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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 3 cut(s) | 152, 163, 475 |
| AcsI | RAATTY | 3 cut(s) | 62, 137, 451 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 91, 188, 410 |
| AgsI | TTSAA | 4 cut(s) | 165, 255, 526, 728 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 623, 655 |
| AluBI | AGCT | 2 cut(s) | 293, 498 |
| AluI | AGCT | 2 cut(s) | 293, 498 |
| AlwI | GGATC | 3 cut(s) | 152, 163, 475 |
| AoxI | GGCC | 1 cut(s) | 93 |
| ApeKI | GCWGC | 3 cut(s) | 546, 560, 563 |
| ApoI | RAATTY | 3 cut(s) | 62, 137, 451 |
| AseI | ATTAAT | 1 cut(s) | 368 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 137, 421 |
| AspA2I | CCTAGG | 1 cut(s) | 172 |
| AspLEI | GCGC | 1 cut(s) | 307 |
| AspS9I | GGNCC | 1 cut(s) | 199 |
| AsuHPI | GGTGA | 1 cut(s) | 203 |
| AvaII | GGWCC | 1 cut(s) | 199 |
| AvrII | CCTAGG | 1 cut(s) | 172 |
| BbvI | GCAGC | 3 cut(s) | 558, 572, 575 |
| BccI | CCATC | 1 cut(s) | 628 |
| BceAI | ACGGC | 1 cut(s) | 266 |
| BfaI | CTAG | 1 cut(s) | 173 |
| BfmI | CTRYAG | 1 cut(s) | 89 |
| BisI | GCNGC | 3 cut(s) | 547, 561, 564 |
| BlnI | CCTAGG | 1 cut(s) | 172 |
| BlsI | GCNGC | 3 cut(s) | 548, 562, 565 |
| Bme18I | GGWCC | 1 cut(s) | 199 |
| BmgT120I | GGNCC | 1 cut(s) | 199 |
| BmiI | GGNNCC | 1 cut(s) | 201 |
| BmrI | ACTGGG | 1 cut(s) | 487 |
| BmsI | GCATC | 3 cut(s) | 10, 292, 575 |
| BmuI | ACTGGG | 1 cut(s) | 487 |
| BoxI | GACNNNNGTC | 1 cut(s) | 699 |
| BsaAI | YACGTR | 1 cut(s) | 436 |
| BsaJI | CCNNGG | 3 cut(s) | 28, 172, 282 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 623, 653 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 91, 188, 410 |
| Bse1I | ACTGG | 1 cut(s) | 482 |
| BseDI | CCNNGG | 3 cut(s) | 28, 172, 282 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 91, 188, 410 |
| BseMII | CTCAG | 1 cut(s) | 204 |
| BseNI | ACTGG | 1 cut(s) | 482 |
| BseRI | GAGGAG | 2 cut(s) | 59, 74 |
| BseXI | GCAGC | 3 cut(s) | 558, 572, 575 |
| BsgI | GTGCAG | 2 cut(s) | 25, 571 |
| BshFI | GGCC | 1 cut(s) | 95 |
| BslFI | GGGAC | 2 cut(s) | 185, 443 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 91, 188, 410 |
| BsmFI | GGGAC | 2 cut(s) | 185, 443 |
| BsmI | GAATGC | 2 cut(s) | 23, 183 |
| BsnI | GGCC | 1 cut(s) | 95 |
| Bsp143I | GATC | 4 cut(s) | 144, 168, 462, 467 |
| Bsp19I | CCATGG | 1 cut(s) | 28 |
