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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 3 cut(s) | 11, 131, 224 |
| AcsI | RAATTY | 1 cut(s) | 43 |
| AfaI | GTAC | 1 cut(s) | 298 |
| AflIII | ACRYGT | 1 cut(s) | 246 |
| AgsI | TTSAA | 1 cut(s) | 278 |
| AluBI | AGCT | 1 cut(s) | 114 |
| AluI | AGCT | 1 cut(s) | 114 |
| ApeKI | GCWGC | 2 cut(s) | 100, 111 |
| ApoI | RAATTY | 1 cut(s) | 43 |
| AsiSI | GCGATCGC | 1 cut(s) | 83 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 285 |
| AspS9I | GGNCC | 1 cut(s) | 71 |
| AsuHPI | GGTGA | 2 cut(s) | 16, 120 |
| AvaII | GGWCC | 1 cut(s) | 71 |
| BanII | GRGCYC | 1 cut(s) | 199 |
| BbsI | GAAGAC | 1 cut(s) | 328 |
| BbvI | GCAGC | 2 cut(s) | 87, 123 |
| BccI | CCATC | 1 cut(s) | 176 |
| BfaI | CTAG | 1 cut(s) | 192 |
| BfmI | CTRYAG | 1 cut(s) | 270 |
| BisI | GCNGC | 2 cut(s) | 101, 112 |
| BlsI | GCNGC | 2 cut(s) | 102, 113 |
| Bme18I | GGWCC | 1 cut(s) | 71 |
| BmgT120I | GGNCC | 1 cut(s) | 71 |
| BmiI | GGNNCC | 1 cut(s) | 73 |
| BmsI | GCATC | 1 cut(s) | 65 |
| BpiI | GAAGAC | 1 cut(s) | 328 |
| BsaWI | WCCGGW | 1 cut(s) | 327 |
| BseXI | GCAGC | 2 cut(s) | 87, 123 |
| Bsh1285I | CGRYCG | 2 cut(s) | 83, 91 |
| BsiEI | CGRYCG | 2 cut(s) | 83, 91 |
| BsiSI | CCGG | 1 cut(s) | 328 |
| BslFI | GGGAC | 1 cut(s) | 57 |
| BsmFI | GGGAC | 1 cut(s) | 57 |
| BsmI | GAATGC | 2 cut(s) | 13, 102 |
| Bsp1286I | GDGCHC | 1 cut(s) | 199 |
| Bsp143I | GATC | 1 cut(s) | 80 |
| BspACI | CCGC | 3 cut(s) | 11, 131, 224 |
| BspLI | GGNNCC | 1 cut(s) | 73 |
| BssMI | GATC | 1 cut(s) | 80 |
| Bst4CI | ACNGT | 5 cut(s) | 89, 141, 187, 204, 384 |
| BstC8I | GCNNGC | 3 cut(s) | 105, 109, 135 |
| BstKTI | GATC | 1 cut(s) | 83 |
| BstMBI | GATC | 1 cut(s) | 80 |
| BstMCI | CGRYCG | 2 cut(s) | 83, 91 |
| BstNSI | RCATGY | 2 cut(s) | 214, 250 |
| BstSFI | CTRYAG | 1 cut(s) | 270 |
| BstV1I | GCAGC | 2 cut(s) | 87, 123 |
| BstV2I | GAAGAC | 1 cut(s) | 328 |
| Cac8I | GCNNGC | 3 cut(s) | 105, 109, 135 |
| Cfr13I | GGNCC | 1 cut(s) | 71 |
| Csp6I | GTAC | 1 cut(s) | 297 |
| CviAII | CATG | 3 cut(s) | 76, 211, 247 |
| CviJI | RGCY | 3 cut(s) | 114, 197, 242 |
| CviKI_1 | RGCY | 3 cut(s) | 114, 197, 242 |
| CviQI | GTAC | 1 cut(s) | 297 |
| DpnI | GATC | 1 cut(s) | 82 |
| DpnII | GATC | 1 cut(s) | 80 |
| EciI | GGCGGA | 1 cut(s) | 239 |
| Eco24I | GRGCYC | 1 cut(s) | 199 |
| Eco47I | GGWCC | 1 cut(s) | 71 |
| EcoT38I | GRGCYC | 1 cut(s) | 199 |
| FaeI | CATG | 3 cut(s) | 79, 214, 250 |
| FaiI | YATR | 6 cut(s) | 77, 212, 218, 248, 272, 308 |
| FaqI | GGGAC | 1 cut(s) | 57 |
| FatI | CATG | 3 cut(s) | 75, 210, 246 |
| Fnu4HI | GCNGC | 2 cut(s) | 101, 112 |
| FriOI | GRGCYC | 1 cut(s) | 199 |
| Fsp4HI | GCNGC | 2 cut(s) | 101, 112 |
| FspBI | CTAG | 1 cut(s) | 192 |
| GluI | GCNGC | 2 cut(s) | 101, 112 |
| HapII | CCGG | 1 cut(s) | 328 |
| Hin1II | CATG | 3 cut(s) | 79, 214, 250 |
