII | ACGT | 1 cut(s) | 328 |
| MaeIII | GTNAC | 2 cut(s) | 549, 692 |
| MalI | GATC | 1 cut(s) | 759 |
| MboI | GATC | 1 cut(s) | 757 |
| MboII | GAAGA | 8 cut(s) | 16, 21, 56, 59, 122, 212, 641, 739 |
| MfeI | CAATTG | 1 cut(s) | 275 |
| MhlI | GDGCHC | 1 cut(s) | 691 |
| MluCI | AATT | 8 cut(s) | 16, 93, 150, 183, 275, 456, 585, 614 |
| MlyI | GAGTC | 2 cut(s) | 323, 521 |
| Mph1103I | ATGCAT | 1 cut(s) | 666 |
| MseI | TTAA | 4 cut(s) | 218, 261, 612, 659 |
| MslI | CAYNNNNRTG | 2 cut(s) | 290, 559 |
| MspI | CCGG | 1 cut(s) | 685 |
| MspR9I | CCNGG | 2 cut(s) | 333, 686 |
| MunI | CAATTG | 1 cut(s) | 275 |
| Mva1269I | GAATGC | 1 cut(s) | 566 |
| MvaI | CCWGG | 1 cut(s) | 333 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 575, 632 |
| NciI | CCSGG | 1 cut(s) | 686 |
| NdeII | GATC | 1 cut(s) | 757 |
| NlaIV | GGNNCC | 1 cut(s) | 399 |
| NmuCI | GTSAC | 1 cut(s) | 549 |
| NsiI | ATGCAT | 1 cut(s) | 666 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 522 |
| PctI | GAATGC | 1 cut(s) | 566 |
| PkrI | GCNGC | 1 cut(s) | 580 |
| PleI | GAGTC | 2 cut(s) | 322, 521 |
| PpsI | GAGTC | 2 cut(s) | 322, 521 |
| PshAI | GACNNNNGTC | 1 cut(s) | 547 |
| Psp6I | CCWGG | 1 cut(s) | 331 |
| PspEI | GGTNACC | 1 cut(s) | 549 |
| PspGI | CCWGG | 1 cut(s) | 331 |
| PspN4I | GGNNCC | 1 cut(s) | 399 |
| PspPI | GGNCC | 1 cut(s) | 397 |
| RseI | CAYNNNNRTG | 2 cut(s) | 290, 559 |
| SaqAI | TTAA | 4 cut(s) | 218, 261, 612, 659 |
| SatI | GCNGC | 1 cut(s) | 579 |
| Sau3AI | GATC | 1 cut(s) | 757 |
| Sau96I | GGNCC | 1 cut(s) | 397 |
| SchI | GAGTC | 2 cut(s) | 323, 521 |
| ScrFI | CCNGG | 2 cut(s) | 333, 686 |
| SduI | GDGCHC | 1 cut(s) | 691 |
| SfaNI | GCATC | 2 cut(s) | 510, 622 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 290, 559 |
| SmlI | CTYRAG | 1 cut(s) | 447 |
| SmoI | CTYRAG | 1 cut(s) | 447 |
| Sse9I | AATT | 8 cut(s) | 16, 93, 150, 183, 275, 456, 585, 614 |
| SsiI | CCGC | 1 cut(s) | 626 |
| SspMI | CTAG | 1 cut(s) | 372 |
| StyD4I | CCNGG | 2 cut(s) | 331, 684 |
| TaiI | ACGT | 1 cut(s) | 331 |
| TaqII | GACCGA | 1 cut(s) | 537 |
| TasI | AATT | 8 cut(s) | 16, 93, 150, 183, 275, 456, 585, 614 |
| Tru1I | TTAA | 4 cut(s) | 218, 261, 612, 659 |
| Tru9I | TTAA | 4 cut(s) | 218, 261, 612, 659 |
| TseFI | GTSAC | 1 cut(s) | 549 |
| TseI | GCWGC | 1 cut(s) | 578 |
| Tsp45I | GTSAC | 1 cut(s) | 549 |
| TspDTI | ATGAA | 2 cut(s) | 17, 578 |
| TspGWI | ACGGA | 2 cut(s) | 209, 709 |
| XapI | RAATTY | 2 cut(s) | 456, 585 |
| XspI | CTAG | 1 cut(s) | 372 |
| Zsp2I | ATGCAT | 1 cut(s) | 666 |