| BspANI | GGCC | 1 cut(s) | 95 |
| BspCNI | CTCAG | 1 cut(s) | 205 |
| BspLI | GGNNCC | 1 cut(s) | 201 |
| BspMAI | CTGCAG | 1 cut(s) | 93 |
| BspPI | GGATC | 3 cut(s) | 152, 163, 475 |
| BsrI | ACTGG | 1 cut(s) | 482 |
| BssECI | CCNNGG | 3 cut(s) | 28, 172, 282 |
| BssMI | GATC | 4 cut(s) | 144, 168, 462, 467 |
| BssT1I | CCWWGG | 2 cut(s) | 28, 172 |
| Bst4CI | ACNGT | 3 cut(s) | 592, 639, 670 |
| Bst6I | CTCTTC | 2 cut(s) | 122, 327 |
| BstBAI | YACGTR | 1 cut(s) | 436 |
| BstC8I | GCNNGC | 2 cut(s) | 93, 445 |
| BstDEI | CTNAG | 1 cut(s) | 213 |
| BstDSI | CCRYGG | 2 cut(s) | 28, 282 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 89 |
| BstHHI | GCGC | 1 cut(s) | 307 |
| BstKTI | GATC | 4 cut(s) | 147, 171, 465, 470 |
| BstMBI | GATC | 4 cut(s) | 144, 168, 462, 467 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 304 |
| BstNSI | RCATGY | 1 cut(s) | 664 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 699 |
| BstSFI | CTRYAG | 1 cut(s) | 89 |
| BstSNI | TACGTA | 1 cut(s) | 436 |
| BstV1I | GCAGC | 3 cut(s) | 558, 572, 575 |
| BstX2I | RGATCY | 3 cut(s) | 144, 168, 467 |
| BstYI | RGATCY | 3 cut(s) | 144, 168, 467 |
| BsuRI | GGCC | 1 cut(s) | 95 |
| BtgI | CCRYGG | 2 cut(s) | 28, 282 |
| BtsI | GCAGTG | 1 cut(s) | 583 |
| BtsIMutI | CAGTG | 3 cut(s) | 583, 597, 675 |
| Cac8I | GCNNGC | 2 cut(s) | 93, 445 |
| CfoI | GCGC | 1 cut(s) | 307 |
| Cfr13I | GGNCC | 1 cut(s) | 199 |
| CviAII | CATG | 4 cut(s) | 29, 104, 426, 661 |
| CviJI | RGCY | 8 cut(s) | 56, 95, 281, 293, 443, 498, 549, 648 |
| CviKI_1 | RGCY | 8 cut(s) | 56, 95, 281, 293, 443, 498, 549, 648 |
| DdeI | CTNAG | 1 cut(s) | 213 |
| DpnI | GATC | 4 cut(s) | 146, 170, 464, 469 |
| DpnII | GATC | 4 cut(s) | 144, 168, 462, 467 |
| Eam1104I | CTCTTC | 2 cut(s) | 122, 327 |
| EarI | CTCTTC | 2 cut(s) | 122, 327 |
| Eco105I | TACGTA | 1 cut(s) | 436 |
| Eco130I | CCWWGG | 2 cut(s) | 28, 172 |
| Eco147I | AGGCCT | 1 cut(s) | 95 |
| Eco47I | GGWCC | 1 cut(s) | 199 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 89 |
| EcoT14I | CCWWGG | 2 cut(s) | 28, 172 |
| EcoT22I | ATGCAT | 1 cut(s) | 25 |
| ErhI | CCWWGG | 2 cut(s) | 28, 172 |
| FaeI | CATG | 4 cut(s) | 32, 107, 429, 664 |
| FaqI | GGGAC | 2 cut(s) | 185, 443 |
| FatI | CATG | 4 cut(s) | 28, 103, 425, 660 |
| Fnu4HI | GCNGC | 3 cut(s) | 547, 561, 564 |