| HinfI | GANTC | 1 cut(s) | 180 |
| HpaII | CCGG | 1 cut(s) | 328 |
| HphI | GGTGA | 2 cut(s) | 16, 120 |
| Hpy166II | GTNNAC | 1 cut(s) | 353 |
| Hpy188I | TCNGA | 1 cut(s) | 255 |
| Hpy188III | TCNNGA | 1 cut(s) | 192 |
| Hpy8I | GTNNAC | 1 cut(s) | 353 |
| HpyAV | CCTTC | 1 cut(s) | 58 |
| HpyCH4III | ACNGT | 5 cut(s) | 89, 141, 187, 204, 384 |
| HpyCH4V | TGCA | 3 cut(s) | 56, 103, 360 |
| Hsp92II | CATG | 3 cut(s) | 79, 214, 250 |
| Kzo9I | GATC | 1 cut(s) | 80 |
| LmnI | GCTCC | 1 cut(s) | 330 |
| LpnPI | CCDG | 1 cut(s) | 341 |
| Lsp1109I | GCAGC | 2 cut(s) | 87, 123 |
| LweI | GCATC | 1 cut(s) | 65 |
| MaeI | CTAG | 1 cut(s) | 192 |
| MaeIII | GTNAC | 2 cut(s) | 34, 204 |
| MalI | GATC | 1 cut(s) | 82 |
| MboI | GATC | 1 cut(s) | 80 |
| MboII | GAAGA | 5 cut(s) | 105, 159, 290, 330, 333 |
| MhlI | GDGCHC | 1 cut(s) | 199 |
| MluCI | AATT | 1 cut(s) | 43 |
| MlyI | GAGTC | 1 cut(s) | 174 |
| MnlI | CCTC | 2 cut(s) | 7, 136 |
| MroXI | GAANNNNTTC | 1 cut(s) | 285 |
| MslI | CAYNNNNRTG | 1 cut(s) | 382 |
| MspI | CCGG | 1 cut(s) | 328 |
| Mva1269I | GAATGC | 2 cut(s) | 13, 102 |
| NdeII | GATC | 1 cut(s) | 80 |
| NlaIII | CATG | 3 cut(s) | 79, 214, 250 |
| NlaIV | GGNNCC | 1 cut(s) | 73 |
| NmuCI | GTSAC | 2 cut(s) | 34, 204 |
| NspI | RCATGY | 2 cut(s) | 214, 250 |
| PciI | ACATGT | 1 cut(s) | 246 |
| PctI | GAATGC | 2 cut(s) | 13, 102 |
| PdmI | GAANNNNTTC | 1 cut(s) | 285 |
| PkrI | GCNGC | 2 cut(s) | 102, 113 |
| Ple19I | CGATCG | 1 cut(s) | 83 |
| PleI | GAGTC | 1 cut(s) | 174 |
| PpsI | GAGTC | 1 cut(s) | 174 |
| PscI | ACATGT | 1 cut(s) | 246 |
| PspN4I | GGNNCC | 1 cut(s) | 73 |
| PspPI | GGNCC | 1 cut(s) | 71 |
| PvuI | CGATCG | 1 cut(s) | 83 |
| RgaI | GCGATCGC | 1 cut(s) | 83 |
| RsaI | GTAC | 1 cut(s) | 298 |
| RsaNI | GTAC | 1 cut(s) | 297 |
| RseI | CAYNNNNRTG | 1 cut(s) | 382 |
| SatI | GCNGC | 2 cut(s) | 101, 112 |
| Sau3AI | GATC | 1 cut(s) | 80 |
| Sau96I | GGNCC | 1 cut(s) | 71 |
| SchI | GAGTC | 1 cut(s) | 174 |
| SduI | GDGCHC | 1 cut(s) | 199 |
| SetI | ASST | 4 cut(s) | 69, 116, 128, 367 |
| SfaAI | GCGATCGC | 1 cut(s) | 83 |
| SfaNI | GCATC | 1 cut(s) | 65 |
| SfcI | CTRYAG | 1 cut(s) | 270 |
| SgfI | GCGATCGC | 1 cut(s) | 83 |
| SinI | GGWCC | 1 cut(s) | 71 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 382 |
| Sse9I | AATT | 1 cut(s) | 43 |
| SsiI | CCGC | 3 cut(s) | 11, 131, 224 |
| SspMI | CTAG | 1 cut(s) | 192 |
| TaaI | ACNGT | 5 cut(s) | 89, 141, 187, 204, 384 |
| TaqI | TCGA | 3 cut(s) | 62, 91, 159 |
| TaqII | GACCGA | 2 cut(s) | 53, 59 |
| TasI | AATT | 1 cut(s) | 43 |
| TseFI | GTSAC | 2 cut(s) | 34, 204 |
| TseI | GCWGC | 2 cut(s) | 100, 111 |
| Tsp45I | GTSAC | 2 cut(s) | 34, 204 |
| VpaK11BI | GGWCC | 1 cut(s) | 71 |
| XapI | RAATTY | 1 cut(s) | 43 |
| XbaI | TCTAGA | 1 cut(s) | 191 |
| XceI | RCATGY | 2 cut(s) | 214, 250 |
| XmnI | GAANNNNTTC | 1 cut(s) | 285 |
| XspI | CTAG | 1 cut(s) | 192 |