| Fsp4HI | GCNGC | 3 cut(s) | 547, 561, 564 |
| FspBI | CTAG | 1 cut(s) | 173 |
| GlaI | GCGC | 1 cut(s) | 306 |
| GluI | GCNGC | 3 cut(s) | 547, 561, 564 |
| HaeIII | GGCC | 1 cut(s) | 95 |
| HhaI | GCGC | 1 cut(s) | 307 |
| Hin1II | CATG | 4 cut(s) | 32, 107, 429, 664 |
| Hin6I | GCGC | 1 cut(s) | 305 |
| HinP1I | GCGC | 1 cut(s) | 305 |
| HindIII | AAGCTT | 1 cut(s) | 496 |
| HinfI | GANTC | 3 cut(s) | 316, 539, 632 |
| HphI | GGTGA | 1 cut(s) | 203 |
| Hpy166II | GTNNAC | 1 cut(s) | 658 |
| Hpy188I | TCNGA | 4 cut(s) | 214, 232, 467, 705 |
| Hpy188III | TCNNGA | 3 cut(s) | 142, 148, 643 |
| Hpy8I | GTNNAC | 1 cut(s) | 658 |
| HpyAV | CCTTC | 1 cut(s) | 340 |
| HpyCH4III | ACNGT | 3 cut(s) | 592, 639, 670 |
| HpyCH4IV | ACGT | 1 cut(s) | 435 |
| HpyCH4V | TGCA | 7 cut(s) | 6, 23, 91, 221, 298, 410, 588 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 304 |
| HpyF3I | CTNAG | 1 cut(s) | 213 |
| HpySE526I | ACGT | 1 cut(s) | 435 |
| Hsp92II | CATG | 4 cut(s) | 32, 107, 429, 664 |
| HspAI | GCGC | 1 cut(s) | 305 |
| Kzo9I | GATC | 4 cut(s) | 144, 168, 462, 467 |
| Lsp1109I | GCAGC | 3 cut(s) | 558, 572, 575 |
| LweI | GCATC | 3 cut(s) | 10, 292, 575 |
| MaeI | CTAG | 1 cut(s) | 173 |
| MaeII | ACGT | 1 cut(s) | 435 |
| MaeIII | GTNAC | 2 cut(s) | 32, 633 |
| MalI | GATC | 4 cut(s) | 146, 170, 464, 469 |
| MboI | GATC | 4 cut(s) | 144, 168, 462, 467 |
| MboII | GAAGA | 6 cut(s) | 109, 112, 145, 344, 355, 611 |
| MflI | RGATCY | 3 cut(s) | 144, 168, 467 |
| MluCI | AATT | 7 cut(s) | 62, 137, 267, 420, 451, 603, 723 |
| MlyI | GAGTC | 2 cut(s) | 548, 641 |
| MmeI | TCCRAC | 3 cut(s) | 255, 344, 472 |
| MnlI | CCTC | 9 cut(s) | 37, 95, 125, 208, 322, 407, 591, 627, 659 |
| Mph1103I | ATGCAT | 1 cut(s) | 25 |
| MroXI | GAANNNNTTC | 2 cut(s) | 137, 421 |
| MseI | TTAA | 4 cut(s) | 270, 368, 503, 606 |
| MslI | CAYNNNNRTG | 1 cut(s) | 108 |
| Mva1269I | GAATGC | 2 cut(s) | 23, 183 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 304 |
| NcoI | CCATGG | 1 cut(s) | 28 |
| NdeII | GATC | 4 cut(s) | 144, 168, 462, 467 |
| NlaIII | CATG | 4 cut(s) | 32, 107, 429, 664 |
| NlaIV | GGNNCC | 1 cut(s) | 201 |
| NmuCI | GTSAC | 1 cut(s) | 633 |
| NsiI | ATGCAT | 1 cut(s) | 25 |
| NspI | RCATGY | 1 cut(s) | 664 |
| PceI | AGGCCT | 1 cut(s) | 95 |
| PctI | GAATGC | 2 cut(s) | 23, 183 |
| PdmI | GAANNNNTTC | 2 cut(s) | 137, 421 |
| PfeI | GAWTC | 1 cut(s) | 316 |
| PflFI | GACNNNGTC | 1 cut(s) | 236 |
| PkrI | GCNGC | 3 cut(s) | 548, 562, 565 |
| PleI | GAGTC | 2 cut(s) | 547, 640 |
| PpsI | GAGTC | 2 cut(s) | 547, 640 |
| Ppu21I | YACGTR | 1 cut(s) | 436 |
| PshAI | GACNNNNGTC | 1 cut(s) | 699 |
| PshBI | ATTAAT | 1 cut(s) | 368 |
| PspN4I | GGNNCC | 1 cut(s) | 201 |
| PspPI | GGNCC | 1 cut(s) | 199 |
| PstI | CTGCAG | 1 cut(s) | 93 |
| PsuI | RGATCY | 3 cut(s) | 144, 168, 467 |
| PsyI | GACNNNGTC | 1 cut(s) | 236 |
| RseI | CAYNNNNRTG | 1 cut(s) | 108 |
| SaqAI | TTAA | 4 cut(s) | 270, 368, 503, 606 |
| SatI | GCNGC | 3 cut(s) | 547, 561, 564 |
| Sau3AI | GATC | 4 cut(s) | 144, 168, 462, 467 |
| Sau96I | GGNCC | 1 cut(s) | 199 |
| SbfI | CCTGCAGG | 1 cut(s) | 93 |
| SchI | GAGTC | 2 cut(s) | 548, 641 |
| SdaI | CCTGCAGG | 1 cut(s) | 93 |
| SetI | ASST | 7 cut(s) | 117, 193, 295, 438, 500, 583, 619 |
| SfaNI | GCATC | 3 cut(s) | 10, 292, 575 |
| SfcI | CTRYAG | 1 cut(s) | 89 |
| SinI | GGWCC | 1 cut(s) | 199 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 108 |
| SnaBI | TACGTA | 1 cut(s) | 436 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 93 |
| Sse9I | AATT | 7 cut(s) | 62, 137, 267, 420, 451, 603, 723 |
| SseBI | AGGCCT | 1 cut(s) | 95 |
| SspI | AATATT | 1 cut(s) | 13 |
| SspMI | CTAG | 1 cut(s) | 173 |
| StuI | AGGCCT | 1 cut(s) | 95 |
| StyI | CCWWGG | 2 cut(s) | 28, 172 |
| TaaI | ACNGT | 3 cut(s) | 592, 639, 670 |
| TaiI | ACGT | 1 cut(s) | 438 |
| TaqI | TCGA | 3 cut(s) | 18, 149, 611 |
| TasI | AATT | 7 cut(s) | 62, 137, 267, 420, 451, 603, 723 |
| TfiI | GAWTC | 1 cut(s) | 316 |
| Tru1I | TTAA | 4 cut(s) | 270, 368, 503, 606 |
| Tru9I | TTAA | 4 cut(s) | 270, 368, 503, 606 |
| TscAI | CASTG | 3 cut(s) | 590, 597, 675 |
| TseFI | GTSAC | 1 cut(s) | 633 |
| TseI | GCWGC | 3 cut(s) | 546, 560, 563 |
| Tsp45I | GTSAC | 1 cut(s) | 633 |
| TspDTI | ATGAA | 4 cut(s) | 64, 120, 376, 414 |
| TspRI | CASTG | 3 cut(s) | 590, 597, 675 |
| Tth111I | GACNNNGTC | 1 cut(s) | 236 |
| VpaK11BI | GGWCC | 1 cut(s) | 199 |
| VspI | ATTAAT | 1 cut(s) | 368 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 89 |
| XapI | RAATTY | 3 cut(s) | 62, 137, 451 |
| XceI | RCATGY | 1 cut(s) | 664 |
| XmaJI | CCTAGG | 1 cut(s) | 172 |
| XmnI | GAANNNNTTC | 2 cut(s) | 137, 421 |
| XspI | CTAG | 1 cut(s) | 173 |
| Zsp2I | ATGCAT | 1 cut(s) | 25 |